##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936264.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560809
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58251026641869	31.0	31.0	34.0	30.0	34.0
2	31.64105069640466	31.0	31.0	34.0	30.0	34.0
3	30.72584605453907	31.0	31.0	34.0	26.0	34.0
4	34.94659500828268	37.0	35.0	37.0	32.0	37.0
5	30.792093208204577	35.0	35.0	37.0	0.0	37.0
6	33.1778288151581	36.0	35.0	37.0	17.0	37.0
7	29.829990246233567	35.0	32.0	37.0	0.0	37.0
8	32.83248663983638	35.0	35.0	37.0	17.0	37.0
9	36.63581361925361	39.0	35.0	39.0	32.0	39.0
10	36.945635679883885	39.0	37.0	39.0	34.0	39.0
11	37.05071245290286	39.0	37.0	39.0	34.0	39.0
12	37.203263499694195	39.0	37.0	39.0	34.0	39.0
13	37.163164285879866	39.0	37.0	39.0	34.0	39.0
14	38.50790732673691	40.0	38.0	41.0	34.0	41.0
15	38.56138542712403	40.0	38.0	41.0	34.0	41.0
16	38.56866063133794	40.0	38.0	41.0	34.0	41.0
17	38.52070312708962	40.0	38.0	41.0	34.0	41.0
18	38.29024320223106	40.0	38.0	41.0	34.0	41.0
19	38.036668455748746	39.0	37.0	41.0	34.0	41.0
20	37.722199536740675	39.0	35.0	41.0	34.0	41.0
21	37.60540397889478	39.0	35.0	41.0	33.0	41.0
22	37.5215911299569	39.0	35.0	41.0	33.0	41.0
23	37.439247586968115	39.0	35.0	41.0	33.0	41.0
24	37.37353715792721	39.0	35.0	41.0	33.0	41.0
25	37.252984527709074	39.0	35.0	41.0	33.0	41.0
26	37.13250678929903	39.0	35.0	41.0	33.0	41.0
27	37.105249737432885	39.0	35.0	41.0	33.0	41.0
28	36.98622525672734	39.0	35.0	41.0	33.0	41.0
29	36.93387944915292	39.0	35.0	41.0	32.0	41.0
30	36.778157982486015	39.0	35.0	40.0	32.0	41.0
31	36.554589887109515	39.0	35.0	40.0	31.0	41.0
32	36.23596803902933	39.0	35.0	40.0	30.0	41.0
33	35.7187384653242	38.0	35.0	40.0	27.0	41.0
34	35.216280409194574	38.0	34.0	40.0	24.0	41.0
35	34.734169744066165	38.0	34.0	40.0	21.0	41.0
36	34.359824824494616	38.0	34.0	40.0	18.0	41.0
37	34.09375741116851	38.0	33.0	40.0	15.0	41.0
38	33.87607545527978	38.0	33.0	40.0	15.0	41.0
39	33.76458473383986	38.0	33.0	40.0	15.0	41.0
40	33.6278857864264	38.0	33.0	40.0	14.0	41.0
41	33.51247394389177	38.0	33.0	40.0	12.0	41.0
42	33.42268223227516	38.0	33.0	40.0	11.0	41.0
43	33.230294092997795	38.0	33.0	40.0	10.0	41.0
44	33.050198909075995	38.0	32.0	40.0	10.0	41.0
45	32.94626869397602	38.0	32.0	40.0	10.0	41.0
46	32.84109384835122	38.0	31.0	40.0	10.0	41.0
47	32.70814840703341	38.0	31.0	40.0	10.0	41.0
48	32.58831794782181	37.0	31.0	40.0	10.0	41.0
49	32.427439645226805	37.0	31.0	40.0	10.0	41.0
50	32.27396136652586	37.0	30.0	40.0	9.0	41.0
51	30.630339384710304	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	22.0
10	20.0
11	19.0
12	16.0
13	13.0
14	10.0
15	14.0
16	45.0
17	107.0
18	267.0
19	531.0
20	1081.0
21	1728.0
22	3055.0
23	5039.0
24	8669.0
25	13347.0
26	16740.0
27	16903.0
28	15123.0
29	13788.0
30	13757.0
31	15115.0
32	18115.0
33	23225.0
34	31600.0
35	40131.0
36	48164.0
37	77165.0
38	98901.0
39	98071.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.53566009104704	19.55086312808817	23.210932777469694	23.702544003395097
