##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936265.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 231810 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.558440101807516 31.0 31.0 34.0 30.0 34.0 2 31.674944998058756 31.0 31.0 34.0 30.0 34.0 3 31.571075449721754 31.0 31.0 34.0 30.0 34.0 4 35.38706268064363 37.0 35.0 37.0 33.0 37.0 5 30.934032181527975 35.0 35.0 37.0 0.0 37.0 6 33.16512229843406 36.0 35.0 37.0 17.0 37.0 7 29.654825072257452 35.0 32.0 37.0 0.0 37.0 8 32.58483240584962 35.0 33.0 37.0 17.0 37.0 9 36.33045166299987 39.0 35.0 39.0 32.0 39.0 10 36.529291229886546 38.0 35.0 39.0 32.0 39.0 11 36.67911220396014 38.0 35.0 39.0 32.0 39.0 12 36.718161425305205 39.0 35.0 39.0 33.0 39.0 13 36.43549458608343 39.0 35.0 39.0 32.0 39.0 14 37.59447392260903 39.0 36.0 41.0 33.0 41.0 15 37.70387817609249 39.0 36.0 41.0 33.0 41.0 16 37.78146758120875 39.0 36.0 41.0 33.0 41.0 17 37.73354471334282 39.0 36.0 41.0 33.0 41.0 18 37.64634830248911 39.0 36.0 41.0 33.0 41.0 19 37.532216038997454 39.0 36.0 41.0 32.0 41.0 20 37.46839653164229 39.0 36.0 41.0 32.0 41.0 21 37.371696648116995 39.0 36.0 40.0 32.0 41.0 22 37.37847375005392 39.0 35.0 40.0 32.0 41.0 23 37.31329537120918 39.0 35.0 40.0 32.0 41.0 24 37.27346533799232 39.0 35.0 40.0 32.0 41.0 25 37.16856908675208 39.0 35.0 40.0 32.0 41.0 26 36.99721323497692 39.0 35.0 40.0 31.0 41.0 27 37.010228204132694 39.0 35.0 40.0 31.0 41.0 28 36.90209223070618 39.0 35.0 40.0 31.0 41.0 29 36.892075406582975 39.0 35.0 40.0 31.0 41.0 30 36.79752814805229 39.0 35.0 40.0 31.0 41.0 31 36.631400716103705 39.0 35.0 40.0 30.0 41.0 32 36.50621629783012 39.0 35.0 40.0 30.0 41.0 33 36.345399249385274 38.0 35.0 40.0 30.0 41.0 34 36.21065527802942 38.0 35.0 40.0 30.0 41.0 35 36.09400370993486 38.0 35.0 40.0 30.0 41.0 36 36.002506362969676 38.0 35.0 40.0 29.0 41.0 37 35.895703377766274 38.0 35.0 40.0 29.0 41.0 38 35.748915059747205 38.0 35.0 40.0 28.0 41.0 39 35.75711574133989 38.0 35.0 40.0 28.0 41.0 40 35.62552952849316 38.0 35.0 40.0 27.0 41.0 41 35.58328803761702 38.0 35.0 40.0 27.0 41.0 42 35.530374013200465 38.0 35.0 40.0 27.0 41.0 43 35.338462533971786 38.0 34.0 40.0 26.0 41.0 44 35.10806263750485 38.0 34.0 40.0 26.0 41.0 45 34.99016867261982 38.0 34.0 40.0 25.0 41.0 46 34.80897286570899 38.0 34.0 40.0 24.0 41.0 47 34.62177645485527 38.0 33.0 40.0 24.0 41.0 48 34.53760407230059 37.0 33.0 40.0 23.0 41.0 49 34.36492817393555 37.0 33.0 40.0 23.0 41.0 50 34.11204866054096 37.0 33.0 40.0 22.0 41.0 51 31.829213580087142 35.0 29.0 39.0 14.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 6.0 10 7.0 11 5.0 12 2.0 13 4.0 14 9.0 15 7.0 16 20.0 17 46.0 18 87.0 19 197.0 20 305.0 21 502.0 22 814.0 23 1184.0 24 1689.0 25 2271.0 26 2717.0 27 3352.0 28 3854.0 29 4640.0 30 5886.0 31 7267.0 32 9588.0 33 13368.0 34 18166.0 35 22283.0 36 24886.0 37 35383.0 38 40446.0 39 32812.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.52413614598162 21.85712436909538 27.638151934774168 9.980587550148828 2 30.08110090160045 27.004874681851515 26.91730296363401 15.996721452914025 3 29.896898321901556 25.988525085199086 27.764117164919544 16.35045942797981 4 26.314222854924292 26.233553341098315 29.506492386005778 17.945731417971615 5 21.48915059747207 38.867175704240545 22.77339200207066 16.87028169621673 6 23.25611492170312 35.71502523618481 26.683922177645485 14.34493766446659 7 68.45649454294465 18.45218066519995 8.851214356585134 4.240110435270265 8 80.09835641257926 5.788792545619257 8.946982442517578 5.165868599283896 9 74.98511712178076 6.709805444113714 10.9977999223502 7.307277511755316 10 39.25887580345973 26.6399206246495 18.623872999439193 15.477330572451578 11 28.864587377593722 25.611492170311895 26.340968896941458 19.182951555152925 12 27.449635477330574 23.225486389715716 30.188947845218067 19.135930287735643 13 22.55554117596307 