##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485336
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00726713040038	33.0	31.0	34.0	30.0	34.0
2	32.15888374239702	33.0	31.0	34.0	30.0	34.0
3	32.075961807902154	33.0	31.0	34.0	30.0	34.0
4	35.789576705622494	37.0	35.0	37.0	35.0	37.0
5	31.247175152883777	37.0	35.0	37.0	0.0	37.0
6	33.46295556068373	37.0	35.0	37.0	17.0	37.0
7	29.51669977088038	35.0	32.0	37.0	0.0	37.0
8	32.558260668897425	35.0	33.0	37.0	17.0	37.0
9	36.46231682792952	39.0	35.0	39.0	32.0	39.0
10	36.88045601397795	38.0	37.0	39.0	33.0	39.0
11	37.039797171444114	39.0	37.0	39.0	34.0	39.0
12	37.081994741787135	39.0	37.0	39.0	34.0	39.0
13	36.942347157433204	39.0	37.0	39.0	33.0	39.0
14	38.254155883758884	40.0	38.0	41.0	33.0	41.0
15	38.33837382761633	40.0	38.0	41.0	34.0	41.0
16	38.37982758336493	40.0	38.0	41.0	34.0	41.0
17	38.357383338553085	40.0	38.0	41.0	34.0	41.0
18	38.261874247943695	40.0	37.0	41.0	34.0	41.0
19	38.18372014439481	40.0	37.0	41.0	34.0	41.0
20	38.111561887022596	40.0	37.0	41.0	34.0	41.0
21	38.03487686880841	40.0	37.0	41.0	33.0	41.0
22	38.03328209735111	40.0	37.0	41.0	33.0	41.0
23	37.974522804819756	40.0	37.0	41.0	33.0	41.0
24	37.924979807803254	40.0	37.0	41.0	33.0	41.0
25	37.80458692534656	40.0	36.0	41.0	33.0	41.0
26	37.61488947862924	40.0	36.0	41.0	33.0	41.0
27	37.591649908516985	40.0	36.0	41.0	33.0	41.0
28	37.48682974269372	40.0	36.0	41.0	33.0	41.0
29	37.47100359338685	40.0	36.0	41.0	33.0	41.0
30	37.37360715051016	40.0	36.0	41.0	32.0	41.0
31	37.278438030560274	40.0	36.0	41.0	32.0	41.0
32	37.17380948456327	40.0	36.0	41.0	32.0	41.0
33	37.01320940544283	39.0	35.0	41.0	31.0	41.0
34	36.889453904099426	39.0	35.0	41.0	31.0	41.0
35	36.80969884780853	39.0	35.0	41.0	31.0	41.0
36	36.70056620568019	39.0	35.0	41.0	30.0	41.0
37	36.59801251092027	39.0	35.0	41.0	30.0	41.0
38	36.52027049301927	39.0	35.0	41.0	30.0	41.0
39	36.520548650831586	39.0	35.0	41.0	30.0	41.0
40	36.33833673990802	39.0	35.0	41.0	30.0	41.0
41	36.23122125702606	39.0	35.0	40.0	30.0	41.0
42	36.19229564672722	39.0	35.0	40.0	29.0	41.0
43	36.03151837077818	39.0	35.0	40.0	29.0	41.0
44	35.839929450937085	39.0	35.0	40.0	28.0	41.0
45	35.77351154663985	38.0	35.0	40.0	28.0	41.0
46	35.67365907330179	38.0	35.0	40.0	27.0	41.0
47	35.55609515881781	38.0	34.0	40.0	27.0	41.0
48	35.4709644452503	38.0	34.0	40.0	27.0	41.0
49	35.31457794187944	38.0	34.0	40.0	26.0	41.0
50	35.17885753374981	38.0	34.0	40.0	26.0	41.0
51	33.25976436942654	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	25.0
10	35.0
11	33.0
12	20.0
13	14.0
14	26.0
15	32.0
16	54.0
17	127.0
18	218.0
19	340.0
20	539.0
21	889.0
22	1286.0
23	1939.0
24	2576.0
25	3717.0
26	4662.0
27	5569.0
28	6355.0
29	7560.0
30	9236.0
31	12023.0
32	15784.0
33	21836.0
34	31620.0
35	38817.0
36	47600.0
37	75603.0
38	97906.0
39	98869.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41069279839122	21.23209487859957	25.635848154680467	11.721364168328744
2	31.59769726539964	25.901025269091924	25.24292448942588	17.25835297608255
