##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936269.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186149
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.705676635383483	31.0	31.0	34.0	30.0	34.0
2	31.829217454834566	31.0	31.0	34.0	30.0	34.0
3	31.748035176122354	31.0	31.0	34.0	30.0	34.0
4	35.526997190422726	37.0	35.0	37.0	33.0	37.0
5	30.92699396719832	35.0	35.0	37.0	0.0	37.0
6	33.21589694277165	37.0	35.0	37.0	17.0	37.0
7	29.678612294452293	35.0	32.0	37.0	0.0	37.0
8	32.65982089616383	35.0	33.0	37.0	17.0	37.0
9	36.402306754266746	39.0	35.0	39.0	32.0	39.0
10	36.63877861283166	38.0	35.0	39.0	32.0	39.0
11	36.809657854729274	39.0	37.0	39.0	33.0	39.0
12	36.74706820880048	39.0	35.0	39.0	33.0	39.0
13	36.46244137760611	39.0	35.0	39.0	32.0	39.0
14	37.64437090717651	39.0	36.0	41.0	33.0	41.0
15	37.77788223412428	39.0	36.0	41.0	33.0	41.0
16	37.859322370788995	40.0	36.0	41.0	33.0	41.0
17	37.84704725784184	40.0	36.0	41.0	33.0	41.0
18	37.775572256633126	39.0	36.0	41.0	33.0	41.0
19	37.66073414307893	39.0	36.0	41.0	33.0	41.0
20	37.5492804151513	39.0	36.0	41.0	33.0	41.0
21	37.465626997727625	39.0	36.0	41.0	32.0	41.0
22	37.38973080704167	39.0	35.0	41.0	32.0	41.0
23	37.35083186049885	39.0	35.0	41.0	32.0	41.0
24	37.31713842137213	39.0	35.0	41.0	32.0	41.0
25	37.17788438294055	39.0	35.0	40.0	32.0	41.0
26	37.035074053580736	39.0	35.0	40.0	32.0	41.0
27	37.04014525997991	39.0	35.0	40.0	32.0	41.0
28	36.8017018624865	39.0	35.0	40.0	31.0	41.0
29	36.75621142203289	39.0	35.0	40.0	31.0	41.0
30	36.70840026000677	39.0	35.0	40.0	31.0	41.0
31	36.54040043191207	39.0	35.0	40.0	30.0	41.0
32	36.50339781572826	39.0	35.0	40.0	30.0	41.0
33	36.36883356880778	39.0	35.0	40.0	30.0	41.0
34	36.15591810861192	38.0	35.0	40.0	30.0	41.0
35	36.11484885763555	38.0	35.0	40.0	30.0	41.0
36	36.037233613932926	38.0	35.0	40.0	29.0	41.0
37	35.787326281634606	38.0	35.0	40.0	28.0	41.0
38	35.76677285400405	38.0	35.0	40.0	28.0	41.0
39	35.799510069890246	38.0	35.0	40.0	29.0	41.0
40	35.61880536559423	38.0	35.0	40.0	27.0	41.0
41	35.601888809502064	38.0	35.0	40.0	27.0	41.0
42	35.45752596038657	38.0	34.0	40.0	27.0	41.0
43	35.2375301505783	38.0	34.0	40.0	26.0	41.0
44	34.95331696651607	38.0	34.0	40.0	24.0	41.0
45	34.912833267973504	38.0	34.0	40.0	24.0	41.0
46	34.827299636312844	38.0	34.0	40.0	24.0	41.0
47	34.69428253710737	37.0	33.0	40.0	24.0	41.0
48	34.597172157787575	37.0	33.0	40.0	24.0	41.0
49	34.35569892935229	37.0	33.0	40.0	23.0	41.0
50	34.14749474883024	37.0	33.0	40.0	23.0	41.0
51	31.97655641448517	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	8.0
11	7.0
12	4.0
13	3.0
14	2.0
15	10.0
16	14.0
17	42.0
18	74.0
19	180.0
20	303.0
21	449.0
22	724.0
23	1044.0
24	1317.0
25	1698.0
26	2229.0
27	2595.0
28	3087.0
29	3707.0
30	4415.0
31	5706.0
32	7367.0
33	10389.0
34	14606.0
35	18252.0
36	19758.0
37	28096.0
38	32369.0
39	27679.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39935750393502	21.664365642576648	28.479336445535562	9.456940407952768
2	31.350692187441243	26.092538772703588	25.90236853273453	16.65440050712064
3	30.394468946918867	24.180092291658834	28.840337579036145	16.585101182386154
4	28.636200033306654	25.88249198222929	26.855905752918364	18.6254022315457
5	20.796243869158577	40.07810947144492	22.39281435839032	16.732832301006184
6	24.47770334516973	35.52584220167715	25.905054553073075	14.091399900080043
7	68.85237095015283	19.411331782604258	7.330149503892043	4.406147763350864
8	81.7012178416215	5.816308441087516	8.242859214929975	4.239614502361012
9	76.0079291320394	6.992248145302956	10.238572326469656	6.761250396187999
10	40.34724870936723	27.817501034117832	17.99257583978426	13.842674416730683