2	41.24398859504751	22.004461411995884	23.669912572729753	13.081637420226851
3	29.336012795800354	21.905140609369678	35.02832515170049	13.73052144312948
4	23.07559258143147	23.135684341727757	37.71248321621087	16.07623986062991
5	19.163743805823373	36.15633843251446	31.08919436029022	13.590723401371946
6	21.439384888616267	30.640200139441415	35.06755419403041	12.852860777911909
7	61.50614558610864	17.590302580736044	17.658418463327084	3.2451333698282308
8	75.28178042791752	3.5327535756380515	18.663038574630576	2.522427421813844
9	69.76385899655676	6.406815867790995	20.057452715630454	3.77187242002179
10	31.520892139748113	31.463475086883413	26.364591153137702	10.651041620230774
11	20.113443257864976	24.602315583380438	39.5052504506882	15.778990708066381
12	19.474188181716055	22.571142759834455	40.62773600281023	17.326933055639266
13	19.36737819828141	22.481450903961957	41.95760053779451	16.193570359962127
14	16.75472397910875	25.515104072866162	40.48330180150461	17.246870146520475
15	16.68910449012052	26.10193488335601	41.96223669734259	15.246723929180881
16	19.83901827538431	25.760463901256937	39.41965981287747	14.980858010481288
17	19.617909127706582	25.780256736250667	37.973712975362375	16.628121160680372
18	19.663379154043533	25.739601183290567	38.67305981180758	15.923959850858314
19	18.895203179692192	27.532903359254217	37.68466625892238	15.887227202131207
20	19.70528290380504	26.916115825530618	37.803066641227225	15.575534629437115
21	20.109342039803213	25.97765014470167	38.83015429495604	15.082853520539077
22	19.34544559734241	23.512996403410074	39.3110666911551	17.830491308092416
23	17.61116529870241	25.531508945113217	39.593159168272976	17.264166587911394
24	17.73562835118552	24.865328480819674	40.19621653718111	17.2028266308137
25	17.867580584477068	27.042540330130223	37.94464782127248	17.145231264120227
26	17.41412851790895	27.650947113901523	37.7929027529872	17.142021615202324
27	17.624717149689108	26.636698055844327	38.255448824822714	17.483135969643854
28	17.051616504014735	26.755455065806714	38.6058354983604	17.58709293181814
29	17.250079795438374	25.28918045181158	39.0813984796963	18.379341273053747
30	17.715657202363015	25.038827836215184	39.13524925598555	18.110265705436255
31	18.08958130129866	25.142249856903153	38.16878830403934	18.599380537758844
32	18.56443102731946	25.84409308695117	37.31341686741832	18.278059018311048
33	18.50451758085195	25.782396502195937	37.70552897688874	18.007556940063374
34	17.80356591994779	25.85140395393084	38.079988017310704	18.265042108810665
35	17.704245117321584	26.40542501992657	38.030951714398306	17.85937814835354
36	18.40894136862996	26.867436150275765	36.769381375833845	17.954241105260436
37	18.776981111216116	27.479944152108825	36.169890283501154	17.573184453173894
38	18.612397447259227	27.704441262533237	35.375145548662736	18.308015741544803
39	19.5945500161374	27.23458432371806	34.831288370907025	18.339577289237514
