FastQCFastQC Report
Sat 14 Jan 2017
SRR2936267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741487
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38530.5196314972480974No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.17532337047041957No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.10060864182379463No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.031665E-445.01
CGATTGT253.889462E-545.010
TAACGCG207.031665E-445.01
CGTACGT207.031665E-445.021
GTATACG207.031665E-445.01
TAGTACG207.031665E-445.01
TATACGG750.042.0000042
CGTTATT3300.041.5909081
CGTTTTA2850.040.2631571
CGTTTTT17350.039.5533141
ATCGTTG403.456007E-739.37523
CCCCGAT403.456007E-739.37540
TATCGCG356.2452873E-638.5714332
TGTAACG659.094947E-1238.076921
GACCGAC301.1395314E-437.4999969
GCGATAT601.546141E-1037.4999969
TTACGGG1950.036.9230773
ACGGGAC2200.036.818185
CGGTTTT5450.036.743121
TACGGGA2550.036.176474
Produced by FastQC (version 0.11.5)