##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537004
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.949097213428576	31.0	31.0	34.0	30.0	34.0
2	32.08978145414187	33.0	31.0	34.0	30.0	34.0
3	32.05098658482991	33.0	31.0	34.0	30.0	34.0
4	35.76164237137898	37.0	35.0	37.0	35.0	37.0
5	31.164968976022525	37.0	35.0	37.0	0.0	37.0
6	33.400609678885075	37.0	35.0	37.0	17.0	37.0
7	29.517936551683043	35.0	32.0	37.0	0.0	37.0
8	32.59124699257361	35.0	33.0	37.0	17.0	37.0
9	36.47793684963241	39.0	35.0	39.0	32.0	39.0
10	36.773850846548626	38.0	35.0	39.0	33.0	39.0
11	36.872343595206	39.0	37.0	39.0	33.0	39.0
12	36.87977743182546	39.0	35.0	39.0	33.0	39.0
13	36.71407661767883	39.0	35.0	39.0	33.0	39.0
14	37.960203275953255	40.0	37.0	41.0	33.0	41.0
15	38.06524532405717	40.0	37.0	41.0	33.0	41.0
16	38.09447788098413	40.0	37.0	41.0	33.0	41.0
17	38.075126069824435	40.0	37.0	41.0	33.0	41.0
18	38.01064610319476	40.0	37.0	41.0	33.0	41.0
19	37.94203022696293	40.0	37.0	41.0	33.0	41.0
20	37.90321487363222	40.0	36.0	41.0	33.0	41.0
21	37.81165130985989	40.0	36.0	41.0	33.0	41.0
22	37.771398350850276	40.0	36.0	41.0	33.0	41.0
23	37.702490111805496	40.0	36.0	41.0	33.0	41.0
24	37.65789081645574	40.0	36.0	41.0	33.0	41.0
25	37.52058643883472	39.0	36.0	41.0	33.0	41.0
26	37.34602163112379	39.0	35.0	41.0	32.0	41.0
27	37.298353457329924	39.0	35.0	41.0	32.0	41.0
28	37.20287372161101	39.0	35.0	41.0	32.0	41.0
29	37.201132580018026	39.0	35.0	41.0	32.0	41.0
30	37.03799971694811	39.0	35.0	41.0	31.0	41.0
31	36.922380466439726	39.0	35.0	41.0	31.0	41.0
32	36.861367885527855	39.0	35.0	41.0	31.0	41.0
33	36.7101306507959	39.0	35.0	41.0	30.0	41.0
34	36.60063239752404	39.0	35.0	41.0	30.0	41.0
35	36.52480242232833	39.0	35.0	41.0	30.0	41.0
36	36.407239052223076	39.0	35.0	40.0	30.0	41.0
37	36.295759435683905	39.0	35.0	40.0	30.0	41.0
38	36.194220154784695	39.0	35.0	40.0	30.0	41.0
39	36.16676412093765	39.0	35.0	40.0	30.0	41.0
40	36.0047001512093	39.0	35.0	40.0	29.0	41.0
41	35.91595034673857	38.0	35.0	40.0	29.0	41.0
42	35.87546461478872	38.0	35.0	40.0	28.0	41.0
43	35.75404093824255	38.0	35.0	40.0	28.0	41.0
44	35.602976886578126	38.0	34.0	40.0	28.0	41.0
45	35.46411758571631	38.0	34.0	40.0	27.0	41.0
46	35.319729461977936	38.0	34.0	40.0	26.0	41.0
47	35.178471668739896	38.0	34.0	40.0	26.0	41.0
48	35.09598252526983	38.0	34.0	40.0	26.0	41.0
49	34.961827099984355	38.0	34.0	40.0	26.0	41.0
50	34.75785469009542	37.0	34.0	40.0	24.0	41.0
51	32.73708389509203	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	17.0
10	27.0
11	12.0
12	9.0
13	12.0
14	17.0
15	32.0
16	49.0
17	126.0
18	269.0
19	460.0
20	767.0
21	1192.0
22	1740.0
23	2304.0
24	3179.0
25	4200.0
26	5231.0
27	6345.0
28	7514.0
29	8994.0
30	11525.0
31	14775.0
32	19397.0
33	27745.0
34	40132.0
35	47315.0
36	53828.0
37	83029.0
38	101597.0
39	95144.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83150963493755	20.21232616516823	24.101496450678205	10.854667749216022
2	32.869028908537	24.396280102196634	24.600189197845825	18.134501791420547
3	33.462693015322046	24.21248258858407	25.03854719890355	17.286277197190337
4	29.3441017199127	26.917862809215578	24.53538521128334	19.20265025958838
5	22.39517768955166	40.15519437471602	20.38308839412742	17.066539541604904
6	27.938339379222498	36.85763979411699	22.56612613686304	12.637894689797468
7	72.96053660680367	18.950138174017326	4.817468771182338	3.2718564479966625
8	87.27998301688628	4.057697894242873	5.618021467251641	3.0442976216192057
9	81.12490782191567	6.712799159782795	7.81837751674103	4.34391550156051
10	42.60266217756292	28.648017519422574	14.728568129846334	14.02075217316817
11	30.918577887687988	26.632948730363275	24.36890600442455	18.07956737752419
12	30.16569708977959	23.656620807293802	26.989743093161316	19.18793900976529
