SRR2936271.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368574
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	1014	0.2751143596672581	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	850	0.2306185460721592	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	807	0.2189519608002735	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	696	0.18883589184261507	Illumina PCR Primer Index 11 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	502	0.13620060015085167	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	419	0.1136813774167467	Illumina PCR Primer Index 11 (95% over 23bp)
TAGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTTTAA	400	0.10852637462219256	No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	395	0.10716979493941516	Illumina PCR Primer Index 11 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGCA	35	1.2089185E-7	45.000004	10
GGTACCC	20	7.027043E-4	45.0	37
CGAACTA	20	7.027043E-4	45.0	22
GTCGATG	20	7.027043E-4	45.0	1
CTCCGAT	20	7.027043E-4	45.0	42
TTTCGTC	25	3.885631E-5	45.0	16
TGGCGCG	20	7.027043E-4	45.0	1
TAAACGG	25	3.885631E-5	45.0	2
ACGGGCA	45	3.8380676E-10	45.0	5
CGTTAAC	20	7.027043E-4	45.0	25
CCACTAG	20	7.027043E-4	45.0	42
CACTAGA	20	7.027043E-4	45.0	43
CTTTGCG	20	7.027043E-4	45.0	1
TCCGTTA	20	7.027043E-4	45.0	23
TTACGCG	20	7.027043E-4	45.0	1
GCTACGA	90	0.0	45.0	10
TCGATGG	30	2.1614742E-6	44.999996	2
CGTCATA	30	2.1614742E-6	44.999996	38
CGGGAGT	100	0.0	42.75	6
AATATAC	95	0.0	42.63158	16