##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413910
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930656422893865	31.0	31.0	34.0	30.0	34.0
2	32.06115822280206	33.0	31.0	34.0	30.0	34.0
3	32.02411635379672	33.0	31.0	34.0	30.0	34.0
4	35.728293590394046	37.0	35.0	37.0	35.0	37.0
5	31.215602425648086	37.0	35.0	37.0	0.0	37.0
6	33.42485806093112	37.0	35.0	37.0	17.0	37.0
7	29.54737020125148	35.0	32.0	37.0	0.0	37.0
8	32.59216013142954	35.0	33.0	37.0	17.0	37.0
9	36.43955690850668	39.0	35.0	39.0	32.0	39.0
10	36.82656616172598	38.0	37.0	39.0	33.0	39.0
11	36.99976081756904	39.0	37.0	39.0	33.0	39.0
12	37.01718247928294	39.0	37.0	39.0	33.0	39.0
13	36.81606387862096	39.0	37.0	39.0	33.0	39.0
14	38.08316783841898	40.0	37.0	41.0	33.0	41.0
15	38.18126162692373	40.0	37.0	41.0	33.0	41.0
16	38.23508492184291	40.0	37.0	41.0	34.0	41.0
17	38.17854364475369	40.0	37.0	41.0	33.0	41.0
18	38.10006281558793	40.0	37.0	41.0	33.0	41.0
19	38.03174361576188	40.0	37.0	41.0	33.0	41.0
20	37.975443937087775	40.0	37.0	41.0	33.0	41.0
21	37.89554009325699	40.0	36.0	41.0	33.0	41.0
22	37.877243845280375	40.0	36.0	41.0	33.0	41.0
23	37.8425478968858	40.0	36.0	41.0	33.0	41.0
24	37.777014326786016	40.0	36.0	41.0	33.0	41.0
25	37.65189050759827	40.0	36.0	41.0	33.0	41.0
26	37.48202990988379	39.0	36.0	41.0	33.0	41.0
27	37.40589258534464	39.0	36.0	41.0	32.0	41.0
28	37.29269164794279	39.0	36.0	41.0	32.0	41.0
29	37.26364185450944	39.0	36.0	41.0	32.0	41.0
30	37.10484404822304	39.0	35.0	41.0	31.0	41.0
31	36.99808895653644	39.0	35.0	41.0	31.0	41.0
32	36.90895122127999	39.0	35.0	41.0	31.0	41.0
33	36.70531758111667	39.0	35.0	41.0	30.0	41.0
34	36.61116184677829	39.0	35.0	41.0	30.0	41.0
35	36.47404025029596	39.0	35.0	41.0	30.0	41.0
36	36.342055036118964	39.0	35.0	41.0	30.0	41.0
37	36.2537532313788	39.0	35.0	40.0	30.0	41.0
38	36.12590418206857	39.0	35.0	40.0	29.0	41.0
39	36.14511850402261	39.0	35.0	40.0	29.0	41.0
40	35.93560677441956	39.0	35.0	40.0	28.0	41.0
41	35.84737020125148	38.0	35.0	40.0	28.0	41.0
42	35.815897175714525	38.0	35.0	40.0	28.0	41.0
43	35.66113406296055	38.0	35.0	40.0	27.0	41.0
44	35.50954555338117	38.0	34.0	40.0	27.0	41.0
45	35.347331545505064	38.0	34.0	40.0	26.0	41.0
46	35.23440844628059	38.0	34.0	40.0	26.0	41.0
47	35.09201517238047	38.0	34.0	40.0	26.0	41.0
48	34.98433234277983	38.0	34.0	40.0	25.0	41.0
49	34.83498103452441	38.0	34.0	40.0	24.0	41.0
50	34.653035684085914	38.0	34.0	40.0	24.0	41.0
51	32.578889130487305	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	7.0
10	18.0
11	19.0
12	12.0
13	11.0
14	20.0
15	29.0
16	30.0
17	88.0
18	178.0
19	362.0
20	549.0
21	946.0
22	1354.0
23	1947.0
24	2634.0
25	3455.0
26	4280.0
27	5130.0
28	5970.0
29	7098.0
30	8926.0
31	11120.0
32	14515.0
33	20302.0
34	29337.0
35	35274.0
36	41046.0
37	63336.0
38	79692.0
39	76208.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.617694667922976	21.095407214128674	24.349254668889373	10.937643449058974
2	30.51532941943901	25.268536638399652	26.03102123650069	18.18511270566065
3	32.658065763088594	24.79137976854872	25.96844724698606	16.582107221376628
4	29.498200091807398	26.002512623517188	26.028363653934427	18.47092363074098
5	21.914909038196708	40.16694450484405	21.158464400473534	16.75968205648571
6	25.97738638834529	36.61158222802058	23.87233939745355	13.53869198618057
7	69.40856707979995	19.621415283515738	6.82346403807591	4.146553598608394
8	83.21374211785171	5.725399241380977	7.084873523229687	3.975985117537629
9	76.31175859486362	8.77364644487932	9.157546326496098	5.757048633760962
10	40.17588364620328	28.89541204609698	16.63767485685294	14.291029450846802
11	30.92918750452997	27.113865333043414	23.63798893479259	18.318958227634027
12	30.61293517914522	23.31787103476601	27.311251238191876	18.757942547896885
