##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	998909
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00324053542415	33.0	31.0	34.0	30.0	34.0
2	32.14717456745309	33.0	31.0	34.0	30.0	34.0
3	31.959408714907966	33.0	31.0	34.0	30.0	34.0
4	35.7432398747033	37.0	35.0	37.0	35.0	37.0
5	31.222658920882683	37.0	35.0	37.0	0.0	37.0
6	33.462994126592115	37.0	35.0	37.0	17.0	37.0
7	29.600993684109362	35.0	32.0	37.0	0.0	37.0
8	32.69320528696808	35.0	35.0	37.0	17.0	37.0
9	36.63328191056443	39.0	35.0	39.0	32.0	39.0
10	36.946575714104085	38.0	37.0	39.0	34.0	39.0
11	37.024716966210136	39.0	37.0	39.0	34.0	39.0
12	36.97947060242725	39.0	37.0	39.0	33.0	39.0
13	36.81208198144175	39.0	37.0	39.0	33.0	39.0
14	37.98770959116396	40.0	37.0	41.0	33.0	41.0
15	38.123066265295435	40.0	37.0	41.0	33.0	41.0
16	38.204302894457854	40.0	37.0	41.0	33.0	41.0
17	38.18149701324145	40.0	37.0	41.0	33.0	41.0
18	38.090365588857445	40.0	37.0	41.0	33.0	41.0
19	38.01440471554466	40.0	37.0	41.0	33.0	41.0
20	37.893291581114994	40.0	36.0	41.0	33.0	41.0
21	37.78963248904555	40.0	36.0	41.0	33.0	41.0
22	37.785659154137164	40.0	36.0	41.0	33.0	41.0
23	37.72259535152852	39.0	36.0	41.0	33.0	41.0
24	37.664903409619896	40.0	36.0	41.0	33.0	41.0
25	37.50026879325344	39.0	35.0	41.0	33.0	41.0
26	37.367492934791855	39.0	35.0	41.0	33.0	41.0
27	37.32645015712142	39.0	35.0	41.0	33.0	41.0
28	37.2172580285091	39.0	35.0	41.0	32.0	41.0
29	37.14643375923132	39.0	35.0	41.0	32.0	41.0
30	36.99693966117034	39.0	35.0	41.0	31.0	41.0
31	36.862189648906956	39.0	35.0	41.0	31.0	41.0
32	36.72194864597276	39.0	35.0	41.0	31.0	41.0
33	36.51198958063247	39.0	35.0	41.0	30.0	41.0
34	36.30753952562245	39.0	35.0	40.0	30.0	41.0
35	36.091579913685834	39.0	35.0	40.0	29.0	41.0
36	35.906500992582906	39.0	35.0	40.0	29.0	41.0
37	35.800008809611285	39.0	35.0	40.0	28.0	41.0
38	35.519488762239604	38.0	35.0	40.0	26.0	41.0
39	35.416238115784324	38.0	34.0	40.0	26.0	41.0
40	35.21307546533268	38.0	34.0	40.0	24.0	41.0
41	35.01759019089827	38.0	34.0	40.0	24.0	41.0
42	34.98679259071647	38.0	34.0	40.0	24.0	41.0
43	34.86310264498568	38.0	34.0	40.0	23.0	41.0
44	34.65833023828998	38.0	34.0	40.0	23.0	41.0
45	34.4668363184234	38.0	33.0	40.0	23.0	41.0
46	34.353890094092655	37.0	33.0	40.0	23.0	41.0
47	34.167691951919544	37.0	33.0	40.0	22.0	41.0
48	34.059580001781946	37.0	33.0	40.0	22.0	41.0
49	33.92251446327944	37.0	33.0	40.0	21.0	41.0
50	33.73624624465292	37.0	33.0	40.0	20.0	41.0
51	31.786198742828425	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	26.0
9	52.0
10	44.0
11	37.0
12	24.0
13	35.0
14	35.0
15	61.0
16	111.0
17	284.0
18	520.0
19	883.0
20	1447.0
21	2368.0
22	3711.0
23	5483.0
24	8190.0
25	11257.0
26	14032.0
27	15760.0
28	17516.0
29	19229.0
30	22678.0
31	27949.0
32	36065.0
33	50855.0
34	72977.0
35	84602.0
36	100430.0
37	153713.0
38	182954.0
39	165567.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94874808415982	19.52500177693864	23.67112519759057	12.855124941310969
