##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	820364
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720794671633566	31.0	31.0	34.0	30.0	34.0
2	31.787477266189153	33.0	31.0	34.0	30.0	34.0
3	31.2012326235671	31.0	31.0	34.0	28.0	34.0
4	35.249867132151095	37.0	35.0	37.0	32.0	37.0
5	30.896559332198876	35.0	35.0	37.0	0.0	37.0
6	33.221440726311734	37.0	35.0	37.0	17.0	37.0
7	29.675285604926593	35.0	32.0	37.0	0.0	37.0
8	32.62026734473965	35.0	33.0	37.0	17.0	37.0
9	36.43174005685281	39.0	35.0	39.0	32.0	39.0
10	36.903897538165985	38.0	37.0	39.0	34.0	39.0
11	37.052497427971	39.0	37.0	39.0	34.0	39.0
12	37.08363970140084	39.0	37.0	39.0	34.0	39.0
13	36.94944683091896	39.0	37.0	39.0	33.0	39.0
14	38.2945692399959	40.0	38.0	41.0	34.0	41.0
15	38.334709958018635	40.0	38.0	41.0	34.0	41.0
16	38.36055336411642	40.0	38.0	41.0	34.0	41.0
17	38.29464237826136	40.0	37.0	41.0	33.0	41.0
18	38.10611996625888	40.0	37.0	41.0	33.0	41.0
19	37.92178593892467	40.0	37.0	41.0	33.0	41.0
20	37.71717554646474	39.0	35.0	41.0	33.0	41.0
21	37.623475676650855	39.0	35.0	41.0	33.0	41.0
22	37.57058573998859	39.0	35.0	41.0	33.0	41.0
23	37.50703346319439	39.0	35.0	41.0	33.0	41.0
24	37.423803336080084	39.0	35.0	41.0	33.0	41.0
25	37.280644933224764	39.0	35.0	41.0	33.0	41.0
26	37.106875728335226	39.0	35.0	41.0	32.0	41.0
27	37.07182543358802	39.0	35.0	41.0	32.0	41.0
28	36.899637234203354	39.0	35.0	41.0	32.0	41.0
29	36.829856015134744	39.0	35.0	41.0	31.0	41.0
30	36.64242336328751	39.0	35.0	40.0	31.0	41.0
31	36.47464296336748	39.0	35.0	40.0	30.0	41.0
32	36.24023847950422	39.0	35.0	40.0	30.0	41.0
33	35.83407974996465	38.0	35.0	40.0	27.0	41.0
34	35.45033424187312	38.0	35.0	40.0	25.0	41.0
35	35.119453072050945	38.0	34.0	40.0	23.0	41.0
36	34.848507491796326	38.0	34.0	40.0	22.0	41.0
37	34.676521641612744	38.0	34.0	40.0	21.0	41.0
38	34.480085669288265	38.0	34.0	40.0	20.0	41.0
39	34.3901792375092	38.0	34.0	40.0	19.0	41.0
40	34.19674437201047	38.0	33.0	40.0	18.0	41.0
41	34.00272805730139	38.0	33.0	40.0	18.0	41.0
42	33.938255457333575	38.0	33.0	40.0	17.0	41.0
43	33.75118727784252	38.0	33.0	40.0	15.0	41.0
44	33.59358406756025	38.0	33.0	40.0	15.0	41.0
45	33.50056560258617	38.0	33.0	40.0	15.0	41.0
46	33.314784656567085	37.0	32.0	40.0	15.0	41.0
47	33.140720460673556	37.0	32.0	40.0	13.0	41.0
48	33.01756903033288	37.0	32.0	40.0	13.0	41.0
49	32.86855347138587	37.0	31.0	40.0	13.0	41.0
50	32.72256583662862	37.0	31.0	40.0	12.0	41.0
51	30.89021580664193	35.0	27.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	17.0
9	41.0
10	37.0
11	45.0
12	40.0
13	25.0
14	33.0
15	60.0
16	121.0
17	230.0
18	511.0
19	882.0
20	1632.0
21	2794.0
22	4231.0
23	6616.0
24	9923.0
25	15187.0
26	19279.0
27	20013.0
28	19767.0
29	19255.0
30	20429.0
31	23849.0
32	28965.0
33	38574.0
34	53496.0
35	64382.0
36	75057.0
37	116336.0
38	143269.0
39	135249.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.20503581337065	19.149182557986457	22.826574544958092	18.8192070836848
2	36.65884899873715	24.64747356051704	22.999302748536017	15.6943746922098