13	24.949385132033534	26.314899374260104	28.339252494503636	20.396462999202726
14	19.527191901621123	29.75308641975309	28.093546906332296	22.626174772293496
15	19.431518929235825	27.21678625788215	33.27824889468725	20.07344591819478
16	23.049455195573916	25.515933415476795	31.060858640767318	20.37375274818197
17	21.748206131767773	25.52245657268488	28.454011741682972	24.275325553864366
18	22.66048174723974	27.35812133072407	28.839844410620668	21.141552511415526
19	24.789446981227805	27.66640090841004	26.80582735377256	20.738324756589595
20	27.522891449832088	25.426542001884467	26.854630233625663	20.195936314657775
21	25.506511077287332	27.077142373946028	26.671981831799187	20.744364716967457
22	22.919233649827255	26.239279070329296	26.70194003527337	24.139547244570075
23	23.746225024763838	26.854630233625663	27.607450895122128	21.791693846488368
24	23.217124495663306	25.239544828585924	28.96909956270687	22.5742311130439
25	22.5189050759827	27.30714406513493	26.730448648256868	23.443502210625496
26	21.615568601869974	29.584933922833468	26.59322074847189	22.206276726824672
27	22.78273054528762	28.563455823729793	27.624845981010367	21.028967649972216
28	20.959628904834386	29.12299775313474	28.50595540093257	21.411417941098307
29	23.349520427145997	26.786741078978523	28.0785678045952	21.785170689280278
30	21.94124326544418	28.048368002705903	28.32234060544563	21.68804812640429
31	24.6601918291416	27.50597956077408	25.590828924162256	22.24299968592206
32	25.456017008528427	28.907975163682924	25.020415066077167	20.615592761711483
33	24.0073929115025	29.328114807566863	25.55700514604624	21.107487134884394
34	23.55512067840835	27.716170181923605	25.99478147423353	22.733927665434514
35	23.65804160324708	27.02833949409292	26.623178951946073	22.690439950713923
36	23.565026213428037	30.303689207798794	25.694716242661446	20.436568336111716
37	23.175086371433405	29.964968229808413	26.190717788891305	20.66922760986688
38	22.54354811432437	29.187987726800515	26.28880674542775	21.979657413447367
39	24.333067575076708	27.48713488439516	26.114372689715154	22.065424850812978
40	23.608272329733516	27.354738952912466	27.76376506970114	21.273223647652873
41	20.63781981590201	28.345775651711726	28.106834819163584	22.90956971322268
42	21.64890918315576	28.496533062743108	27.107100577420212	22.74745717668092
43	23.644270493585562	26.745910946824186	27.11289893938296	22.496919620207294
44	23.270276146988476	27.56372158198642	26.388103694039767	22.777898576985333
45	22.507066753642096	27.16194341765118	26.084656084656082	24.246333744050638
46	22.171728153463313	28.8823657316808	25.61740474982484	23.328501365031045
47	22.574472711459013	27.240462902563362	28.116015558937935	22.069048827039694
48	22.35171897272354	26.175980285569327	28.363895532845305	23.10840520886183
49	21.760286052523494	26.813316904641106	28.03024812157232	23.396148921263077
50	20.41168369935493	26.88724601966611	28.4711652291561	24.229905051822858
51	20.907443647169675	26.24870140851876	26.690584909762993	26.153270034548576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	501.0
2	842.0
3	1213.5
4	1585.0
5	1294.5
6	1004.0
7	873.0
8	742.0
9	746.5
10	751.0
11	778.0
12	805.0
13	835.5
14	866.0
15	813.5
16	761.0
17	863.0
18	965.0
19	999.5
20	1034.0
21	1243.0
22	1452.0
23	1753.5
24	2055.0
25	2376.0
26	3745.5
27	4794.0
28	5769.0
29	6744.0
30	7819.0
31	8894.0
32	10155.5
33	11417.0
34	12394.0
35	13371.0
36	14050.5
37	14730.0
38	15754.0
39	16778.0
40	19142.5
41	21507.0
42	23000.0
43	24493.0
44	26790.5
45	29088.0
46	31644.0
47	34200.0
48	34230.5
49	34261.0
50	33206.5
51	32152.0
52	29051.0
53	25950.0
54	23834.5
55	21719.0
56	20678.5
57	19638.0
58	19507.5