2	34.937316612424155	23.00039342923129	24.66941433103516	17.392875627309394
3	33.0744842623302	23.18699701374199	27.129298064188028	16.609220659739776
4	28.077132151176937	25.695834155063174	27.167840113563894	19.059193580195995
5	22.058065349296083	39.24641784186548	22.554206639443635	16.14131016939481
6	26.33933621581145	35.098792782926175	25.080763112555797	13.481107888706578
7	72.21658829783294	18.39306683591799	6.476966370310008	2.9133784959390696
8	87.41707202557991	3.1667549296282247	7.138287872068427	2.2778851727234413
9	81.98264306358237	5.233409649928071	8.864971684107362	3.9189756023821993
10	44.37861707122471	26.228014764107645	16.284866789667525	13.108501375000126
11	32.44549803835985	25.930490164769765	24.559294189961246	17.064717606909138
12	28.306482372268142	23.863334898374124	28.990929103652086	18.839253625705645
13	25.27587598069494	23.832301040435112	31.116948590912685	19.77487438795726
14	22.201421751130486	24.713762715122197	31.725912971051418	21.3589025626959
15	21.882073342016138	25.154043060979532	31.962871492798644	21.00101210420569
16	26.760395591590424	25.511633191812265	28.89162075824725	18.83635045835006
17	26.48809851548039	24.72897931643423	27.83046303517137	20.952459132914008
18	26.552869180275678	24.26427232110232	28.925357565103525	20.257500933518468
19	25.491911675638125	25.914572798923626	27.09295841763364	21.500557107804617
20	26.789427265146276	26.97462932058876	26.890137139619323	19.34580627464564
21	26.93318410385731	25.363972093554064	28.715528641748143	18.987315160840478
22	26.85610000510557	22.548900850828254	29.014154442496764	21.58084470156941
23	24.612251966895883	25.263362328300175	29.62081631059486	20.50356939420908
24	23.864035662908233	24.6081474889104	30.512188797978595	21.01562805020277
25	24.70054829819333	25.82317308183228	28.59920172908643	20.877076890887956
26	23.80617253423485	26.37677706377658	27.486487758144136	22.330562643844434
27	23.56150560261245	25.64908314971634	29.03287486647933	21.75653638119188
28	22.012615763798305	26.80164058988356	28.82705031189027	22.358693334427862
29	23.031827724046934	25.821671443544908	28.969806058409723	22.17669477399843
30	24.45538082047514	24.54137463973195	29.39136598028449	21.611878559508423
31	25.635668514349153	24.736187180213612	27.939982520930336	21.688161784506896
32	25.99355897283937	24.272981823169076	27.66298031151987	22.070478892471687
33	24.98405760684907	24.151249012672828	28.282656378108516	22.582037002369585
34	23.133038144615774	25.00207726629753	30.48035406628632	21.384530522800375
35	23.812179087384337	25.83298378530977	29.207565453910217	21.14727167339567
36	25.51914138324912	26.44915602922789	27.663981403711453	20.36772118381154
37	24.52005137605127	26.44565220655735	27.187261302080568	21.847035115310803
38	24.3350495390471	26.62144399539898	27.994041499275713	21.049464966278208
39	24.62566660226307	24.997071805339626	27.60171346939511	22.775548123002196
40	26.057228436223923	24.857419444614074	28.24441465639012	20.840937462771883
