##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936268.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452085
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.936644657531218	31.0	31.0	34.0	30.0	34.0
2	32.07021246004623	33.0	31.0	34.0	30.0	34.0
3	32.00904697125541	33.0	31.0	34.0	30.0	34.0
4	35.72995343795968	37.0	35.0	37.0	35.0	37.0
5	31.239050178616854	37.0	35.0	37.0	0.0	37.0
6	33.442848136965395	37.0	35.0	37.0	17.0	37.0
7	29.53841423626088	35.0	32.0	37.0	0.0	37.0
8	32.56705929194731	35.0	33.0	37.0	17.0	37.0
9	36.43377462202904	39.0	35.0	39.0	32.0	39.0
10	36.85276883771857	38.0	37.0	39.0	33.0	39.0
11	36.991813486401895	39.0	37.0	39.0	33.0	39.0
12	37.027598792262516	39.0	37.0	39.0	33.0	39.0
13	36.85051704878507	39.0	37.0	39.0	33.0	39.0
14	38.12796708583563	40.0	37.0	41.0	33.0	41.0
15	38.20443943063805	40.0	37.0	41.0	33.0	41.0
16	38.25254100445713	40.0	37.0	41.0	34.0	41.0
17	38.1973633276928	40.0	37.0	41.0	33.0	41.0
18	38.10349602397779	40.0	37.0	41.0	33.0	41.0
19	38.006140438191935	40.0	37.0	41.0	33.0	41.0
20	37.956010484753975	40.0	37.0	41.0	33.0	41.0
21	37.87260360330469	40.0	37.0	41.0	33.0	41.0
22	37.85308736188991	40.0	36.0	41.0	33.0	41.0
23	37.82968468319011	40.0	36.0	41.0	33.0	41.0
24	37.760224294103985	40.0	36.0	41.0	33.0	41.0
25	37.638581240253494	40.0	36.0	41.0	33.0	41.0
26	37.45949987281153	39.0	36.0	41.0	32.0	41.0
27	37.41823550880918	39.0	36.0	41.0	32.0	41.0
28	37.318404724775206	39.0	36.0	41.0	32.0	41.0
29	37.2804981364124	39.0	36.0	41.0	32.0	41.0
30	37.16648417886017	39.0	36.0	41.0	31.0	41.0
31	37.01301525155667	39.0	35.0	41.0	31.0	41.0
32	36.87652985611113	39.0	35.0	41.0	31.0	41.0
33	36.74617162701704	39.0	35.0	41.0	30.0	41.0
34	36.59323578530586	39.0	35.0	41.0	30.0	41.0
35	36.43434309919595	39.0	35.0	41.0	30.0	41.0
36	36.313954234270106	39.0	35.0	41.0	30.0	41.0
37	36.18417111826316	39.0	35.0	40.0	29.0	41.0
38	36.042169061127886	39.0	35.0	40.0	29.0	41.0
39	36.012800690135705	39.0	35.0	40.0	29.0	41.0
40	35.86456529192519	39.0	35.0	40.0	28.0	41.0
41	35.73903580078967	39.0	35.0	40.0	27.0	41.0
42	35.71089728701461	38.0	35.0	40.0	27.0	41.0
43	35.56280124312906	38.0	35.0	40.0	26.0	41.0
44	35.42686662906312	38.0	34.0	40.0	26.0	41.0
45	35.281424953272065	38.0	34.0	40.0	26.0	41.0
46	35.127515843259566	38.0	34.0	40.0	25.0	41.0
47	34.94233385314708	38.0	34.0	40.0	24.0	41.0
48	34.82550184146787	38.0	34.0	40.0	23.0	41.0
49	34.667476248935486	38.0	34.0	40.0	23.0	41.0
50	34.50223741110632	38.0	33.0	40.0	23.0	41.0
51	32.363062255991686	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	22.0
10	15.0
11	20.0
12	16.0
13	5.0
14	16.0
15	34.0
16	63.0
17	112.0
18	192.0
19	355.0
20	564.0
21	1071.0
22	1490.0
23	2189.0
24	2979.0
25	4014.0
26	4998.0
27	5915.0
28	6769.0
29	8144.0
30	9957.0
31	12195.0
32	15888.0
33	22055.0
34	31090.0
35	38188.0
36	44526.0
37	69738.0
38	86072.0
39	83377.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51128659433513	20.75096497340102	24.733623101850316	11.004125330413528
