##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936227.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	640969
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98983570188262	33.0	31.0	34.0	30.0	34.0
2	32.12429618281071	33.0	31.0	34.0	30.0	34.0
3	32.046958589260946	33.0	31.0	34.0	30.0	34.0
4	35.779087600180354	37.0	35.0	37.0	35.0	37.0
5	31.28947577807975	37.0	35.0	37.0	0.0	37.0
6	33.47720716602519	37.0	35.0	37.0	17.0	37.0
7	29.436220784468517	35.0	32.0	37.0	0.0	37.0
8	32.554189048144295	35.0	33.0	37.0	17.0	37.0
9	36.47051573477032	39.0	35.0	39.0	32.0	39.0
10	36.80280793610923	38.0	35.0	39.0	33.0	39.0
11	36.913782101786516	39.0	37.0	39.0	33.0	39.0
12	36.90718739907858	39.0	35.0	39.0	33.0	39.0
13	36.76996703428715	39.0	35.0	39.0	33.0	39.0
14	38.04091149493969	40.0	37.0	41.0	33.0	41.0
15	38.139059767321044	40.0	37.0	41.0	33.0	41.0
16	38.19104824102258	40.0	37.0	41.0	33.0	41.0
17	38.15091681500977	40.0	37.0	41.0	33.0	41.0
18	38.076279820084906	40.0	37.0	41.0	33.0	41.0
19	38.01897439657768	40.0	37.0	41.0	33.0	41.0
20	37.93214024391195	40.0	36.0	41.0	33.0	41.0
21	37.86631178730953	40.0	36.0	41.0	33.0	41.0
22	37.82175425020555	40.0	36.0	41.0	33.0	41.0
23	37.775970444748495	40.0	36.0	41.0	33.0	41.0
24	37.72316913922514	40.0	36.0	41.0	33.0	41.0
25	37.586518224750336	39.0	36.0	41.0	33.0	41.0
26	37.418847089328814	39.0	36.0	41.0	32.0	41.0
27	37.389527418642714	39.0	36.0	41.0	32.0	41.0
28	37.23533119386429	39.0	35.0	41.0	32.0	41.0
29	37.2008771095014	39.0	35.0	41.0	32.0	41.0
30	37.1063623981815	39.0	35.0	41.0	32.0	41.0
31	37.012133192088854	39.0	35.0	41.0	31.0	41.0
32	36.94655747781874	39.0	35.0	41.0	31.0	41.0
33	36.81464470200587	39.0	35.0	41.0	31.0	41.0
34	36.69820225315109	39.0	35.0	41.0	30.0	41.0
35	36.61168792874538	39.0	35.0	41.0	30.0	41.0
36	36.48969918982041	39.0	35.0	41.0	30.0	41.0
37	36.427633785721305	39.0	35.0	40.0	30.0	41.0
38	36.31585458891148	39.0	35.0	40.0	30.0	41.0
39	36.2923838750392	39.0	35.0	40.0	30.0	41.0
40	36.15055018261413	39.0	35.0	40.0	29.0	41.0
41	36.02222884414067	39.0	35.0	40.0	29.0	41.0
42	36.001446247790454	38.0	35.0	40.0	29.0	41.0
43	35.86151436340915	38.0	35.0	40.0	29.0	41.0
44	35.74326059450613	38.0	35.0	40.0	28.0	41.0
45	35.62374623421726	38.0	34.0	40.0	28.0	41.0
46	35.52728447085585	38.0	34.0	40.0	27.0	41.0
47	35.40503050849573	38.0	34.0	40.0	27.0	41.0
48	35.29283163460323	38.0	34.0	40.0	27.0	41.0
49	35.13017790251946	38.0	34.0	40.0	26.0	41.0
50	34.95613360396525	38.0	34.0	40.0	26.0	41.0
51	32.95079793250532	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	28.0
10	25.0
11	24.0
12	12.0
13	14.0
14	11.0
15	33.0
16	66.0
17	139.0
18	241.0
19	514.0
20	950.0
21	1459.0
22	2132.0
23	2932.0
24	3775.0
25	4851.0
26	5931.0
27	7121.0
28	8454.0
29	10170.0
30	12714.0
31	16685.0
32	21884.0
33	31948.0
34	47025.0
35	55571.0
36	64653.0
37	100599.0
38	124026.0
39	116958.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31389193549142	19.92920094419543	23.497392229577404	11.259514890735746
2	33.27508818679218	23.836254171418584	24.770308704477127	18.1183489373121
3	33.51862570576736	24.07433120790553	25.932767419329174	16.474275666997936
4	28.862394281158682	26.495977184544028	25.86178114698215	18.779847387315144
5	22.458808460315556	40.15155179111626	20.34341754437422	17.04622220419396
6	27.227369810396446	36.885715221797	22.797514388371358	13.089400579435198
7	72.53782944260955	19.33057604969975	5.130045290801895	3.0015492168888045
8	86.81933135611862	4.582124876554093	5.8478647173264235	2.750679050000858
9	80.52089882662032	7.091138573004311	7.788988235000445	4.598974365374924