41	22.70947603835785	24.50964001725883	29.160414011686754	23.620469932696572
42	23.215428031982892	24.936305509310657	28.69480603338242	23.15346042532403
43	23.649902043129053	23.977859845090993	29.098446405027882	23.273791706752068
44	23.935113208510487	24.807264725815863	28.403087768755714	22.85453429691794
45	23.34306728640947	24.55969462683788	27.605017073627323	24.49222101312532
46	23.376703984046596	24.89145657912783	28.047199494648662	23.684639942176915
47	22.71157833196017	25.186278229548435	29.631127560168142	22.47101587832325
48	23.207018857573615	25.182574188439588	29.401977557515245	22.20842939647155
49	23.873345820289938	24.85541726023091	29.060204683309493	22.21103223616966
50	22.925511733301033	25.68992771113285	28.92585811119932	22.458702444366804
51	22.81489104613133	24.994869402518148	28.392476191524953	23.79776335982557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	912.0
2	1499.0
3	6037.5
4	10576.0
5	7733.0
6	4890.0
7	3916.0
8	2942.0
9	2854.5
10	2767.0
11	2774.5
12	2782.0
13	2841.5
14	2901.0
15	2715.5
16	2530.0
17	2652.0
18	2774.0
19	2736.5
20	2699.0
21	2994.0
22	3289.0
23	3805.5
24	4322.0
25	5607.0
26	7569.0
27	8246.0
28	10259.5
29	12273.0
30	14256.5
31	16240.0
32	18428.0
33	20616.0
34	23856.0
35	27096.0
36	29182.5
37	31269.0
38	33808.5
39	36348.0
40	40209.0
41	44070.0
42	47136.0
43	50202.0
44	53842.5
45	57483.0
46	62882.5
47	68282.0
48	71133.5
49	73985.0
50	75635.5
51	77286.0
52	73826.0
53	70366.0
54	67374.0
55	64382.0
56	62131.0
57	59880.0
58	57111.0
59	54342.0
60	49444.5
61	44547.0
62	40882.0
63	37217.0
64	33414.0
65	29611.0
66	25554.5
67	21498.0
68	18501.0
69	15504.0
70	12883.5
71	10263.0
72	8508.5
73	6754.0
74	5618.0
75	3542.5
76	2603.0
77	1918.0
78	1233.0
79	1023.0
80	813.0
81	583.5
82	354.0
83	297.5
84	241.0
85	176.5
86	112.0
87	87.0
88	62.0
89	45.0
90	28.0
91	24.0
92	20.0
93	14.0
94	8.0
95	5.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	998909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.55618011078598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.52573595132078	24.336866582498264
2	8.051007239117137	5.4032209797811275
3	3.9225013409280285	3.948724844429414
4	2.695702090165305	3.6182985945043677
5	1.9103947620408865	3.2052775358873054
6	1.4214354825194704	2.86187670403712
7	1.1166765141694683	2.6229978764468735
8	0.8901949368587712	2.389723330795414
9	0.665194955615574	2.008926156548024
>10	6.67798138429996	45.20717674008474
>50	0.10199059269575948	2.119964271260449
>100	0.018763635952620976	1.1606627813399035
>500	0.001513196447622978	0.3341132689095032
>1k	6.052785790491912E-4	0.25063572877466683
>5k	3.026392895245956E-4	0.5315346047028389
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5234	0.5239716530735031	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1397	0.13985257916386779	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.10721697371832668	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0010921915810148E-4	0.0	0.0	0.05015471879820885	0.0
