##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936285.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279396
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.667915789775087	31.0	31.0	34.0	30.0	34.0
2	31.787541697089434	31.0	31.0	34.0	30.0	34.0
3	31.759216309467565	31.0	31.0	34.0	30.0	34.0
4	35.51721928731979	37.0	35.0	37.0	33.0	37.0
5	30.97485289696345	35.0	35.0	37.0	0.0	37.0
6	33.200532577417	36.0	35.0	37.0	17.0	37.0
7	29.63621168520666	35.0	32.0	37.0	0.0	37.0
8	32.51663230683331	35.0	33.0	37.0	17.0	37.0
9	36.214688112929316	39.0	35.0	39.0	32.0	39.0
10	36.60388480865868	38.0	35.0	39.0	32.0	39.0
11	36.72310627210125	38.0	35.0	39.0	33.0	39.0
12	36.66935818694613	39.0	35.0	39.0	33.0	39.0
13	36.35542026371172	39.0	35.0	39.0	32.0	39.0
14	37.49143151655714	39.0	36.0	41.0	32.0	41.0
15	37.58967916505605	39.0	36.0	41.0	33.0	41.0
16	37.70324557259231	39.0	36.0	41.0	33.0	41.0
17	37.64449025755558	39.0	36.0	41.0	33.0	41.0
18	37.55040158055233	39.0	36.0	41.0	33.0	41.0
19	37.46718277999685	39.0	36.0	41.0	32.0	41.0
20	37.435253189022035	39.0	36.0	41.0	32.0	41.0
21	37.32949290612607	39.0	35.0	41.0	32.0	41.0
22	37.33911365946542	39.0	35.0	41.0	32.0	41.0
23	37.317778350441664	39.0	35.0	40.0	32.0	41.0
24	37.24504287820871	39.0	35.0	41.0	32.0	41.0
25	37.118870706810405	39.0	35.0	40.0	32.0	41.0
26	36.93273704705865	39.0	35.0	40.0	31.0	41.0
27	36.89993772280204	39.0	35.0	40.0	31.0	41.0
28	36.75195063637275	39.0	35.0	40.0	31.0	41.0
29	36.75341808758894	39.0	35.0	40.0	31.0	41.0
30	36.64447952010766	39.0	35.0	40.0	31.0	41.0
31	36.48173918023164	39.0	35.0	40.0	30.0	41.0
32	36.373140631934604	38.0	35.0	40.0	30.0	41.0
33	36.21593365688843	38.0	35.0	40.0	30.0	41.0
34	36.10418187805122	38.0	35.0	40.0	30.0	41.0
35	35.99064052456012	38.0	35.0	40.0	29.0	41.0
36	35.807101748056525	38.0	35.0	40.0	29.0	41.0
37	35.74546163867772	38.0	35.0	40.0	29.0	41.0
38	35.56686208821887	38.0	34.0	40.0	27.0	41.0
39	35.6232336898166	38.0	35.0	40.0	28.0	41.0
40	35.43488453664333	38.0	34.0	40.0	27.0	41.0
41	35.301321421924435	38.0	34.0	40.0	26.0	41.0
42	35.27222293805208	38.0	34.0	40.0	26.0	41.0
43	35.096300591275465	38.0	34.0	40.0	26.0	41.0
44	34.91405030852267	38.0	34.0	40.0	25.0	41.0
45	34.799231198728684	38.0	34.0	40.0	24.0	41.0
46	34.609640080745606	37.0	33.0	40.0	24.0	41.0
47	34.36810476885854	37.0	33.0	40.0	23.0	41.0
48	34.23416226431302	37.0	33.0	40.0	23.0	41.0
49	34.00419476298873	37.0	33.0	40.0	22.0	41.0
50	33.79918109063838	37.0	32.0	40.0	22.0	41.0
51	31.354389468711076	35.0	28.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	12.0
10	11.0
11	15.0
12	9.0
13	6.0
14	7.0
15	9.0
16	39.0
17	77.0
18	131.0
19	270.0
20	458.0
21	695.0
22	1095.0
23	1525.0
24	1959.0
25	2829.0
26	3353.0
27	4129.0
28	4956.0
29	6012.0
30	7418.0
31	9488.0
32	11972.0
33	16483.0
34	23060.0
35	26543.0
36	28952.0
37	41122.0
38	46906.0
39	39846.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58689458689459	19.816675972454867	24.40263998052943	11.193789460121119
2	29.629271714698852	26.63280791421495	25.09520537158728	18.642714999498917
3	30.89271142034961	27.608484015519192	25.21689644805223	16.28190811607897
4	27.659665850620623	24.1961230654698	28.358315795501728	19.78589528840785
5	23.93627682572406	36.56136809403141	21.531804320749046	17.97055075949548
6	25.192916147690013	35.55813254305716	24.982462168391816	14.266489140861
7	66.32056292860314	18.59010150467437	10.064925768443356	5.0244097982791445
8	76.06372317427595	7.537688442211055	10.158341565376741	6.240246818136265
9	69.96521066872826	8.058812581425647	12.201677905195494	9.774298844650604
10	40.468367478417726	26.046185342667755	19.069349596987788	14.41609758192673
11	30.487909633638278	25.02362238543143	25.151755930650406	19.33671205027989
12	27.36474394765852	22.631318988102908	30.269223610932155	19.73471345330642
13	23.335337656945697	25.730862288651235	31.9693195321336	18.96448052226947
