##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936226.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243818
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757720922983538	31.0	31.0	34.0	30.0	34.0
2	31.88186680228695	33.0	31.0	34.0	30.0	34.0
3	31.846184449056263	33.0	31.0	34.0	30.0	34.0
4	35.60539008604779	37.0	35.0	37.0	33.0	37.0
5	31.12545423225521	37.0	35.0	37.0	0.0	37.0
6	33.35252524423956	37.0	35.0	37.0	17.0	37.0
7	29.670750313758624	35.0	32.0	37.0	0.0	37.0
8	32.67905979049947	35.0	35.0	37.0	17.0	37.0
9	36.42854916372048	39.0	35.0	39.0	32.0	39.0
10	36.60139940447383	38.0	35.0	39.0	32.0	39.0
11	36.74223396139744	39.0	35.0	39.0	33.0	39.0
12	36.81091223781673	39.0	35.0	39.0	33.0	39.0
13	36.6538729708225	39.0	35.0	39.0	33.0	39.0
14	37.82777727649312	40.0	37.0	41.0	33.0	41.0
15	37.915883979033545	40.0	37.0	41.0	33.0	41.0
16	37.949909358619955	40.0	37.0	41.0	33.0	41.0
17	37.92512857951423	40.0	36.0	41.0	33.0	41.0
18	37.84458489529075	40.0	36.0	41.0	33.0	41.0
19	37.75611726779811	40.0	36.0	41.0	33.0	41.0
20	37.752877146067966	39.0	36.0	41.0	33.0	41.0
21	37.64846729937904	39.0	36.0	41.0	33.0	41.0
22	37.63680286115053	39.0	36.0	41.0	33.0	41.0
23	37.58149521364297	39.0	36.0	41.0	33.0	41.0
24	37.52772970002215	39.0	36.0	41.0	33.0	41.0
25	37.40258717568022	39.0	35.0	41.0	32.0	41.0
26	37.24284507296426	39.0	35.0	41.0	32.0	41.0
27	37.19406688595592	39.0	35.0	41.0	32.0	41.0
28	37.068333757146725	39.0	35.0	41.0	32.0	41.0
29	37.026794576282306	39.0	35.0	41.0	31.0	41.0
30	36.845109877039434	39.0	35.0	40.0	31.0	41.0
31	36.739682878212435	39.0	35.0	40.0	31.0	41.0
32	36.60590686495665	39.0	35.0	40.0	30.0	41.0
33	36.43431165869624	39.0	35.0	40.0	30.0	41.0
34	36.33571352402202	39.0	35.0	40.0	30.0	41.0
35	36.252503916856014	39.0	35.0	40.0	30.0	41.0
36	36.085075753225766	39.0	35.0	40.0	29.0	41.0
37	35.97385344806372	38.0	35.0	40.0	29.0	41.0
38	35.84387534964605	38.0	35.0	40.0	28.0	41.0
39	35.83557817716493	38.0	35.0	40.0	28.0	41.0
40	35.71040284146371	38.0	35.0	40.0	27.0	41.0
41	35.631741708979646	38.0	35.0	40.0	27.0	41.0
42	35.566127193234294	38.0	34.0	40.0	27.0	41.0
43	35.35758229499053	38.0	34.0	40.0	26.0	41.0
44	35.200936764307805	38.0	34.0	40.0	26.0	41.0
45	35.06266149340902	38.0	34.0	40.0	25.0	41.0
46	34.90765242927101	38.0	34.0	40.0	24.0	41.0
47	34.7091231984513	38.0	33.0	40.0	23.0	41.0
48	34.63553962381776	38.0	33.0	40.0	23.0	41.0
49	34.412951463796766	37.0	33.0	40.0	23.0	41.0
50	34.215513210673535	37.0	33.0	40.0	22.0	41.0
51	32.21328613966155	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	12.0
10	11.0
11	9.0
12	9.0
13	7.0
14	4.0
15	16.0
16	29.0
17	67.0
18	99.0
19	186.0
20	391.0
21	544.0
22	888.0
23	1252.0
24	1742.0
25	2305.0
26	2951.0
27	3419.0
28	4076.0
29	4713.0
30	5771.0
31	7216.0
32	9134.0
33	12722.0
34	18503.0
35	21651.0
36	24509.0
37	36590.0
38	44312.0
39	40675.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78921982790442	19.211050865809742	25.272129211132892	11.727600095152942
2	30.339433511881815	24.348489447046568	25.710570999680087	19.60150604139153
3	32.587421765415186	24.154902427220303	26.34670122796512	16.910974579399387
4	28.884659869246732	26.802368980140923	25.45792353312717	18.855047617485173
5	22.527459006307986	40.08317679580671	20.70027643570204	16.68908776218327
6	29.241073259562462	35.893576356134496	22.175557177894987	12.689793206408059
7	72.58282817511422	17.742332395475312	6.130392341828741	3.544447087581721
8	83.63082299091946	4.334790704541913	7.771780590440411	4.26260571409822
9	76.92910285540854	7.62700046756187	10.167830102781583	5.276066574248005
10	35.570384467102514	33.89249358127784	17.43267519215152	13.104446759468127
11	26.62559778195211	26.303636318893602	29.23081970978353	17.83994618937076
