FastQCFastQC Report
Sat 14 Jan 2017
SRR2936264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560809
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103351.8428734203623693No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34400.613399570976928No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19810.3532396947980507No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.2532056368567551No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT6200.11055457383886491No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.0301915E-445.0000041
GGCCGAT351.2104465E-745.0000048
CGATTAT207.0301915E-445.00000410
ATCGTTC351.2104465E-745.00000430
TACGCTT207.0301915E-445.00000418
AATCGCG207.0301915E-445.0000041
GCGCGAG253.888242E-545.01
TATAGCG253.888242E-545.01
TATCGTT453.8380676E-1045.029
CACGCCG302.163506E-644.99999626
CGAATAT302.163506E-644.99999614
GCGATCA302.163506E-644.9999969
TTGCACG302.163506E-644.9999961
TAGTGCG302.163506E-644.9999961
CGTTATT12000.044.251
CGTTTTA5900.043.8559341
CGGTTTT14150.042.614841
CGGTTTA850.042.352941
CGTTTTT51650.041.9506261
TTAACGG556.002665E-1140.9090922
Produced by FastQC (version 0.11.5)