##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936261.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 334858 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.799804693332696 31.0 31.0 34.0 30.0 34.0 2 31.953096536442313 33.0 31.0 34.0 30.0 34.0 3 31.88055533987541 33.0 31.0 34.0 30.0 34.0 4 35.62272366197015 37.0 35.0 37.0 33.0 37.0 5 31.142397673043497 37.0 35.0 37.0 0.0 37.0 6 33.35298843091699 37.0 35.0 37.0 17.0 37.0 7 29.529761869210233 35.0 32.0 37.0 0.0 37.0 8 32.56794223222978 35.0 33.0 37.0 17.0 37.0 9 36.385584337241454 39.0 35.0 39.0 32.0 39.0 10 36.69549182041343 38.0 35.0 39.0 33.0 39.0 11 36.85107717301065 39.0 37.0 39.0 33.0 39.0 12 36.87643120367439 39.0 35.0 39.0 33.0 39.0 13 36.66753668719278 39.0 35.0 39.0 33.0 39.0 14 37.88268161429621 40.0 37.0 41.0 33.0 41.0 15 37.99488439876007 40.0 37.0 41.0 33.0 41.0 16 38.06473191621523 40.0 37.0 41.0 33.0 41.0 17 38.03004258521523 40.0 37.0 41.0 33.0 41.0 18 37.93484103709632 40.0 37.0 41.0 33.0 41.0 19 37.84293043618489 40.0 36.0 41.0 33.0 41.0 20 37.79836229088151 40.0 36.0 41.0 33.0 41.0 21 37.71333819111385 39.0 36.0 41.0 33.0 41.0 22 37.70662489771784 39.0 36.0 41.0 33.0 41.0 23 37.68115439977543 39.0 36.0 41.0 33.0 41.0 24 37.618820514964554 39.0 36.0 41.0 33.0 41.0 25 37.51181097659306 39.0 36.0 41.0 33.0 41.0 26 37.335503407414485 39.0 35.0 41.0 32.0 41.0 27 37.29857432105549 39.0 36.0 41.0 32.0 41.0 28 37.198731999832766 39.0 35.0 41.0 32.0 41.0 29 37.192836963727906 39.0 35.0 41.0 32.0 41.0 30 37.074631634901955 39.0 35.0 41.0 31.0 41.0 31 36.957375962348216 39.0 35.0 41.0 31.0 41.0 32 36.870401782247995 39.0 35.0 41.0 31.0 41.0 33 36.688037914578715 39.0 35.0 41.0 30.0 41.0 34 36.57464059392339 39.0 35.0 40.0 30.0 41.0 35 36.48427094469895 39.0 35.0 40.0 30.0 41.0 36 36.36581177693231 39.0 35.0 40.0 30.0 41.0 37 36.27963494973989 39.0 35.0 40.0 30.0 41.0 38 36.191985856691495 39.0 35.0 40.0 30.0 41.0 39 36.18107974126346 39.0 35.0 40.0 30.0 41.0 40 36.05618202342485 39.0 35.0 40.0 29.0 41.0 41 35.9842918490823 39.0 35.0 40.0 29.0 41.0 42 35.958672034115956 38.0 35.0 40.0 29.0 41.0 43 35.80106791535516 38.0 35.0 40.0 28.0 41.0 44 35.56719266076964 38.0 35.0 40.0 27.0 41.0 45 35.470112704489665 38.0 34.0 40.0 27.0 41.0 46 35.35197307515424 38.0 34.0 40.0 26.0 41.0 47 35.20044317292703 38.0 34.0 40.0 26.0 41.0 48 35.12400181569501 38.0 34.0 40.0 26.0 41.0 49 34.95650992360941 38.0 34.0 40.0 25.0 41.0 50 34.77819553362918 38.0 34.0 40.0 24.0 41.0 51 32.61486361383034 35.0 31.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 15.0 10 18.0 11 16.0 12 9.0 13 12.0 14 12.0 15 22.0 16 29.0 17 80.0 18 141.0 19 250.0 20 390.0 21 600.0 22 985.0 23 1431.0 24 2115.0 25 2676.0 26 3604.0 27 4348.0 28 4972.0 29 5901.0 30 7407.0 31 9451.0 32 12373.0 33 16961.0 34 24274.0 35 29523.0 36 33801.0 37 50690.0 38 63035.0 39 59708.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.124046610802196 22.604805619098244 27.64425517682122 10.626892593278345 2 31.966087117524445 25.968022266154613 25.396735332588737 16.66915528373221 3 31.40405783944239 24.951471967221927 26.98218349270437 16.662286700631313 4 28.56942345710719 26.30428420405067 26.87975201428665 18.246540324555482 5 22.44175142896392 39.736246408925574 20.712361657777326 17.10964050433318 6 25.200532763141393 36.721834329775604 24.497548214467027 13.580084692615973 7 68.3788352077597 19.830793948479656 7.482873337354938 4.3074975064057 8 81.84543896218696 6.450495433885409 7.622932705803655 4.081132898123982 9 75.35253749350471 8.76879154746191 9.44280859349336 6.43586236554002 10 39.20228873134284 29.339899300599058 17.691976897670056 13.765835070388043 11 30.046168823799942 27.353385614200647 