FastQCFastQC Report
Sat 14 Jan 2017
SRR2936228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998909
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52340.5239716530735031No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.13985257916386779No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.10721697371832668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTC207.0328417E-445.00000421
TATAGCG351.2117198E-745.0000041
ATACGCG207.0328417E-445.0000041
CGTTATT4550.043.0219761
TACGAAT650.041.5384612
CGTTTTT21000.039.9642871
TACGGGA2850.039.4736824
ACGGGAC2250.039.05
ATAACGG1100.038.8636362
ACGACCA7550.038.7417228
CACGGGA3250.038.0769234
CACGACC7750.038.03225727
AGACACG7650.037.94117424
AGCGACT3350.037.6119419
CTAGACG301.1398149E-437.4999961
AACACGT7350.037.3469441
TACGGGT1450.037.241384
ACACGAC7800.037.21153626
ACGGGAT3650.036.98635
CGGTTTT5400.036.6666681
Produced by FastQC (version 0.11.5)