26.90522410594884 32.766058409904666 17.773176308183423 14 19.41374401449463 30.872697467753763 29.81234631810534 19.901212199646263 15 17.424614986411285 27.81976618782624 36.50705319011259 18.248565635649886 16 20.46934989862387 26.627841766964323 32.15089944350977 20.751908890902033 17 20.731202277727448 26.484621025840127 28.48841723825547 24.295759458176956 18 21.30883050774341 26.774944998058753 32.00034511021958 19.915879383978258 19 21.72943358785212 30.08455200379621 26.993227211940813 21.192787196410855 20 23.11160001725551 28.60100944739226 28.896510072904537 19.390880462447694 21 21.369224796169277 29.80932660368405 29.38699797247746 19.43445062766921 22 20.08153228937492 26.250808852077135 28.177818040636726 25.48984081791122 23 19.044044691773436 28.940080238126054 28.935334972606874 23.08054009749364 24 20.42017169233424 26.20335619688538 30.609119537552303 22.767352573228074 25 18.83740994780208 29.866701177688626 28.35468702816962 22.941201846339677 26 17.897847375005394 30.385660670376602 28.98623872999439 22.730253224623613 27 19.7355592942496 29.65143867822786 28.496182218195937 22.116819809326604 28 17.122643544281953 28.294292739743753 31.656960441741084 22.92610327423321 29 18.257624778913765 28.42931711315301 29.628143738406454 23.68491436952677 30 20.72386868556145 28.855528234329842 27.75764634830249 22.662956731806222 31 20.06211983952375 29.877485872050386 26.999698028557873 23.060696259867996 32 21.287261119019888 32.993831154825074 24.587808981493463 21.131098744661575 33 21.30451662999871 29.075535999309782 24.98554850955524 24.634398861136276 34 18.843880764419136 29.570337776627408 27.451792416202924 24.133989042750528 35 18.173504162892023 30.658729131616408 25.244812562011994 25.922954143479576 36 20.535783615892324 28.884431215219358 26.639057849100556 23.940727319787758 37 17.77403908373237 30.122082740175145 28.725680514214226 23.37819766187826 38 19.179069065182695 30.856736120098354 26.12743194857858 23.836762866140372 39 20.53966610586256 27.96686941892067 26.765023079245935 24.728441395970837 40 21.558172641387344 28.43363099089772 26.691255769811484 23.316940597903454 41 17.645485526940167 29.482766058409904 25.3366981579742 27.535050256675724 42 19.541003407963416 27.75246969500884 28.195073551615547 24.51145334541219 43 20.50342953280704 27.370691514602473 27.20978387472499 24.916095077865492 44 19.57853414434235 28.46080842068936 27.172684526120527 24.787972908847763 45 20.235106337086407 27.97592856218455 25.943660756654157 25.845304344074886 46 22.409300720417583 29.21099176049351 24.887623484750442 23.49208403433847 47 17.791294594711186 28.02424399292524 29.019024200854147 25.165437211509428 48 18.82274276347008 26.936715413485178 28.323627108407745 25.916914714636984 49 19.7355592942496 24.51964971312713 30.199732539579827 25.54505845304344 50 19.342565031707 25.43203485613218 28.765368189465512 26.46003192269531 51 18.899098399551356 25.45144730598335 26.042448557007898 29.6070057374574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 233.5 2 363.0 3 882.0 4 1401.0 5 1123.0 6 845.0 7 750.0 8 655.0 9 665.5 10 676.0 11 698.5 12 721.0 13 723.5 14 726.0 15 760.0 16 794.0 17 872.0 18 950.0 19 895.0 20 840.0 21 1010.0 22 1180.0 23 1264.0 24 1348.0 25 1459.5 26 1887.5 27 2204.0 28 2560.0 29 2916.0 30 3317.5 31 3719.0 32 4503.5 33 5288.0 34 6120.5 35 6953.0 36 7310.0 37 7667.0 38 8537.5 39 9408.0 40 10947.0 41 12486.0 42 14632.5 43 16779.0 44 18928.0 45 21077.0 46 24285.0 47 27493.0 48 27102.5 49 26712.0 50 24607.5 51 22503.0 52 18686.5 53 14870.0 54 12303.0 55 9736.0 56 8613.5 57 7491.0 58 6603.5 59 5716.0 60 5238.0 61 4760.0 62 4246.0 63 3732.0 64 3147.0 65 2562.0 66 2189.0 67 1816.0 68 1503.0 69 1190.0 70 1060.0 71 930.0 72 790.0 73 650.0 74 543.5 75 356.0 76 275.0 77 200.5 78 126.0 79 92.5 80 59.0 81 48.0 82 37.0 83 30.0 84 23.0 85 15.5 86 8.0 87 7.5 88 7.0 89 5.5 90 4.0 91 2.5 92 1.