2	31.55424311799772	26.259221164161605	24.996847937887786	17.189687779952887
3	32.30454449937511	25.2501188935709	25.873674198436134	16.571662408617847
4	28.438678567083624	25.629029939060132	27.256378778327083	18.675912715529158
5	22.80013714235155	39.574195118174686	20.704734729088557	16.920933010385212
6	25.370007852505612	35.80543481867348	24.63342070628311	14.191136622537798
7	68.67314774876407	19.70647112821704	7.344194122786645	4.276187000232257
8	81.55612329539798	6.780583297388764	7.829279892055697	3.834013515157548
9	75.46213654511872	8.021500381565414	9.802802570313105	6.713560503002753
10	40.11966776159351	28.234292223807472	16.592676156032603	15.05336385856642
11	30.731610206045325	26.7759381532234	24.834931484123558	17.657520156607717
12	29.472776137230834	23.416835329639337	27.898735857194996	19.211652675934836
13	24.417310903922935	25.047059734341993	29.507946514482896	21.027682847252176
14	20.07896745081124	29.179468462789078	28.226992711547606	22.514571374852075
15	19.034916000309675	27.47691252751142	33.84120242874681	19.646969043432097
16	21.890573675304424	26.289967594589513	30.972051715938377	20.84740701416769
17	22.24979815742615	25.68123251158521	28.861165488790824	23.207803842197816
18	23.11622814293772	26.63724742028601	29.445126469579836	20.801397967196433
19	24.446730150303594	28.154218786290187	26.992047955583576	20.407003107822643
20	26.302133448355953	26.58792041319663	28.688631562648613	18.4213145757988
21	24.595153566254133	27.833482641538648	27.794109514803633	19.777254277403586
22	23.5575168386476	24.735613877921185	28.00380459426878	23.70306468916244
23	22.75125252994459	27.351936029728925	28.195582689096078	21.70122875123041
24	23.302255106893615	25.306966610261345	28.99830784033976	22.39247044250528
25	21.590187685944016	27.918201223221295	27.958459139321146	22.533151951513542
26	22.005153898050146	27.973942953205704	27.971952177134828	22.048950971609322
27	22.344470619463156	28.01950960549454	27.871307386885206	21.764712388157097
28	20.297731621265914	27.871528584226418	29.75679352334185	22.07394627116582
29	21.70874946083148	26.25877876947919	29.723835119501864	22.308636650187466
30	22.196931992877445	26.61070373934105	28.314586858665958	22.877777409115545
31	23.484521716048974	27.26832343475231	26.39415154229846	22.853003306900252
32	23.537387880597674	27.253282015550173	27.798312264286583	21.41101783956557
33	22.687990090359115	27.274959354988553	26.959531946426	23.07751860822633
34	23.069555503942844	26.13756262649723	28.4769457071126	22.31593616244733
35	21.90826946260106	25.82899233551213	28.285831204308927	23.976906997577892
36	22.684229735558578	27.324065164736723	27.93103066901136	22.060674430693343
37	22.97775860734154	27.58618401406815	28.794806286428436	20.641251092161873
38	22.633575544421955	26.653394826194187	27.06526427552341	23.647765353860446