3	30.753421651852104	24.54069169295581	30.067872310340288	14.638014344851797
4	26.13595915959257	23.4185069066902	33.47489650935439	16.970637424362845
5	21.11672379577846	37.36122014130313	26.157534947901173	15.364521115017235
6	23.430940411817193	33.67736760754982	29.751427415147425	13.140264565485566
7	63.159524308721494	18.529945243818595	14.451755562165088	3.8587748852948205
8	75.29986688835689	6.133374940879902	15.209346095148007	3.3574120756152146
9	68.91562769697353	8.129927690634888	16.60433661155292	6.3501080008386515
10	34.89182850539517	30.984050982246902	22.13858726126451	11.985533251093416
11	27.603234661686766	25.272196244593864	31.403742728837443	15.72082636488193
12	27.01556869877274	21.86285600050709	33.971383434670464	17.150191866049706
13	23.31123745069262	21.860539955434415	35.65709855625064	19.171124037622324
14	18.96463033482698	26.79200940070505	33.57741197809753	20.66594828637044
15	18.81676914150304	25.962621470469205	37.45995679966454	17.760652588363214
16	21.90929879906968	25.89801600265248	34.51492264409457	17.677762554183264
17	22.09702034706545	25.30742450912034	33.0422836692005	19.55327147461371
18	21.565792745659245	26.68681219556197	32.89137504814936	18.85602001062943
19	23.069027894934443	26.544192577928822	31.557333086288526	18.82944644084821
20	24.56324265813712	25.887410954161812	32.15572599480231	17.39362039289876
21	24.329322105796937	26.033443690849428	32.333939568289196	17.303294635064432
22	22.860218147066426	23.828446884553685	32.8137265896602	20.49760837871969
23	23.090116094806696	25.33692360951968	33.05752080783652	18.515439487837106
24	22.451009551857467	24.012023930840456	33.22110672823283	20.315859789069243
25	22.28522948349757	26.836501845522232	31.44628481990921	19.43198385107099
26	21.46717798440692	26.927437088902	31.473345978126783	20.132038948564297
27	22.27267408126149	26.929143648429232	31.57634903530628	19.221833235003
28	20.600001950353743	26.703146408179784	32.88857141464033	19.80828022682614
29	22.56217971534587	25.789039987127666	31.8706086566451	19.778171640881364
30	21.523860140132918	26.41498164229537	32.17925213685632	19.88190608071539
31	22.333135047369215	25.469913355534864	30.922614839266473	21.274336757829452
32	23.16471712556865	26.28455173557104	31.017328893027972	19.533402245832338
33	22.071421954156936	27.586169066414424	30.405405405405407	19.937003574023237
34	21.986825360449753	26.805783774032015	32.07588338834956	19.13150747716867
35	21.79459361941772	26.430462575149566	31.363150991511084	20.411792813921625
36	22.307292860242526	28.339005612142902	29.784095840383053	19.569605687231522
37	22.07715111828408	28.552203655938097	30.352136368733877	19.018508857043948
38	21.385263127099677	28.11739666782063	29.032843957072714	21.46449624800698
39	23.29795066580201	26.136812439356188	29.584184581478468	20.981052313363335
40	22.867897664938976	26.378290612459836	31.497725399944414	19.25608632265677
41	21.08893125490636	27.82082100140913	30.72575100808909	20.36449673559542
