##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368574
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71140937776403	31.0	31.0	34.0	30.0	34.0
2	31.859743226597644	31.0	31.0	34.0	30.0	34.0
3	31.823370612143016	31.0	31.0	34.0	30.0	34.0
4	35.553169784086776	37.0	35.0	37.0	33.0	37.0
5	30.98834155420621	37.0	35.0	37.0	0.0	37.0
6	33.236967338987554	37.0	35.0	37.0	17.0	37.0
7	29.604836477885037	35.0	32.0	37.0	0.0	37.0
8	32.57899906124686	35.0	33.0	37.0	17.0	37.0
9	36.328848480902074	39.0	35.0	39.0	32.0	39.0
10	36.63305062212744	38.0	35.0	39.0	32.0	39.0
11	36.76816595853207	39.0	35.0	39.0	33.0	39.0
12	36.787947603466336	39.0	35.0	39.0	33.0	39.0
13	36.54207838860039	39.0	35.0	39.0	32.0	39.0
14	37.72688252562579	39.0	37.0	41.0	33.0	41.0
15	37.843309620320476	40.0	36.0	41.0	33.0	41.0
16	37.90703630749863	40.0	36.0	41.0	33.0	41.0
17	37.8776663573665	40.0	36.0	41.0	33.0	41.0
18	37.78697900557283	39.0	36.0	41.0	33.0	41.0
19	37.72974490875645	39.0	36.0	41.0	33.0	41.0
20	37.68540917156392	39.0	36.0	41.0	33.0	41.0
21	37.60890621693337	39.0	36.0	41.0	33.0	41.0
22	37.59277377134578	39.0	36.0	41.0	33.0	41.0
23	37.56998051951575	39.0	36.0	41.0	33.0	41.0
24	37.50030387384894	39.0	36.0	41.0	32.0	41.0
25	37.39406197941255	39.0	36.0	41.0	32.0	41.0
26	37.21427176089469	39.0	35.0	41.0	32.0	41.0
27	37.152213666726354	39.0	35.0	41.0	32.0	41.0
28	37.05682983607091	39.0	35.0	41.0	31.0	41.0
29	37.0645026507567	39.0	35.0	41.0	31.0	41.0
30	36.94644494728331	39.0	35.0	40.0	31.0	41.0
31	36.80400408059169	39.0	35.0	40.0	31.0	41.0
32	36.746392854623494	39.0	35.0	40.0	31.0	41.0
33	36.617550342672025	39.0	35.0	40.0	30.0	41.0
34	36.502037582683535	39.0	35.0	40.0	30.0	41.0
35	36.441428315616406	39.0	35.0	40.0	30.0	41.0
36	36.31016566551086	39.0	35.0	40.0	30.0	41.0
37	36.20686483582673	39.0	35.0	40.0	30.0	41.0
38	36.14456527047486	38.0	35.0	40.0	30.0	41.0
39	36.130166533721855	38.0	35.0	40.0	30.0	41.0
40	35.994912826189584	38.0	35.0	40.0	29.0	41.0
41	35.93430627228182	38.0	35.0	40.0	29.0	41.0
42	35.867779604638415	38.0	35.0	40.0	29.0	41.0
43	35.69801722313565	38.0	35.0	40.0	28.0	41.0
44	35.505282521284734	38.0	34.0	40.0	27.0	41.0
45	35.34244412248287	38.0	34.0	40.0	26.0	41.0
46	35.18724869361377	38.0	34.0	40.0	26.0	41.0
47	35.01319952031342	38.0	34.0	40.0	25.0	41.0
48	34.95236234785959	38.0	34.0	40.0	25.0	41.0
49	34.74572541741957	38.0	34.0	40.0	24.0	41.0
50	34.550391508896446	37.0	33.0	40.0	23.0	41.0
51	32.338458491374865	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	16.0
10	15.0
11	13.0
12	10.0
13	7.0
14	9.0
15	20.0
16	28.0
17	58.0
18	165.0
19	213.0
20	439.0
21	720.0
22	1111.0
23	1689.0
24	2235.0
25	3062.0
26	3821.0
27	4684.0
28	5701.0
29	6832.0
30	8531.0
31	11133.0
32	14587.0
33	20121.0
34	28021.0
35	33188.0
36	38158.0
37	56684.0
38	66881.0
39	60406.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.379136889742625	22.22837205011748	26.157298127377405	11.235192932762486
2	29.84719486453195	26.152414440519404	26.435125646410217	17.56526504853842
3	31.161449261206702	25.843928220655826	26.303266101244255	16.691356416893218
4	27.58306337397646	25.982299348299122	26.917796697542418	19.516840580182
5	22.384107397700326	38.74147389669375	20.787955742944426	18.0864629626615
6	25.301838979417973	35.21246750991659	25.1176154584968	14.368078052168629
7	69.2596330723274	18.811961777010858	7.438669032541634	4.489736118120106
8	80.74904903764238	5.877245817664838	8.10230781335634	5.2713973313364475
9	74.19161416703294	8.019556452706919	10.260083456782084	7.528745923478053
10	39.99576747138974	26.61690732390239	18.749287795666543	14.638037409041333
11	28.791775871331133	24.80234634021933	26.52465990547353	19.88121788297601
12	27.825619821257057	22.132597524513393	30.534438131826985	19.507344522402555
