##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478000
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.025832635983264	33.0	31.0	34.0	30.0	34.0
2	32.16769037656904	33.0	31.0	34.0	30.0	34.0
3	32.07177615062761	33.0	31.0	34.0	30.0	34.0
4	35.80048953974895	37.0	35.0	37.0	35.0	37.0
5	31.280771966527197	37.0	35.0	37.0	0.0	37.0
6	33.49182426778243	37.0	35.0	37.0	17.0	37.0
7	29.486667364016736	35.0	32.0	37.0	0.0	37.0
8	32.58208577405858	35.0	33.0	37.0	17.0	37.0
9	36.523119246861924	39.0	35.0	39.0	32.0	39.0
10	36.88387656903766	38.0	37.0	39.0	34.0	39.0
11	36.955127615062764	39.0	37.0	39.0	33.0	39.0
12	36.95211506276151	39.0	37.0	39.0	33.0	39.0
13	36.794115062761506	39.0	37.0	39.0	33.0	39.0
14	38.09426150627615	40.0	37.0	41.0	33.0	41.0
15	38.212861924686194	40.0	37.0	41.0	33.0	41.0
16	38.25674686192469	40.0	37.0	41.0	34.0	41.0
17	38.207495815899584	40.0	37.0	41.0	34.0	41.0
18	38.1344769874477	40.0	37.0	41.0	33.0	41.0
19	38.052667364016735	40.0	37.0	41.0	34.0	41.0
20	37.971223849372386	40.0	36.0	41.0	33.0	41.0
21	37.89395188284519	40.0	36.0	41.0	33.0	41.0
22	37.83823640167364	40.0	36.0	41.0	33.0	41.0
23	37.77492259414226	40.0	36.0	41.0	33.0	41.0
24	37.72218828451883	40.0	36.0	41.0	33.0	41.0
25	37.583123430962345	40.0	36.0	41.0	33.0	41.0
26	37.4198179916318	39.0	35.0	41.0	33.0	41.0
27	37.37460669456067	39.0	35.0	41.0	32.0	41.0
28	37.270280334728035	39.0	35.0	41.0	32.0	41.0
29	37.26976150627615	39.0	35.0	41.0	32.0	41.0
30	37.151910041841006	39.0	35.0	41.0	32.0	41.0
31	37.04029079497908	39.0	35.0	41.0	31.0	41.0
32	36.95460669456067	39.0	35.0	41.0	31.0	41.0
33	36.816640167364014	39.0	35.0	41.0	31.0	41.0
34	36.680539748953976	39.0	35.0	41.0	31.0	41.0
35	36.571012552301255	39.0	35.0	41.0	30.0	41.0
36	36.42797280334728	39.0	35.0	41.0	30.0	41.0
37	36.33146652719665	39.0	35.0	41.0	30.0	41.0
38	36.213617154811715	39.0	35.0	40.0	30.0	41.0
39	36.19038912133891	39.0	35.0	40.0	30.0	41.0
40	36.02204184100418	39.0	35.0	40.0	29.0	41.0
41	35.916822175732214	38.0	35.0	40.0	29.0	41.0
42	35.832322175732216	38.0	35.0	40.0	28.0	41.0
43	35.701953974895396	38.0	35.0	40.0	28.0	41.0
44	35.55794979079498	38.0	34.0	40.0	27.0	41.0
45	35.4204309623431	38.0	34.0	40.0	27.0	41.0
46	35.30356903765691	38.0	34.0	40.0	26.0	41.0
47	35.16060251046025	38.0	34.0	40.0	26.0	41.0
48	35.06048117154812	38.0	34.0	40.0	26.0	41.0
49	34.89439748953975	37.0	34.0	40.0	26.0	41.0
50	34.75215690376569	37.0	34.0	40.0	25.0	41.0
51	32.7949769874477	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	18.0
10	21.0
11	11.0
12	21.0
13	12.0
14	21.0
15	34.0
16	67.0
17	120.0
18	227.0
19	373.0
20	624.0
21	1000.0
22	1392.0
23	2083.0
24	2809.0
25	3755.0
26	4806.0
27	5687.0
28	6666.0
29	7962.0
30	9790.0
31	12648.0
32	16312.0
33	23808.0
34	35226.0
35	41660.0
36	47611.0
37	74565.0
38	91464.0
39	87176.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75774058577406	20.330543933054393	24.878451882845187	12.03326359832636
2	32.483682008368206	23.960041841004184	25.903765690376567	17.652510460251044
3	33.07866108786611	23.74686192468619	26.624686192468623	16.54979079497908
4	28.8826359832636	26.51317991631799	25.921966527196656	18.682217573221756
5	22.534518828451883	40.11171548117155	20.655857740585773	16.697907949790793
6	27.481380753138072	36.40481171548117	22.656066945606696	13.457740585774058
7	72.41046025104603	19.09958158995816	5.363389121338912	3.1265690376569037
8	87.61108786610879	4.140167364016737	5.7215481171548115	2.5271966527196654
9	81.50794979079498	6.869246861924687	7.52887029288703	4.093933054393306
