##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	557770
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99258834286534	33.0	31.0	34.0	30.0	34.0
2	32.15398282446169	33.0	31.0	34.0	30.0	34.0
3	32.067529626907145	33.0	31.0	34.0	30.0	34.0
4	35.784873693457875	37.0	35.0	37.0	35.0	37.0
5	31.2906413037632	37.0	35.0	37.0	0.0	37.0
6	33.48195134195098	37.0	35.0	37.0	17.0	37.0
7	29.408489162199473	35.0	32.0	37.0	0.0	37.0
8	32.50849454076053	35.0	33.0	37.0	17.0	37.0
9	36.453046954838015	39.0	35.0	39.0	32.0	39.0
10	36.83343134266813	38.0	37.0	39.0	33.0	39.0
11	37.013485845420156	39.0	37.0	39.0	34.0	39.0
12	37.03670867920469	39.0	37.0	39.0	33.0	39.0
13	36.871482869283035	39.0	37.0	39.0	33.0	39.0
14	38.12850995930222	40.0	37.0	41.0	33.0	41.0
15	38.216106997507936	40.0	37.0	41.0	33.0	41.0
16	38.27559029707586	40.0	37.0	41.0	34.0	41.0
17	38.24956702583502	40.0	37.0	41.0	34.0	41.0
18	38.16912705954067	40.0	37.0	41.0	33.0	41.0
19	38.080101116947844	40.0	37.0	41.0	34.0	41.0
20	38.019196084407554	40.0	37.0	41.0	33.0	41.0
21	37.918111407928	40.0	37.0	41.0	33.0	41.0
22	37.92076303852843	40.0	37.0	41.0	33.0	41.0
23	37.860643275902255	40.0	36.0	41.0	33.0	41.0
24	37.79392043315345	40.0	36.0	41.0	33.0	41.0
25	37.65160370762142	40.0	36.0	41.0	33.0	41.0
26	37.48743030281299	40.0	36.0	41.0	33.0	41.0
27	37.44149380569052	40.0	36.0	41.0	33.0	41.0
28	37.32634777775786	39.0	36.0	41.0	32.0	41.0
29	37.288093658676516	39.0	36.0	41.0	32.0	41.0
30	37.17136812664718	39.0	35.0	41.0	32.0	41.0
31	37.03997884432651	39.0	35.0	41.0	31.0	41.0
32	36.94471914947021	39.0	35.0	41.0	31.0	41.0
33	36.793780590566	39.0	35.0	41.0	31.0	41.0
34	36.65454936622622	39.0	35.0	41.0	30.0	41.0
35	36.5586980296538	39.0	35.0	41.0	30.0	41.0
36	36.44254441795005	39.0	35.0	41.0	30.0	41.0
37	36.358493644333684	39.0	35.0	41.0	30.0	41.0
38	36.24727038026427	39.0	35.0	40.0	30.0	41.0
39	36.21460100041236	39.0	35.0	40.0	29.0	41.0
40	36.07210319665812	39.0	35.0	40.0	29.0	41.0
41	35.95116266561486	39.0	35.0	40.0	29.0	41.0
42	35.91979489753841	39.0	35.0	40.0	29.0	41.0
43	35.781564085554976	38.0	35.0	40.0	28.0	41.0
44	35.5594976423974	38.0	35.0	40.0	27.0	41.0
45	35.47257292432364	38.0	34.0	40.0	27.0	41.0
46	35.41612492604479	38.0	34.0	40.0	27.0	41.0
47	35.31528407766642	38.0	34.0	40.0	26.0	41.0
48	35.21357548810442	38.0	34.0	40.0	26.0	41.0
49	35.081587392652885	38.0	34.0	40.0	26.0	41.0
50	34.930102013374686	38.0	34.0	40.0	26.0	41.0
51	33.06802445452426	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	14.0
9	28.0
10	51.0
11	32.0
12	29.0
13	30.0
14	45.0
15	54.0
16	82.0
17	147.0
18	302.0
19	497.0
20	818.0
21	1280.0
22	1825.0
23	2507.0
24	3545.0
25	4545.0
26	5593.0
27	6649.0
28	7701.0
29	8894.0
30	10933.0
31	14147.0
32	18774.0
33	26280.0
34	37943.0
35	46929.0
36	55927.0
37	87754.0
38	109502.0
39	104893.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.776664216433296	21.50761066389372	25.88360793875612	11.832117180916866