3	32.36767929846539	25.012774655084314	25.883305586233043	16.736240460217253
4	29.50965928758633	26.62650205218653	25.62163119982857	18.242207460398568
5	22.83634430580052	39.855481563288116	20.591301696144527	16.71687243476684
6	26.557889791814333	35.92665699639013	23.489912143339872	14.025541068455668
7	69.8804539535497	19.666169416651556	6.552985972604546	3.9003906571941913
8	83.57282377569354	6.420088351162906	6.835058598579129	3.172029274564425
9	76.94463217235071	9.284289646760183	8.089859396376943	5.681218784512173
10	39.87340728897094	31.173661133730036	15.733017950450822	13.219913626848204
11	31.044678325943266	26.786597326388318	24.670125438871217	17.498598908797206
12	30.578609458189792	23.65660078790776	27.451909604892283	18.31288014901017
13	25.716410898841215	24.751924439975603	27.785492936851995	21.746171724331184
14	20.13141411310927	28.734526183922064	27.740369558409018	23.393690144559645
15	19.977500123625695	28.46007714243328	32.03244762391416	19.529975110026868
16	22.796990126427875	26.957200784611075	30.318789457200783	19.927019631760263
17	22.81079499563189	26.969975439695386	27.610562579326487	22.608666985346236
18	22.222542733281685	29.178754512337846	27.30417690012692	21.29452585425355
19	25.66654853544761	28.63789209949396	25.254462887566554	20.441096477491882
20	27.85802001087906	27.19827088862149	26.67512815863649	18.268580941862957
21	26.043606903258777	27.6223070202911	26.34360525491618	19.99048082153395
22	24.53207674683106	26.69490826973478	26.035571233125093	22.737443750309065
23	25.23303443387674	27.619010335108047	27.05733759704617	20.090617633969043
24	23.2902566469415	26.16393591244004	28.291328069625994	22.254479370992467
25	23.940527799297804	28.651284883050092	25.71455651342575	21.69363080422635
26	22.956055186509964	30.10553513442234	25.492236306393924	21.446173372673776
27	23.16436448151384	29.131364662831523	27.176224306459858	20.528046549194784
28	21.49438739347586	30.003749979395717	27.628282262185373	20.873580364943052
29	22.9857253531574	28.313168608963686	27.41708836764633	21.28401767023258
30	23.110381261641418	28.070862248009625	26.875607826330622	21.94314866401833
31	23.967931494881896	28.95540409118631	25.51469497420344	21.561969439728355
32	25.210575766067215	28.68239734946511	25.83179488024791	20.275232004219756
33	23.48125834473437	29.582392404437343	25.774514975192442	21.161834275635847
34	23.081947351937625	28.698674732556412	26.84119867473256	21.378179240773402
35	23.897670891918178	27.404931841033843	26.21853726078428	22.4788600062637
36	23.640323404816456	30.185891835759143	24.99958791435212	21.17419684507228
37	23.797946165130963	30.131908615886726	25.950063461189778	20.12008175779254
38	22.749600276921555	28.210765325465243	26.37698419239455	22.662650205218654
39	24.101447244795356	26.999027477871003	26.290858291987405	22.608666985346236
40	23.50042032736084	27.60520546590403	29.022574051790922	19.871800154944204
41	20.676397382431965	29.119208136219033	28.332124548766213	21.87226993258279