11	29.763791371428265	25.801374168005196	25.239458713181378	19.19537574738516
12	28.166146474061097	22.680755738682453	30.58678800315876	18.566309784097683
13	24.062444600830517	27.468318390106848	30.61472261467964	17.854514394382996
14	19.955519503193678	31.29267414812865	28.11672369983185	20.635082648845817
15	18.36754427904528	27.194344315575158	35.762749195536905	18.675362209842653
16	21.694986274436072	26.889212405116332	30.594846064174398	20.820955256273198
17	22.242934423499456	25.950716898828357	27.50699708298191	24.299351594690275
18	23.485487432110837	25.363015648754494	30.1511155042466	21.00038141488807
19	24.379932204846654	29.037491471885424	26.397133479094702	20.185442844173217
20	23.50428957448066	27.81159178937303	28.55508221908256	20.12903641706375
21	22.9987805467663	28.29561265437902	28.752773315999548	19.95283348285513
22	21.216337450107172	25.281360630462697	27.80031050395114	25.701991415478997
23	19.40166210938549	27.928165072065923	29.139023040682467	23.531149777866116
24	21.221709490784264	24.797876969524413	31.343171330493313	22.637242209198007
25	19.599353206302478	28.638886053645194	29.078318981031327	22.683441759021
26	19.17281317654137	29.928175816147277	28.16775808626423	22.73125292104712
27	19.822830098469506	29.180924957963782	29.500561378250755	21.495683565315957
28	18.44060403225373	28.985919881385342	31.71115611687412	20.86231996948681
29	20.15697102858463	26.921981853246592	30.889233893279037	22.03181322488974
30	20.28428839263171	29.227124507786773	29.179850549828362	21.308736549753153
31	21.669200479186028	31.95236074327555	25.624096825661162	20.754341951877258
32	22.990185281682955	31.39366851285798	24.4234457343311	21.192700471127967
33	22.60984480174484	28.23383418659246	25.494630645343246	23.661690366319455
34	19.75997722254753	30.173140871022675	26.999876443064423	23.06700546336537
35	20.922486825070237	28.103830802206836	27.55695706127887	23.416725311444058
36	22.530875803791588	29.028359002734366	27.107317256606265	21.333447936867778
37	21.219560674513428	30.664145388908885	28.882776700385175	19.23351723619251
38	21.74763227307157	30.111362403236118	26.187086688620408	21.953918635071904
39	23.18196713385514	27.228188171840838	26.529822883818877	23.06002181048515
40	22.829561265437903	27.60906585584666	27.518278368403802	22.04309451031163
41	20.981579272518253	27.181451417950136	27.45649990061725	24.380469408914365
42	23.120725870136287	26.45837474281355	27.362489188768137	23.058410198282022
43	22.27946430010368	26.501888272297997	29.08100500136987	22.137642426228453
44	20.61144566986661	27.95663688765451	27.779359545310477	23.652557897168396
45	21.10298739182053	29.063814471203177	25.894847675786604	23.938350461189692
46	21.609033623602595	30.565299840450393	25.29855116062939	22.52711537531762
47	20.392803614308967	28.246727084217483	29.27923330235457	22.081235999118988
48	21.360308140253238	25.53921858296311	29.12989057153141	23.970582705252244
49	22.12367512046801	24.555597934987563	30.183347748309153	23.137379196235276
50	20.55772526309569	26.488458170605266	29.474775583000714	23.479040983298326
51	20.334248370928666	26.332668990969598	26.56903878076165	26.764043857340088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	219.5
2	345.0
3	593.0
4	841.0
5	706.5
6	572.0
7	465.5
8	359.0
9	410.5
10	462.0
11	471.5
12	481.0
13	496.0
14	511.0
15	492.5
16	474.0
17	525.5
18	577.0
19	614.5
20	652.0
21	695.5
22	739.0
23	865.5
24	992.0
25	1088.5
26	1417.0
27	1649.0
28	2011.5
29	2374.0
30	2924.5
31	3475.0
32	4053.0
33	4631.0
34	5430.5
35	6230.0
36	6482.0
37	6734.0
38	7506.5
39	8279.0
40	9305.0
41	10331.0
42	11974.0
43	13617.0
44	14822.0
45	16027.0
46	17815.5
47	19604.0
48	19811.5
49	20019.0