42	21.724380884582942	27.515834434470555	30.38443910264224	20.375345578304266
43	22.71516058725151	26.910371493629658	29.280538882739858	21.093929036378974
44	22.143585042737126	27.441964786363126	28.74029089526113	21.67415927563862
45	21.494970525279022	26.034296970613045	29.149865181797345	23.320867322310583
46	22.06934970330244	28.003422870823197	28.47041069573994	21.456816730134427
47	22.554500197473317	26.207878453954585	30.742816603361433	20.49480474521066
48	21.796543973163132	25.838164522090196	30.235846526663774	22.129444978082898
49	21.014695915471666	25.810128186999915	30.902867507594188	22.272308389934224
50	19.841314343389033	26.406083153331934	31.093636483317162	22.65896601996187
51	20.732382210823488	25.61509281245886	30.164536717847202	23.487988258870452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	375.0
1	850.0
2	1325.0
3	13101.0
4	24877.0
5	19237.0
6	13597.0
7	10837.5
8	8078.0
9	7618.0
10	7158.0
11	6820.5
12	6483.0
13	6216.0
14	5949.0
15	5669.0
16	5389.0
17	5159.5
18	4930.0
19	4883.0
20	4836.0
21	4848.0
22	4860.0
23	5014.5
24	5169.0
25	5870.0
26	7512.0
27	8453.0
28	10565.0
29	12677.0
30	14780.5
31	16884.0
32	18703.0
33	20522.0
34	21845.5
35	23169.0
36	24892.0
37	26615.0
38	29125.0
39	31635.0
40	34570.5
41	37506.0
42	40038.0
43	42570.0
44	47799.0
45	53028.0
46	59330.5
47	65633.0
48	61700.0
49	57767.0
50	55115.0
51	52463.0
52	48772.5
53	45082.0
54	42537.5
55	39993.0
56	38607.5
57	37222.0
58	36599.0
59	35976.0
60	35225.0
61	34474.0
62	30703.0
63	26932.0
64	22977.5
65	19023.0
66	15670.0
67	12317.0
68	9932.5
69	7548.0
70	6228.0
71	4908.0
72	4063.0
73	3218.0
74	2618.5
75	1611.5
76	1204.0
77	1032.0
78	860.0
79	679.5
80	499.0
81	361.0
82	223.0
83	184.5
84	146.0
85	120.5
86	95.0
87	70.0
88	45.0
89	35.5
90	26.0
91	22.0
92	18.0
93	14.0
94	10.0
95	8.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	820364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.57446937222957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1671848979028	30.092941720644443
2	8.52907279054044	6.921252054265993
3	4.263645514365408	5.189854630099898
4	2.67143536214271	4.335682891246016
5	1.8345164269226604	3.7217265288512755
6	1.27643414480111	3.107438286834041
7	1.0217514272669717	2.9019915394172915
8	0.802856569305483	2.6060383425262907
9	0.65673137277794	2.3981874273504724
>10	4.703287591019007	31.91844980718682
>50	0.043241356903791044	1.1410390807100192
>100	0.024665777859136158	1.8399908929151993
>500	0.0030451577603455514	0.7478824116793663
>1k	0.0018270946562073307	1.6823338703826616
>5k	0.0	0.0
>10k+	3.0451577603455513E-4	1.3951905158902038
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11292	1.3764621558235124	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3434	0.4185946726087444	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	2502	0.30498656693857845	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2274	0.2771940260664778	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	2068	0.2520832215943167	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	1704	0.2077126738862261	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1634	0.19917987625005487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.875884363526435E-4	0.0	0.0	0.0442486505990024	0.0
