##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936260.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648412
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82739369413274	31.0	31.0	34.0	30.0	34.0
2	31.95512883783767	33.0	31.0	34.0	30.0	34.0
3	31.678762885325998	33.0	31.0	34.0	30.0	34.0
4	35.542668858688614	37.0	35.0	37.0	33.0	37.0
5	31.123003584140946	37.0	35.0	37.0	0.0	37.0
6	33.37120380252062	37.0	35.0	37.0	17.0	37.0
7	29.556733990117394	35.0	32.0	37.0	0.0	37.0
8	32.62284627674997	35.0	33.0	37.0	17.0	37.0
9	36.48489694823662	39.0	35.0	39.0	32.0	39.0
10	36.83423039672307	38.0	37.0	39.0	33.0	39.0
11	37.010366865511436	39.0	37.0	39.0	34.0	39.0
12	37.09486869459541	39.0	37.0	39.0	34.0	39.0
13	36.96209354546183	39.0	37.0	39.0	33.0	39.0
14	38.24469010443977	40.0	38.0	41.0	33.0	41.0
15	38.31665206689574	40.0	38.0	41.0	34.0	41.0
16	38.333918866399756	40.0	38.0	41.0	34.0	41.0
17	38.29410775864728	40.0	37.0	41.0	34.0	41.0
18	38.16997217818301	40.0	37.0	41.0	33.0	41.0
19	38.04818541297817	40.0	37.0	41.0	34.0	41.0
20	37.93440590242007	40.0	36.0	41.0	33.0	41.0
21	37.832279476629054	40.0	36.0	41.0	33.0	41.0
22	37.796445778301454	40.0	36.0	41.0	33.0	41.0
23	37.743112403842005	39.0	36.0	41.0	33.0	41.0
24	37.67745353263049	39.0	36.0	41.0	33.0	41.0
25	37.54221544326755	39.0	36.0	41.0	33.0	41.0
26	37.37704885165605	39.0	35.0	41.0	32.0	41.0
27	37.34736864832853	39.0	35.0	41.0	32.0	41.0
28	37.22720276614251	39.0	35.0	41.0	32.0	41.0
29	37.19329068555178	39.0	35.0	41.0	32.0	41.0
30	37.07265288119282	39.0	35.0	41.0	32.0	41.0
31	36.93120577657415	39.0	35.0	41.0	31.0	41.0
32	36.823251266170274	39.0	35.0	41.0	31.0	41.0
33	36.61000104871594	39.0	35.0	41.0	30.0	41.0
34	36.38730621888552	39.0	35.0	41.0	30.0	41.0
35	36.222114951604844	39.0	35.0	41.0	29.0	41.0
36	35.99138664922919	39.0	35.0	40.0	28.0	41.0
37	35.83437382405014	39.0	35.0	40.0	27.0	41.0
38	35.67260322140861	39.0	35.0	40.0	26.0	41.0
39	35.565822347519784	39.0	35.0	40.0	26.0	41.0
40	35.354012572253446	39.0	35.0	40.0	25.0	41.0
41	35.194342485950294	38.0	34.0	40.0	24.0	41.0
42	35.03656163056822	38.0	34.0	40.0	23.0	41.0
43	34.79928039579773	38.0	34.0	40.0	23.0	41.0
44	34.55128529391806	38.0	34.0	40.0	21.0	41.0
45	34.36340474883253	38.0	33.0	40.0	20.0	41.0
46	34.147261309167625	38.0	33.0	40.0	20.0	41.0
47	33.98948199601488	38.0	33.0	40.0	18.0	41.0
48	33.88769023398703	38.0	33.0	40.0	18.0	41.0
49	33.68593887836746	37.0	33.0	40.0	17.0	41.0
50	33.50915158880465	37.0	33.0	40.0	15.0	41.0
51	31.526492415316188	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	22.0
10	45.0
11	29.0
12	21.0
13	22.0
14	21.0
15	39.0
16	84.0
17	141.0
18	311.0
19	532.0
20	943.0
21	1565.0
22	2326.0
23	3454.0
24	5363.0
25	7674.0
26	10001.0
27	11123.0
28	12337.0
29	13327.0
30	15252.0
31	18962.0
32	23369.0
33	31246.0
34	43205.0
35	52902.0
36	63151.0
37	97469.0
38	119687.0
39	113756.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.491422120503636	21.548182328519523	25.18553018759678	14.774865363380075
2	33.197411522303724	24.64204857405477	25.365354126697227	16.795185776944287
3	30.808035631666286	24.10257675675342	28.87377161434397	16.215615997236323
4	27.046692534993184	25.990573894375796	28.817326021110034	18.145407549520982
5	21.206270087536936	39.09366267126457	23.353670197343664	16.346397043854832
6	24.818325385711553	33.939994941487825	26.68982066957428	14.551859003226344
7	67.51664065439876	19.147393940889433	9.165623091491213	4.170342313220607
8	81.35136302227596	4.8970407703743914	9.873660573832685	3.8779356335169615
9	74.6445161409721	7.947107703127024	11.91942777123187	5.488948384669007
10	35.9066457746001	32.31217189071146	18.25120448110152	13.529977853586917