2	31.65265252702727	25.038815282284816	25.938469261523565	17.37006292916435
3	32.460691682951754	24.223425426250962	26.821987557595424	16.49389533320186
4	28.993850511859726	25.910142173297235	26.353873460386897	18.74213385445614
5	22.083116696846368	39.882926654355735	20.948419599476487	17.085537049321406
6	25.670975491690122	35.50926008928411	24.12553561503846	14.694228803987306
7	69.9327679868046	20.02527923696147	6.241461534324184	3.8004912419097483
8	84.69458737472435	5.416210983021676	6.560768775660218	3.328432866593757
9	78.91908851318644	7.391577173386881	8.265951915664163	5.423382397762518
10	40.862900478691934	28.504760026534235	16.078311848970007	14.554027645803828
11	30.953260304426557	27.900568334618214	23.33345285691235	17.812718504042884
12	30.154364702296643	24.058303601843054	27.12336626207935	18.663965433780948
13	26.020940531043262	24.68293382577048	28.43268730838876	20.863438334797497
14	21.082883625867293	27.817200638255912	28.36724814887857	22.732667586998225
15	20.269286623518653	27.208885382863908	32.03345464976603	20.488373343851407
16	24.11119278555677	25.332484715922334	30.29115943847823	20.26516306004267
17	24.30320741524284	25.579360668375855	27.616221740143786	22.501210176237517
18	24.752675834125178	26.33379349911254	28.08075012998189	20.832780536780394
19	26.018609821252486	27.89393477598293	25.661114796421465	20.426340606343114
20	27.63809455510336	26.319271384262333	26.706886351004893	19.335747709629416
21	26.724814887857	26.480090359825738	26.758341251770446	20.03675350054682
22	25.688007601699624	24.50633773777722	27.144521935564836	22.661132724958318
23	24.438747153844776	26.131021747315202	27.730964375997274	21.69926672284275
24	23.526543198809545	25.457984473887084	28.92608064255876	22.08939168474461
25	24.168564103483515	27.20780967065278	26.288972156982272	22.334654068881438
26	22.971117127131254	28.289617584308946	26.410706922208078	22.32855836635172
27	22.842390232533123	28.074654427452177	27.611381035193716	21.471574304820983
28	22.055686035462646	28.748229556985855	27.652258099216525	21.543826308334975
29	22.737329006579774	27.637915269734837	27.075855639421263	22.548900084264123
30	23.59072736074009	26.525090987324525	27.872779102497447	22.011402549437943
31	24.447711422270828	27.594348925184214	26.183552360291877	21.774387292253078
32	24.639188195851336	27.922799720314824	26.06898900980691	21.36902307402693
33	24.576079746131917	27.61048460835111	25.19963425784822	22.61380138766875
34	22.727468311311114	28.121627194004695	27.503451243344028	21.647453251340156
35	22.983129246822166	27.306775194076412	26.546246660810013	23.163848898291413
36	23.123151119637125	29.5295551930007	25.778725998171286	21.568567689190886
37	23.736307079979202	28.84253366082794	25.583125661114796	21.83803359807806
38	23.187335281567673	27.671083062911233	25.891317209602526	23.25026444591857
