FastQCFastQC Report
Sat 14 Jan 2017
SRR2936221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478000
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.29874476987447696No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA5200.10878661087866108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG302.1628312E-645.00000411
CTTCGCA253.8873746E-545.024
TCGTTCA253.8873746E-545.016
ATCCGAC253.8873746E-545.038
GTATGCG207.0291467E-445.01
CGAATAT551.8189894E-1245.014
GCGATAT406.8012014E-945.09
GTATACG207.0291467E-445.01
TATACGG351.2099372E-745.02
TTCGCAT253.8873746E-545.025
TAATCGA207.0291467E-445.013
ATGGGTA750.042.0000045
GCTACGA603.6379788E-1241.25000410
CGGGTAT1200.039.3750046
ATAACGG403.4525692E-739.3752
ACCCACC2700.039.16666413
ATAGCGG700.038.5714262
ACGGGTA700.038.5714265
ATATCAC2800.038.57142630
TTAACGG356.240636E-638.5714262
Produced by FastQC (version 0.11.5)