##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936279.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620258
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.025107294061506	33.0	31.0	34.0	30.0	34.0
2	32.14625688020146	33.0	31.0	34.0	30.0	34.0
3	31.97357389989327	33.0	31.0	34.0	30.0	34.0
4	35.75647230668528	37.0	35.0	37.0	35.0	37.0
5	31.40883471071715	37.0	35.0	37.0	0.0	37.0
6	33.53940134589155	37.0	35.0	37.0	17.0	37.0
7	29.294427480177603	35.0	32.0	37.0	0.0	37.0
8	32.33312428054132	35.0	33.0	37.0	17.0	37.0
9	36.36865143214598	39.0	35.0	39.0	32.0	39.0
10	36.940985847824614	38.0	37.0	39.0	34.0	39.0
11	37.093288599260305	39.0	37.0	39.0	34.0	39.0
12	37.02790290492021	39.0	37.0	39.0	33.0	39.0
13	36.776741291527074	39.0	35.0	39.0	33.0	39.0
14	37.97256464245524	40.0	37.0	41.0	33.0	41.0
15	38.04127314762567	40.0	37.0	41.0	33.0	41.0
16	38.1712497057676	40.0	37.0	41.0	33.0	41.0
17	38.100111566477175	40.0	37.0	41.0	33.0	41.0
18	37.94332842139884	40.0	37.0	41.0	33.0	41.0
19	37.8288663749601	40.0	37.0	41.0	33.0	41.0
20	37.77869370487765	40.0	36.0	41.0	33.0	41.0
21	37.75381534780688	40.0	36.0	41.0	33.0	41.0
22	37.794593540107506	40.0	36.0	41.0	33.0	41.0
23	37.78358682999655	39.0	36.0	41.0	33.0	41.0
24	37.690757716950046	39.0	36.0	41.0	33.0	41.0
25	37.56638366615183	39.0	35.0	41.0	33.0	41.0
26	37.35499098762128	39.0	35.0	41.0	33.0	41.0
27	37.36777115329427	39.0	35.0	41.0	33.0	41.0
28	37.27747646946916	39.0	35.0	41.0	32.0	41.0
29	37.12995721135398	39.0	35.0	41.0	32.0	41.0
30	36.98384059536515	39.0	35.0	41.0	31.0	41.0
31	36.859442361082	39.0	35.0	41.0	31.0	41.0
32	36.6336331010644	39.0	35.0	41.0	31.0	41.0
33	36.46688636019205	39.0	35.0	40.0	30.0	41.0
34	36.13715582870354	39.0	35.0	40.0	29.0	41.0
35	36.047796239629314	39.0	35.0	40.0	29.0	41.0
36	35.9294277542571	39.0	35.0	40.0	29.0	41.0
37	35.72842913755244	39.0	35.0	40.0	27.0	41.0
38	35.42479903524018	38.0	34.0	40.0	25.0	41.0
39	34.99369939605777	38.0	34.0	40.0	23.0	41.0
40	34.91495635687085	38.0	34.0	40.0	23.0	41.0
41	34.65491940450587	38.0	34.0	40.0	22.0	41.0
42	34.7435712235876	38.0	34.0	40.0	23.0	41.0
43	34.511878927801014	38.0	33.0	40.0	22.0	41.0
44	34.26091723121669	38.0	33.0	40.0	21.0	41.0
45	34.1197791886602	37.0	33.0	40.0	20.0	41.0
46	33.859419789829396	37.0	33.0	40.0	19.0	41.0
47	33.74159785121675	37.0	33.0	40.0	18.0	41.0
48	33.52901050853032	37.0	33.0	40.0	18.0	41.0
49	33.42735926017883	37.0	32.0	40.0	18.0	41.0
50	33.34874036288125	37.0	32.0	40.0	18.0	41.0
51	31.07162503345382	35.0	28.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	31.0
10	34.0
11	27.0
12	14.0
13	25.0
14	34.0
15	40.0
16	80.0
17	169.0
18	294.0
19	604.0
20	967.0
21	1559.0
22	2425.0
23	3650.0
24	5642.0
25	7810.0
26	9765.0
27	11232.0
28	11898.0
29	13494.0
30	15310.0
31	18601.0
32	22917.0
33	31093.0
34	44124.0
35	51259.0
36	60782.0
37	92218.0
38	111371.0
39	102760.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69036755672639	19.688258756839897	23.8213775557913	12.79999613064241
2	32.06923570514205	27.738618445872525	23.689335728035754	16.50281012094967