25.334619450632808 17.265826111366607 12 28.558373997336183 23.992259405479338 28.85491760686619 18.594448990318284 13 23.72528056668797 26.769854684672307 30.698385584337238 18.80647916430248 14 19.183355332708192 30.850091680652696 28.58166745306966 21.384885533569452 15 18.326872883431186 28.84775038971743 34.078624372121915 18.746752354729466 16 20.499435581649536 27.054751566335582 32.680419760017685 19.765393091997204 17 20.8604841455184 26.695494806753906 28.960335425762562 23.48368562196513 18 21.570337277293657 27.972453995424928 30.027653512832302 20.42955521444911 19 23.828010679153554 28.710378727699503 27.39638891709322 20.065221676053728 20 24.611925054799347 28.933458361454704 27.5239056555316 18.93071092821435 21 22.73471142991955 29.27449844411661 28.459526127492847 19.531263998470994 22 21.271404595380726 26.458379372749047 28.133716381272063 24.136499650598164 23 21.398025431675517 28.512384353964965 28.293784230927738 21.795805983431784 24 21.059673055444396 26.56738080021979 30.131279527441485 22.241666616894324 25 20.9040847165067 28.871342479498775 27.952445514217967 22.27212728977656 26 19.797346935118767 30.863530212806623 27.69711340329334 21.642009448781273 27 20.948581189638592 30.351372820718037 28.116992874591617 20.583053115051754 28 18.316719325803774 29.090838504679596 30.453207031040026 22.139235138476607 29 20.393420494657438 28.22300796158372 29.090838504679596 22.292733039079252 30 20.395809567040356 28.751291592257015 27.985295259483124 22.8676035812195 31 21.96304105023622 29.303167312711658 25.518578024117684 23.215213612934438 32 23.066493857097637 30.00555459329029 25.306547850133487 21.621403699478588 33 21.76026853173584 30.495911699884726 24.90578095789857 22.83803881048086 34 20.28591223742601 29.316605844865585 27.56780486056776 22.82967705714064 35 21.30694204707667 27.24289101649057 26.61128000525596 24.8388869311768 36 19.90246611996727 32.26621433562883 25.322972722766067 22.50834682163783 37 20.399691809662603 29.602398628672454 27.782522740982746 22.215386820682202 38 20.12763619205753 30.040793410938367 25.743150828112217 24.08841956889189 39 22.44593230563403 27.93572200753752 27.35905966111008 22.259286025718364 40 21.002932586350035 27.859271691284064 29.26613669077639 21.87165903158951 41 17.624784236900418 29.068739585137582 29.00572780103805 24.30074837692395 42 19.606221144485126 28.646769675504242 27.81716429053509 23.92984488947554 43 21.855532793004794 27.130305980445442 27.10193574589826 23.9122254806515 44 20.539452544063455 28.77398777989476 26.705349730333456 23.98120994570833 45 20.97366644965926 27.62872620633224 26.345495702656052 25.052111641352454 46 20.675928303937788 29.596724581763016 26.636365265276623 23.090981849022572 47 19.89350709853132 27.451636215948255 28.997963315793562 23.65689336972687 48 20.018336130538916 27.587514707726857 28.051293384061303 24.342855777672927 49 20.040733684128796 26.68802895555728 29.425607272336336 23.84563008797759 50 19.091973314061484 27.129708712349714 29.305556385094576 24.472761588494226 51 20.02042656887397 26.732226794641313 26.137646405341965 27.109700231142753 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 154.0 1 501.5 2 849.0 3 1148.0 4 1447.0 5 1187.0 6 927.0 7 832.0 8 737.0 9 783.0 10 829.0 11 810.5 12 792.0 13 743.0 14 694.0 15 720.5 16 747.0 17 822.5 18 898.0 19 1117.0 20 1336.0 21 1501.5 22 1667.0 23 2008.0 24 2349.0 25 2791.5 26 3515.5 27 3797.0 28 5143.0 29 6489.0 30 7528.5 31 8568.0 32 9163.5 33 9759.0 34 10800.0 35 11841.0 36 12214.0 37 12587.0 38 13586.0 39 14585.0 40 16043.5 41 17502.0 42 20022.5 43 22543.0 44 24523.0 45 26503.0 46 29350.5 47 32198.0 48 31685.0 49 31172.0 50 29154.0 51 27136.0 52 23472.0 53 19808.0 54 17751.5 55 15695.0 56 14096.0 57 12497.0 58 12150.5 59 11804.0 60 11244.0 61 10684.0 62 9547.5 63 8411.0 64 6891.5 65 5372.0 66 4277.5 67 3183.0 68 2638.5 69 2094.0 70 1822.5 71 1551.0 72 1276.0 73 1001.0 74 810.5 75 486.0 76 352.0 77 300.5 78 249.0 79 173.0 80 97.0 81 74.5 82 52.0 83 38.0 84 24.0 85 17.0 86 10.0 87 10.0 88 10.0 89 6.5 90 3.