##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	555521
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83557597282551	31.0	31.0	34.0	30.0	34.0
2	31.96538744709921	33.0	31.0	34.0	30.0	34.0
3	31.896473760667913	33.0	31.0	34.0	30.0	34.0
4	35.63894074211416	37.0	35.0	37.0	33.0	37.0
5	31.10142010833074	37.0	35.0	37.0	0.0	37.0
6	33.33590809348342	37.0	35.0	37.0	17.0	37.0
7	29.561498125183387	35.0	32.0	37.0	0.0	37.0
8	32.56456731608706	35.0	33.0	37.0	17.0	37.0
9	36.38494314346352	39.0	35.0	39.0	32.0	39.0
10	36.75900821031068	38.0	35.0	39.0	33.0	39.0
11	36.90621956685706	39.0	37.0	39.0	33.0	39.0
12	36.913306607671	39.0	35.0	39.0	33.0	39.0
13	36.696996153160725	39.0	35.0	39.0	33.0	39.0
14	37.95026470646474	40.0	37.0	41.0	33.0	41.0
15	38.03160816602793	40.0	37.0	41.0	33.0	41.0
16	38.07518167630027	40.0	37.0	41.0	33.0	41.0
17	37.98246690944177	40.0	37.0	41.0	33.0	41.0
18	37.88883228536815	40.0	36.0	41.0	33.0	41.0
19	37.811614682433245	40.0	36.0	41.0	33.0	41.0
20	37.75634764482351	40.0	36.0	41.0	33.0	41.0
21	37.66853458285105	39.0	36.0	41.0	33.0	41.0
22	37.63840070852407	39.0	36.0	41.0	33.0	41.0
23	37.61377337670403	39.0	36.0	41.0	33.0	41.0
24	37.530730611444035	39.0	36.0	41.0	33.0	41.0
25	37.401244957436354	39.0	35.0	41.0	32.0	41.0
26	37.244476806457364	39.0	35.0	41.0	32.0	41.0
27	37.19709965959883	39.0	35.0	41.0	32.0	41.0
28	37.061232608668256	39.0	35.0	41.0	32.0	41.0
29	37.02703408151987	39.0	35.0	41.0	31.0	41.0
30	36.87811621882881	39.0	35.0	41.0	31.0	41.0
31	36.77012570181865	39.0	35.0	41.0	31.0	41.0
32	36.65939001405887	39.0	35.0	40.0	30.0	41.0
33	36.5164449228742	39.0	35.0	40.0	30.0	41.0
34	36.3588001173673	39.0	35.0	40.0	30.0	41.0
35	36.23318110386466	39.0	35.0	40.0	30.0	41.0
36	36.12167136795909	39.0	35.0	40.0	29.0	41.0
37	36.028245556873635	39.0	35.0	40.0	29.0	41.0
38	35.923659051593006	38.0	35.0	40.0	29.0	41.0
39	35.93554699102284	38.0	35.0	40.0	29.0	41.0
40	35.78109378403337	38.0	35.0	40.0	28.0	41.0
41	35.701033804302625	38.0	35.0	40.0	28.0	41.0
42	35.64417186749016	38.0	35.0	40.0	27.0	41.0
43	35.491621378849764	38.0	34.0	40.0	27.0	41.0
44	35.34466923842663	38.0	34.0	40.0	26.0	41.0
45	35.24388996995613	38.0	34.0	40.0	26.0	41.0
46	35.091001060265945	38.0	34.0	40.0	26.0	41.0
47	34.931014309090024	38.0	34.0	40.0	25.0	41.0
48	34.85335387861125	38.0	34.0	40.0	24.0	41.0
49	34.70565289160986	37.0	34.0	40.0	24.0	41.0
50	34.539353147765794	37.0	33.0	40.0	24.0	41.0
51	32.483799892353304	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	17.0
9	22.0
10	48.0
11	18.0
12	12.0
13	17.0
14	19.0
15	40.0
16	82.0
17	126.0
18	262.0
19	527.0
20	847.0
21	1283.0
22	1931.0
23	2763.0
24	3705.0
25	4951.0
26	6186.0
27	7227.0
28	8543.0
29	10033.0
30	12742.0
31	15941.0
32	20757.0
33	29457.0
34	41565.0
35	49952.0
36	56203.0
37	83495.0
38	101986.0
39	94747.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74095848761793	20.965544056840336	24.471802146093488	11.821695309448248
2	30.224419958921445	26.45372542172123	25.896590767945764	17.425263851411557
3	31.521760653512647	25.5819311961204	26.377400674321944	16.518907476045012
4	27.974820033806104	26.59719434548829	27.108966177696253	18.319019443009353
5	22.19610059745716	39.230740152037455	21.385150156339723	17.188009094165658
6	25.019576217640736	35.861290572273596	25.095540942646632	14.023592267439033
7	69.31961167984649	19.260117979338315	7.2254694241981845	4.194800916617013
8	81.95963788947672	6.157643005394935	7.794124794562222	4.088594310566117
9	75.4381922555583	8.361520086549383	9.757326905733537	6.442960752158784
10	40.364990702421686	28.803591583396486	16.421161396238844	14.410256317942977
11	30.33008653138225	25.645115126160846	25.341796259727356	18.683002082729548
12	31.063092124330133	21.653186828220715	27.50571085521519	19.778010192233957
13	24.954052142043235	24.442460321031966	28.37192473371844	22.23156280320636