13	22.5140677313104	25.753308697846293	30.797614590285804	20.9350089805575
14	18.104912446347274	29.491770987644273	30.355640929636923	22.04767563637153
15	17.44344419302501	26.96636225018585	35.80881993846555	19.781373618323595
16	19.80199362950181	26.189584723827508	32.395122824724474	21.613298821946202
17	19.93466712247744	26.00102014792145	29.32029931574121	24.744013413859903
18	21.07012431696213	26.92566485970253	30.720560864304048	21.283649959031294
19	22.39387477141632	28.009029394368568	27.422444339535616	22.174651494679495
20	23.887197686217693	27.14244629301036	28.67972238953372	20.29063363123823
21	22.7218957387119	27.848139043991164	29.32436905478954	20.105596162507393
22	20.603189590150144	25.700402090217978	27.82887561249573	25.867532707136153
23	20.268928356313793	26.991323316348954	29.37591908273508	23.36382924460217
24	21.328417088562947	24.880485329947312	30.54610471709887	23.244992864390866
25	20.127301437431832	28.067362320727995	28.248058734473947	23.55727750736623
26	19.39501972466859	28.259725319745833	27.74178319686142	24.60347175872416
27	20.44338450351897	29.089409453732497	28.727202678430924	21.740003364317616
28	17.59185401032086	28.312089295501043	31.158193469968037	22.937863224210066
29	19.589010619305757	26.034934639990887	29.510763103203157	24.865291637500203
30	19.852187077764576	27.959921209852023	28.778752706376466	23.409139006006935
31	20.484353209938845	30.45874098552801	25.873230341803815	23.18367546272933
32	20.84493208962108	30.470678886736447	25.035135413783934	23.649253609858537
33	19.99788373569487	27.85627852208783	26.466055663177546	25.67978207903976
34	18.344213102389208	28.975185444442637	27.24310450547244	25.437496947695713
35	19.23629990178363	26.94926934618286	26.598457840216618	27.215972911816895
36	20.120518539017944	27.51713360139348	26.907215376016758	25.45513248357182
37	18.869752071497174	30.21130085138941	28.53456836347653	22.38437871363688
38	18.003982917948637	29.242160326013227	26.889851156077206	25.864005599960933
39	19.619940636073082	27.6023268054719	27.45174646068361	25.325986097771413
40	19.828853907220804	25.751138170353848	29.13146342389859	25.288544498526754
41	18.122819298159936	26.22594105932594	27.560001519369244	28.09123812314488
42	19.368973394759262	25.423388518994827	28.44720463190567	26.760433454340244
43	21.036752456765807	25.769587654039622	28.07061811196666	25.12304177722791
44	19.89993868259834	27.003261217557395	27.258840829792657	25.837959270051602
45	19.77838914302148	26.849425081530438	26.219158160912055	27.153027614536025
46	20.692723849213454	27.747209515592523	26.88062641423432	24.679440220959698
47	18.493436867494722	25.812184256078837	30.412074644440466	25.28230423198598
48	18.975022654880703	24.95970958342151	29.00964256838518	27.055625193312604
49	19.191532772251975	24.65013809981171	31.24338667404646	24.914942453889857
50	18.315724929050884	24.770331059705786	30.244401395649177	26.669542615594153
51	17.86316994687634	24.682967328134918	27.775155057057745	29.678707667930997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	339.5
2	561.0
3	794.0
4	1027.0
5	878.5
6	730.0
7	683.0
8	636.0
9	576.5
10	517.0
11	543.5
12	570.0
13	594.0
14	618.0
15	673.5
16	729.0
17	760.5
18	792.0
19	899.0
20	1006.0
21	1052.0
22	1098.0
23	1464.0
24	1830.0
25	2391.0
26	3471.5
27	3991.0
28	4620.0
29	5249.0
30	6152.0
31	7055.0
32	8376.0
33	9697.0
34	10833.5
35	11970.0
36	12516.0
37	13062.0
38	13961.0
39	14860.0
40	17233.5
41	19607.0
42	21760.5
43	23914.0
44	27527.5
45	31141.0
46	35637.0
47	40133.0
48	38984.5
49	37836.0
50	35763.5
51	33691.0
52	28942.0
53	24193.0
54	20849.5
55	17506.0
56	16035.0
57	14564.0
58	13619.0
59	12674.0
60	11568.0
61	10462.0
62	8992.5
63	7523.0
64	6607.0
65	5691.0
66	4730.5
67	3770.0
68	3149.5
