##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936265.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231810
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558440101807516	31.0	31.0	34.0	30.0	34.0
2	31.674944998058756	31.0	31.0	34.0	30.0	34.0
3	31.571075449721754	31.0	31.0	34.0	30.0	34.0
4	35.38706268064363	37.0	35.0	37.0	33.0	37.0
5	30.934032181527975	35.0	35.0	37.0	0.0	37.0
6	33.16512229843406	36.0	35.0	37.0	17.0	37.0
7	29.654825072257452	35.0	32.0	37.0	0.0	37.0
8	32.58483240584962	35.0	33.0	37.0	17.0	37.0
9	36.33045166299987	39.0	35.0	39.0	32.0	39.0
10	36.529291229886546	38.0	35.0	39.0	32.0	39.0
11	36.67911220396014	38.0	35.0	39.0	32.0	39.0
12	36.718161425305205	39.0	35.0	39.0	33.0	39.0
13	36.43549458608343	39.0	35.0	39.0	32.0	39.0
14	37.59447392260903	39.0	36.0	41.0	33.0	41.0
15	37.70387817609249	39.0	36.0	41.0	33.0	41.0
16	37.78146758120875	39.0	36.0	41.0	33.0	41.0
17	37.73354471334282	39.0	36.0	41.0	33.0	41.0
18	37.64634830248911	39.0	36.0	41.0	33.0	41.0
19	37.532216038997454	39.0	36.0	41.0	32.0	41.0
20	37.46839653164229	39.0	36.0	41.0	32.0	41.0
21	37.371696648116995	39.0	36.0	40.0	32.0	41.0
22	37.37847375005392	39.0	35.0	40.0	32.0	41.0
23	37.31329537120918	39.0	35.0	40.0	32.0	41.0
24	37.27346533799232	39.0	35.0	40.0	32.0	41.0
25	37.16856908675208	39.0	35.0	40.0	32.0	41.0
26	36.99721323497692	39.0	35.0	40.0	31.0	41.0
27	37.010228204132694	39.0	35.0	40.0	31.0	41.0
28	36.90209223070618	39.0	35.0	40.0	31.0	41.0
29	36.892075406582975	39.0	35.0	40.0	31.0	41.0
30	36.79752814805229	39.0	35.0	40.0	31.0	41.0
31	36.631400716103705	39.0	35.0	40.0	30.0	41.0
32	36.50621629783012	39.0	35.0	40.0	30.0	41.0
33	36.345399249385274	38.0	35.0	40.0	30.0	41.0
34	36.21065527802942	38.0	35.0	40.0	30.0	41.0
35	36.09400370993486	38.0	35.0	40.0	30.0	41.0
36	36.002506362969676	38.0	35.0	40.0	29.0	41.0
37	35.895703377766274	38.0	35.0	40.0	29.0	41.0
38	35.748915059747205	38.0	35.0	40.0	28.0	41.0
39	35.75711574133989	38.0	35.0	40.0	28.0	41.0
40	35.62552952849316	38.0	35.0	40.0	27.0	41.0
41	35.58328803761702	38.0	35.0	40.0	27.0	41.0
42	35.530374013200465	38.0	35.0	40.0	27.0	41.0
43	35.338462533971786	38.0	34.0	40.0	26.0	41.0
44	35.10806263750485	38.0	34.0	40.0	26.0	41.0
45	34.99016867261982	38.0	34.0	40.0	25.0	41.0
46	34.80897286570899	38.0	34.0	40.0	24.0	41.0
47	34.62177645485527	38.0	33.0	40.0	24.0	41.0
48	34.53760407230059	37.0	33.0	40.0	23.0	41.0
49	34.36492817393555	37.0	33.0	40.0	23.0	41.0
50	34.11204866054096	37.0	33.0	40.0	22.0	41.0
51	31.829213580087142	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	7.0
11	5.0
12	2.0
13	4.0
14	9.0
15	7.0
16	20.0
17	46.0
18	87.0
19	197.0
20	305.0
21	502.0
22	814.0
23	1184.0
24	1689.0
25	2271.0
26	2717.0
27	3352.0
28	3854.0
29	4640.0
30	5886.0
31	7267.0
32	9588.0
33	13368.0
34	18166.0
35	22283.0
36	24886.0
37	35383.0
38	40446.0
39	32812.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52413614598162	21.85712436909538	27.638151934774168	9.980587550148828
2	30.08110090160045	27.004874681851515	26.91730296363401	15.996721452914025
3	29.896898321901556	25.988525085199086	27.764117164919544	16.35045942797981
4	26.314222854924292	26.233553341098315	29.506492386005778	17.945731417971615
5	21.48915059747207	38.867175704240545	22.77339200207066	16.87028169621673
6	23.25611492170312	35.71502523618481	26.683922177645485	14.34493766446659
7	68.45649454294465	18.45218066519995	8.851214356585134	4.240110435270265
8	80.09835641257926	5.788792545619257	8.946982442517578	5.165868599283896
9	74.98511712178076	6.709805444113714	10.9977999223502	7.307277511755316
10	39.25887580345973	26.6399206246495	18.623872999439193	15.477330572451578
11	28.864587377593722	25.611492170311895	26.340968896941458	19.182951555152925
12	27.449635477330574	23.225486389715716	30.188947845218067	19.135930287735643