42	23.146644798654954	28.46007714243328	26.436530568513362	21.956747490398403
43	23.639705276344635	28.618730116867493	26.43240971203455	21.309154894753327
44	22.789160499118136	28.754924423492177	26.05040631644881	22.405508760940872
45	22.36162163944154	28.0529365223268	25.93275586397877	23.652685974252886
46	22.086348426656997	29.95800847248092	25.711053785418763	22.24458931544332
47	22.954200801094498	27.851426640512965	27.663721627903143	21.530650930489394
48	22.3298910445547	26.783506684029206	28.104241185487993	22.7823610859281
49	20.868017208696653	28.46749468409514	27.723268004021957	22.941220103186247
50	20.21012247185455	27.508159295828044	28.06777160565052	24.213946626666885
51	21.098579128686108	27.753762341965153	26.724578436382217	24.423080092966522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	243.0
1	600.5
2	958.0
3	1618.0
4	2278.0
5	1850.0
6	1422.0
7	1213.5
8	1005.0
9	993.0
10	981.0
11	978.0
12	975.0
13	1055.0
14	1135.0
15	1024.0
16	913.0
17	1138.0
18	1363.0
19	1368.5
20	1374.0
21	1532.0
22	1690.0
23	1972.5
24	2255.0
25	3098.0
26	4736.5
27	5532.0
28	7045.0
29	8558.0
30	9736.0
31	10914.0
32	12801.0
33	14688.0
34	16337.5
35	17987.0
36	19008.0
37	20029.0
38	21540.5
39	23052.0
40	24375.0
41	25698.0
42	27372.5
43	29047.0
44	30711.0
45	32375.0
46	34497.0
47	36619.0
48	35054.0
49	33489.0
50	32550.0
51	31611.0
52	29613.0
53	27615.0
54	26101.0
55	24587.0
56	23908.0
57	23229.0
58	23773.5
59	24318.0
60	23687.0
61	23056.0
62	21001.0
63	18946.0
64	15514.0
65	12082.0
66	9632.5
67	7183.0
68	6151.0
69	5119.0
70	4233.0
71	3347.0
72	2889.5
73	2432.0
74	1918.5
75	1090.0
76	775.0
77	652.0
78	529.0
79	398.0
80	267.0
81	193.5
82	120.0
83	106.5
84	93.0
85	73.5
86	54.0
87	36.5
88	19.0
89	14.5
90	10.0
91	8.0
92	6.0
93	7.5
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485336.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.73071382753942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.52578228829354	23.45154266002238
2	8.952391197056405	6.039410910801853
3	4.652131207275851	4.707591193223616
4	3.129919181854372	4.222976329058245
5	2.207297817513682	3.7226865507353164
6	1.6227017894801097	3.284093381303381
7	1.2207168814123306	2.882295625395598
8	0.9660328265336844	2.6067981455853384
9	0.7537687255858748	2.288264145739761
>10	6.887070317042376	43.06679144535038
>50	0.05235665217667392	1.216299931496268
>100	0.024960729526088726	1.4027595529378702
>500	0.004870386248992922	1.1084901283499669
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG	843	0.17369410058186494	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	771	0.15885901725814694	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.15824088878632533	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC	691	0.1423755913429047	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	667	0.13743056356833203	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	583	0.1201229663573277	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	566	0.11662023835033873	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	509	0.10487579738572865	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.060428239405278E-4	0.0	0.0	0.03749979395717606	0.0