14	18.119801285630434	30.247390800154616	29.71910836232444	21.913699551890506
15	16.256854070924422	25.898008561325142	39.18058955747398	18.664547810276453
16	19.78625320333863	26.81319703932769	31.446047903334335	21.954501853999343
17	20.52821085484402	24.910879182235966	29.159329410585695	25.40158055233432
18	22.865753267763317	25.26521496370743	30.65434007645063	21.214691692078627
19	22.963464043866054	26.83825108448224	27.830749187533105	22.367535684118597
20	24.11308680152901	27.286718492748648	29.778164325903017	18.822030379819324
21	23.235479391258284	27.994316310899226	29.250955632865182	19.519248664977308
22	21.54397342839554	24.058325817119787	27.88407851221922	26.513622242265463
23	20.028203696545404	27.636043465189193	29.465346676401953	22.87040616186345
24	22.77484287534539	23.268407564889976	29.679737719938725	24.27701183982591
25	18.955532648999988	29.189752179701927	27.92559664418961	23.929118527108475
26	20.13199902647139	27.435253189022035	28.57199100917694	23.86075677532964
27	22.53432404186173	28.390170224341077	26.434880957494023	22.640624776303166
28	17.370327420578676	26.332875202221935	32.34155106014403	23.955246317055362
29	20.411888502340762	24.045082964680955	29.205500436656216	26.337528096322067
30	22.388652665034574	24.81316840613323	29.664705292845994	23.1334736359862
31	22.440550329997567	25.28668985955418	27.73196466663803	24.540795143810218
32	23.04793196752996	26.168234334063477	29.090251828945295	21.69358186946127
33	21.775544388609717	25.14889261120417	26.486062792595455	26.58950020759066
34	19.54143939068562	24.523257312202034	29.347950579106357	26.587352718005985
35	20.313819811307248	24.293833841572535	29.961416770461994	25.43092957665822
36	21.197869690332002	27.050852553365118	28.462826955289266	23.288450801013617
37	20.352474623831405	25.9470429068419	31.60746753711578	22.09301493221091
38	19.123752666466235	26.92128734842303	29.17937264670933	24.775587338401408
39	20.46163867771908	24.98031467880714	29.277441337742843	25.280605305730937
40	22.234033414937937	23.057237755730217	30.467866397514637	24.240862431817206
41	19.186029864421826	25.816403957107475	28.194748672135606	26.802817506335092
42	21.00745894715744	23.75481395581898	31.269237927529385	23.968489169494195
43	23.19539291901101	24.34000486764306	27.975346819567925	24.489255393778006
44	20.258700911967242	24.342152357227732	29.080230210883478	26.318916519921544
45	19.836719208578504	23.530043379289612	27.993600481037667	28.63963693109422
46	22.192157368036767	24.874729774227262	28.18293747941989	24.75017537831608
47	18.67671691792295	24.36935389196696	32.80540881043393	24.148520379676157
48	20.408309353032973	24.074789903935635	30.29105642170969	25.22584432132171
49	20.67495597646351	21.545763003049434	33.122521439104354	24.656759581382698
50	19.2848143853169	23.396899025039726	31.35728500050108	25.961001589142292
51	19.246875402654297	22.418001689358473	28.528325387621873	29.806797520365357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	344.0
2	457.0
3	598.0
4	739.0
5	630.0
6	521.0
7	473.5
8	426.0
9	453.0
10	480.0
11	536.0
12	592.0
13	638.5
14	685.0
15	711.0
16	737.0
17	722.5
18	708.0
19	767.5
20	827.0
21	957.5
22	1088.0
23	1227.0
24	1366.0
25	1624.0
26	2030.0
27	2178.0
28	2865.5
29	3553.0
30	3949.5
31	4346.0
32	5281.5
33	6217.0
34	7264.0
35	8311.0
36	8189.0
37	8067.0
38	9224.0
39	10381.0
40	11684.0
41	12987.0
42	15448.0
43	17909.0
44	20900.5
45	23892.0
46	28500.0
47	33108.0
48	30900.5
49	28693.0
50	27123.5
51	25554.0
52	22299.5
53	19045.0
54	16839.5
55	14634.0
56	13311.0
57	11988.0
58	10832.0
59	9676.0
60	8923.0
61	8170.0
62	7276.5
63	6383.0
64	5589.5
65	4796.0
66	4038.0
67	3280.0
68	2690.0
69	2100.0
70	1705.5
71	1311.0
72	1123.0
73	935.0
74	719.0
75	373.0
76	243.0
77	208.5
78	174.0
79	131.5
80	89.0
81	66.0
82	43.0
83	39.5
84	36.0
85	34.0
86	32.0
87	23.5
88	15.0
89	9.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.95486026132753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.70502286903057	26.835992627680227