10	43.5869753451415	28.39622509044899	14.987932333701007	13.028867230708505
11	31.55300802378898	27.55702693890032	23.337946140921012	17.552018896389683
12	30.195688090999724	24.015514010817995	27.163560172176815	18.62523772600547
13	25.50965803338383	24.982175425020557	27.964534946307857	21.54363159528776
14	20.905847240662183	27.344848190786138	28.808881552774	22.940423015777675
15	20.6061447589509	27.889648329326377	31.578594284590988	19.925612627131734
16	24.273560811833335	26.246511141724483	29.750892788886823	19.729035257555356
17	23.898035630428303	26.238398424884824	27.402417277590647	22.461148667096225
18	24.876086050963465	26.665564169249993	27.542049615504027	20.916300164282518
19	25.29373495442057	27.931460023807702	26.452137310852784	20.32266771091894
20	26.976811671079254	26.28239431236144	27.659059954537586	19.08173406202172
21	27.41443033906476	26.085660928999687	27.05325842591451	19.44665030602104
22	25.92574679898716	24.88139051966632	26.632177219179088	22.560685462167438
23	24.410072874039148	26.36430154968493	27.764993314809296	21.46063226146662
24	23.72345620459024	25.24271844660194	29.295020508012087	21.738804840795733
25	23.28287327468255	27.02923230296629	27.565607697096116	22.122286725255044
26	22.778324692769853	27.76436925966778	27.65952799589372	21.797778051668644
27	22.739321246425334	28.229134326309076	27.465134819312638	21.56640960795296
28	21.22411536283346	27.87591911621311	28.687190800179103	22.212774720774327
29	22.449447633192868	26.39160396212609	28.708408674990523	22.450539729690515
30	24.10100956520518	26.365237632397196	28.011339081921278	21.52241372047634
31	24.328633678071796	26.59239370390768	26.826570395760168	22.252402222260358
32	24.770932759618642	26.89708862675106	27.547822125563016	20.784156488067286
33	24.560314149358238	26.463526317185387	26.375534542232153	22.600624991224226
34	22.437122544148	26.71127620836577	28.350513051333216	22.501088196153013
35	23.45324032831541	25.296855230128134	28.532425125084053	22.717479316472403
36	23.608006003410463	28.257372821462507	26.908945674439792	21.22567550068724
37	23.380537904329227	27.165120310030595	28.364866319588	21.08947546605218
38	23.114534400259608	26.712524318648796	27.5635795178862	22.609361763205396
39	23.588348266452826	26.208599791877614	27.75095207412527	22.452099867544295
40	24.37933815831967	25.093881295351256	28.753652672750164	21.77312787357891
41	21.42849342167874	25.160655195493074	29.940761565691943	23.47008981713624
42	22.09654445066766	25.60919482845504	27.626765100964324	24.667495619912973
43	22.551012607473996	25.22898923348867	28.772374326995532	23.4476238320418
44	22.28953350318034	25.019462719725915	28.228666284952936	24.462337492140808
45	22.75757485931457	25.476115069527545	28.157367985035158	23.60894208612273
46	22.876145336201905	26.189566110061484	27.48338843220187	23.45090012153474
47	22.004808344865353	24.994968555421558	29.87929213425298	23.120930965460108
48	22.12415889067958	24.480279077459286	30.394293639786014	23.001268392075126
49	22.30513488171815	25.227585109420268	29.139630777775526	23.32764923108606
50	20.74172073844445	24.99247233485551	30.591183036933145	23.6746238897669
51	20.90116682710084	25.456925373926044	28.814498049047614	24.827409749925504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	200.0
1	733.5
2	1267.0
3	1971.5
4	2676.0
5	1994.0
6	1312.0
7	1138.5
8	965.0
9	904.5
10	844.0
11	923.0
12	1002.0
13	1060.5
14	1119.0
15	1108.5
16	1098.0
17	1172.0
18	1246.0
19	1391.0
20	1536.0
21	1981.0
22	2426.0
23	2512.5
24	2599.0
25	3434.0
26	5189.5
27	6110.0
28	7699.5
29	9289.0
30	10934.5
31	12580.0
32	14521.0
33	16462.0
34	18846.0