13	24.62030077988246	26.02420093705075	28.415989452592534	20.93950883047426
14	20.723123105228268	27.74578960305696	28.220832619496317	23.310254672218456
15	21.030569604695682	27.428287312571232	30.333852261808104	21.207290820924985
16	23.571891457046874	26.354924730542045	29.717283297703556	20.355900514707525
17	23.763323923099268	25.57709067344005	27.420466141779205	23.239119261681477
18	24.074494789610508	25.421970786064907	28.066457605529937	22.437076818794647
19	23.644516614401383	27.976514141421667	26.292914019262426	22.086055224914524
20	26.232951709856913	26.683041467102665	27.00072997594059	20.083276847099835
21	25.984350209681867	27.305569418477333	26.53015619995382	20.179924171886988
22	25.027560316124276	25.844314008834196	25.94077511526916	23.187350559772366
23	24.074308571258314	27.251752314694116	27.280616159283728	21.39332295476384
24	23.01696076751756	25.8297889773633	28.829394194456654	22.32385606066249
25	22.348064446447328	26.906131053027536	26.9152557522849	23.830548748240236
26	21.968365226329787	28.11357084863427	27.205756381702926	22.71230754333301
27	21.524606893058525	28.055656941102857	27.858265487780354	22.561470678058264
28	20.271916037869364	28.24708940715525	28.56179842235812	22.919196132617262
29	21.635406812612196	26.12438641052953	28.13405486737529	24.106151909482985
30	23.178225860515006	27.36832500316571	27.174657916887025	22.278791219432257
31	22.6854921006175	27.50202978003888	27.001474849349353	22.811003269994266
32	23.755875189011626	26.52233502916179	26.83350589567303	22.88828388615355
33	23.031485798988463	26.58434574044141	26.817491117384602	23.56667734318553
34	21.17619235610908	26.74616948849543	27.713201391423524	24.364436763971963
35	21.39183320794631	26.15809193227611	27.657335885766216	24.792738974011368
36	23.214166002487875	27.756031612427467	26.844678996804493	22.18512338828016
37	21.706356004796984	27.46068930585247	28.607608136997115	22.22534655235343
38	21.5646438387796	27.425307818936172	27.618416250158283	23.391632092125946
39	22.737819457583182	27.81003493456287	26.23891069712702	23.21323491072692
40	23.270776381553954	26.187514431922292	28.08284482052275	22.458864366000995
41	20.20320146591087	26.097570967814022	28.670922376742077	25.02830518953304
42	20.76837416481069	25.458469583094352	27.945229458253568	25.82792679384139
43	21.77451192169891	25.797573202434247	28.388615354820445	24.0392995210464
44	21.521254962719087	26.69253860306441	26.952126986018726	24.83407944819778
45	21.74453076699615	26.368146233547606	26.86367326872798	25.023649730728266
46	21.95849565366366	27.462923926078766	26.559951136304385	24.018629283953192
47	20.715674371140626	26.131090271208407	29.747450670758507	23.40578468689246
48	20.69500413404742	24.808567533947606	29.98189957616703	24.514528755837944
49	21.380660106814847	25.862563407348922	28.77576330902563	23.9810131768106
50	20.825170762228957	25.345248824962198	29.769983091373618	24.059597321435223
51	20.703011523191634	25.830906287476445	27.85621708590625	25.609865103425673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	586.0
2	990.0
3	1252.5
4	1515.0
5	1183.5
6	852.0
7	753.0
8	654.0
9	674.5
10	695.0
11	740.0
12	785.0
13	862.5
14	940.0
15	938.5
16	937.0
17	963.0
18	989.0
19	1098.5
20	1208.0
21	1435.0
22	1662.0
23	1758.5
24	1855.0
25	2483.0
26	4135.5
27	5160.0
28	6355.5
29	7551.0
30	8815.5
31	10080.0
32	11453.5
33	12827.0
34	14318.0
35	15809.0
36	17712.0
37	19615.0
38	21138.0
39	22661.0
40	25308.0
41	27955.0
42	30228.5
43	32502.0
44	35512.5
45	38523.0
46	41128.5
47	43734.0
48	44280.5
49	44827.0
50	43028.0
51	41229.0
52	38344.5
53	35460.0
54	32800.5
55	30141.0
56	28541.0
57	26941.0
58	25522.0
59	24103.0
60	22752.5
61	21402.0
62	20085.5
63	18769.0
64	15777.0