2	11.399616191283053	8.653416791458302
3	4.777939795809768	5.440381118609867
4	2.7839657051832813	4.2266011725023835
5	1.7903957642530388	3.3977110522348397
6	1.2043867030949458	2.7427397409941783
7	0.9772323525987385	2.596350217001343
8	0.7232085294026457	2.1959422938622084
9	0.5608348291004339	1.9157766811373225
>10	4.993720319205495	36.10068264268701
>50	0.05478847329872188	1.3649008620407113
>100	0.023907697439442273	2.049619826349778
>500	0.0019923081199535227	0.48470994602110584
>1k	0.002988462179930284	1.9951750274207296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	2022	0.7237039900356483	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	1674	0.5991495941244684	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	1581	0.565863505561998	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	766	0.27416283697690735	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	516	0.18468410428209422	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	388	0.1388709931423499	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC	353	0.1263439705650761	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	343	0.12276482125728357	TruSeq Adapter, Index 16 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	318	0.11381694798780226	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	317	0.113459033057023	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	313	0.112027373333906	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	311	0.1113115434723475	No Hit
GAATCTATCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	296	0.1059428195106587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20544317026729086	0.0
2	0.0	0.0	0.0	1.039384958982949	0.0
3	0.0	0.0	0.0	1.358645077238042	0.0
4	0.0	0.0	0.0	1.8965912181992584	0.0
5	0.0	0.0	0.0	3.854385889561769	0.0
6	0.0	0.0	0.0	4.676516485561712	0.0
7	0.0	0.0	0.0	5.699795272659594	0.0
8	0.0	0.0	0.0	7.227018282294664	0.0
9	0.0	0.0	0.0	7.739194548239774	0.0
10	0.0	0.0	0.0	9.894916176323212	0.0
11	0.0	0.0	0.0	11.403527609557761	0.0
12	0.0	0.0	0.0	13.405703732336898	0.0
13	0.0	0.0	0.0	13.991968388953314	0.0
14	0.0	0.0	0.0	14.260404587037753	0.0
15	0.0	0.0	0.0	14.801214047445203	0.0
16	0.0	0.0	0.0	15.709244226832167	0.0
17	0.0	0.0	0.0	16.65127632464316	0.0
18	0.0	0.0	0.0	17.83776432017638	0.0
19	0.0	0.0	0.0	18.483084940371374	0.0
20	0.0	0.0	0.0	19.075792065741815	0.0
21	0.0	0.0	0.0	19.85389912525591	0.0
22	0.0	0.0	0.0	20.626637460808315	0.0
23	0.0	0.0	0.0	21.401881200876176	0.0
24	0.0	0.0	0.0	21.95092270469155	0.0
25	0.0	0.0	0.0	22.470257269252244	0.0
26	0.0	0.0	0.0	22.939125828573065	0.0
27	0.0	0.0	0.0	23.39618319517817	0.0
28	0.0	0.0	0.0	23.877578777076266	0.0
29	0.0	0.0	0.0	24.393692107259948	0.0
30	0.0	0.0	0.0	25.05619264413234	0.0
31	0.0	0.0	0.0	25.59020172085499	0.0
32	0.0	0.0	0.0	26.122063307992956	0.0
33	0.0	0.0	0.0	26.611690933298974	0.0
34	0.0	0.0	0.0	27.099528983951096	0.0
35	0.0	0.0	0.0	27.65608670131283	0.0
36	0.0	0.0	0.0	28.171484201634957	0.0
37	0.0	0.0	0.0	28.696903320018897	0.0
38	0.0	0.0	0.0	29.220532863748943	0.0
39	0.0	0.0	0.0	29.80142879640367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	20	7.024109E-4	45.000004	39
AAGTACG	20	7.024109E-4	45.000004	1
TCGTTAT	20	7.024109E-4	45.000004	21
ATAGGCG	20	7.024109E-4	45.000004	14
CTCCGAA	20	7.024109E-4	45.000004	36
TTTACGG	20	7.024109E-4	45.000004	2
CACGGAC	20	7.024109E-4	45.000004	15
TTAGACT	20	7.024109E-4	45.000004	27
GCACGGT	20	7.024109E-4	45.000004	3
CGTTATG	20	7.024109E-4	45.000004	22
TTGAGCG	20	7.024109E-4	45.000004	1
TGTTGCG	20	7.024109E-4	45.000004	13
TGGCACG	20	7.024109E-4	45.000004	1
CCGTATG	20	7.024109E-4	45.000004	39
GCCGAAT	20	7.024109E-4	45.000004	22
CGCTCAT	20	7.024109E-4	45.000004	11
AACGCGT	20	7.024109E-4	45.000004	31
CCGAGAA	20	7.024109E-4	45.000004	26
ACTCGTC	20	7.024109E-4	45.000004	10
TTCGAGT	20	7.024109E-4	45.000004	17
>>END_MODULE