##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936267.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741487
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.887836199420892	31.0	31.0	34.0	30.0	34.0
2	32.01597870225641	33.0	31.0	34.0	30.0	34.0
3	31.793280259802263	33.0	31.0	34.0	30.0	34.0
4	35.596773780255084	37.0	35.0	37.0	33.0	37.0
5	31.129322563982914	37.0	35.0	37.0	0.0	37.0
6	33.37798100303849	37.0	35.0	37.0	17.0	37.0
7	29.597371228355993	35.0	32.0	37.0	0.0	37.0
8	32.66032985069192	35.0	35.0	37.0	17.0	37.0
9	36.53099919486114	39.0	35.0	39.0	32.0	39.0
10	36.845556294311294	38.0	37.0	39.0	33.0	39.0
11	36.98898160048659	39.0	37.0	39.0	33.0	39.0
12	37.0094553242336	39.0	37.0	39.0	33.0	39.0
13	36.85241683266194	39.0	37.0	39.0	33.0	39.0
14	38.03177264065317	40.0	37.0	41.0	33.0	41.0
15	38.14599311923203	40.0	37.0	41.0	33.0	41.0
16	38.20287206653657	40.0	37.0	41.0	33.0	41.0
17	38.1961153735669	40.0	37.0	41.0	33.0	41.0
18	38.08692262979661	40.0	37.0	41.0	33.0	41.0
19	37.98201856539629	40.0	37.0	41.0	33.0	41.0
20	37.85316937451365	40.0	36.0	41.0	33.0	41.0
21	37.76169103436742	40.0	36.0	41.0	33.0	41.0
22	37.742988076662165	40.0	36.0	41.0	33.0	41.0
23	37.698811981868865	39.0	36.0	41.0	33.0	41.0
24	37.639203384550235	39.0	36.0	41.0	33.0	41.0
25	37.48498355331921	39.0	35.0	41.0	33.0	41.0
26	37.337482653101134	39.0	35.0	41.0	32.0	41.0
27	37.29709624039262	39.0	35.0	41.0	32.0	41.0
28	37.159625185606764	39.0	35.0	41.0	32.0	41.0
29	37.11280440520198	39.0	35.0	41.0	32.0	41.0
30	36.971860599039495	39.0	35.0	41.0	31.0	41.0
31	36.82785537710034	39.0	35.0	41.0	31.0	41.0
32	36.691206993514385	39.0	35.0	41.0	31.0	41.0
33	36.49802761208221	39.0	35.0	41.0	30.0	41.0
34	36.2827750183078	39.0	35.0	41.0	30.0	41.0
35	36.11136540492281	39.0	35.0	41.0	29.0	41.0
36	35.94759314728377	39.0	35.0	40.0	28.0	41.0
37	35.834775255668674	39.0	35.0	40.0	27.0	41.0
38	35.67867406980837	39.0	35.0	40.0	27.0	41.0
39	35.65389143707172	39.0	35.0	40.0	26.0	41.0
40	35.542631226171196	39.0	35.0	40.0	26.0	41.0
41	35.422348604898	38.0	35.0	40.0	25.0	41.0
42	35.37392698725669	38.0	35.0	40.0	25.0	41.0
43	35.26019067090859	38.0	34.0	40.0	25.0	41.0
44	35.0180286370496	38.0	34.0	40.0	24.0	41.0
45	34.94446564808284	38.0	34.0	40.0	24.0	41.0
46	34.90535774733745	38.0	34.0	40.0	23.0	41.0
47	34.79158232039132	38.0	34.0	40.0	23.0	41.0
48	34.68732560382043	38.0	34.0	40.0	23.0	41.0
49	34.55684995151635	38.0	34.0	40.0	23.0	41.0
50	34.40357956376848	37.0	33.0	40.0	23.0	41.0
51	32.47713715817	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	33.0
10	59.0
11	37.0
12	21.0
13	22.0
14	31.0
15	70.0
16	124.0
17	196.0
18	389.0
19	713.0
20	1260.0
21	1940.0
22	2906.0
23	4125.0
24	6041.0
25	8349.0
26	10449.0
27	11443.0
28	12366.0
29	13586.0
30	15705.0
31	19937.0
32	25628.0
33	35517.0
34	49793.0
35	60084.0
36	72484.0
37	113332.0
38	140495.0
39	134325.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.894967814675105	20.608992470535558	25.645493447626187	13.850546267163146
2	32.84076457173221	24.384378957419347	25.709553909913456	17.065302560934985
3	32.14028027463732	23.38813762075397	28.473189685051793	15.998392419556918
4	27.76865946402297	25.236989994430107	28.63772392503173	18.356626616515193
5	21.53051907855431	38.65947750938317	23.33122495741665	16.478778454645866
6	25.153509097259963	34.39318558518221	26.494598017227545	13.958707300330284
7	68.84247464891496	19.061966022330804	8.20810074890052	3.887458579853726
8	83.4191293980879	4.45105578385056	8.914384203634048	3.2154306144274956
9	77.43358953022778	6.866607236539549	10.867351686543392	4.8324515466892874
10	40.209201240210554	27.908648432137046	18.520216807577206	13.361933520075201
11	29.619265071403817	25.690672931555103	26.799390953583814	17.890671043457267
12	26.14961556979421	23.544849741128303	30.959409942453476	19.346124746624014