2	4.875884363526435E-4	0.0	0.0	0.22733810844942	0.0
3	4.875884363526435E-4	0.0	0.0	0.3459439955922005	0.0
4	4.875884363526435E-4	0.0	0.0	0.5886411397867288	0.0
5	4.875884363526435E-4	0.0	0.0	1.4759301968394518	0.0
6	4.875884363526435E-4	0.0	0.0	2.060304937808095	0.0
7	4.875884363526435E-4	0.0	0.0	2.499865913180003	0.0
8	4.875884363526435E-4	0.0	0.0	3.130317761383971	0.0
9	4.875884363526435E-4	0.0	0.0	3.477602625176141	0.0
10	4.875884363526435E-4	0.0	0.0	4.4068242877551915	0.0
11	4.875884363526435E-4	0.0	0.0	4.827003622782082	0.0
12	4.875884363526435E-4	0.0	0.0	5.530471839329858	0.0
13	4.875884363526435E-4	0.0	0.0	5.755493902706603	0.0
14	4.875884363526435E-4	0.0	0.0	5.87507496672209	0.0
15	4.875884363526435E-4	0.0	0.0	6.030006192373142	0.0
16	4.875884363526435E-4	0.0	0.0	6.239913014222955	0.0
17	4.875884363526435E-4	0.0	0.0	6.475783920308546	0.0
18	4.875884363526435E-4	0.0	0.0	6.7921069183923235	0.0
19	4.875884363526435E-4	0.0	0.0	6.963372356661189	0.0
20	4.875884363526435E-4	0.0	0.0	7.162430335802156	0.0
21	4.875884363526435E-4	0.0	0.0	7.382820309033551	0.0
22	4.875884363526435E-4	0.0	0.0	7.626614527209873	0.0
23	6.094855454408043E-4	0.0	0.0	7.872237202022517	0.0
24	6.094855454408043E-4	0.0	0.0	8.068735341872632	0.0
25	6.094855454408043E-4	0.0	0.0	8.256700684086576	0.0
26	6.094855454408043E-4	0.0	0.0	8.439668244827905	0.0
27	7.313826545289651E-4	0.0	0.0	8.617638024096621	0.0
28	7.313826545289651E-4	0.0	0.0	8.816817900346676	0.0
29	7.313826545289651E-4	0.0	0.0	9.016729159251259	0.0
30	7.313826545289651E-4	0.0	0.0	9.268202895300135	0.0
31	7.313826545289651E-4	0.0	0.0	9.497125666167701	0.0
32	7.313826545289651E-4	0.0	0.0	9.724219980398944	0.0
33	7.313826545289651E-4	0.0	0.0	9.94521943917578	0.0
34	7.313826545289651E-4	0.0	0.0	10.16987581122526	0.0
35	7.313826545289651E-4	0.0	0.0	10.39721391967468	0.0
36	7.313826545289651E-4	0.0	0.0	10.63978916676012	0.0
37	7.313826545289651E-4	0.0	0.0	10.8850461502455	0.0
38	7.313826545289651E-4	0.0	0.0	11.16077741100292	0.0
39	7.313826545289651E-4	0.0	0.0	11.43992179081481	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCGA	30	2.1647393E-6	45.000004	27
CGAATAT	70	0.0	45.0	14
CGTTATT	1215	0.0	43.14815	1
CGTTTTT	4740	0.0	43.10127	1
CGTTTTA	530	0.0	42.0283	1
CGGTTTT	1250	0.0	41.76	1
ACGGGTA	60	3.6379788E-12	41.250004	5
ATTAGCG	45	1.9272193E-8	40.0	1
CGCACGG	40	3.4566074E-7	39.375	2
CACGACC	35	6.246104E-6	38.571426	27
TACGACG	35	6.246104E-6	38.571426	1
TTAACGG	70	0.0	38.571426	2
GTTATTT	1425	0.0	38.052635	2
TTACGGG	315	0.0	37.857143	3
CTAGACG	30	1.13963695E-4	37.500004	1
TCGTCAC	30	1.13963695E-4	37.500004	27
TATTACG	30	1.13963695E-4	37.500004	1
TACGTGC	30	1.13963695E-4	37.500004	16
AAGGGCG	210	0.0	37.5	5
TTTACGG	85	0.0	37.058823	2
>>END_MODULE