39	22.77845980291317	25.241049802581372	29.052501188935707	22.927989205569748
40	23.084154528462566	25.767499474656315	29.260647887012396	21.88769810986872
41	20.924383688908062	26.57796653284227	29.609697291438557	22.88795248681111
42	21.50502670957895	26.0561620049327	29.626065894687947	22.8127453908004
43	22.642202240729066	25.44388776446907	28.770032184213147	23.143877810588716
44	22.482497760376923	25.830098322218163	27.921076788657	23.766327128747914
45	21.802758330844863	25.89358195914485	27.450147649225254	24.85351206078503
46	22.05027815565657	27.120121216142984	27.479345698264705	23.350254929935744
47	21.35770928033445	25.879204131966333	30.507316102060457	22.255770485638763
48	21.88858289923355	25.685877655750577	29.382970016700398	23.04256942831547
49	21.072143512834977	24.979594545273564	30.122211531017395	23.82605041087406
50	20.226506077396948	25.48834843005187	29.968479378877866	24.316666113673314
51	20.77352710220423	24.716148511894886	28.614972848026365	25.895351537874518
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	501.5
2	840.0
3	1369.0
4	1898.0
5	1493.5
6	1089.0
7	977.0
8	865.0
9	842.0
10	819.0
11	846.5
12	874.0
13	953.0
14	1032.0
15	986.5
16	941.0
17	1025.0
18	1109.0
19	1269.5
20	1430.0
21	1643.5
22	1857.0
23	2014.5
24	2172.0
25	2843.0
26	4228.0
27	4942.0
28	5962.0
29	6982.0
30	8307.5
31	9633.0
32	11089.0
33	12545.0
34	14079.0
35	15613.0
36	15872.5
37	16132.0
38	17726.0
39	19320.0
40	20938.5
41	22557.0
42	24916.5
43	27276.0
44	30151.0
45	33026.0
46	36483.0
47	39940.0
48	38602.0
49	37264.0
50	36110.5
51	34957.0
52	31922.0
53	28887.0
54	26443.5
55	24000.0
56	22397.0
57	20794.0
58	20190.5
59	19587.0
60	18715.5
61	17844.0
62	16041.0
63	14238.0
64	12270.5
65	10303.0
66	8165.5
67	6028.0
68	5159.0
69	4290.0
70	3461.0
71	2632.0
72	2181.5
73	1731.0
74	1452.0
75	999.0
76	825.0
77	626.0
78	427.0
79	338.5
80	250.0
81	183.5
82	117.0
83	95.0
84	73.0
85	60.0
86	47.0
87	35.5
88	24.0
89	20.0
90	16.0
91	11.0
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.57274128486225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6364716423661	27.291513568638553
2	9.061442141114927	6.809264424717189
3	4.14753941936415	4.67503276717611
4	2.6317023191560587	3.955210815056904
5	1.8577048674830527	3.4899532184785023
6	1.351433995177513	3.046624791862349
7	1.0093509350916718	2.654685708488323
8	0.7768192783912051	2.334978381766891
9	0.628660460914628	2.125844716857082
>10	5.827199642144627	40.29452219850794
>50	0.0528752204233641	1.2944239061292815
>100	0.01586256612700922	1.0502846086908455
>500	0.0023500097965939585	0.6845171440837142
>1k	5.875024491484896E-4	0.29314374954633104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG	1328	0.2937500691241691	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC	998	0.22075494652554276	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	804	0.17784266233119878	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	671	0.14842341595054026	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.13891193027859805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.211973412079587E-4	0.0	0.0	0.0663592023623876	0.0