13	22.55554117596307	26.90522410594884	32.766058409904666	17.773176308183423
14	19.41374401449463	30.872697467753763	29.81234631810534	19.901212199646263
15	17.424614986411285	27.81976618782624	36.50705319011259	18.248565635649886
16	20.46934989862387	26.627841766964323	32.15089944350977	20.751908890902033
17	20.731202277727448	26.484621025840127	28.48841723825547	24.295759458176956
18	21.30883050774341	26.774944998058753	32.00034511021958	19.915879383978258
19	21.72943358785212	30.08455200379621	26.993227211940813	21.192787196410855
20	23.11160001725551	28.60100944739226	28.896510072904537	19.390880462447694
21	21.369224796169277	29.80932660368405	29.38699797247746	19.43445062766921
22	20.08153228937492	26.250808852077135	28.177818040636726	25.48984081791122
23	19.044044691773436	28.940080238126054	28.935334972606874	23.08054009749364
24	20.42017169233424	26.20335619688538	30.609119537552303	22.767352573228074
25	18.83740994780208	29.866701177688626	28.35468702816962	22.941201846339677
26	17.897847375005394	30.385660670376602	28.98623872999439	22.730253224623613
27	19.7355592942496	29.65143867822786	28.496182218195937	22.116819809326604
28	17.122643544281953	28.294292739743753	31.656960441741084	22.92610327423321
29	18.257624778913765	28.42931711315301	29.628143738406454	23.68491436952677
30	20.72386868556145	28.855528234329842	27.75764634830249	22.662956731806222
31	20.06211983952375	29.877485872050386	26.999698028557873	23.060696259867996
32	21.287261119019888	32.993831154825074	24.587808981493463	21.131098744661575
33	21.30451662999871	29.075535999309782	24.98554850955524	24.634398861136276
34	18.843880764419136	29.570337776627408	27.451792416202924	24.133989042750528
35	18.173504162892023	30.658729131616408	25.244812562011994	25.922954143479576
36	20.535783615892324	28.884431215219358	26.639057849100556	23.940727319787758
37	17.77403908373237	30.122082740175145	28.725680514214226	23.37819766187826
38	19.179069065182695	30.856736120098354	26.12743194857858	23.836762866140372
39	20.53966610586256	27.96686941892067	26.765023079245935	24.728441395970837
40	21.558172641387344	28.43363099089772	26.691255769811484	23.316940597903454
41	17.645485526940167	29.482766058409904	25.3366981579742	27.535050256675724
42	19.541003407963416	27.75246969500884	28.195073551615547	24.51145334541219
43	20.50342953280704	27.370691514602473	27.20978387472499	24.916095077865492
44	19.57853414434235	28.46080842068936	27.172684526120527	24.787972908847763
45	20.235106337086407	27.97592856218455	25.943660756654157	25.845304344074886
46	22.409300720417583	29.21099176049351	24.887623484750442	23.49208403433847
47	17.791294594711186	28.02424399292524	29.019024200854147	25.165437211509428
48	18.82274276347008	26.936715413485178	28.323627108407745	25.916914714636984
49	19.7355592942496	24.51964971312713	30.199732539579827	25.54505845304344
50	19.342565031707	25.43203485613218	28.765368189465512	26.46003192269531
51	18.899098399551356	25.45144730598335	26.042448557007898	29.6070057374574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	233.5
2	363.0
3	882.0
4	1401.0
5	1123.0
6	845.0
7	750.0
8	655.0
9	665.5
10	676.0
11	698.5
12	721.0
13	723.5
14	726.0
15	760.0
16	794.0
17	872.0
18	950.0
19	895.0
20	840.0
21	1010.0
22	1180.0
23	1264.0
24	1348.0
25	1459.5
26	1887.5
27	2204.0
28	2560.0
29	2916.0
30	3317.5
31	3719.0
32	4503.5
33	5288.0
34	6120.5
35	6953.0
36	7310.0
37	7667.0
38	8537.5
39	9408.0
40	10947.0
41	12486.0
42	14632.5
43	16779.0
44	18928.0
45	21077.0
46	24285.0
47	27493.0
48	27102.5
49	26712.0
50	24607.5
51	22503.0
52	18686.5
53	14870.0
54	12303.0
55	9736.0
56	8613.5
57	7491.0
58	6603.5
59	5716.0
60	5238.0
61	4760.0
62	4246.0
63	3732.0
64	3147.0
65	2562.0
66	2189.0
67	1816.0
68	1503.0
69	1190.0
70	1060.0
71	930.0
72	790.0
73	650.0
74	543.5
