##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936222.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656520
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94003990739048	31.0	31.0	34.0	30.0	34.0
2	32.07489794674953	33.0	31.0	34.0	30.0	34.0
3	31.905536769633827	33.0	31.0	34.0	30.0	34.0
4	35.68665234874794	37.0	35.0	37.0	33.0	37.0
5	31.199593310180955	37.0	35.0	37.0	0.0	37.0
6	33.412448973374765	37.0	35.0	37.0	17.0	37.0
7	29.547773106683728	35.0	32.0	37.0	0.0	37.0
8	32.60971638335466	35.0	33.0	37.0	17.0	37.0
9	36.54481356242003	39.0	35.0	39.0	32.0	39.0
10	36.87538231889356	38.0	37.0	39.0	33.0	39.0
11	36.91285566319381	39.0	37.0	39.0	33.0	39.0
12	36.86864223481387	39.0	35.0	39.0	33.0	39.0
13	36.65567233290684	39.0	35.0	39.0	33.0	39.0
14	37.820506610613535	40.0	37.0	41.0	33.0	41.0
15	37.950252848351916	40.0	37.0	41.0	33.0	41.0
16	38.06064247852312	40.0	37.0	41.0	33.0	41.0
17	38.042440443550845	40.0	37.0	41.0	33.0	41.0
18	37.961503076829345	40.0	37.0	41.0	33.0	41.0
19	37.85611405593127	40.0	36.0	41.0	33.0	41.0
20	37.75942850179736	40.0	36.0	41.0	33.0	41.0
21	37.67277158350088	40.0	36.0	41.0	33.0	41.0
22	37.65829677694511	39.0	36.0	41.0	33.0	41.0
23	37.61113446658137	39.0	35.0	41.0	33.0	41.0
24	37.547901054042526	39.0	35.0	41.0	33.0	41.0
25	37.37011515262292	39.0	35.0	41.0	32.0	41.0
26	37.211452811795525	39.0	35.0	41.0	32.0	41.0
27	37.16815786266984	39.0	35.0	41.0	32.0	41.0
28	37.0406446109791	39.0	35.0	41.0	32.0	41.0
29	36.95334643270578	39.0	35.0	41.0	31.0	41.0
30	36.788909705721075	39.0	35.0	41.0	31.0	41.0
31	36.66415036861025	39.0	35.0	41.0	31.0	41.0
32	36.49877993054286	39.0	35.0	40.0	30.0	41.0
33	36.28970023761652	39.0	35.0	40.0	30.0	41.0
34	36.143761043075614	39.0	35.0	40.0	30.0	41.0
35	35.95949704502529	39.0	35.0	40.0	29.0	41.0
36	35.76767958325718	38.0	35.0	40.0	28.0	41.0
37	35.63995917869981	38.0	35.0	40.0	27.0	41.0
38	35.4343660513008	38.0	34.0	40.0	26.0	41.0
39	35.334062937915064	38.0	34.0	40.0	25.0	41.0
40	35.182705782002074	38.0	34.0	40.0	25.0	41.0
41	35.00350484372144	38.0	34.0	40.0	24.0	41.0
42	34.93710321086943	38.0	34.0	40.0	24.0	41.0
43	34.8083607506245	38.0	34.0	40.0	23.0	41.0
44	34.511970693962105	38.0	33.0	40.0	23.0	41.0
45	34.409842807530616	38.0	33.0	40.0	23.0	41.0
46	34.32925424968013	37.0	33.0	40.0	23.0	41.0
47	34.1391275208676	37.0	33.0	40.0	22.0	41.0
48	33.99289130567233	37.0	33.0	40.0	21.0	41.0
49	33.88545512703345	37.0	33.0	40.0	20.0	41.0
50	33.71620209589959	37.0	33.0	40.0	20.0	41.0
51	31.80439590568452	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	24.0
10	24.0
11	17.0
12	12.0
13	22.0
14	22.0
15	53.0
16	88.0
17	208.0
18	387.0
19	681.0
20	1091.0
21	1707.0
22	2743.0
23	4040.0
24	5731.0
25	7877.0
26	9524.0
27	10787.0
28	11749.0
29	13070.0
30	15583.0
31	19307.0
32	24875.0
33	34645.0
34	48509.0
35	56459.0
36	64520.0
37	97965.0
38	117320.0
39	107456.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95725948942911	19.764211295923964	25.099159203070737	12.17937001157619