13	24.3269268375575	23.510998844214395	32.49834454279036	19.663729775437737
14	20.631919372827845	26.2727465215169	32.6004366900566	20.494897415598658
15	20.37945371935044	25.703754752274822	34.56244006975172	19.354351458623007
16	24.23535409251949	25.48527485984245	31.175866872918878	19.10350417471918
17	24.77980058989571	24.818911187923725	29.768020208041406	20.633268014139155
18	25.159982575554256	24.7641563506845	30.51934828257272	19.556512791188517
19	24.834555427134934	26.760415219686926	28.666854577356045	19.7381747758221
20	25.05451882500974	26.739241551099347	29.10718596549906	19.099053658391853
21	25.322493853567224	26.017988177810263	30.127163389243506	18.532354579379003
22	23.65355023081996	23.971559852027074	31.047206491819818	21.32768342533315
23	22.623323133109547	24.82538466621802	31.562792065133983	20.98850013553845
24	22.207537016832394	25.51737252305165	30.79069491440848	21.48439554570748
25	22.888735743175538	26.544902338139444	29.203209226864395	21.363152691820623
26	21.921625058834476	27.195082314322438	28.755999768033696	22.12729285880939
27	21.16881347886072	26.870059758296506	30.39581273845664	21.565314024386133
28	20.72564994396395	26.777408100209442	30.615506408069194	21.881435547757413
29	22.00200408098861	26.225544075621016	30.6207661091833	21.151685734207074
30	23.390430310983202	25.40111964201665	30.250294340966192	20.958155706033956
31	23.503041860477662	25.91710980772421	29.853658931309653	20.72618940048848
32	24.06475096663866	26.344224511016378	28.9360433830937	20.654981139251262
33	24.148501592071067	25.5648446972098	28.928490991750362	21.358162718968778
34	22.154940005691266	26.035385650726177	30.36883991223042	21.440834431352133
35	22.198635984177738	27.138169650985116	29.16639131906561	21.496803045771536
36	23.282808734340588	27.18955288494606	28.577439658416125	20.950198722297223
37	22.66297318766209	27.758949246581533	28.365568108409185	21.212509457347196
38	22.509228078172644	27.227045113400507	28.450937103415164	21.81278970501169
39	22.651240008253684	26.2727465215169	28.043782291530402	23.03223117869902
40	23.13607655966996	25.62728678992349	29.14589197113368	22.090744679272866
41	21.4528373390228	26.747603127229475	28.737523382068737	23.062036151678992
42	21.849472748679343	26.589946957937226	29.488716592468915	22.071863700914513
43	22.30248136514868	25.7127906490606	29.679953930412807	22.30477405537791
44	22.134036065365947	25.8233792365881	29.234902297680204	22.80768240036575
45	22.127562587071655	25.80706067672124	28.28950473845125	23.77587199775586
46	22.071998565045643	26.240513994176567	28.6671243056183	23.02036313515948
47	21.211430544298146	26.480707011721044	29.758849447124497	22.549012996856316
48	21.496533317509275	25.830527035538047	29.86404346940675	22.808896177545932
49	22.23437497892748	25.603146110451025	29.805512436495853	22.35696647412564
50	20.96705673868861	26.005445813615076	30.038962247483774	22.988535200212546
51	21.315006197006824	25.504695294725327	29.120402650349902	24.05989585791794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	240.0
1	668.0
2	1096.0
3	4939.5
4	8783.0
5	6890.5
6	4998.0
7	4101.5
8	3205.0
9	2995.5
10	2786.0
11	2687.5
12	2589.0
13	2526.5
14	2464.0
15	2437.5
16	2411.0
17	2287.0
18	2163.0
19	2262.5
20	2362.0
21	2914.0
22	3466.0
23	3916.0
24	4366.0
25	4865.0
26	6364.0
27	7364.0
28	8886.5
29	10409.0
30	12878.5
31	15348.0
32	17526.5
33	19705.0
34	21763.5
35	23822.0
36	26034.0
37	28246.0
38	30729.5
39	33213.0
40	35297.0
41	37381.0
42	40615.5
43	43850.0
44	46532.5
45	49215.0
46	52604.0
47	55993.0
48	57276.0
49	58559.0
50	57349.5
51	56140.0
52	51971.5
53	47803.0
54	44339.0
55	40875.0
56	37876.0
57	34877.0
58	33447.0
59	32017.0
60	29346.0
61	26675.0
62	24236.0
63	21797.0
64	19276.5
65	16756.0
66	14441.0
67	12126.0
68	10157.0
69	8188.0
70	6801.0
71	5414.0
72	4651.5
73	3889.0
74	3049.0
75	1871.5
76	1534.0
77	1137.0
78	740.0
79	584.5