59	19377.0
60	18608.5
61	17840.0
62	16103.0
63	14366.0
64	12061.0
65	9756.0
66	7928.0
67	6100.0
68	4976.5
69	3853.0
70	3408.0
71	2963.0
72	2424.0
73	1885.0
74	1474.5
75	817.5
76	571.0
77	425.5
78	280.0
79	228.0
80	176.0
81	140.0
82	104.0
83	82.0
84	60.0
85	41.0
86	22.0
87	17.5
88	13.0
89	11.5
90	10.0
91	8.0
92	6.0
93	5.5
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.61653235899135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39430782629614	26.508285151283534
2	8.80906464150623	6.451148009963188
3	4.168980129209404	4.579607874155643
4	2.6951332105291526	3.947457296605318
5	1.9324541523040588	3.537988500005438
6	1.386196840164719	3.045463287229379
7	1.063150515557011	2.7250219678760135
8	0.816292862993468	2.391185122577121
9	0.6702589891249313	2.208830396777809
>10	5.9865953809511385	41.531843672005905
>50	0.055216084021669234	1.331032505483189
>100	0.021034698674921613	1.489649218161927
>500	0.0013146686671826008	0.25248699787556333
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	528	0.12756396318040153	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.12587277427460075	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	499	0.12055760914208404	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	485	0.11717523133048247	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06813075306225991	0.0
2	0.0	0.0	0.0	0.34137856055664273	0.0
3	0.0	0.0	0.0	0.47860646034162013	0.0
4	0.0	0.0	0.0	0.6714019956029088	0.0
5	0.0	0.0	0.0	1.2932763161073664	0.0
6	0.0	0.0	0.0	1.6795921818752868	0.0
7	0.0	0.0	0.0	2.0758135826628976	0.0
8	0.0	0.0	0.0	2.8201782996303546	0.0
9	0.0	0.0	0.0	3.093426107124737	0.0
10	0.0	0.0	0.0	3.9136527264381145	0.0
11	0.0	0.0	0.0	4.730980164770119	0.0
12	0.0	0.0	0.0	5.4780024643038345	0.0
13	0.0	0.0	0.0	5.785798845159576	0.0
14	0.0	0.0	0.0	5.9090140368679185	0.0
15	0.0	0.0	0.0	6.142398105868425	0.0
16	0.0	0.0	0.0	6.620038172549588	0.0
17	0.0	0.0	0.0	7.16411780338721	0.0
18	0.0	0.0	0.0	7.778019376192892	0.0
19	0.0	0.0	0.0	8.094513299992752	0.0
20	0.0	0.0	0.0	8.461259694136407	0.0
21	0.0	0.0	0.0	8.877050566548283	0.0
22	0.0	0.0	0.0	9.338745137831896	0.0
23	0.0	0.0	0.0	9.80382208692711	0.0
24	0.0	0.0	0.0	10.17032688265565	0.0
25	0.0	0.0	0.0	10.494310357324055	0.0
26	0.0	0.0	0.0	10.788577226933391	0.0
27	0.0	0.0	0.0	11.081152907636925	0.0
28	2.4159841511439684E-4	0.0	0.0	11.410451547437848	0.0
29	2.4159841511439684E-4	0.0	0.0	11.747239738107318	0.0
30	2.4159841511439684E-4	0.0	0.0	12.126790848252035	0.0
31	2.4159841511439684E-4	0.0	0.0	12.50126839167935	0.0
32	2.4159841511439684E-4	0.0	0.0	12.846512526877824	0.0
33	2.4159841511439684E-4	0.0	0.0	13.198279819284386	0.0
34	2.4159841511439684E-4	0.0	0.0	13.55077190693629	0.0
35	2.4159841511439684E-4	0.0	0.0	13.94747650455413	0.0
36	2.4159841511439684E-4	0.0	0.0	14.327510811529077	0.0
37	2.4159841511439684E-4	0.0	0.0	14.678070111860066	0.0
38	2.4159841511439684E-4	0.0	0.0	15.039742939286318	0.0
39	2.4159841511439684E-4	0.0	0.0	15.390785436447537	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.028049E-4	45.000004	25
CTTAACG	20	7.028049E-4	45.000004	12
CTCACGA	20	7.028049E-4	45.000004	24
GATCGCC	20	7.028049E-4	45.000004	20
CGCATCG	20	7.028049E-4	45.000004	21
TAACGTT	20	7.028049E-4	45.000004	14
CGCTGAT	20	7.028049E-4	45.000004	43
GTCCGTT	30	2.1621217E-6	45.000004	9
ACCCGTC	20	7.028049E-4	45.000004	36
CGCCGTA	20	7.028049E-4	45.000004	35
AATGTCG	20	7.028049E-4	45.000004	27
ACAACGG	25	3.886465E-5	45.0	2
CGACATA	25	3.886465E-5	45.0	8
GCCGATG	50	1.0786607E-9	40.5	9
TCGACAC	45	1.9232175E-8	40.0	34
ACGGGAG	245	0.0	39.489796	5
AAGCACG	40	3.4510776E-7	39.375004	1
TACGGGA	195	0.0	39.23077	4
TCACGGG	70	0.0	38.57143	3
ACGTACG	35	6.2386043E-6	38.57143	1
>>END_MODULE