2	2.060428239405278E-4	0.0	0.0	0.17390014340580545	0.0
3	2.060428239405278E-4	0.0	0.0	0.24416074636952545	0.0
4	2.060428239405278E-4	0.0	0.0	0.36572601249443687	0.0
5	2.060428239405278E-4	0.0	0.0	0.8130449832693227	0.0
6	2.060428239405278E-4	0.0	0.0	1.0495821451530487	0.0
7	2.060428239405278E-4	0.0	0.0	1.2927126774028714	0.0
8	2.060428239405278E-4	0.0	0.0	1.687696770896863	0.0
9	2.060428239405278E-4	0.0	0.0	1.838314075197389	0.0
10	2.060428239405278E-4	0.0	0.0	2.4327476222658118	0.0
11	2.060428239405278E-4	0.0	0.0	2.9319893846737104	0.0
12	2.060428239405278E-4	0.0	0.0	3.49386816555953	0.0
13	2.060428239405278E-4	0.0	0.0	3.6681803946132163	0.0
14	2.060428239405278E-4	0.0	0.0	3.7431799825275687	0.0
15	2.060428239405278E-4	0.0	0.0	3.899360443074489	0.0
16	2.060428239405278E-4	0.0	0.0	4.191323124598217	0.0
17	2.060428239405278E-4	0.0	0.0	4.507186773699045	0.0
18	2.060428239405278E-4	0.0	0.0	4.839327805891177	0.0
19	2.060428239405278E-4	0.0	0.0	5.043104158768358	0.0
20	2.060428239405278E-4	0.0	0.0	5.262127680617139	0.0
21	2.060428239405278E-4	0.0	0.0	5.5384311075213875	0.0
22	2.060428239405278E-4	0.0	0.0	5.837193202235152	0.0
23	2.060428239405278E-4	0.0	0.0	6.1182356140900325	0.0
24	2.060428239405278E-4	0.0	0.0	6.349827748199186	0.0
25	2.060428239405278E-4	0.0	0.0	6.557724957555179	0.0
26	2.060428239405278E-4	0.0	0.0	6.74563601298894	0.0
27	2.060428239405278E-4	0.0	0.0	6.943231081147906	0.0
28	2.060428239405278E-4	0.0	0.0	7.152982675919363	0.0
29	2.060428239405278E-4	0.0	0.0	7.3765391398948355	0.0
30	2.060428239405278E-4	0.0	0.0	7.6171971582573725	0.0
31	2.060428239405278E-4	0.0	0.0	7.85043763495805	0.0
32	2.060428239405278E-4	0.0	0.0	8.074612227405344	0.0
33	2.060428239405278E-4	0.0	0.0	8.294253877725946	0.0
34	2.060428239405278E-4	0.0	0.0	8.530791039609673	0.0
35	2.060428239405278E-4	0.0	0.0	8.78793248388745	0.0
36	2.060428239405278E-4	0.0	0.0	9.058672754545304	0.0
37	2.060428239405278E-4	0.0	0.0	9.323025697661002	0.0
38	2.060428239405278E-4	0.0	0.0	9.571307300509337	0.0
39	2.060428239405278E-4	0.0	0.0	9.824327888308305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	30	2.1629003E-6	45.000004	2
CGACGGA	20	7.0292526E-4	45.000004	28
CCAATCC	20	7.0292526E-4	45.000004	16
CGTTCGC	20	7.0292526E-4	45.000004	45
TAGTTCG	20	7.0292526E-4	45.000004	15
GTAGACG	30	2.1629003E-6	45.000004	1
ACCCGCC	20	7.0292526E-4	45.000004	37
CGGTAGT	20	7.0292526E-4	45.000004	12
TCGACGG	35	1.2099918E-7	45.000004	2
TAGCACG	25	3.8874627E-5	45.0	1
GCGATAT	25	3.8874627E-5	45.0	9
ACGGGTA	60	3.6379788E-12	41.250004	5
TACGGGC	55	6.002665E-11	40.909092	4
AGGCGAT	140	0.0	40.178574	7
CGGGCTT	40	3.4527147E-7	39.375004	6
TAACGGG	155	0.0	39.193546	3
AACGGGT	75	0.0	39.0	4
CTTAACG	35	6.240829E-6	38.57143	1
TATGCGG	35	6.240829E-6	38.57143	2
ACGGGCT	70	0.0	38.57143	5
>>END_MODULE