3	30.78283552973118	27.924025163722195	25.364445117999285	15.928694188547347
4	27.11532942743181	23.41848069674232	30.9448648788085	18.52132499701737
5	23.75398624443377	36.53382947096208	21.77464861396387	17.93753567064028
6	24.87335915054703	35.33529595748866	25.922599950343244	13.868744941621067
7	65.52273408807304	19.67036297798658	10.78793018389122	4.018972750049174
8	78.56005081756301	7.258431168965172	10.211234679762292	3.9702833337095207
9	72.98672487900197	6.3623524404360765	10.871121372074201	9.779801308487759
10	41.84613499543738	24.739382643996528	18.767996543373886	14.6464858171922
11	31.921232777328147	23.959707089630445	25.69866087982743	18.420399253213983
12	28.775445056734455	21.981981691489672	29.421627774248783	19.820945477527093
13	25.081982013936138	22.277342654185837	33.57925250460293	19.061422827275102
14	21.005936239435847	26.36773729641536	30.875861335121833	21.750465129026953
15	19.832714773529723	23.95890097346588	36.359224709717566	19.849159543286827
16	24.477717336979126	25.759925708334276	28.353846302667602	21.408510652018997
17	25.27528867019853	23.99404763824086	28.736267811136656	21.99439588042395
18	24.85917150605071	25.399591782774266	29.286683928300803	20.454552782874224
19	24.940911685137475	26.151536941079357	27.113233525403945	21.794317848379222
20	25.042643545105424	28.02849781864966	28.96230278368034	17.96655585256458
21	24.959613580155356	27.293158653334583	29.448068384446476	18.299159382063593
22	24.23749471993912	21.98327147735297	29.974784686372445	23.804449116335462
23	22.593178967461927	26.415136926891712	30.388483502026574	20.603200603619783
24	24.58541445656485	24.394364925563234	27.759577466151182	23.260643151720735
25	23.373338191526752	28.513296080018314	27.019885273547462	21.093480454907475
26	22.49273689335728	25.57596999958082	27.775538566209544	24.155754540852357
27	23.36173011875703	25.866977934988345	28.16279677166598	22.60849517458864
28	20.302196827771667	25.091171738212164	30.29158833904601	24.315043094970157
29	23.64354832988853	25.173718033463494	29.234286377604157	21.948447259043817
30	22.860487087631277	24.927046487106978	28.834775206446352	23.377691218815396
31	25.189679133521857	23.164554104904735	27.648010988975557	23.997755772597852
32	27.3110544321879	24.63055696178042	26.737583392717223	21.320805213314458
33	23.43702136852729	23.378336111747046	26.751448590747724	26.433193928977943
34	23.01235937303509	23.26193293758404	32.33477036974936	21.390937319631508
35	22.082262542361406	24.365667190104762	29.7416558915806	23.810414375953233
36	22.50386129642826	24.869006123258387	29.880630318351397	22.746502261961957
37	23.452337575654003	25.239980782190635	28.76045129607357	22.547230346081793
38	22.314101551289948	25.19258115171429	26.541535941495315	25.951781355500454
39	24.66731585888453	23.721741597851217	28.721112827242855	22.8898297160214
40	23.73705780497793	23.342544554040416	30.473770592237425	22.446627048744233
41	21.188924608791826	24.69794827313795	30.17454027195135	23.938586846118874
42	21.478965204801874	23.51956766377862	32.387490366911834	22.613976764507672