11	27.244252111311944	26.13723990302462	28.950574634645875	17.667933351017563
12	26.8118110090498	22.224604109732702	31.565270229422033	19.398314651795463
13	23.434174568021565	23.391763261629954	32.57327131515147	20.600790855197005
14	19.34433662547886	26.823994620704124	31.32545356964399	22.506215184173026
15	19.100355946527824	26.221137178213848	34.5624386963844	20.116068178873924
16	21.838584110102836	25.694774310160824	32.649611666656384	19.817029913079956
17	21.767950007094257	25.017118745488983	30.81682633880928	22.39810490860749
18	22.184043478529084	26.04439769776007	30.96457190798443	20.806986915726423
19	22.958551044706144	27.36423755266713	28.67436136283721	21.002850039789518
20	24.348253887960126	26.370887645509338	29.529219076759837	19.751639389770702
21	23.708382941709903	26.757216091003865	29.764409048567884	19.769991918718347
22	22.761762583049048	24.69062879773971	29.325644806080085	23.221963813131158
23	21.74173210859762	26.580168164685414	30.198700826018026	21.479398900698936
24	21.41076969581069	25.419949044743156	31.71687137190552	21.45240988754064
25	21.615577749949104	27.703219557935387	28.74052300080813	21.94067969130738
26	21.188071781521625	27.986064415834377	28.98620013201483	21.839663670629168
27	21.827325836042515	27.22435735304097	29.52829373916584	21.420023071750677
28	20.296354786771374	27.319975571087518	30.95331363392411	21.430356008216997
29	20.999919804075187	25.218533895116067	31.448677692578176	22.33286860823057
30	21.67958026686736	25.7714231075304	30.85630740948656	21.692689216115678
31	21.34275738265177	27.23762052522162	29.52011992375218	21.89950216837443
32	22.812656150718986	26.80533364589181	28.908625997051256	21.473384206337943
33	21.85385218040382	26.245195955657824	28.508417487646742	23.392534376291618
34	20.572413835647705	26.607928292505385	30.740485987304368	22.079171884542546
35	21.5090097037069	24.734736556386988	30.281518540680924	23.474735199225183
36	20.50794864993245	27.262758863191923	29.17065075908527	23.058641727790356
37	21.628532476265093	26.981456234616264	29.592604701948762	21.797406587169885
38	21.374835752577066	26.961715699277622	27.98513907824038	23.67830946990494
39	21.41678439017168	25.057525153760263	28.875159620734962	24.65053083533309
40	21.651203247318062	24.92211741917176	30.61402318279119	22.812656150718986
41	20.007341011579058	25.267422564665676	30.306965324515893	24.418271099239373
42	21.162008105957323	26.006613079338447	29.320092780516095	23.51128603418814
43	21.50793014318057	25.82200822933567	29.191779300814915	23.478282326668847
44	21.69623634355934	26.371350314306337	28.416654842908525	23.5157584992258
45	21.417863950698013	25.339136228200587	28.890890359832945	24.352109461268455
46	21.263024126635536	26.76662368987619	28.468782194037125	23.501569989451152
47	20.792181514222438	25.70834592820614	30.2488232790263	23.250649278545122
48	20.83536393527572	25.130626823686175	29.977853586916957	24.056155654121145
49	21.027371486030486	25.27929773045533	29.613733243678404	24.079597539835785
50	19.453526461570732	25.088523963159226	30.79199644670364	24.665953128566407
51	19.54667711269995	24.407012825179052	29.502075840669207	26.544234221451795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	316.0
1	687.5
2	1059.0
3	4838.5
4	8618.0
5	6912.0
6	5206.0
7	4127.0
8	3048.0
9	2911.5
10	2775.0
11	2713.0
12	2651.0
13	2649.0
14	2647.0
15	2539.5
16	2432.0
17	2430.5
18	2429.0
19	2398.5
20	2368.0
21	2617.5
22	2867.0
23	3105.0
24	3343.0
25	4100.0
26	5696.5
27	6536.0
28	7852.5
29	9169.0
30	10984.0
31	12799.0
32	14709.5
33	16620.0
34	18523.0
35	20426.0
36	21876.5
37	23327.0
38	25802.0
39	28277.0
40	30861.5
41	33446.0
42	36090.5
43	38735.0
44	42020.5