40	19.91979443981819	26.870289171536122	35.42560836220531	17.784308026440375
41	18.70173267547418	27.980114441815306	34.44666544224504	18.87148744046547
42	19.0842158381909	28.05875084030392	33.835940578699706	19.021092742805482
43	19.631282664864507	27.761145060082843	33.817395940507375	18.790176334545272
44	19.50004368688805	28.12454864312092	32.79565770164174	19.579749968349294
45	18.901622477527997	27.63989165651764	33.26248330536778	20.19600256058658
46	18.94370454111828	28.28092987095428	32.926896679618196	19.848468908309247
47	18.39521120381449	27.688036390286175	34.23910814555401	19.67764426034532
48	18.772166637839263	26.869754230049804	34.32362890039211	20.03445023171882
49	18.74238822843428	26.662731874845093	34.197739337278826	20.397140559441805
50	18.41857031538367	26.481208397154827	34.635856414572515	20.46436487288899
51	18.224029928193023	26.53577242875917	33.97413379599828	21.26606384704953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	375.0
2	589.0
3	11592.5
4	22596.0
5	17933.0
6	13270.0
7	10705.5
8	8141.0
9	7753.0
10	7365.0
11	7153.5
12	6942.0
13	6710.5
14	6479.0
15	6309.0
16	6139.0
17	5834.5
18	5530.0
19	5323.5
20	5117.0
21	5206.5
22	5296.0
23	5596.0
24	5896.0
25	6448.0
26	8089.5
27	9179.0
28	10262.0
29	11345.0
30	13143.5
31	14942.0
32	16574.0
33	18206.0
34	20025.5
35	21845.0
36	23481.0
37	25117.0
38	26755.0
39	28393.0
40	30051.0
41	31709.0
42	32721.0
43	33733.0
44	35763.5
45	37794.0
46	41283.5
47	44773.0
48	43943.5
49	43114.0
50	39997.5
51	36881.0
52	32400.0
53	27919.0
54	24585.5
55	21252.0
56	18614.0
57	15976.0
58	14467.0
59	12958.0
60	11480.5
61	10003.0
62	8291.0
63	6579.0
64	5147.0
65	3715.0
66	2878.5
67	2042.0
68	1615.0
69	1188.0
70	901.5
71	615.0
72	503.5
73	392.0
74	320.0
75	196.5
76	145.0
77	123.5
78	102.0
79	78.5
80	55.0
81	41.0
82	27.0
83	20.5
84	14.0
85	14.5
86	15.0
87	9.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.803017316211104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54402987905785	38.61926662835509
2	8.667638449646304	8.633490955967842
3	3.832462158507688	5.726045377316465
4	2.2248783620182944	4.432226223602421
5	1.5017482705750105	3.739579756201867
6	1.0625888675918889	3.175207906361519
7	0.834032283828922	2.907612699166763
8	0.674184646834398	2.686114371249375
9	0.545341924629481	2.4443705984020134
>10	3.0818447787142955	22.774373248381547
>50	0.0187502272537376	0.6111929642564113
>100	0.010661893791831798	0.9920441720023386
>500	3.6765151006316546E-4	0.11352295803644333
>1k	0.0011029545301894962	1.2525976708504982
>5k	0.0	0.0
>10k+	3.6765151006316546E-4	1.8923544698494226
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10335	1.8428734203623693	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3440	0.613399570976928	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1981	0.3532396947980507	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1420	0.2532056368567551	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	620	0.11055457383886491	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.566276575447256E-4	0.0	0.0	0.08255930272160397	0.0