65	12785.0
66	11158.5
67	9532.0
68	8074.5
69	6617.0
70	5558.0
71	4499.0
72	3710.0
73	2921.0
74	2419.0
75	1612.5
76	1308.0
77	1075.0
78	842.0
79	617.5
80	393.0
81	297.0
82	201.0
83	183.5
84	166.0
85	133.0
86	100.0
87	65.5
88	31.0
89	21.5
90	12.0
91	10.5
92	9.0
93	7.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	537004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.050116478449375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.83892029369696	25.537513277953668
2	8.842295345783528	6.37531554304694
3	4.524812049095124	4.893600042389113
4	2.970102934046698	4.282902269014708
5	2.1611290193266646	3.895447643584167
6	1.5467116799423273	3.3455481732299406
7	1.110992513697947	2.803598666784738
8	0.86939446584245	2.507341740747168
9	0.6849657093286635	2.222378424453782
>10	6.389706052703574	42.002473068005784
>50	0.043037602260787546	0.9866868438965744
>100	0.01741998186740257	1.042282639478267
>500	5.123524078647815E-4	0.10491166741514366
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	568	0.10577202404451364	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08491556859911659	0.0
2	0.0	0.0	0.0	0.32737186315185735	0.0
3	0.0	0.0	0.0	0.4614490767294098	0.0
4	0.0	0.0	0.0	0.6284869386447773	0.0
5	0.0	0.0	0.0	1.1564159671063903	0.0
6	0.0	0.0	0.0	1.5128378932000506	0.0
7	0.0	0.0	0.0	1.8310850570945467	0.0
8	0.0	0.0	0.0	2.3992372496294254	0.0
9	0.0	0.0	0.0	2.586572911933617	0.0
10	0.0	0.0	0.0	3.099604472219946	0.0
11	0.0	0.0	0.0	3.8355394000789564	0.0
12	0.0	0.0	0.0	4.36365464689276	0.0
13	0.0	0.0	0.0	4.598848425710051	0.0
14	1.8621835219104514E-4	0.0	0.0	4.693447348623102	0.0
15	1.8621835219104514E-4	0.0	0.0	4.89270098546752	0.0
16	1.8621835219104514E-4	0.0	0.0	5.3305003314686665	0.0
17	1.8621835219104514E-4	0.0	0.0	5.843718110107187	0.0
18	1.8621835219104514E-4	0.0	0.0	6.3668054614118335	0.0
19	1.8621835219104514E-4	0.0	0.0	6.702184713707905	0.0
20	1.8621835219104514E-4	0.0	0.0	7.02359758958965	0.0
21	1.8621835219104514E-4	0.0	0.0	7.4528308913900085	0.0
22	1.8621835219104514E-4	0.0	0.0	7.915024841528182	0.0
23	1.8621835219104514E-4	0.0	0.0	8.401613395803384	0.0
24	1.8621835219104514E-4	0.0	0.0	8.762318343997437	0.0
25	1.8621835219104514E-4	0.0	0.0	9.112595064468794	0.0
26	1.8621835219104514E-4	0.0	0.0	9.416130978540197	0.0
27	1.8621835219104514E-4	0.0	0.0	9.709611101593284	0.0
28	1.8621835219104514E-4	0.0	0.0	10.028044483839972	0.0
29	1.8621835219104514E-4	0.0	0.0	10.360630460853178	0.0
30	1.8621835219104514E-4	0.0	0.0	10.720590535638468	0.0
31	1.8621835219104514E-4	0.0	0.0	11.065839360600666	0.0
32	1.8621835219104514E-4	0.0	0.0	11.40512919829275	0.0
33	1.8621835219104514E-4	0.0	0.0	11.755964573820679	0.0
34	1.8621835219104514E-4	0.0	0.0	12.092088699525515	0.0
35	1.8621835219104514E-4	0.0	0.0	12.460801036863785	0.0
36	1.8621835219104514E-4	0.0	0.0	12.809960447221995	0.0
37	1.8621835219104514E-4	0.0	0.0	13.146084572926831	0.0
38	1.8621835219104514E-4	0.0	0.0	13.498595913624479	0.0
39	1.8621835219104514E-4	0.0	0.0	13.853714311252803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.029925E-4	45.000004	1
ATAGGCG	30	2.1633332E-6	45.000004	5
GCGAATG	40	6.8030204E-9	45.000004	1
CGCATGG	40	6.8030204E-9	45.000004	2
TTATGCG	20	7.029925E-4	45.000004	1
TAAACGG	30	2.1633332E-6	45.000004	2
CGCGCAA	20	7.029925E-4	45.000004	18
CCAGCGT	20	7.029925E-4	45.000004	28
TACGGGT	20	7.029925E-4	45.000004	4
AACGACG	20	7.029925E-4	45.000004	13
TTACGAG	20	7.029925E-4	45.000004	1
CGATCCA	20	7.029925E-4	45.000004	24
CGATAGT	20	7.029925E-4	45.000004	10
GTACGAG	25	3.8880207E-5	45.0	1
CACGTGA	135	0.0	43.333332	43
ACACGAC	135	0.0	41.666664	26
ATTACGG	60	3.6379788E-12	41.250004	2
TACGGGA	190	0.0	40.263157	4
GCGAGAC	140	0.0	40.17857	21
GACACGA	140	0.0	40.17857	25
>>END_MODULE