14	19.291439927563495	28.002181735703964	28.668403174677465	24.03797516205508
15	19.956221276963426	25.79146422907505	33.391176931205116	20.861137562756404
16	23.327830991087644	25.249270504625386	29.98068479859447	21.442213705692495
17	22.01698945674421	25.72197990715022	28.392806032535223	23.868224603570344
18	23.30856979304113	25.80244491207353	28.716826186588808	22.172159108296537
19	25.390759305228784	28.143670536307358	24.80338277040832	21.662187388055536
20	26.32609748326346	26.609975140453734	27.30211819175153	19.76180918453128
21	25.42082117507709	28.208114544724683	26.040419714106218	20.33064456609201
22	23.43907791064604	25.47734469083977	26.593234099160966	24.49034329935322
23	23.531783676944706	26.9048334806425	26.729322563863473	22.834060278549327
24	24.06317672959258	24.308531990689822	29.124371535909532	22.503919743808066
25	22.450996451979314	27.160269368754737	25.861128562196566	24.52760561706938
26	20.487434318414603	28.32656191215094	26.555611759051413	24.630392010383044
27	21.955245616277335	27.500670541707695	27.252795123856703	23.29128871815827
28	20.7603312926064	27.955018802169494	28.160051555206735	23.124598350017372
29	22.758275564740128	25.660056055486653	27.169269928589557	24.412398451183666
30	22.767456135771642	26.644717301416147	28.21171476865861	22.376111794153598
31	24.57476855060385	27.039121833378037	25.54268875524058	22.84342086077754
32	24.55964761008135	27.632258726492786	24.874667204300106	22.93342645912576
33	23.787219565056947	27.8939950064894	25.04567784116172	23.273107587291932
34	22.326248692668685	26.727702463093205	26.655877995611327	24.290170848626786
35	21.317645957578563	27.68158179438761	27.1672898054259	23.83348244260793
36	21.900162190088224	29.783212515818487	25.02551658713172	23.291108706961573
37	22.30680748342547	29.422830100032222	26.902493335085442	21.367869081456867
38	21.73725205707795	29.32202382988222	25.78732397155103	23.1534001414888
39	22.498339396710477	27.41048493216278	26.290995299907653	23.80018037121909
40	23.168341070814606	26.40944266733391	27.197711697667593	23.224504564183892
41	19.271098662336797	27.05730296424438	27.759706653753863	25.911891719664965
42	20.764291538933723	26.52861007954695	27.715783921759936	24.991314459759398
43	21.78819522574304	26.12268483099649	26.84596981932276	25.243150123937706
44	21.265442710536593	26.638596920728467	26.554891714264627	25.541068654470305
45	21.7347319003242	26.500168310468915	26.525369878006412	25.239729911200477
46	21.807816446182954	28.92761929792033	25.33405577826941	23.930508477627306
47	20.374387286889245	26.56407228529615	28.35608374840915	24.70545667940546
48	20.99920615062257	25.393279461982537	29.091069464520693	24.516444922874204
49	21.101092487952748	25.60965292041165	28.409007040237906	24.880247551397698
50	19.95046091866914	25.36681781606816	28.729066947964167	25.953654317298536
51	19.485852019995644	24.9153497347535	27.615157662806627	27.98364058244423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	584.5
2	955.0
3	2211.5
4	3468.0
5	2665.0
6	1862.0
7	1494.5
8	1127.0
9	1189.5
10	1252.0
11	1255.5
12	1259.0
13	1315.5
14	1372.0
15	1389.0
16	1406.0
17	1322.5
18	1239.0
19	1268.0
20	1297.0
21	1459.0
22	1621.0
23	1928.0
24	2235.0
25	2981.0
26	4488.0
27	5249.0
28	6268.5
29	7288.0
30	8924.0
31	10560.0
32	11910.5
33	13261.0
34	15699.5
35	18138.0
36	18784.0
37	19430.0
38	20175.5
39	20921.0
40	23946.0
41	26971.0
42	29235.0
43	31499.0
44	34575.0
45	37651.0
46	42641.5
47	47632.0
48	46444.5
49	45257.0
50	43549.5
51	41842.0
52	37571.5
53	33301.0
54	30675.0
55	28049.0
56	27939.0
57	27829.0
58	27036.0
59	26243.0
60	26139.0
61	26035.0
62	23217.0
63	20399.0
64	17438.5
65	14478.0
66	11954.0
67	9430.0
68	7950.5
69	6471.0
70	5558.0
71	4645.0
72	4024.5
73	3404.0
74	2918.5
75	2045.5
76	1658.0
77	1331.5
78	1005.0
79	786.5
80	568.0
81	458.0
82	348.0
83	293.5
84	239.0
85	159.0
86	79.0
87	69.0
88	59.0
89	59.5
