##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936266.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	232374
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89843528105554	31.0	31.0	34.0	30.0	34.0
2	32.018392763390054	33.0	31.0	34.0	30.0	34.0
3	31.97027636482567	33.0	31.0	34.0	30.0	34.0
4	35.71009235112362	37.0	35.0	37.0	35.0	37.0
5	31.297008271149096	37.0	35.0	37.0	0.0	37.0
6	33.444714124643895	37.0	35.0	37.0	17.0	37.0
7	29.32510091490442	35.0	32.0	37.0	0.0	37.0
8	32.340300549975474	35.0	33.0	37.0	17.0	37.0
9	36.260670298742546	39.0	35.0	39.0	32.0	39.0
10	36.77688123456153	38.0	35.0	39.0	33.0	39.0
11	36.9285591331216	39.0	37.0	39.0	33.0	39.0
12	36.918183617788564	39.0	35.0	39.0	33.0	39.0
13	36.68227082203689	39.0	35.0	39.0	33.0	39.0
14	37.99382030691902	40.0	37.0	41.0	33.0	41.0
15	38.03936326783547	40.0	37.0	41.0	33.0	41.0
16	38.11695800735022	40.0	37.0	41.0	33.0	41.0
17	38.05068553280488	40.0	37.0	41.0	33.0	41.0
18	37.954431218638916	40.0	37.0	41.0	33.0	41.0
19	37.85452331155809	40.0	36.0	41.0	33.0	41.0
20	37.80918691419866	40.0	36.0	41.0	33.0	41.0
21	37.74682193360703	40.0	36.0	41.0	33.0	41.0
22	37.743353387212	40.0	36.0	41.0	33.0	41.0
23	37.71748560510212	40.0	36.0	41.0	33.0	41.0
24	37.63058259529896	40.0	36.0	41.0	33.0	41.0
25	37.4948143940372	39.0	36.0	41.0	33.0	41.0
26	37.282238976821844	39.0	35.0	41.0	32.0	41.0
27	37.21572981486741	39.0	35.0	41.0	32.0	41.0
28	37.12094296263782	39.0	35.0	41.0	32.0	41.0
29	37.04997116717016	39.0	35.0	41.0	31.0	41.0
30	36.9277543959307	39.0	35.0	41.0	31.0	41.0
31	36.81981202716311	39.0	35.0	41.0	31.0	41.0
32	36.62219095079484	39.0	35.0	41.0	30.0	41.0
33	36.48591494745539	39.0	35.0	40.0	30.0	41.0
34	36.36504944615146	39.0	35.0	40.0	30.0	41.0
35	36.16931326224104	39.0	35.0	40.0	29.0	41.0
36	36.09917202440893	39.0	35.0	40.0	29.0	41.0
37	35.851011731088676	38.0	35.0	40.0	28.0	41.0
38	35.8333634571854	38.0	35.0	40.0	28.0	41.0
39	35.77869727250037	38.0	35.0	40.0	28.0	41.0
40	35.60166369731554	38.0	35.0	40.0	27.0	41.0
41	35.51364610498593	38.0	34.0	40.0	27.0	41.0
42	35.43290557463399	38.0	34.0	40.0	26.0	41.0
43	35.2334813705492	38.0	34.0	40.0	26.0	41.0
44	35.08123111879986	38.0	34.0	40.0	25.0	41.0
45	35.0045745221066	38.0	34.0	40.0	25.0	41.0
46	34.83435324089614	38.0	34.0	40.0	24.0	41.0
47	34.62275469716922	37.0	33.0	40.0	23.0	41.0
48	34.556202501140405	37.0	33.0	40.0	23.0	41.0
49	34.36393056021758	37.0	33.0	40.0	23.0	41.0
50	34.24449809359051	37.0	33.0	40.0	23.0	41.0
51	32.19107559365506	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	13.0
10	15.0
11	14.0
12	12.0
13	10.0
14	13.0
15	16.0
16	38.0
17	76.0
18	171.0
19	241.0
20	433.0
21	640.0
22	871.0
23	1217.0
24	1648.0
25	2168.0
26	2869.0
27	3196.0
28	3593.0
29	4327.0
30	5148.0
31	6559.0
32	8580.0
33	12094.0
34	17072.0
35	20825.0
36	23608.0
37	35053.0
38	42379.0
39	39462.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.498093590504965	19.870553504264677	24.668422456901375	10.962930448328986
2	30.76204738912271	27.437665143260432	25.76794305731278	16.032344410304077
3	31.59389604688993	26.176766763923677	26.0971537263205	16.1321834628659
4	28.351708883093636	24.709735168306267	28.730839078382264	18.207716870217837
5	23.44883678896951	39.17348756745591	20.48421940492482	16.89345623864976
6	25.120280237892363	37.28988613183919	23.379551929217556	14.210281701050892
7	67.5045400948471	20.057321387074285	8.345167703787858	4.092970814290756
8	80.18453011094184	7.850706189160578	8.073192353705664	3.8915713461919146
9	74.29101362458795	8.901598285522477	8.775078106845	8.032309983044575
10	41.94746400199678	27.30253814970694	15.883876853692755	14.866120994603527
11	32.76442287002849	24.146849475414633	24.291443965331748	18.79728368922513
12	32.07889006515359	21.722740065583928	26.694466678716207	19.503903190546275