35	21230.0
36	21778.0
37	22326.0
38	24333.5
39	26341.0
40	29241.5
41	32142.0
42	35268.5
43	38395.0
44	41224.5
45	44054.0
46	46752.0
47	49450.0
48	49057.5
49	48665.0
50	47935.0
51	47205.0
52	43686.0
53	40167.0
54	37721.0
55	35275.0
56	34353.5
57	33432.0
58	32491.0
59	31550.0
60	30778.5
61	30007.0
62	26796.5
63	23586.0
64	20124.5
65	16663.0
66	14163.0
67	11663.0
68	9885.0
69	8107.0
70	6638.5
71	5170.0
72	4330.5
73	3491.0
74	2783.5
75	1749.0
76	1422.0
77	1055.0
78	688.0
79	560.5
80	433.0
81	338.0
82	243.0
83	167.5
84	92.0
85	64.5
86	37.0
87	33.0
88	29.0
89	22.5
90	16.0
91	12.5
92	9.0
93	6.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	640969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.81562615935062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28417475828668	23.39234829945754
2	8.427870106485743	5.531316694680053
3	4.073028871988855	4.009769783982836
4	2.673829127859778	3.5097350829531595
5	2.0480310272854547	3.3603710277075156
6	1.5294966337237668	3.011483384655862
7	1.1460532636634835	2.6325918821359195
8	0.9305948827198041	2.4430443021710264
9	0.7399878172044372	2.185484721466923
>10	7.043214969093402	46.567320155306554
>50	0.07743043204537473	1.6155283670787883
>100	0.024854212753603874	1.3993926301224804
>500	9.559312597539949E-4	0.1739437824261187
>1k	4.7796562987699747E-4	0.16766988585529385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1069	0.16677873656916326	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06490173471727961	0.0
2	0.0	0.0	0.0	0.2859732685980133	0.0
3	0.0	0.0	0.0	0.408444090119803	0.0
4	0.0	0.0	0.0	0.5497925796723399	0.0
5	0.0	0.0	0.0	0.9994243091319549	0.0
6	0.0	0.0	0.0	1.3311096168457446	0.0
7	0.0	0.0	0.0	1.5919646659978877	0.0
8	0.0	0.0	0.0	1.9983805769077756	0.0
9	0.0	0.0	0.0	2.155174431212742	0.0
10	0.0	0.0	0.0	2.631640531757386	0.0
11	0.0	0.0	0.0	3.261624197114057	0.0
12	0.0	0.0	0.0	3.71952465719871	0.0
13	0.0	0.0	0.0	3.9387240256549068	0.0
14	0.0	0.0	0.0	4.045281441068132	0.0
15	0.0	0.0	0.0	4.2289096664581285	0.0
16	0.0	0.0	0.0	4.6105193854929025	0.0
17	0.0	0.0	0.0	5.054534618678907	0.0
18	0.0	0.0	0.0	5.501670127572472	0.0
19	0.0	0.0	0.0	5.792323809731828	0.0
20	0.0	0.0	0.0	6.08328951946194	0.0
21	0.0	0.0	0.0	6.461154907647639	0.0
22	0.0	0.0	0.0	6.861798308498539	0.0
23	0.0	0.0	0.0	7.239195655328105	0.0
24	0.0	0.0	0.0	7.548102950376696	0.0
25	0.0	0.0	0.0	7.833452163833196	0.0
26	0.0	0.0	0.0	8.094619240556096	0.0
27	0.0	0.0	0.0	8.359842675698825	0.0
28	0.0	0.0	0.0	8.626314221124579	0.0
29	0.0	0.0	0.0	8.901834566102261	0.0
30	0.0	0.0	0.0	9.217450453922108	0.0
31	0.0	0.0	0.0	9.517464963204148	0.0
32	0.0	0.0	0.0	9.812331017568713	0.0
33	0.0	0.0	0.0	10.09955239644975	0.0
34	0.0	0.0	0.0	10.411267939635145	0.0
35	0.0	0.0	0.0	10.71580684869315	0.0
36	0.0	0.0	0.0	11.034855039791315	0.0
37	0.0	0.0	0.0	11.332685356078063	0.0
38	1.56013785378076E-4	0.0	0.0	11.640968595985141	0.0
39	1.56013785378076E-4	0.0	0.0	11.96142091115171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAC	25	3.888867E-5	45.000004	24
TACGGGT	80	0.0	45.0	4
TGACGAA	20	7.03095E-4	45.0	22
GCGCAAA	20	7.03095E-4	45.0	1
CGCACTC	20	7.03095E-4	45.0	21
CAGTACG	20	7.03095E-4	45.0	1
TCAACGG	60	3.6379788E-12	41.250004	2
CACGACC	100	0.0	40.500004	27
TACGCGG	50	1.0804797E-9	40.500004	2
TGTTACG	45	1.925946E-8	40.0	1
CATAGCG	40	3.4550249E-7	39.375	1
ATGTGCG	35	6.243963E-6	38.57143	1
CGAGATA	245	0.0	38.57143	19
AACACGG	35	6.243963E-6	38.57143	2
AACACGT	105	0.0	38.571426	41
TACCGGG	65	9.094947E-12	38.07692	3
TATGGGA	485	0.0	38.041237	4
TTACACG	225	0.0	38.0	34
GCCGCAT	30	1.1393583E-4	37.500004	19
ACGGGTA	90	0.0	37.5	5
>>END_MODULE