50	18453.0
51	16887.0
52	14212.0
53	11537.0
54	9772.0
55	8007.0
56	7112.0
57	6217.0
58	5784.0
59	5351.0
60	5133.0
61	4915.0
62	4513.0
63	4111.0
64	3468.5
65	2826.0
66	2311.0
67	1796.0
68	1504.0
69	1212.0
70	1022.0
71	832.0
72	665.0
73	498.0
74	393.0
75	249.5
76	211.0
77	167.5
78	124.0
79	84.5
80	45.0
81	30.0
82	15.0
83	11.0
84	7.0
85	6.0
86	5.0
87	4.0
88	3.0
89	4.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	186149.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.04915417219539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87641414249835	39.47697811967832
2	8.634051048665823	8.642539041305621
3	3.6000257604705577	5.405347329289977
4	1.9137882918661315	3.83133941090202
5	1.2526028808792908	3.1345857350831863
6	0.8565356460511345	2.572133076191653
7	0.6193246463302062	2.1697672294774617
8	0.5463366464160745	2.187494963711865
9	0.3960672348281562	1.7840547088622556
>10	3.26728634909731	28.19837871812365
>50	0.020393705858360346	0.6870840026000677
>100	0.016100294098705534	1.4955761245024146
>500	0.0010733529399137025	0.4147215402715029
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	772	0.4147215402715029	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	377	0.2025259335263687	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.17674013827632704	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	271	0.1455823023491934	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	263	0.14128466980751977	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	205	0.11012683388038613	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12087091523457015	0.0
2	0.0	0.0	0.0	0.6167102697301624	0.0
3	0.0	0.0	0.0	0.8976679971420743	0.0
4	0.0	0.0	0.0	1.2549087021686929	0.0
5	0.0	0.0	0.0	2.3228703887745836	0.0
6	0.0	0.0	0.0	3.020161268661126	0.0
7	0.0	0.0	0.0	3.685757108552826	0.0
8	0.0	0.0	0.0	4.929384525299626	0.0
9	0.0	0.0	0.0	5.390305615394119	0.0
10	0.0	0.0	0.0	6.664553664000344	0.0
11	0.0	0.0	0.0	8.228354705101827	0.0
12	0.0	0.0	0.0	9.441898694056912	0.0
13	0.0	0.0	0.0	9.974805129224439	0.0
14	0.0	0.0	0.0	10.187537940037283	0.0
15	0.0	0.0	0.0	10.550150685740993	0.0
16	0.0	0.0	0.0	11.462323192711215	0.0
17	0.0	0.0	0.0	12.580782061681772	0.0
18	0.0	0.0	0.0	13.888873966553675	0.0
19	0.0	0.0	0.0	14.586164846440218	0.0
20	0.0	0.0	0.0	15.248000257857953	0.0
21	0.0	0.0	0.0	16.06884807331761	0.0
22	0.0	0.0	0.0	16.88217503182934	0.0
23	0.0	0.0	0.0	17.815835701507932	0.0
24	0.0	0.0	0.0	18.457257358352717	0.0
25	0.0	0.0	0.0	19.08739772977561	0.0
26	0.0	0.0	0.0	19.66650371476613	0.0
27	0.0	0.0	0.0	20.238626046876426	0.0
28	0.0	0.0	0.0	20.839220194575315	0.0
29	0.0	0.0	0.0	21.443574770748164	0.0
30	0.0	0.0	0.0	22.122600712332595	0.0
31	0.0	0.0	0.0	22.765633981380507	0.0
32	0.0	0.0	0.0	23.391476720261725	0.0
33	0.0	0.0	0.0	23.972194317455372	0.0
34	0.0	0.0	0.0	24.548614282107344	0.0
35	0.0	0.0	0.0	25.176605837259398	0.0
36	0.0	0.0	0.0	25.77397676055203	0.0
37	0.0	0.0	0.0	26.36543843909986	0.0
38	0.0	0.0	0.0	26.93702356714245	0.0
39	0.0	0.0	0.0	27.559643081617413	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	25	3.8781727E-5	45.0	24
CCCTCGT	145	0.0	45.0	14
GGTAACG	25	3.8781727E-5	45.0	1
CGACGGT	25	3.8781727E-5	45.0	28
TATGGGT	35	1.2046075E-7	45.0	4
CCTCGTG	145	0.0	45.0	15
GACCCTC	25	3.8781727E-5	45.0	44
ACGGGCG	20	7.01804E-4	45.0	5
ACCTACA	20	7.01804E-4	45.0	25
TCGCTTC	20	7.01804E-4	45.0	12
GCCCTCG	145	0.0	45.0	13
GAAGTCC	20	7.01804E-4	45.0	9
CTACCGC	20	7.01804E-4	45.0	45
CTTCTCG	20	7.01804E-4	45.0	15
CTATTAG	20	7.01804E-4	45.0	19
CGATGTG	25	3.8781727E-5	45.0	10
GCGGATG	25	3.8781727E-5	45.0	9
CGGTCTA	25	3.8781727E-5	45.0	31
ACGTAGG	20	7.01804E-4	45.0	2
TTGTTAC	20	7.01804E-4	45.0	36
>>END_MODULE