FastQCFastQC Report
Sat 14 Jan 2017
SRR2936222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656520
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.4738621824163773No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11140.1696825686955462No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG11000.16755011271553036No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC10760.16389447389264605No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC9020.1373910924267349No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.12916590507524522No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC6680.101748613903613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAA253.888971E-545.0000049
ACACGAC406.8066583E-945.026
CGAAACG207.0310745E-445.02
TAACGCC351.2108649E-745.012
TATAGCG207.0310745E-445.01
TATCGCG406.8066583E-945.01
CGTTATT2950.041.186441
GCTACGA1150.041.08695610
CGTTTTT11300.041.0176961
ACTACGG451.9261279E-840.02
TTAACGG750.039.02
TACGGGA2800.038.5714264
TATAACG356.2441977E-638.5714261
CGTTTTA2350.038.297871
CGAATAT1250.037.814
GTATTAC301.1393884E-437.50000418
TACGACG301.1393884E-437.5000041
GCGATAC301.1393884E-437.5000049
TTACGAA301.1393884E-437.50000423
TCGACGA301.1393884E-437.50000445
Produced by FastQC (version 0.11.5)