13	24.470896055496745	24.92404485871913	29.320405897389552	21.28465318839457
14	20.44462805649513	29.313520445488738	26.85326241317875	23.388589084837374
15	19.145429350960093	26.403125995162966	34.453940630190985	19.997504023685956
16	21.696058939468273	24.75793333161197	30.79001953746977	22.75598819144999
17	21.42666563384888	24.900806458553884	28.99980204325785	24.672725864339384
18	22.604508249632058	27.2392780603682	28.356872972019243	21.7993407179805
19	25.313072891115183	27.538795218053654	24.704140738636855	22.443991152194307
20	27.21259693425254	26.24734264590703	27.24960623821942	19.29045418162101
21	24.827648532107723	28.34611445342422	25.678001841858382	21.148235172609674
22	24.25959875029048	24.78461445772763	26.65057192284851	24.30521486913338
23	24.449379018306693	27.973439369292606	26.52663378863384	21.05054782376686
24	24.470896055496745	24.48810968524878	28.09522579978827	22.945768459466205
25	23.019356726656167	29.072529628960208	25.53039496673466	22.37771867764896
26	22.602356545913054	27.6506838114419	26.576122974170946	23.1708366684741
27	23.667019546076585	29.248108652430993	25.916410613924107	21.168461187568315
28	22.1655606909551	27.7896838716896	28.758811226729325	21.285944210625974
29	24.118877327067572	26.22926833466739	27.398504135574548	22.25335020269049
30	22.922530059300954	26.972036458467812	28.521693476895006	21.583740005336224
31	26.468537788220715	26.752132338385533	23.8572301548366	22.922099718557153
32	26.414745195245597	28.715777152349233	24.230765920455816	20.638711731949357
33	24.617211908389063	27.617117233425425	25.32899549863582	22.43667535954969
34	24.270357268885505	27.00689405871569	25.626791293346074	23.095957379052734
35	23.778047457977227	26.807215953592056	26.04508249632059	23.36965409211013
36	26.471980514171122	27.72728446383847	24.995911762933893	20.80482325905652
37	23.87917753277045	29.335037482678782	27.081343007393254	19.704441977157515
38	23.96180295558023	27.658860285574114	25.991720244089272	22.387616514756385
39	24.71532959797568	26.962999302847994	25.85745393202338	22.464217167152952
40	24.087032112026304	26.73190632342689	27.96138982846618	21.219671736080628
41	21.915963059550553	27.65756926334271	26.593336603922985	23.833131073183747
42	22.841625999466377	26.941912606401747	27.349875631525038	22.86658576260683
43	26.122113489460958	25.87208551731261	25.708556034668252	22.297244958558185
44	24.00268532624132	26.454336543675282	26.476283921609127	23.06669420847427
45	22.47712738946698	27.29350099408712	25.309199824420975	24.920171792024924
46	23.355883188308503	29.50502207648016	25.559227796569324	21.579866938642017
47	22.74307796913596	25.72275727921368	29.90566930895883	21.628495442691523
48	24.173960942274093	25.690051382684807	27.71910798970625	22.416879685334848
49	21.92026646698856	25.415493988139808	28.61464707755601	24.04959246731562
50	20.171361684181534	26.414314854501797	29.43874960193481	23.975573859381857
51	22.537375093599113	25.034642429875976	26.29252842400613	26.135454052518785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	303.5
2	512.0
3	800.0
4	1088.0
5	880.5
6	673.0
7	618.5
8	564.0
9	512.0
10	460.0
11	473.0
12	486.0
13	470.0
14	454.0
15	509.5
16	565.0
17	582.5
18	600.0
19	567.5
20	535.0
21	646.0
22	757.0
23	895.0
24	1033.0
25	1315.0
26	1981.0
27	2365.0
28	3050.0
29	3735.0
30	4178.5
31	4622.0
32	5169.5
33	5717.0
34	6390.0
35	7063.0
36	7244.5
37	7426.0
38	7910.5
39	8395.0
40	9369.0
41	10343.0
42	12063.5
43	13784.0
44	15387.0
45	16990.0
46	19070.5
47	21151.0
48	19076.5
49	17002.0
50	16339.0
51	15676.0
52	14374.0
53	13072.0
54	12459.0
55	11846.0
56	11788.0
57	11730.0
58	11701.5
59	11673.0
60	11361.5
61	11050.0
62	10198.5
63	9347.0
64	7916.5
65	6486.0
66	5679.5
67	4873.0
68	3981.5
69	3090.0
70	2552.0
71	2014.0
72	1680.0
73	1346.0
74	1131.0