2	1.0010921915810148E-4	0.0	0.0	0.1750910243075195	0.0
3	1.0010921915810148E-4	0.0	0.0	0.27339827752077517	0.0
4	1.0010921915810148E-4	0.0	0.0	0.3723061860489794	0.0
5	1.0010921915810148E-4	0.0	0.0	0.7296960984434018	0.0
6	1.0010921915810148E-4	0.0	0.0	1.1082090560801834	0.0
7	1.0010921915810148E-4	0.0	0.0	1.390717272544346	0.0
8	1.0010921915810148E-4	0.0	0.0	1.8294959801143047	0.0
9	1.0010921915810148E-4	0.0	0.0	2.07336203798344	0.0
10	1.0010921915810148E-4	0.0	0.0	2.4998273115969525	0.0
11	1.0010921915810148E-4	0.0	0.0	2.996969693936084	0.0
12	1.0010921915810148E-4	0.0	0.0	3.339743660333424	0.0
13	1.0010921915810148E-4	0.0	0.0	3.512331954161991	0.0
14	1.0010921915810148E-4	0.0	0.0	3.625955917906436	0.0
15	1.0010921915810148E-4	0.0	0.0	3.752894407798909	0.0
16	1.0010921915810148E-4	0.0	0.0	3.9886516189162378	0.0
17	1.0010921915810148E-4	0.0	0.0	4.265753937545862	0.0
18	1.0010921915810148E-4	0.0	0.0	4.567883560965013	0.0
19	1.0010921915810148E-4	0.0	0.0	4.764397958172366	0.0
20	1.0010921915810148E-4	0.0	0.0	4.971624041829636	0.0
21	1.0010921915810148E-4	0.0	0.0	5.222097308163206	0.0
22	1.0010921915810148E-4	0.0	0.0	5.4944944934924	0.0
23	1.0010921915810148E-4	0.0	0.0	5.770195283053812	0.0
24	1.0010921915810148E-4	0.0	0.0	5.989834909886686	0.0
25	1.0010921915810148E-4	0.0	0.0	6.2115768303218815	0.0
26	1.0010921915810148E-4	0.0	0.0	6.41099439488482	0.0
27	1.0010921915810148E-4	0.0	0.0	6.652057394617528	0.0
28	1.0010921915810148E-4	0.0	1.0010921915810148E-4	6.867692652684078	0.0
29	1.0010921915810148E-4	0.0	1.0010921915810148E-4	7.103549973020566	0.0
30	1.0010921915810148E-4	0.0	1.0010921915810148E-4	7.45763628118277	0.0
31	1.0010921915810148E-4	0.0	1.0010921915810148E-4	7.719622107719522	0.0
32	1.0010921915810148E-4	0.0	1.0010921915810148E-4	7.991919183829558	0.0
33	1.0010921915810148E-4	0.0	1.0010921915810148E-4	8.257909379132634	0.0
34	1.0010921915810148E-4	0.0	1.0010921915810148E-4	8.510184611411049	0.0
35	1.0010921915810148E-4	0.0	1.0010921915810148E-4	8.782481687521086	0.0
36	1.0010921915810148E-4	0.0	1.0010921915810148E-4	9.010930925639872	0.0
37	1.0010921915810148E-4	0.0	1.0010921915810148E-4	9.257600041645436	0.0
38	1.0010921915810148E-4	0.0	1.0010921915810148E-4	9.561831958666906	0.0
39	1.0010921915810148E-4	0.0	1.0010921915810148E-4	10.011522571125097	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGTC	20	7.0328417E-4	45.000004	21
TATAGCG	35	1.2117198E-7	45.000004	1
ATACGCG	20	7.0328417E-4	45.000004	1
CGTTATT	455	0.0	43.021976	1
TACGAAT	65	0.0	41.53846	12
CGTTTTT	2100	0.0	39.964287	1
TACGGGA	285	0.0	39.473682	4
ACGGGAC	225	0.0	39.0	5
ATAACGG	110	0.0	38.863636	2
ACGACCA	755	0.0	38.74172	28
CACGGGA	325	0.0	38.076923	4
CACGACC	775	0.0	38.032257	27
AGACACG	765	0.0	37.941174	24
AGCGACT	335	0.0	37.61194	19
CTAGACG	30	1.1398149E-4	37.499996	1
AACACGT	735	0.0	37.34694	41
TACGGGT	145	0.0	37.24138	4
ACACGAC	780	0.0	37.211536	26
ACGGGAT	365	0.0	36.9863	5
CGGTTTT	540	0.0	36.666668	1
>>END_MODULE