2	3.566276575447256E-4	0.0	0.0	0.24446825924690938	0.0
3	3.566276575447256E-4	0.0	0.0	0.41119168914906856	0.0
4	3.566276575447256E-4	0.0	0.0	0.6064453316548059	0.0
5	3.566276575447256E-4	0.0	0.0	1.194880966603603	0.0
6	3.566276575447256E-4	0.0	0.0	1.9746473398251454	0.0
7	3.566276575447256E-4	0.0	0.0	2.552027517390056	0.0
8	3.566276575447256E-4	0.0	0.0	3.3692397946537946	0.0
9	3.566276575447256E-4	0.0	0.0	3.8817137385455656	0.0
10	3.566276575447256E-4	0.0	0.0	4.654525872444986	0.0
11	3.566276575447256E-4	0.0	0.0	5.300378560258483	0.0
12	3.566276575447256E-4	0.0	0.0	5.888635881378509	0.0
13	3.566276575447256E-4	0.0	0.0	6.202289906189095	0.0
14	3.566276575447256E-4	0.0	0.0	6.368121766947392	0.0
15	5.349414863170883E-4	0.0	0.0	6.554281404185739	0.0
16	5.349414863170883E-4	0.0	0.0	6.874889668318447	0.0
17	5.349414863170883E-4	0.0	0.0	7.275917469227491	0.0
18	5.349414863170883E-4	0.0	0.0	7.725446631562618	0.0
19	5.349414863170883E-4	0.0	0.0	8.016454800119114	0.0
20	5.349414863170883E-4	0.0	0.0	8.317626856915634	0.0
21	5.349414863170883E-4	0.0	0.0	8.634133902986578	0.0
22	5.349414863170883E-4	0.0	0.0	8.97542657125688	0.0
23	5.349414863170883E-4	0.0	0.0	9.34524945213076	0.0
24	5.349414863170883E-4	0.0	0.0	9.635900993029713	0.0
25	5.349414863170883E-4	0.0	0.0	9.896417496866134	0.0
26	5.349414863170883E-4	0.0	0.0	10.161926787908182	0.0
27	5.349414863170883E-4	0.0	0.0	10.423869802374783	0.0
28	5.349414863170883E-4	0.0	0.0	10.689557407245605	0.0
29	5.349414863170883E-4	0.0	0.0	10.987698128952994	0.0
30	5.349414863170883E-4	0.0	0.0	11.342899275867541	0.0
31	5.349414863170883E-4	0.0	0.0	11.670105151664828	0.0
32	5.349414863170883E-4	0.0	0.0	11.99107004345508	0.0
33	5.349414863170883E-4	0.0	0.0	12.29527343534073	0.0
34	5.349414863170883E-4	0.0	0.0	12.585568348582138	0.0
35	5.349414863170883E-4	0.0	0.0	12.89583441064605	0.0
36	5.349414863170883E-4	0.0	0.0	13.201820940819424	0.0
37	5.349414863170883E-4	0.0	0.0	13.517614731575279	0.0
38	5.349414863170883E-4	0.0	0.0	13.840541075482028	0.0
39	5.349414863170883E-4	0.0	0.0	14.164180674703866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.0301915E-4	45.000004	1
GGCCGAT	35	1.2104465E-7	45.000004	8
CGATTAT	20	7.0301915E-4	45.000004	10
ATCGTTC	35	1.2104465E-7	45.000004	30
TACGCTT	20	7.0301915E-4	45.000004	18
AATCGCG	20	7.0301915E-4	45.000004	1
GCGCGAG	25	3.888242E-5	45.0	1
TATAGCG	25	3.888242E-5	45.0	1
TATCGTT	45	3.8380676E-10	45.0	29
CACGCCG	30	2.163506E-6	44.999996	26
CGAATAT	30	2.163506E-6	44.999996	14
GCGATCA	30	2.163506E-6	44.999996	9
TTGCACG	30	2.163506E-6	44.999996	1
TAGTGCG	30	2.163506E-6	44.999996	1
CGTTATT	1200	0.0	44.25	1
CGTTTTA	590	0.0	43.855934	1
CGGTTTT	1415	0.0	42.61484	1
CGGTTTA	85	0.0	42.35294	1
CGTTTTT	5165	0.0	41.950626	1
TTAACGG	55	6.002665E-11	40.909092	2
>>END_MODULE