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 231810.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.40646469593009 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1541032865647 35.627903442088126 2 10.864520333796932 10.300969992847177 3 4.120897303543261 5.860715176079314 4 2.3174488907688424 4.3944823609942185 5 1.4710348296746734 3.4868280359727963 6 0.9681194865373093 2.753707335598375 7 0.759124824972964 2.519119695041945 8 0.5830161346667432 2.2110987044189265 9 0.4357685526314788 1.8592421855328674 >10 3.262292458454958 26.829289310784432 >50 0.03979618649257454 1.2192129945829018 >100 0.01989809324628727 1.5814395245015358 >500 0.003979618649257454 1.3559912415573858 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG 833 0.35934601613390277 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC 774 0.33389413744014496 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.28255899227815884 No Hit GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC 613 0.264440705750399 No Hit GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC 437 0.18851645744359605 TruSeq Adapter, Index 16 (95% over 21bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 277 0.11949441352832062 No Hit CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT 246 0.106121392519736 TruSeq Adapter, Index 13 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1760062119839524 0.0 2 0.0 0.0 0.0 0.7972046072214314 0.0 3 0.0 0.0 0.0 1.119882662525344 0.0 4 0.0 0.0 0.0 1.6315085630473232 0.0 5 0.0 0.0 0.0 3.158189896898322 0.0 6 0.0 0.0 0.0 4.0628100599629 0.0 7 0.0 0.0 0.0 5.0929640653983865 0.0 8 0.0 0.0 0.0 6.985462232000345 0.0 9 0.0 0.0 0.0 7.716233121953324 0.0 10 0.0 0.0 0.0 9.877485872050386 0.0 11 0.0 0.0 0.0 11.909753677580778 0.0 12 0.0 0.0 0.0 13.794918252016737 0.0 13 0.0 0.0 0.0 14.461412363573617 0.0 14 0.0 0.0 0.0 14.716362538285665 0.0 15 0.0 0.0 0.0 15.193477416850007 0.0 16 0.0 0.0 0.0 16.209395625727968 0.0 17 0.0 0.0 0.0 17.50269617359044 0.0 18 0.0 0.0 0.0 18.95474742245805 0.0 19 0.0 0.0 0.0 19.803718562615934 0.0 20 0.0 0.0 0.0 20.568137698977612 0.0 21 0.0 0.0 0.0 21.598723092187566 0.0 22 0.0 0.0 0.0 22.68883999827445 0.0 23 0.0 0.0 0.0 23.8509986626979 0.0 24 0.0 0.0 0.0 24.64000690220439 0.0 25 0.0 0.0 0.0 25.381993874293602 0.0 26 0.0 0.0 0.0 26.101548682110348 0.0 27 0.0 0.0 0.0 26.755963935982056 0.0 28 0.0 0.0 0.0 27.42979163970493 0.0 29 0.0 0.0 0.0 28.20758379707519 0.0 30 0.0 0.0 0.0 29.005651179845565 0.0 31 4.313877744704715E-4 0.0 0.0 29.797247745998877 0.0 32 4.313877744704715E-4 0.0 0.0 30.497390103964452 0.0 33 4.313877744704715E-4 0.0 0.0 31.19882662525344 0.0 34 4.313877744704715E-4 0.0 0.0 31.851947715801735 0.0 35 4.313877744704715E-4 0.0 0.0 32.57926750355895 0.0 36 4.313877744704715E-4 0.0 0.0 33.21858418532419 0.0 37 4.313877744704715E-4 0.0 0.0 33.91398127777059 0.0 38 4.313877744704715E-4 0.0 0.0 34.53086579526336 0.0 39 4.313877744704715E-4 0.0 0.0 35.20987015227988 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATCAC 20 7.021623E-4 45.000004 22 AGCGTTG 20 7.021623E-4 45.000004 1 GCCCATA 20 7.021623E-4 45.000004 13 CGAACTA 25 3.8811413E-5 45.000004 34 TTGGACG 30 2.1579817E-6 45.000004 1 CAAGTAA 25 3.8811413E-5 45.000004 19 GTACAAC 20 7.021623E-4 45.000004 16 CGCGGGT 20 7.021623E-4 45.000004 4 CCGATAT 20 7.021623E-4 45.000004 12 TGTGGTA 25 3.8811413E-5 45.000004 39 GGCCGAT 20 7.021623E-4 45.000004 8 TAAACGG 25 3.8811413E-5 45.000004 2 CACGCCT 20 7.021623E-4 45.000004 33 CCTGTTA 20 7.021623E-4 45.000004 14 TAACGCG 20 7.021623E-4 45.000004 1 TATGCCA 20 7.021623E-4 45.000004 10 TAGCCGT 20 7.021623E-4 45.000004 44 TCGGCCG 20 7.021623E-4 45.000004 16 CGTTATT 45 3.8198777E-10 45.000004 1 ACCTATT 20 7.021623E-4 45.000004 33 >>END_MODULE