80	429.0
81	346.5
82	264.0
83	223.5
84	183.0
85	125.5
86	68.0
87	60.5
88	53.0
89	39.0
90	25.0
91	19.0
92	13.0
93	10.5
94	8.0
95	6.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	741487.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.223087962849526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34903285117353	29.161986554743685
2	8.391688857121338	6.582959003994689
3	3.934685792389906	4.629915808232507
4	2.4791505466674524	3.8895975986033586
5	1.6936274515292196	3.321464925381363
6	1.227477379486217	2.888727193679755
7	0.9604138699972703	2.636927839255056
8	0.807829700735605	2.5348460328764038
9	0.6326916104055784	2.233450681946543
>10	5.476007274076704	39.69264896933725
>50	0.036783921465078	0.9321184378428018
>100	0.009195978958472118	0.603608693507566
>500	7.073829968055291E-4	0.17687912760793698
>1k	7.073829968055291E-4	0.7148691329911366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3853	0.5196314972480974	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1300	0.17532337047041957	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	746	0.10060864182379463	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.062172364451433405	0.0
2	0.0	0.0	0.0	0.19771081623818085	0.0
3	0.0	0.0	0.0	0.32353905058348964	0.0
4	0.0	0.0	0.0	0.4542223936495178	0.0
5	1.34864131131092E-4	0.0	0.0	0.8433054119627181	0.0
6	1.34864131131092E-4	0.0	0.0	1.2394013650947353	0.0
7	1.34864131131092E-4	0.0	0.0	1.5843838125280687	0.0
8	1.34864131131092E-4	0.0	0.0	2.1916769950113757	0.0
9	1.34864131131092E-4	0.0	0.0	2.520340882577847	0.0
10	1.34864131131092E-4	0.0	0.0	3.130735940077169	0.0
11	1.34864131131092E-4	0.0	0.0	3.8262302643202104	0.0
12	1.34864131131092E-4	0.0	0.0	4.380386979137867	0.0
13	1.34864131131092E-4	0.0	0.0	4.657263040349999	0.0
14	1.34864131131092E-4	0.0	0.0	4.814784345511115	0.0
15	2.69728262262184E-4	0.0	0.0	4.990647172506058	0.0
16	2.69728262262184E-4	0.0	0.0	5.340754456922373	0.0
17	2.69728262262184E-4	0.0	0.0	5.760047040608939	0.0
18	2.69728262262184E-4	0.0	0.0	6.227755847371566	0.0
19	2.69728262262184E-4	0.0	0.0	6.508812696648762	0.0
20	2.69728262262184E-4	0.0	0.0	6.8105037579890135	0.0
21	2.69728262262184E-4	0.0	0.0	7.170456123977899	0.0
22	2.69728262262184E-4	0.0	0.0	7.5577859085863945	0.0
23	2.69728262262184E-4	0.0	0.0	7.9372935735892876	0.0
24	2.69728262262184E-4	0.0	0.0	8.24775080345306	0.0
25	2.69728262262184E-4	0.0	0.0	8.526784690763291	0.0
26	2.69728262262184E-4	0.0	0.0	8.784510045354807	0.0
27	2.69728262262184E-4	0.0	0.0	9.084582737121487	0.0
28	2.69728262262184E-4	0.0	0.0	9.388296760428705	0.0
29	4.0459239339327597E-4	0.0	0.0	9.688639180457647	0.0
30	4.0459239339327597E-4	0.0	0.0	10.053176926904989	0.0
31	4.0459239339327597E-4	0.0	0.0	10.367005760047041	0.0
32	4.0459239339327597E-4	0.0	0.0	10.679890544271174	0.0
33	4.0459239339327597E-4	0.0	0.0	10.997090980691503	0.0
34	4.0459239339327597E-4	0.0	0.0	11.325889732389104	0.0
35	4.0459239339327597E-4	0.0	0.0	11.678019978772387	0.0
36	4.0459239339327597E-4	0.0	0.0	12.011404110928446	0.0
37	4.0459239339327597E-4	0.0	0.0	12.348429574625044	0.0
38	4.0459239339327597E-4	0.0	0.0	12.736433679889196	0.0
39	4.0459239339327597E-4	0.0	0.0	13.26442675326742	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.031665E-4	45.0	1
CGATTGT	25	3.889462E-5	45.0	10
TAACGCG	20	7.031665E-4	45.0	1
CGTACGT	20	7.031665E-4	45.0	21
GTATACG	20	7.031665E-4	45.0	1
TAGTACG	20	7.031665E-4	45.0	1
TATACGG	75	0.0	42.000004	2
CGTTATT	330	0.0	41.590908	1
CGTTTTA	285	0.0	40.263157	1
CGTTTTT	1735	0.0	39.553314	1
ATCGTTG	40	3.456007E-7	39.375	23
CCCCGAT	40	3.456007E-7	39.375	40
TATCGCG	35	6.2452873E-6	38.57143	32
TGTAACG	65	9.094947E-12	38.07692	1
GACCGAC	30	1.1395314E-4	37.499996	9
GCGATAT	60	1.546141E-10	37.499996	9
TTACGGG	195	0.0	36.923077	3
ACGGGAC	220	0.0	36.81818	5
CGGTTTT	545	0.0	36.74312	1
TACGGGA	255	0.0	36.17647	4
>>END_MODULE