39	22.988328522509278	27.302113774494863	26.39457123904118	23.314986463954675
40	23.62891514423508	26.58138659304014	27.667138784803775	22.122559477921005
41	20.72413360345662	27.94754110117073	27.958298223281997	23.370027072090647
42	22.003693278591534	27.964393925811713	27.090557039639997	22.941355755956756
43	23.16922745934704	26.62835935959266	27.46042275489897	22.741990426161323
44	22.724241174677733	27.409147139501943	27.135557667138784	22.731054018681533
45	22.801333883141798	26.8447567993976	25.76330745647848	24.590601860982126
46	21.98988830521541	28.808290155440414	25.793248113021495	23.40857342632268
47	22.441508148519997	27.75875360811804	27.948078957276294	21.851659286085663
48	22.15106585151586	26.865733187514568	27.655843806586944	23.32735715438263
49	21.976621187944854	27.33671585061943	27.073524929630494	23.613138031805224
50	20.726464313247394	27.676103053229827	27.490901267547557	24.106531365975222
51	21.04935726195385	26.691288523943562	27.031751438765085	25.227602775337505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	251.0
1	611.0
2	971.0
3	1909.5
4	2848.0
5	2240.5
6	1633.0
7	1360.0
8	1087.0
9	1097.5
10	1108.0
11	1135.0
12	1162.0
13	1158.5
14	1155.0
15	1184.0
16	1213.0
17	1200.0
18	1187.0
19	1389.5
20	1592.0
21	1912.5
22	2233.0
23	2608.5
24	2984.0
25	3644.5
26	5043.5
27	5782.0
28	7068.5
29	8355.0
30	9830.5
31	11306.0
32	13090.5
33	14875.0
34	17177.5
35	19480.0
36	20519.0
37	21558.0
38	23297.5
39	25037.0
40	26203.0
41	27369.0
42	28984.5
43	30600.0
44	32666.5
45	34733.0
46	36631.0
47	38529.0
48	39345.0
49	40161.0
50	39962.5
51	39764.0
52	37247.5
53	34731.0
54	33037.5
55	31344.0
56	30245.5
57	29147.0
58	28313.5
59	27480.0
60	27144.0
61	26808.0
62	23811.5
63	20815.0
64	18032.5
65	15250.0
66	12747.0
67	10244.0
68	8747.0
69	7250.0
70	6172.5
71	5095.0
72	4187.5
73	3280.0
74	2716.0
75	1726.5
76	1301.0
77	1023.0
78	745.0
79	551.0
80	357.0
81	282.5
82	208.0
83	163.0
84	118.0
85	97.5
86	77.0
87	59.0
88	41.0
89	31.0
90	21.0
91	19.5
92	18.0
93	11.5
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	557770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.563480372017914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.90084935591499	24.16016634700549
2	8.896129332545657	6.149623831447492
3	4.585464541597952	4.754688410403149
4	3.120127076667279	4.313698038903645
5	2.1918087516531752	3.7878269383490806
6	1.661614008169881	3.445869789434982
7	1.2039015582852313	2.9127719515743355
8	0.9719185136480383	2.687430917573984
9	0.7469547689353786	2.323562084539482
>10	6.667395768330808	43.59348962734485
>50	0.04358178629897291	0.9495247244337957
>100	0.009229084157375264	0.6087378552471449
>500	5.12726897631959E-4	0.11767159705505217
>1k	5.12726897631959E-4	0.1949378866875864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.19721390537318248	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	664	0.11904548469799378	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7928536852107499E-4	0.0	0.0	0.028685658963372	0.0