12	27.185031457890723	24.23118883757557	29.63850084899392	18.945278855539787
13	23.93629674593344	27.90811178830111	29.982199837583774	18.173391628181676
14	19.597814763471114	27.42783551665587	29.451065959035017	23.523283760838
15	18.305867491325497	29.257478939208752	33.35520757286172	19.081445996604025
16	21.482007070847928	28.203003879943232	29.995324381300808	20.31966466790803
17	21.256839117702548	28.415867573353893	27.902369800424907	22.42492350851865
18	21.358964473500727	27.169035920235586	30.917323577422508	20.554676028841186
19	19.23483910129687	29.44286311921187	28.866203479644653	22.456094299846608
20	21.608330804124385	28.352705706715664	31.466503703582184	18.572459785577767
21	21.418845204209696	29.315719101953096	29.457218088902376	19.80821760493483
22	21.393416400757943	25.619109335652002	29.351401455183783	23.63607280840627
23	21.063252097876283	27.481564117497477	29.690178739879748	21.76500504474649
24	20.809374205349894	25.661353960741206	32.497600669351726	21.03167116455717
25	18.780811917085696	28.86251220172424	30.583467996620428	21.773207884569636
26	18.9095965023091	30.62038077582459	28.273548302422295	22.196474419444012
27	20.443937691228705	29.384212814476374	29.117210378232944	21.054639116061978
28	17.950684526983242	30.34025379586413	29.60076778580745	22.108293891345184
29	20.01164803254887	26.43447161407279	29.5339146412488	24.01996571212954
30	20.167912131179815	30.92101485534292	26.936895553240532	21.974177460236735
31	19.957099147724943	29.78492153983709	26.887268372310498	23.370710940127474
32	20.737189214906202	31.075228244018078	25.65848296680311	22.529099574272614
33	20.026003002239374	29.649164540764012	26.46851339933885	23.85631905765776
34	18.490431387346298	27.625523956393707	28.494204693664948	25.38983996259505
35	19.546547014576447	28.311281365608775	26.369669179469934	25.772502440344848
36	19.790581499315063	31.2692254058355	25.78767769401767	23.15251540083177
37	18.74307885389922	31.254050152162677	28.339581162998627	21.663289830939473
38	17.988827732160875	31.13510897472705	27.33842456258357	23.53763873052851
39	19.96202085161883	29.4108720439016	27.36713450196458	23.259972602514992
40	22.525408296352197	26.705165328236635	27.5553896759058	23.21403669950537
41	18.728723884208712	27.164524358332855	27.84248906971594	26.264262687742495
42	19.785659795421175	28.52291463304596	27.560721521790843	24.13070404974202
43	20.33812105751011	29.725861093110435	27.535702860330247	22.400314989049207
44	19.46164762240688	29.712326407402244	25.92015355716149	24.90587241302939
45	20.429992863529353	28.261654184678736	25.278691482991412	26.029661468800498
46	20.19908292250777	28.283391710210076	26.83230934549541	24.685216021786744
47	18.4711547137619	27.970863512948185	30.28611505303136	23.27186672025855
48	19.354190420723654	27.036970199082923	29.75457103249145	23.854268347701975
49	20.336070347554323	27.731340590112296	28.725114634686527	23.20747442764685
50	18.809111714475552	25.606805075917283	30.04987326612473	25.534209943482434
51	18.761125101510142	25.51370284392457	27.718216046395263	28.006956008170032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	234.0
1	640.5
2	1047.0
3	1037.5
4	1028.0
5	823.5
6	619.0
7	615.0
8	611.0
9	671.5
10	732.0
11	731.5
12	731.0
13	701.0
14	671.0
15	634.5
16	598.0
17	659.5
18	721.0
19	899.5
20	1078.0
21	1078.0
22	1078.0
23	1212.0
24	1346.0
25	1523.0
26	2064.5
27	2429.0
28	3202.5
29	3976.0
30	4845.5
31	5715.0
32	6555.0
33	7395.0
34	8159.5
35	8924.0
36	8844.5
37	8765.0
38	9842.5
39	10920.0
40	11984.5
41	13049.0
42	14163.0
43	15277.0
44	17234.0
45	19191.0
46	21053.0
47	22915.0
48	23827.5
49	24740.0
50	24001.5
51	23263.0
52	19652.0
53	16041.0