2	34.37686589898251	24.338481691342228	25.165874611588375	16.118777798086885
3	33.04210077377688	24.415097788338514	26.38807652470603	16.15472491317858
4	28.34704197891915	25.39739840370438	27.491470176079936	18.764089441296534
5	22.242886736123804	39.1326996892707	22.611801620666544	16.01261195393895
6	27.19323097544629	34.99543045147139	24.962225065496863	12.84911350758545
7	71.81060744531773	18.519161640163283	6.93307134588436	2.737159568634619
8	87.08219094620118	4.079235971486018	6.6873819533296786	2.151191128983123
9	81.6374215560836	5.544080911472613	8.310028635837446	4.508468896606348
10	45.44659720952903	27.52269542435874	15.082251873514895	11.948455492597333
11	31.86681289221958	25.71604825443246	25.442332297569	16.974806555778958
12	29.460793273624564	21.995369524157677	29.6422043502102	18.901632852007555
13	25.25787485529763	23.187564735270822	32.142356668494486	19.412203740937063
14	21.701090598915492	25.072808139889112	31.66590507524523	21.56019618595016
15	20.51331261804667	25.740571498202648	33.404313653811	20.34180222993968
16	25.92624748674831	24.518826539937855	29.489886065923354	20.065039907390485
17	25.59023335161153	23.80277828550539	28.69219521111314	21.91479315176994
18	24.83762870895022	23.842076402851397	30.478279412660697	20.842015475537686
19	24.866873819533296	26.051453116432093	27.34874794370316	21.732925120331444
20	25.238835069761773	27.337019435813076	28.36532017303357	19.058825321391577
21	26.763693413757387	25.678882593066472	29.045116675805765	18.51230731737038
22	25.296106744653628	22.821696216413816	30.15688783281545	21.725309206117103
23	24.40215073417413	24.980503259611282	29.797873636751355	20.81947236946323
24	24.692774020593433	24.515475537683542	29.589502223846953	21.202248217876075
25	23.91762627185767	25.976207883994395	28.712453542923296	21.39371230122464
26	22.830835313471027	27.07107171144824	27.605556571010787	22.492536404069945
27	22.812100164503747	26.21778468287333	29.22439529641138	21.74571985621154
28	21.751203314445867	26.485712544933893	29.872204959483334	21.890879181136906
29	23.474380064582952	26.323798208736974	28.193809784926582	22.008011941753487
30	23.834612806921342	26.42828855175775	28.39288978248949	21.344208858831415
31	25.295192834947905	25.132059952476695	27.843782367635413	21.728964844939984
32	25.526564308779626	25.831200877353318	27.24776092122098	21.394473892646072
33	24.802138548711387	25.747882775848414	27.80417961372083	21.645799061719366
34	23.102418814354476	25.084079692926338	29.07756047035886	22.735941022360322
35	23.030676902455372	26.22905623591056	28.22305489550966	22.517211966124414
36	25.763723877414247	26.483427770669593	26.35121549990861	21.401632852007555
37	23.278498750990067	26.584110156583197	27.957564125997685	22.17982696642905
38	23.679552793517335	26.50140132821544	28.006915250106623	21.812130628160602
39	23.657771278864313	26.476725766160968	26.94860781088162	22.916895144093097
40	25.275848412843477	25.124444038262357	27.68491439712423	21.91479315176994