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 334858.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.03659166851334 #Duplication Level Percentage of deduplicated Percentage of total 1 74.39644245479275 30.529764306073805 2 9.370019428827913 7.690273224536952 3 4.0012042531082415 4.925873553513655 4 2.4335286947302266 3.9945489343701825 5 1.6430553718094179 3.3712696190850227 6 1.1871825071603301 2.9230754269404233 7 0.864785197094472 2.4841485909898475 8 0.6570584872134725 2.1570752673688287 9 0.5820297410559669 2.149606514037984 >10 4.78903568078113 36.144489213234046 >50 0.05237874237192897 1.473862538219087 >100 0.021096993455360282 1.5049100780154787 >500 0.002182447598830374 0.6511027336147112 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG 836 0.2496580640151945 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC 717 0.21412061231925175 No Hit GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC 628 0.18754218205926093 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 493 0.14722658559747714 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.061219979812338364 0.0 2 0.0 0.0 0.0 0.3604512957731337 0.0 3 0.0 0.0 0.0 0.5656128866564335 0.0 4 0.0 0.0 0.0 0.8905267307336244 0.0 5 0.0 0.0 0.0 1.9025975189483304 0.0 6 0.0 0.0 0.0 2.4708981120355493 0.0 7 0.0 0.0 0.0 3.0472618244151253 0.0 8 0.0 0.0 0.0 4.060228514773426 0.0 9 0.0 0.0 0.0 4.469655794396431 0.0 10 0.0 0.0 0.0 5.7024171439834195 0.0 11 0.0 0.0 0.0 6.969222775027027 0.0 12 0.0 0.0 0.0 8.253647814894672 0.0 13 0.0 0.0 0.0 8.632315787587574 0.0 14 0.0 0.0 0.0 8.795071343674035 0.0 15 0.0 0.0 0.0 9.108637093932353 0.0 16 0.0 0.0 0.0 9.8023639871229 0.0 17 0.0 0.0 0.0 10.60718274611925 0.0 18 0.0 0.0 0.0 11.515328885676913 0.0 19 0.0 0.0 0.0 11.974926685341249 0.0 20 0.0 0.0 0.0 12.494549928626462 0.0 21 0.0 0.0 0.0 13.122577331286694 0.0 22 0.0 0.0 0.0 13.816005590429375 0.0 23 0.0 0.0 0.0 14.50256526647116 0.0 24 0.0 0.0 0.0 15.018604901181993 0.0 25 0.0 0.0 0.0 15.486863088234415 0.0 26 0.0 0.0 0.0 15.921375627878085 0.0 27 0.0 0.0 0.0 16.352901827043105 0.0 28 0.0 0.0 0.0 16.802644703127893 0.0 29 0.0 0.0 0.0 17.283445520190647 0.0 30 0.0 0.0 0.0 17.76275316701408 0.0 31 0.0 0.0 0.0 18.25997885670941 0.0 32 0.0 0.0 0.0 18.737793333293517 0.0 33 0.0 0.0 0.0 19.165138655788425 0.0 34 0.0 0.0 0.0 19.621152846878378 0.0 35 0.0 0.0 0.0 20.120468974908768 0.0 36 0.0 0.0 0.0 20.595297111014222 0.0 37 0.0 0.0 0.0 21.08386241332147 0.0 38 0.0 0.0 0.0 21.53808480012423 0.0 39 0.0 0.0 0.0 21.982750897395313 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTCC 20 7.0261175E-4 45.000004 25 ACGCCGG 30 2.1608776E-6 45.000004 27 TCACCGG 20 7.0261175E-4 45.000004 2 TCGGGAA 40 6.7902874E-9 45.000004 5 GTTCGCT 20 7.0261175E-4 45.000004 21 TGCGATG 20 7.0261175E-4 45.000004 1 GTAGACG 30 2.1608776E-6 45.000004 1 TCGCTGC 20 7.0261175E-4 45.000004 21 CTGCCGA 20 7.0261175E-4 45.000004 42 ACTCGTT 20 7.0261175E-4 45.000004 23 TGTAGCG 20 7.0261175E-4 45.000004 1 TTACGCG 20 7.0261175E-4 45.000004 1 ATTATCG 20 7.0261175E-4 45.000004 30 TTCGCGC 20 7.0261175E-4 45.000004 34 TATCGCG 20 7.0261175E-4 45.000004 32 AGTACGT 20 7.0261175E-4 45.000004 39 GTAACGG 30 2.1608776E-6 45.000004 2 TCACGGA 25 3.8848655E-5 45.0 14 ATCCGCA 25 3.8848655E-5 45.0 11 GGCGATA 95 0.0 42.63158 8 >>END_MODULE