90	60.0
91	49.5
92	39.0
93	26.0
94	13.0
95	7.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	555521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.34715539855982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69261032475424	27.522093695241107
2	8.964441232969468	6.695927595779358
3	4.014479893283596	4.497882132565689
4	2.5311146752912834	3.7811973243871533
5	1.7565494859214457	3.2801063307984304
6	1.3131887230844468	2.942631798520271
7	0.9508869408812283	2.485904564328668
8	0.7522027037419052	2.247410501429263
9	0.5821874187453524	1.9568739599072187
>10	5.3359689435794655	39.747868702525544
>50	0.07666281639503035	1.7640509724529863
>100	0.02683198573778856	1.8293663107240274
>500	4.7914260246051E-4	0.1313464611240767
>1k	0.00239571301230255	1.11733965021626
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1442	0.2595761456362586	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	1279	0.23023432057473975	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	1274	0.22933426459125758	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1199	0.21583342483902498	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	1050	0.18901175653125624	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	734	0.13212821837518293	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08460526244732422	0.0
2	0.0	0.0	0.0	0.34958174398447583	0.0
3	0.0	0.0	0.0	0.4887303990308197	0.0
4	0.0	0.0	0.0	0.7351657273082386	0.0
5	0.0	0.0	0.0	1.571317735963177	0.0
6	0.0	0.0	0.0	2.131872602475874	0.0
7	0.0	0.0	0.0	2.6693860358114274	0.0
8	0.0	0.0	0.0	3.686629308342979	0.0
9	0.0	0.0	0.0	4.15663854291737	0.0
10	0.0	0.0	0.0	5.28854894774455	0.0
11	0.0	0.0	0.0	6.248008626136546	0.0
12	0.0	0.0	0.0	7.230869760099078	0.0
13	0.0	0.0	0.0	7.597372556573019	0.0
14	0.0	0.0	0.0	7.7662230590742745	0.0
15	0.0	0.0	0.0	8.02345905915348	0.0
16	1.8001119669643453E-4	0.0	0.0	8.475647185254923	0.0
17	1.8001119669643453E-4	0.0	0.0	9.022701212015388	0.0
18	1.8001119669643453E-4	0.0	0.0	9.624478642571567	0.0
19	1.8001119669643453E-4	0.0	0.0	10.002862178027474	0.0
20	1.8001119669643453E-4	0.0	0.0	10.394386530842219	0.0
21	1.8001119669643453E-4	0.0	0.0	10.864395765416608	0.0
22	1.8001119669643453E-4	0.0	0.0	11.360866645905375	0.0
23	1.8001119669643453E-4	0.0	0.0	11.84383668664191	0.0
24	1.8001119669643453E-4	0.0	0.0	12.236981140226922	0.0
25	1.8001119669643453E-4	0.0	0.0	12.607984216618274	0.0
26	1.8001119669643453E-4	0.0	0.0	12.933984493835517	0.0
27	1.8001119669643453E-4	0.0	0.0	13.276005767558742	0.0
28	1.8001119669643453E-4	0.0	0.0	13.624687455559735	0.0
29	1.8001119669643453E-4	0.0	0.0	13.967428774069747	0.0
30	1.8001119669643453E-4	0.0	0.0	14.393515276650207	0.0
31	3.6002239339286905E-4	0.0	0.0	14.778559226383882	0.0
32	3.6002239339286905E-4	0.0	0.0	15.174223836722645	0.0
33	3.6002239339286905E-4	0.0	0.0	15.535506308492389	0.0
34	3.6002239339286905E-4	0.0	0.0	15.897148802655526	0.0
35	3.6002239339286905E-4	0.0	0.0	16.294973547354644	0.0
36	3.6002239339286905E-4	0.0	0.0	16.69405837043064	0.0
37	3.6002239339286905E-4	0.0	0.0	17.078562286574225	0.0
38	3.6002239339286905E-4	0.0	0.0	17.486107635894953	0.0
39	3.6002239339286905E-4	0.0	0.0	17.871871630415413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	45	3.8380676E-10	45.000004	1
CACGTTG	30	2.1634678E-6	45.000004	1
TCGCACC	25	3.8881946E-5	45.0	45
CGCGACT	20	7.030137E-4	45.0	14
TCATCGA	50	2.1827873E-11	45.0	16
TATACGA	25	3.8881946E-5	45.0	41
CGTTGAT	105	0.0	42.857143	25
CGTTATT	85	0.0	42.35294	1
GTACCCG	40	3.4539153E-7	39.375	43
CGTTTTA	80	0.0	39.375	1
TCGTTGA	115	0.0	39.130436	24
AGCCGTT	70	0.0	38.57143	45
ATAACGG	35	6.2424606E-6	38.57143	2
GGCCTAT	140	0.0	38.57143	8
AGGCGAT	95	0.0	37.894737	7
CTCAACC	120	0.0	37.500004	36
GTTTACG	30	1.1391626E-4	37.500004	1
GACGTCC	30	1.1391626E-4	37.500004	10
GTTAGCG	30	1.1391626E-4	37.500004	1
ACGGGCC	90	0.0	37.500004	5
>>END_MODULE