41	22.28660208371413	25.177603119478466	27.806464387985137	24.729330408822275
42	22.26284043136538	25.539663681228298	28.06068360445988	24.136812282946448
43	22.351185036251753	25.685432279290804	28.153293121306284	23.81008956315116
44	23.39822092243953	25.930512398708338	28.023060988241028	22.6482056906111
45	22.339304210077376	25.30707366112228	27.37646987144337	24.977152257356973
46	23.596843965149578	26.169347468470118	26.6593553890209	23.574453177359413
47	22.183482605251932	25.50082251873515	29.387375860598308	22.928319015414612
48	22.396423566684945	26.380917565344543	28.572929994516542	22.64972887345397
49	23.02397489794675	25.68954487296655	28.49265825869737	22.793821970389324
50	22.385913605069153	25.953360141351368	28.567903491135077	23.092822762444403
51	22.21074757813928	25.817339913483217	27.100316822031317	24.87159568634619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	638.0
2	980.0
3	3633.5
4	6287.0
5	4653.5
6	3020.0
7	2408.5
8	1797.0
9	1763.0
10	1729.0
11	1708.5
12	1688.0
13	1672.5
14	1657.0
15	1599.0
16	1541.0
17	1603.5
18	1666.0
19	1747.0
20	1828.0
21	2003.5
22	2179.0
23	2459.5
24	2740.0
25	3420.5
26	5216.0
27	6331.0
28	7793.5
29	9256.0
30	10941.5
31	12627.0
32	14014.5
33	15402.0
34	17944.0
35	20486.0
36	21375.5
37	22265.0
38	23686.5
39	25108.0
40	27560.0
41	30012.0
42	32624.0
43	35236.0
44	37695.0
45	40154.0
46	43828.0
47	47502.0
48	47618.5
49	47735.0
50	46647.0
51	45559.0
52	43428.5
53	41298.0
54	39883.5
55	38469.0
56	37340.5
57	36212.0
58	35509.0
59	34806.0
60	32330.5
61	29855.0
62	27182.5
63	24510.0
64	22122.5
65	19735.0
66	17157.5
67	14580.0
68	12417.5
69	10255.0
70	8597.5
71	6940.0
72	5781.0
73	4622.0
74	3658.0
75	2170.5
76	1647.0
77	1233.5
78	820.0
79	615.5
80	411.0
81	316.0
82	221.0
83	164.0
84	107.0
85	89.0
86	71.0
87	55.5
88	40.0
89	28.5
90	17.0
91	13.5
92	10.0
93	9.5
94	9.0
95	7.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	656520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.705557490632295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58390172262663	26.27354233342282
2	7.872508857877536	5.621846352409167
3	3.8929498383413144	4.169998327831305
4	2.486511977257126	3.5512918542040035
5	1.8134014682518165	3.2374255189131125
6	1.3549926700430528	2.902846120776455
7	1.0355717356977336	2.5882966301240513
8	0.8070661413099735	2.305339720582882
9	0.6254383248998534	2.0098461659900826
>10	6.462617029442651	43.805408839972
>50	0.045129958466189585	1.0388728222285455
>100	0.016370671201943746	0.9995359404117219
>500	0.0017698022921020264	0.48452295388695293
>1k	0.0017698022921020264	1.011226419246947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3111	0.4738621824163773	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1114	0.1696825686955462	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	1100	0.16755011271553036	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	1076	0.16389447389264605	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	902	0.1373910924267349	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	848	0.12916590507524522	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	668	0.101748613903613	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04066898190458783	0.0