75	356.0
76	275.0
77	200.5
78	126.0
79	92.5
80	59.0
81	48.0
82	37.0
83	30.0
84	23.0
85	15.5
86	8.0
87	7.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	231810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.40646469593009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1541032865647	35.627903442088126
2	10.864520333796932	10.300969992847177
3	4.120897303543261	5.860715176079314
4	2.3174488907688424	4.3944823609942185
5	1.4710348296746734	3.4868280359727963
6	0.9681194865373093	2.753707335598375
7	0.759124824972964	2.519119695041945
8	0.5830161346667432	2.2110987044189265
9	0.4357685526314788	1.8592421855328674
>10	3.262292458454958	26.829289310784432
>50	0.03979618649257454	1.2192129945829018
>100	0.01989809324628727	1.5814395245015358
>500	0.003979618649257454	1.3559912415573858
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	833	0.35934601613390277	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	774	0.33389413744014496	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.28255899227815884	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	613	0.264440705750399	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	437	0.18851645744359605	TruSeq Adapter, Index 16 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	277	0.11949441352832062	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	246	0.106121392519736	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1760062119839524	0.0
2	0.0	0.0	0.0	0.7972046072214314	0.0
3	0.0	0.0	0.0	1.119882662525344	0.0
4	0.0	0.0	0.0	1.6315085630473232	0.0
5	0.0	0.0	0.0	3.158189896898322	0.0
6	0.0	0.0	0.0	4.0628100599629	0.0
7	0.0	0.0	0.0	5.0929640653983865	0.0
8	0.0	0.0	0.0	6.985462232000345	0.0
9	0.0	0.0	0.0	7.716233121953324	0.0
10	0.0	0.0	0.0	9.877485872050386	0.0
11	0.0	0.0	0.0	11.909753677580778	0.0
12	0.0	0.0	0.0	13.794918252016737	0.0
13	0.0	0.0	0.0	14.461412363573617	0.0
14	0.0	0.0	0.0	14.716362538285665	0.0
15	0.0	0.0	0.0	15.193477416850007	0.0
16	0.0	0.0	0.0	16.209395625727968	0.0
17	0.0	0.0	0.0	17.50269617359044	0.0
18	0.0	0.0	0.0	18.95474742245805	0.0
19	0.0	0.0	0.0	19.803718562615934	0.0
20	0.0	0.0	0.0	20.568137698977612	0.0
21	0.0	0.0	0.0	21.598723092187566	0.0
22	0.0	0.0	0.0	22.68883999827445	0.0
23	0.0	0.0	0.0	23.8509986626979	0.0
24	0.0	0.0	0.0	24.64000690220439	0.0
25	0.0	0.0	0.0	25.381993874293602	0.0
26	0.0	0.0	0.0	26.101548682110348	0.0
27	0.0	0.0	0.0	26.755963935982056	0.0
28	0.0	0.0	0.0	27.42979163970493	0.0
29	0.0	0.0	0.0	28.20758379707519	0.0
30	0.0	0.0	0.0	29.005651179845565	0.0
31	4.313877744704715E-4	0.0	0.0	29.797247745998877	0.0
32	4.313877744704715E-4	0.0	0.0	30.497390103964452	0.0
33	4.313877744704715E-4	0.0	0.0	31.19882662525344	0.0
34	4.313877744704715E-4	0.0	0.0	31.851947715801735	0.0
35	4.313877744704715E-4	0.0	0.0	32.57926750355895	0.0
36	4.313877744704715E-4	0.0	0.0	33.21858418532419	0.0
37	4.313877744704715E-4	0.0	0.0	33.91398127777059	0.0
38	4.313877744704715E-4	0.0	0.0	34.53086579526336	0.0
39	4.313877744704715E-4	0.0	0.0	35.20987015227988	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAC	20	7.021623E-4	45.000004	22
AGCGTTG	20	7.021623E-4	45.000004	1
GCCCATA	20	7.021623E-4	45.000004	13
CGAACTA	25	3.8811413E-5	45.000004	34
TTGGACG	30	2.1579817E-6	45.000004	1
CAAGTAA	25	3.8811413E-5	45.000004	19
GTACAAC	20	7.021623E-4	45.000004	16
CGCGGGT	20	7.021623E-4	45.000004	4
CCGATAT	20	7.021623E-4	45.000004	12
TGTGGTA	25	3.8811413E-5	45.000004	39
GGCCGAT	20	7.021623E-4	45.000004	8
TAAACGG	25	3.8811413E-5	45.000004	2
CACGCCT	20	7.021623E-4	45.000004	33
CCTGTTA	20	7.021623E-4	45.000004	14
TAACGCG	20	7.021623E-4	45.000004	1
TATGCCA	20	7.021623E-4	45.000004	10
TAGCCGT	20	7.021623E-4	45.000004	44
TCGGCCG	20	7.021623E-4	45.000004	16
CGTTATT	45	3.8198777E-10	45.000004	1
ACCTATT	20	7.021623E-4	45.000004	33
>>END_MODULE