54	14048.0
55	12055.0
56	10694.0
57	9333.0
58	8291.5
59	7250.0
60	6511.0
61	5772.0
62	5105.5
63	4439.0
64	3769.0
65	3099.0
66	2565.0
67	2031.0
68	1743.5
69	1456.0
70	1342.5
71	1229.0
72	1107.5
73	986.0
74	814.0
75	472.5
76	303.0
77	235.0
78	167.0
79	138.0
80	109.0
81	81.5
82	54.0
83	49.0
84	44.0
85	35.5
86	27.0
87	21.0
88	15.0
89	12.0
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	243818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.01714393523038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9982063975238	30.01214020293826
2	9.72030050528344	7.7795732882724
3	4.014594799577735	4.819578538089886
4	2.3081101579395096	3.694559056345307
5	1.505601164304236	3.012492925050653
6	1.0249157006836187	2.460851946944032
7	0.8148079820434769	2.2824401807905894
8	0.5985507691992333	1.9161833826870862
9	0.4653117281103629	1.6758401758688857
>10	4.412262091442979	37.493540263639275
>50	0.11069089567383084	2.690941603983299
>100	0.02562289251709047	1.8936255731734324
>500	0.001024915700683619	0.2682328622168995
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	654	0.2682328622168995	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	412	0.16897850035682355	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	398	0.1632365124806208	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	353	0.14478012287854053	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	331	0.1357569990730791	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	262	0.10745720168322272	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23090994102158166	0.0
2	0.0	0.0	0.0	0.8863168428910089	0.0
3	0.0	0.0	0.0	1.1730060947099885	0.0
4	0.0	0.0	0.0	1.5150645153352091	0.0
5	0.0	0.0	0.0	2.5354977893346677	0.0
6	0.0	0.0	0.0	3.1158487068223018	0.0
7	0.0	0.0	0.0	3.7474673732046035	0.0
8	0.0	0.0	0.0	4.960667383048011	0.0
9	0.0	0.0	0.0	5.326514039160357	0.0
10	0.0	0.0	0.0	6.407238185859945	0.0
11	0.0	0.0	0.0	7.952653208540797	0.0
12	0.0	0.0	0.0	8.960372080814379	0.0
13	0.0	0.0	0.0	9.398403727370416	0.0
14	0.0	0.0	0.0	9.557128677948306	0.0
15	0.0	0.0	0.0	9.975883650919949	0.0
16	0.0	0.0	0.0	10.813393596863234	0.0
17	0.0	0.0	0.0	11.92364796692615	0.0
18	0.0	0.0	0.0	12.938339253049406	0.0
19	0.0	0.0	0.0	13.527713294342501	0.0
20	0.0	0.0	0.0	14.135543725237678	0.0
21	0.0	0.0	0.0	14.95500742357004	0.0
22	0.0	0.0	0.0	15.849527106284196	0.0
23	0.0	0.0	0.0	16.730101961299003	0.0
24	0.0	0.0	0.0	17.40560582073514	0.0
25	0.0	0.0	0.0	18.029021647294293	0.0
26	0.0	0.0	0.0	18.56876850765735	0.0
27	0.0	0.0	0.0	19.137225307401422	0.0
28	0.0	0.0	0.0	19.682304013649524	0.0
29	0.0	0.0	0.0	20.262244789146003	0.0
30	0.0	0.0	0.0	20.961536884069265	0.0
31	0.0	0.0	0.0	21.56608617903518	0.0
32	0.0	0.0	0.0	22.172686183956888	0.0
33	0.0	0.0	0.0	22.743193693656742	0.0
34	0.0	0.0	0.0	23.268585584329294	0.0
35	0.0	0.0	0.0	23.85180749575503	0.0
36	0.0	0.0	0.0	24.44692352492433	0.0
37	0.0	0.0	0.0	25.053113387854875	0.0
38	0.0	0.0	0.0	25.635515015298296	0.0
39	0.0	0.0	0.0	26.221607920662134	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATGG	35	1.206663E-7	45.000004	2
GAGTCTC	35	1.206663E-7	45.000004	27
GTAATCC	35	1.206663E-7	45.000004	9
CACCTAT	35	1.206663E-7	45.000004	15
CGTGTCC	35	1.206663E-7	45.000004	33
CGCACGG	35	1.206663E-7	45.000004	2
TGTCCGT	35	1.206663E-7	45.000004	35
AACCGTC	20	7.022344E-4	45.0	25
ACGCCTA	20	7.022344E-4	45.0	12
TTAATAG	20	7.022344E-4	45.0	1
GCGAATC	25	3.881737E-5	45.0	1
TAGACGG	25	3.881737E-5	45.0	2
TCCGAGG	20	7.022344E-4	45.0	2
CGCATGG	50	2.1827873E-11	45.0	2
CGGCAGT	40	6.7775545E-9	45.0	12
TAGCGAG	20	7.022344E-4	45.0	1
GCTATCA	25	3.881737E-5	45.0	9
TTCGTGG	20	7.022344E-4	45.0	16
CGTCTGT	20	7.022344E-4	45.0	19
TTGTACC	20	7.022344E-4	45.0	22
>>END_MODULE