2	0.0	0.0	0.0	0.17486139036129897	0.0
3	0.0	0.0	0.0	0.24904039480899287	0.0
4	0.0	0.0	0.0	0.3658685188570036	0.0
5	1.523182842868458E-4	0.0	0.0	0.8689758118564552	0.0
6	1.523182842868458E-4	0.0	0.0	1.164625601657223	0.0
7	1.523182842868458E-4	0.0	0.0	1.4455005178821665	0.0
8	1.523182842868458E-4	0.0	0.0	1.8365015536464997	0.0
9	1.523182842868458E-4	0.0	0.0	2.0197404496435754	0.0
10	1.523182842868458E-4	0.0	0.0	2.702278681532931	0.0
11	3.046365685736916E-4	0.0	0.0	3.183604459879364	0.0
12	3.046365685736916E-4	0.0	0.0	3.8163346128069215	0.0
13	3.046365685736916E-4	0.0	0.0	3.9784012672881253	0.0
14	3.046365685736916E-4	0.0	0.0	4.100865167854749	0.0
15	3.046365685736916E-4	0.0	0.0	4.25150795101444	0.0
16	3.046365685736916E-4	0.0	0.0	4.500700664107719	0.0
17	3.046365685736916E-4	0.0	0.0	4.807469688661427	0.0
18	3.046365685736916E-4	0.0	0.0	5.151709011149698	0.0
19	3.046365685736916E-4	0.0	0.0	5.360537378906964	0.0
20	3.046365685736916E-4	0.0	0.0	5.5874916224943645	0.0
21	3.046365685736916E-4	0.0	0.0	5.852982392006337	0.0
22	3.046365685736916E-4	0.0	0.0	6.126089075732651	0.0
23	3.046365685736916E-4	0.0	0.0	6.417016998720526	0.0
24	3.046365685736916E-4	0.0	0.0	6.634375190397855	0.0
25	3.046365685736916E-4	0.0	0.0	6.874428806433924	0.0
26	3.046365685736916E-4	0.0	0.0	7.081581673064035	0.0
27	3.046365685736916E-4	0.0	0.0	7.337781027234509	0.0
28	3.046365685736916E-4	0.0	0.0	7.573112776457686	0.0
29	3.046365685736916E-4	0.0	0.0	7.824894900383842	0.0
30	3.046365685736916E-4	0.0	0.0	8.267988789374277	0.0
31	3.046365685736916E-4	0.0	0.0	8.54688356790349	0.0
32	3.046365685736916E-4	0.0	0.0	8.792572960458173	0.0
33	3.046365685736916E-4	0.0	0.0	9.054712727715835	0.0
34	3.046365685736916E-4	0.0	0.0	9.344117467860842	0.0
35	3.046365685736916E-4	0.0	0.0	9.665966002558948	0.0
36	3.046365685736916E-4	0.0	0.0	9.941662097118138	0.0
37	3.046365685736916E-4	0.0	0.0	10.212636324864437	0.0
38	3.046365685736916E-4	0.0	0.0	10.511180162066655	0.0
39	3.046365685736916E-4	0.0	0.0	10.995552306098825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAA	25	3.888971E-5	45.000004	9
ACACGAC	40	6.8066583E-9	45.0	26
CGAAACG	20	7.0310745E-4	45.0	2
TAACGCC	35	1.2108649E-7	45.0	12
TATAGCG	20	7.0310745E-4	45.0	1
TATCGCG	40	6.8066583E-9	45.0	1
CGTTATT	295	0.0	41.18644	1
GCTACGA	115	0.0	41.086956	10
CGTTTTT	1130	0.0	41.017696	1
ACTACGG	45	1.9261279E-8	40.0	2
TTAACGG	75	0.0	39.0	2
TACGGGA	280	0.0	38.571426	4
TATAACG	35	6.2441977E-6	38.571426	1
CGTTTTA	235	0.0	38.29787	1
CGAATAT	125	0.0	37.8	14
GTATTAC	30	1.1393884E-4	37.500004	18
TACGACG	30	1.1393884E-4	37.500004	1
GCGATAC	30	1.1393884E-4	37.500004	9
TTACGAA	30	1.1393884E-4	37.500004	23
TCGACGA	30	1.1393884E-4	37.500004	45
>>END_MODULE