45	45306.0
46	50020.5
47	54735.0
48	54750.5
49	54766.0
50	52005.0
51	49244.0
52	44784.0
53	40324.0
54	36441.0
55	32558.0
56	30409.0
57	28260.0
58	26544.5
59	24829.0
60	23822.0
61	22815.0
62	20893.5
63	18972.0
64	16149.0
65	13326.0
66	11145.0
67	8964.0
68	7589.0
69	6214.0
70	5401.0
71	4588.0
72	4145.5
73	3703.0
74	3052.5
75	1884.0
76	1366.0
77	1097.0
78	828.0
79	658.0
80	488.0
81	419.5
82	351.0
83	270.5
84	190.0
85	159.5
86	129.0
87	85.5
88	42.0
89	30.5
90	19.0
91	22.0
92	25.0
93	18.0
94	11.0
95	8.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.95783954210356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.540875863883	32.880306637727536
2	8.373031241420504	7.193746650999245
3	3.6410134773675566	4.692302181941761
4	2.1907071967499228	3.764321929668587
5	1.505389820482334	3.233414717829809
6	1.0899481786443042	2.8093091380446027
7	0.8627119313235398	2.5942168501798424
8	0.669280267793324	2.3000667482049373
9	0.5364953533879517	2.0742013175331397
>10	4.534388354406622	35.212747384843944
>50	0.04174927351583968	1.2147904435460615
>100	0.012931190663578252	0.9970359878643704
>500	7.389251807758995E-4	0.2277533655818801
>1k	7.389251807758995E-4	0.8057866460342894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3833	0.5911364996329495	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1244	0.1918533278224339	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	738	0.1138165240618619	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	697	0.1074933838362029	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08682751090356132	0.0
2	0.0	0.0	0.0	0.3109134315836228	0.0
3	0.0	0.0	0.0	0.4916627082780701	0.0
4	0.0	0.0	0.0	0.7001721127924838	0.0
5	0.0	0.0	0.0	1.364872951148344	0.0
6	0.0	0.0	0.0	2.0044354515339013	0.0
7	0.0	0.0	0.0	2.559946453797894	0.0
8	0.0	0.0	0.0	3.59354854629464	0.0
9	0.0	0.0	0.0	4.120682529009334	0.0
10	0.0	0.0	0.0	5.093675009099153	0.0
11	0.0	0.0	0.0	6.0404495906923374	0.0
12	0.0	0.0	0.0	6.850891100103021	0.0
13	0.0	0.0	0.0	7.246627144469874	0.0
14	0.0	0.0	0.0	7.4289186504876525	0.0
15	0.0	0.0	0.0	7.689709629063003	0.0
16	0.0	0.0	0.0	8.136030795235127	0.0
17	0.0	0.0	0.0	8.683213759153132	0.0
18	0.0	0.0	0.0	9.307353966305374	0.0
19	0.0	0.0	0.0	9.68119035428092	0.0
20	0.0	0.0	0.0	10.06597657045212	0.0
21	0.0	0.0	0.0	10.535893845271216	0.0
22	0.0	0.0	0.0	11.02431787197029	0.0
23	0.0	0.0	0.0	11.524462841526683	0.0
24	0.0	0.0	0.0	11.934387395668185	0.0
25	0.0	0.0	0.0	12.290179700560754	0.0
26	0.0	0.0	0.0	12.624535017859015	0.0
27	0.0	0.0	0.0	12.98510823365391	0.0
28	0.0	0.0	0.0	13.36835222050178	0.0
29	0.0	0.0	0.0	13.767481169379963	0.0
30	0.0	0.0	0.0	14.194061800213445	0.0
31	0.0	0.0	0.0	14.58594227127197	0.0
32	0.0	0.0	0.0	14.980444532180156	0.0
33	0.0	0.0	0.0	15.356440041208367	0.0
34	0.0	0.0	0.0	15.76096679271821	0.0
35	0.0	0.0	0.0	16.163642869040054	0.0
36	0.0	0.0	0.0	16.593616404384868	0.0
37	0.0	0.0	0.0	17.001381837473705	0.0
38	0.0	0.0	0.0	17.37768579236658	0.0
39	0.0	0.0	0.0	17.798560174703738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	45	3.8380676E-10	45.000004	1
CGCATCG	25	3.8889186E-5	45.0	21
TTCACGG	55	1.8189894E-12	45.0	2
CGCGATC	20	7.0310105E-4	45.0	39
GAATACG	20	7.0310105E-4	45.0	1
TAGTTCG	20	7.0310105E-4	45.0	1
ACGTATA	20	7.0310105E-4	45.0	32
ATCGCAT	20	7.0310105E-4	45.0	13
CGTTATT	390	0.0	42.692307	1
CGGTTTT	455	0.0	41.538464	1
CGTTTTT	1730	0.0	40.83815	1
ATAACGG	50	1.0804797E-9	40.5	2
CGTTTTA	250	0.0	40.499996	1
TACGGGT	45	1.9261279E-8	40.000004	4
TATCGCG	45	1.9261279E-8	40.000004	32
AACACGT	85	0.0	39.705883	41
CGGGTCA	40	3.4551158E-7	39.375	6
CACAACG	105	0.0	38.57143	12
TTAATCG	70	0.0	38.571426	20
TAACGCC	35	6.2440777E-6	38.571426	12
>>END_MODULE