75	699.5
76	483.0
77	385.0
78	287.0
79	248.0
80	209.0
81	169.5
82	130.0
83	102.0
84	74.0
85	52.0
86	30.0
87	19.5
88	9.0
89	10.5
90	12.0
91	8.0
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	232374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.03211202630243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.31015769351447	26.037336362932166
2	9.217575215274309	6.826925559658137
3	4.842364589264755	5.379689638255571
4	3.0213937921978316	4.475543735529793
5	2.0743030457973575	3.8407911384234037
6	1.5560178029818832	3.4573575356967643
7	1.1214019267195796	2.9069517243753604
8	0.9563873426841597	2.8333634571853996
9	0.7588346716558401	2.5291125513181334
>10	6.0741635968531025	37.10268790828578
>50	0.036024310599281836	0.920929191734015
>100	0.027889788851056904	2.1413755411534856
>500	0.0	0.0
>1k	0.003486223606382113	1.547935655451987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	1300	0.5594429669412241	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	1257	0.5409383149577836	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	1040	0.4475543735529793	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	370	0.15922607520634838	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	352	0.15147994181793142	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	336	0.14459448991711638	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	281	0.12092574900806458	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	273	0.11748302305765707	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	260	0.11188859338824482	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	249	0.10715484520643445	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06282974859493747	0.0
2	0.0	0.0	0.0	0.3528794099167721	0.0
3	0.0	0.0	0.0	0.4501364180157849	0.0
4	0.0	0.0	0.0	0.6665978121476586	0.0
5	0.0	0.0	0.0	1.716198886278155	0.0
6	0.0	0.0	0.0	2.0226014958644254	0.0
7	0.0	0.0	0.0	2.4550939433843717	0.0
8	0.0	0.0	0.0	3.0618743921436993	0.0
9	0.0	0.0	0.0	3.2804874899945777	0.0
10	0.0	0.0	0.0	4.612392092058492	0.0
11	0.0	0.0	0.0	5.2557515040409	0.0
12	0.0	0.0	0.0	6.535154535361099	0.0
13	0.0	0.0	0.0	6.793789322385464	0.0
14	0.0	0.0	0.0	6.91041166395552	0.0
15	0.0	0.0	0.0	7.144517028583232	0.0
16	0.0	0.0	0.0	7.478461445772763	0.0
17	0.0	0.0	0.0	7.8231643815573175	0.0
18	0.0	0.0	0.0	8.240594903044231	0.0
19	0.0	0.0	0.0	8.44629777858108	0.0
20	0.0	0.0	0.0	8.671365987588972	0.0
21	0.0	0.0	0.0	8.939037930233159	0.0
22	0.0	0.0	0.0	9.24630122130703	0.0
23	0.0	0.0	0.0	9.53807224560407	0.0
24	0.0	0.0	0.0	9.754533639735943	0.0
25	0.0	0.0	0.0	9.957654470809988	0.0
26	0.0	0.0	0.0	10.15130780552041	0.0
27	0.0	0.0	0.0	10.332911599404408	0.0
28	0.0	0.0	0.0	10.545499926842073	0.0
29	0.0	0.0	0.0	10.779174950725984	0.0
30	0.0	0.0	0.0	11.036949056262749	0.0
31	0.0	0.0	0.0	11.27492748758467	0.0
32	0.0	0.0	0.0	11.505590126261975	0.0
33	0.0	0.0	0.0	11.71817845369964	0.0
34	0.0	0.0	0.0	11.936791551550519	0.0
35	0.0	0.0	0.0	12.181655434773253	0.0
36	0.0	0.0	0.0	12.40414159931834	0.0
37	0.0	0.0	0.0	12.646423438078271	0.0
38	0.0	0.0	0.0	12.871491647086163	0.0
39	0.0	0.0	0.0	13.101723945019666	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATAC	70	0.0	45.000004	3
AGTACGG	35	1.2063356E-7	45.000004	2
GCGACGC	25	3.8811704E-5	45.0	33
GTATTGC	20	7.021659E-4	45.0	9
GTATTAC	20	7.021659E-4	45.0	30
CTATCGA	20	7.021659E-4	45.0	35
ACCGAAA	25	3.8811704E-5	45.0	19
CGGGCAT	25	3.8811704E-5	45.0	6
TACTCGT	20	7.021659E-4	45.0	16
TTACGGG	75	0.0	45.0	3
GATCTAT	20	7.021659E-4	45.0	9
CGACTAA	20	7.021659E-4	45.0	36
AGTAACG	20	7.021659E-4	45.0	1
GCATATC	25	3.8811704E-5	45.0	28
CCTTATC	65	0.0	41.538464	43
TACGGGG	150	0.0	40.5	4
GTACGGG	100	0.0	40.5	3
CCGTCAG	40	3.4423647E-7	39.375	30
TGCATAG	40	3.4423647E-7	39.375	1
ACGGGCT	35	6.2267954E-6	38.57143	5
>>END_MODULE