2	1.7928536852107499E-4	0.0	0.0	0.13177474586299012	0.0
3	1.7928536852107499E-4	0.0	0.0	0.1957796224250139	0.0
4	1.7928536852107499E-4	0.0	0.0	0.2949244312171684	0.0
5	1.7928536852107499E-4	0.0	0.0	0.5943309966473637	0.0
6	1.7928536852107499E-4	0.0	0.0	0.8429998027860947	0.0
7	1.7928536852107499E-4	0.0	0.0	1.0561701059576527	0.0
8	1.7928536852107499E-4	0.0	0.0	1.503845671154777	0.0
9	1.7928536852107499E-4	0.0	0.0	1.7173745450633773	0.0
10	1.7928536852107499E-4	0.0	0.0	2.1914050594331	0.0
11	1.7928536852107499E-4	0.0	0.0	2.6527063126378256	0.0
12	1.7928536852107499E-4	0.0	0.0	3.0562776771787656	0.0
13	1.7928536852107499E-4	0.0	0.0	3.208490955053158	0.0
14	1.7928536852107499E-4	0.0	0.0	3.284866522043136	0.0
15	1.7928536852107499E-4	0.0	0.0	3.4098284239023253	0.0
16	1.7928536852107499E-4	0.0	0.0	3.6617243666744357	0.0
17	1.7928536852107499E-4	0.0	0.0	3.9392581171450596	0.0
18	1.7928536852107499E-4	0.0	0.0	4.242967531419761	0.0
19	1.7928536852107499E-4	0.0	0.0	4.4238664682575255	0.0
20	1.7928536852107499E-4	0.0	0.0	4.6219768004733135	0.0
21	1.7928536852107499E-4	0.0	0.0	4.87333488713986	0.0
22	1.7928536852107499E-4	0.0	0.0	5.1467450741344996	0.0
23	1.7928536852107499E-4	0.0	0.0	5.419258834286534	0.0
24	1.7928536852107499E-4	0.0	0.0	5.636014844828513	0.0
25	1.7928536852107499E-4	0.0	0.0	5.835918030729512	0.0
26	1.7928536852107499E-4	0.0	0.0	6.015920540724672	0.0
27	1.7928536852107499E-4	0.0	0.0	6.216899438836797	0.0
28	1.7928536852107499E-4	0.0	0.0	6.411065492945121	0.0
29	1.7928536852107499E-4	0.0	0.0	6.626566505907453	0.0
30	1.7928536852107499E-4	0.0	0.0	6.880793158470337	0.0
31	1.7928536852107499E-4	0.0	0.0	7.093604890904853	0.0
32	1.7928536852107499E-4	0.0	0.0	7.30659590870789	0.0
33	1.7928536852107499E-4	0.0	0.0	7.516539075246069	0.0
34	1.7928536852107499E-4	0.0	0.0	7.757319325169873	0.0
35	1.7928536852107499E-4	0.0	0.0	8.01011169478459	0.0
36	1.7928536852107499E-4	0.0	0.0	8.249099091023181	0.0
37	3.5857073704214997E-4	0.0	0.0	8.487727916524733	0.0
38	3.5857073704214997E-4	0.0	0.0	8.741954569087616	0.0
39	3.5857073704214997E-4	0.0	0.0	9.069508937375621	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0301595E-4	45.000004	1
CTATGCG	20	7.0301595E-4	45.000004	1
AAGTACG	25	3.8882135E-5	45.000004	1
ACGGGCC	65	0.0	45.000004	5
ATACGTG	20	7.0301595E-4	45.000004	11
TAATGCG	20	7.0301595E-4	45.000004	1
CCGCATG	20	7.0301595E-4	45.000004	1
GTACGAT	20	7.0301595E-4	45.000004	13
CTTACGG	25	3.8882135E-5	45.000004	2
TAGCACG	30	2.1634842E-6	44.999996	1
TACGAAT	165	0.0	42.272728	12
CGAATAT	170	0.0	41.02941	14
CGGGTAG	45	1.9252184E-8	40.0	6
ACCGGGT	45	1.9252184E-8	40.0	4
TATGGGC	165	0.0	39.545452	4
AACGGGC	115	0.0	39.130432	4
CTACGAA	180	0.0	38.75	11
CTAGACG	35	6.242506E-6	38.571426	1
GCGATAT	35	6.242506E-6	38.571426	9
GGCCTAT	65	9.094947E-12	38.076927	8
>>END_MODULE