69	2529.0
70	2131.5
71	1734.0
72	1404.0
73	1074.0
74	856.5
75	519.5
76	400.0
77	302.0
78	204.0
79	155.0
80	106.0
81	77.0
82	48.0
83	41.0
84	34.0
85	25.5
86	17.0
87	12.5
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.47389130008368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7948244541772	29.86763703467125
2	10.118830123407244	8.190968609975943
3	4.100506757883666	4.9789039438152605
4	2.4641292789443567	3.989316023413899
5	1.6576413052960421	3.3545597002540424
6	1.137979947053746	2.7635086007236978
7	0.8409372072823048	2.3825200782417957
8	0.6480010246898313	2.098169842851126
9	0.4914379004782262	1.7901363748227361
>10	4.669310493056458	36.523917113154354
>50	0.051604527151586664	1.3763297634148788
>100	0.02144603725780225	1.634561126199854
>500	0.002680754657225281	0.7744228369234291
>1k	6.701886643063203E-4	0.2750489515377783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	1014	0.2751143596672581	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	850	0.2306185460721592	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	807	0.2189519608002735	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	696	0.18883589184261507	Illumina PCR Primer Index 11 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	502	0.13620060015085167	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	419	0.1136813774167467	Illumina PCR Primer Index 11 (95% over 23bp)
TAGAGGGGGTTTATTAAGTCCTGTGCCAGCACTTCGCTCATAAAGTTTTAA	400	0.10852637462219256	No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	395	0.10716979493941516	Illumina PCR Primer Index 11 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18910720777917053	0.0
2	0.0	0.0	0.0	0.9097223352705291	0.0
3	0.0	0.0	0.0	1.2220069782458882	0.0
4	0.0	0.0	0.0	1.6349498336833308	0.0
5	0.0	0.0	0.0	3.0476919153277224	0.0
6	0.0	0.0	0.0	3.8304383922902865	0.0
7	0.0	0.0	0.0	4.687254119932496	0.0
8	0.0	0.0	0.0	6.119802264945438	0.0
9	0.0	0.0	0.0	6.6258064866214115	0.0
10	0.0	0.0	0.0	8.090098596211345	0.0
11	0.0	0.0	0.0	9.568499134502163	0.0
12	0.0	0.0	0.0	11.014070444469768	0.0
13	0.0	0.0	0.0	11.542051257006735	0.0
14	0.0	0.0	0.0	11.741739786311568	0.0
15	0.0	0.0	0.0	12.134605262443905	0.0
16	0.0	0.0	0.0	12.935529907155686	0.0
17	0.0	0.0	0.0	13.93804229272819	0.0
18	0.0	0.0	0.0	15.058848426638884	0.0
19	0.0	0.0	0.0	15.701324564402263	0.0
20	0.0	0.0	0.0	16.338917015307644	0.0
21	0.0	0.0	0.0	17.10592716794999	0.0
22	0.0	0.0	0.0	17.995572123915416	0.0
23	0.0	0.0	0.0	18.784016235545643	0.0
24	0.0	0.0	0.0	19.42432184581658	0.0
25	0.0	0.0	0.0	19.983775306993984	0.0
26	0.0	0.0	0.0	20.47919820714429	0.0
27	0.0	0.0	0.0	20.974892423231157	0.0
28	0.0	0.0	0.0	21.461090581538578	0.0
29	0.0	0.0	0.0	22.022171938335315	0.0
30	0.0	0.0	0.0	22.608214361295154	0.0
31	0.0	0.0	0.0	23.179605723680996	0.0
32	0.0	0.0	0.0	23.719795753362963	0.0
33	0.0	0.0	0.0	24.255644728060037	0.0
34	0.0	0.0	0.0	24.755137367258676	0.0
35	0.0	0.0	0.0	25.299125820052417	0.0
36	0.0	0.0	0.0	25.855866121864267	0.0
37	0.0	0.0	0.0	26.376521404114236	0.0
38	0.0	0.0	0.0	26.885238785155764	0.0
39	0.0	0.0	0.0	27.415390125185173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGCA	35	1.2089185E-7	45.000004	10
GGTACCC	20	7.027043E-4	45.0	37
CGAACTA	20	7.027043E-4	45.0	22
GTCGATG	20	7.027043E-4	45.0	1
CTCCGAT	20	7.027043E-4	45.0	42
TTTCGTC	25	3.885631E-5	45.0	16
TGGCGCG	20	7.027043E-4	45.0	1
TAAACGG	25	3.885631E-5	45.0	2
ACGGGCA	45	3.8380676E-10	45.0	5
CGTTAAC	20	7.027043E-4	45.0	25
CCACTAG	20	7.027043E-4	45.0	42
CACTAGA	20	7.027043E-4	45.0	43
CTTTGCG	20	7.027043E-4	45.0	1
TCCGTTA	20	7.027043E-4	45.0	23
TTACGCG	20	7.027043E-4	45.0	1
GCTACGA	90	0.0	45.0	10
TCGATGG	30	2.1614742E-6	44.999996	2
CGTCATA	30	2.1614742E-6	44.999996	38
CGGGAGT	100	0.0	42.75	6
AATATAC	95	0.0	42.63158	16
>>END_MODULE