43	23.205827252530398	23.277087921477836	29.341338604258226	24.175746221733537
44	23.192123277732815	23.735929242347538	28.921029636054673	24.15091784386497
45	22.068236121097996	23.38317280873443	28.546830512464165	26.001760557703406
46	24.03435344646905	24.76276001276888	27.47759803178677	23.725288508975297
47	20.94983055438221	23.57051420537905	32.51002002392553	22.96963521631321
48	22.295238433039156	25.028455900609103	28.893138016760766	23.78316764959098
49	22.4967674741801	23.10796475015236	30.596461472484034	23.798806303183515
50	21.51798122716676	23.69320508562566	30.129881436434513	24.658932250773066
51	22.048405663449724	23.60598331661986	28.031561060075	26.314049959855414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	609.5
2	1012.0
3	3466.0
4	5920.0
5	4495.0
6	3070.0
7	2464.0
8	1858.0
9	1815.0
10	1772.0
11	1704.5
12	1637.0
13	1634.0
14	1631.0
15	1597.5
16	1564.0
17	1520.5
18	1477.0
19	1596.0
20	1715.0
21	1870.0
22	2025.0
23	2354.0
24	2683.0
25	3305.5
26	4603.0
27	5278.0
28	6631.0
29	7984.0
30	8583.5
31	9183.0
32	10943.5
33	12704.0
34	14381.0
35	16058.0
36	17504.0
37	18950.0
38	20462.0
39	21974.0
40	24539.0
41	27104.0
42	30351.0
43	33598.0
44	39018.0
45	44438.0
46	52631.5
47	60825.0
48	54195.0
49	47565.0
50	46130.5
51	44696.0
52	42426.5
53	40157.0
54	38052.0
55	35947.0
56	34334.5
57	32722.0
58	31783.5
59	30845.0
60	29098.5
61	27352.0
62	24456.0
63	21560.0
64	19354.5
65	17149.0
66	14519.0
67	11889.0
68	9965.0
69	8041.0
70	6767.5
71	5494.0
72	4457.0
73	3420.0
74	2810.0
75	1756.5
76	1313.0
77	973.0
78	633.0
79	463.5
80	294.0
81	237.0
82	180.0
83	130.0
84	80.0
85	62.5
86	45.0
87	48.0
88	51.0
89	33.5
90	16.0
91	11.0
92	6.0
93	4.0
94	2.0
95	2.5
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.49932802591223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41505772796249	27.905146598080606
2	7.974960742446238	5.981113377495281
3	3.828420272792204	4.306895628914617
4	2.4661115036501564	3.6991009689541143
5	1.7430350883252261	3.268132226889127
6	1.2630693272391413	2.8418550612965303
7	0.9601858088494756	2.520442582831064
8	0.8350788802174596	2.5051917485428783
9	0.6621905578371867	2.2348530849598625
>10	5.793000376117772	38.93236340933368
>50	0.038079063554375334	0.9992254276483673
>100	0.01461173368666925	1.0862301008196267
>500	0.0035422384694955757	0.9799648509687305
>1k	0.002656678852121682	2.7394849332655182
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	4725	0.7617797755127705	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	3635	0.5860464516378668	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	3320	0.5352611332703489	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2474	0.39886627822615756	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTT	1273	0.20523717549793796	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	1072	0.17283130568247407	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT	948	0.15283962480129235	No Hit
GAATATGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	876	0.14123155203157398	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.1291398095631173	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	797	0.12849491663146625	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC	651	0.10495632462620393	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	632	0.10189308320086157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6122323291275565E-4	0.0	0.0	0.041918040557316474	0.0
2	1.6122323291275565E-4	0.0	0.0	0.3653318457803043	0.0
3	1.6122323291275565E-4	0.0	0.0	0.48125135024457566	0.0
4	1.6122323291275565E-4	0.0	0.0	0.804987601933389	0.0
5	1.6122323291275565E-4	0.0	0.0	2.4673603564968127	0.0
6	1.6122323291275565E-4	0.0	0.0	2.94635458148061	0.0
7	1.6122323291275565E-4	0.0	0.0	3.547717240245188	0.0
8	1.6122323291275565E-4	0.0	0.0	4.222275246752158	0.0
9	1.6122323291275565E-4	0.0	0.0	4.465238658751681	0.0
10	1.6122323291275565E-4	0.0	0.0	6.483431088353556	0.0
11	1.6122323291275565E-4	0.0	0.0	7.115748607837384	0.0
12	1.6122323291275565E-4	0.0	0.0	8.647852990207301	0.0
13	1.6122323291275565E-4	0.0	0.0	8.897426554756247	0.0
14	1.6122323291275565E-4	0.0	0.0	9.036723427992868	0.0
15	1.6122323291275565E-4	0.0	0.0	9.285813322843074	0.0
16	1.6122323291275565E-4	0.0	0.0	9.578917160278465	0.0
17	1.6122323291275565E-4	0.0	0.0	9.894914696787465	0.0
18	1.6122323291275565E-4	0.0	0.0	10.247509907167663	0.0
19	1.6122323291275565E-4	0.0	0.0	10.471932647382218	0.0
20	1.6122323291275565E-4	0.0	0.0	10.692324806773955	0.0
21	1.6122323291275565E-4	0.0	0.0	10.965114516862338	0.0
22	1.6122323291275565E-4	0.0	0.0	11.252736764378694	0.0
23	1.6122323291275565E-4	0.0	0.0	11.532459073482325	0.0
24	1.6122323291275565E-4	0.0	0.0	11.771069458193203	0.0
25	1.6122323291275565E-4	0.0	0.0	11.983722902405129	0.0
26	1.6122323291275565E-4	0.0	0.0	12.212337446675416	0.0
27	1.6122323291275565E-4	0.0	0.0	12.465457922348442	0.0
28	1.6122323291275565E-4	0.0	0.0	12.691976564590863	0.0
29	1.6122323291275565E-4	0.0	0.0	12.93574609275495	0.0
30	1.6122323291275565E-4	0.0	0.0	13.257547665648811	0.0
31	1.6122323291275565E-4	0.0	0.0	13.51792318680291	0.0
32	1.6122323291275565E-4	0.0	0.0	13.752986660389709	0.0
33	1.6122323291275565E-4	0.0	0.0	14.004333680500695	0.0
34	1.6122323291275565E-4	0.0	0.0	14.27260914006752	0.0
35	1.6122323291275565E-4	0.0	0.0	14.555717137062318	0.0
36	1.6122323291275565E-4	0.0	0.0	14.805451924844178	0.0
37	1.6122323291275565E-4	0.0	0.0	15.087270135975675	0.0
38	1.6122323291275565E-4	0.0	0.0	15.39762485933273	0.0
39	1.6122323291275565E-4	0.0	0.0	15.895965872266057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	20	7.0307724E-4	45.0	12
CGTTAGC	20	7.0307724E-4	45.0	37
TATTACG	20	7.0307724E-4	45.0	1
ACTCGCA	20	7.0307724E-4	45.0	11
ATGCGAA	25	3.8887225E-5	45.0	14
CGTTATT	215	0.0	41.860466	1
CGTTTTT	1005	0.0	41.19403	1
CGTTTTA	115	0.0	41.08696	1
CAAACGG	55	6.002665E-11	40.909092	2
TACGGCT	550	0.0	40.909092	7
GCGATAC	50	1.0804797E-9	40.5	9
AACGGCT	130	0.0	39.80769	7
TAATACG	85	0.0	39.705883	4
TGATACC	890	0.0	39.69101	4
ATAGCGG	40	3.4547884E-7	39.375	2
TCGACGG	40	3.4547884E-7	39.375	2
AAACGGC	155	0.0	39.193546	6
ACGGCTG	720	0.0	38.125004	8
GATACCT	935	0.0	38.02139	5
GACACGA	95	0.0	37.894737	25
>>END_MODULE