2	2.211973412079587E-4	0.0	0.0	0.337325945342137	0.0
3	2.211973412079587E-4	0.0	0.0	0.45832089098289036	0.0
4	2.211973412079587E-4	0.0	0.0	0.6826149949677605	0.0
5	2.211973412079587E-4	0.0	0.0	1.4862249355762744	0.0
6	2.211973412079587E-4	0.0	0.0	1.8602696395589324	0.0
7	2.211973412079587E-4	0.0	0.0	2.2947012176913635	0.0
8	2.211973412079587E-4	0.0	0.0	2.9470121769136335	0.0
9	2.211973412079587E-4	0.0	0.0	3.1991771458907063	0.0
10	2.211973412079587E-4	0.0	0.0	4.3286107700985434	0.0
11	2.211973412079587E-4	0.0	0.0	5.158543194310805	0.0
12	2.211973412079587E-4	0.0	0.0	6.189101606998684	0.0
13	2.211973412079587E-4	0.0	0.0	6.501211055443114	0.0
14	2.211973412079587E-4	0.0	0.0	6.639901788380504	0.0
15	2.211973412079587E-4	0.0	0.0	6.911310926042669	0.0
16	2.211973412079587E-4	0.0	0.0	7.431124677881372	0.0
17	2.211973412079587E-4	0.0	0.0	8.001592620856698	0.0
18	2.211973412079587E-4	0.0	0.0	8.637534976829578	0.0
19	2.211973412079587E-4	0.0	0.0	9.010916088788612	0.0
20	2.211973412079587E-4	0.0	0.0	9.37522810975812	0.0
21	2.211973412079587E-4	0.0	0.0	9.825364699116317	0.0
22	2.211973412079587E-4	0.0	0.0	10.29629383854806	0.0
23	2.211973412079587E-4	0.0	0.0	10.76324142583806	0.0
24	2.211973412079587E-4	0.0	0.0	11.15785748255306	0.0
25	2.211973412079587E-4	0.0	0.0	11.510446044438545	0.0
26	2.211973412079587E-4	0.0	0.0	11.824988663636264	0.0
27	2.211973412079587E-4	0.0	0.0	12.158775451519073	0.0
28	2.211973412079587E-4	0.0	0.0	12.477963214882157	0.0
29	2.211973412079587E-4	0.0	0.0	12.817279936295165	0.0
30	2.211973412079587E-4	0.0	0.0	13.179158786511387	0.0
31	4.423946824159174E-4	0.0	0.0	13.55806983200062	0.0
32	4.423946824159174E-4	0.0	0.0	13.924593826382207	0.0
33	4.423946824159174E-4	0.0	0.0	14.271431257396285	0.0
34	4.423946824159174E-4	0.0	0.0	14.624019819281772	0.0
35	4.423946824159174E-4	0.0	0.0	15.018193481314354	0.0
36	4.423946824159174E-4	0.0	0.0	15.377860358118495	0.0
37	4.423946824159174E-4	0.0	0.0	15.737748432263844	0.0
38	4.423946824159174E-4	0.0	0.0	16.107147992081135	0.0
39	4.423946824159174E-4	0.0	0.0	16.509284758397204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	7.0287404E-4	45.0	1
GCGCGAC	20	7.0287404E-4	45.0	9
ACTCTTA	20	7.0287404E-4	45.0	5
GGTCCGA	20	7.0287404E-4	45.0	11
CTAAGCG	20	7.0287404E-4	45.0	1
CGTTCGG	20	7.0287404E-4	45.0	2
CTAACGG	35	1.2097371E-7	45.0	2
GCGATCA	25	3.8870377E-5	45.0	9
ATCGATG	20	7.0287404E-4	45.0	2
TTTACGG	30	2.1625692E-6	44.999996	2
CGAATAT	120	0.0	44.999996	14
GCTACGA	135	0.0	40.000004	10
TATTGCG	40	3.4520235E-7	39.375	1
TACGGCT	185	0.0	38.918922	7
CATGCGG	35	6.2398813E-6	38.571426	2
GCGCGTC	35	6.2398813E-6	38.571426	22
AGCGCGT	35	6.2398813E-6	38.571426	21
CACAACG	90	0.0	37.5	12
CGCATGG	30	1.13882656E-4	37.499996	2
ATACTCG	30	1.13882656E-4	37.499996	44
>>END_MODULE