10	45.04748953974895	27.956694560669455	13.923221757322176	13.072594142259414
11	32.09476987447699	27.277405857740582	23.385146443514646	17.242677824267783
12	31.366527196652722	23.233682008368202	26.198535564853557	19.201255230125522
13	24.692677824267783	25.482217573221753	28.17531380753138	21.64979079497908
14	21.463179916317994	26.779079497907947	28.4418410041841	23.315899581589957
15	21.118619246861925	27.703556485355644	29.995397489539748	21.18242677824268
16	24.56945606694561	25.44748953974895	29.732426778242676	20.25062761506276
17	24.622594142259413	26.077615062761506	26.780753138075315	22.519037656903766
18	24.211924686192468	26.75523012552301	27.344351464435146	21.688493723849373
19	25.237656903765693	27.827196652719664	25.667573221757323	21.26757322175732
20	27.049790794979078	26.420083682008368	27.26192468619247	19.268200836820085
21	27.122803347280332	26.933682008368198	26.124058577405858	19.819456066945605
22	25.75502092050209	24.939121338912134	26.452719665271967	22.853138075313808
23	24.872175732217574	26.489539748953977	27.295397489539745	21.342887029288704
24	23.677824267782427	25.360669456066947	29.24330543933054	21.718200836820085
25	23.676778242677823	26.846652719665272	27.182217573221756	22.294351464435145
26	23.32133891213389	27.428661087866107	27.126987447698745	22.123012552301255
27	22.964225941422594	27.70376569037657	27.443933054393305	21.88807531380753
28	21.27866108786611	28.732426778242676	28.469456066945607	21.519456066945605
29	22.68723849372385	26.123012552301255	27.702092050209203	23.487656903765693
30	23.32175732217573	27.640794979079498	27.310460251046027	21.726987447698747
31	24.665899581589958	26.552510460251042	26.184100418410043	22.597489539748956
32	24.677405857740588	27.45355648535565	26.529707112970712	21.339330543933055
33	24.592677824267785	27.168200836820084	26.359414225941425	21.87970711297071
34	22.60836820083682	26.676987447698746	27.123012552301258	23.591631799163178
35	23.92824267782427	25.694769874476986	26.72468619246862	23.652301255230128
36	23.273221757322176	28.31297071129707	26.165062761506274	22.248744769874477
37	23.210460251046026	28.641422594142256	27.252092050209203	20.89602510460251
38	23.538702928870293	27.568410041841002	27.14958158995816	21.743305439330545
39	23.385146443514646	27.829079497907948	27.092468619246862	21.693305439330544
40	24.28661087866109	26.740794979079496	27.481171548117157	21.49142259414226
41	21.57029288702929	26.35	28.76987447698745	23.309832635983263
42	22.08347280334728	26.06778242677824	27.50062761506276	24.348117154811717
43	22.401882845188283	26.648535564853553	27.61129707112971	23.338284518828452
44	22.140794979079498	27.057322175732217	27.047280334728036	23.754602510460252
45	22.556066945606695	27.120711297071132	26.731171548117157	23.59205020920502
46	22.469665271966527	27.841422594142262	26.226987447698747	23.461924686192468
47	22.420920502092052	25.947071129707112	29.064016736401673	22.567991631799163
48	22.852719665271966	25.28347280334728	29.322175732217577	22.54163179916318
49	22.23389121338912	26.697071129707112	27.979288702928873	23.089748953974894
50	21.55502092050209	26.270083682008366	29.25041841004184	22.924476987447697
51	21.818619246861925	26.207322175732216	27.768619246861924	24.205439330543932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	604.0
2	986.0
3	2058.5
4	3131.0
5	2293.0
6	1455.0
7	1191.0
8	927.0
9	903.5
10	880.0
11	928.0
12	976.0
13	967.5
14	959.0
15	972.0
16	985.0
17	1001.5
18	1018.0
19	1097.5
20	1177.0
21	1427.5
22	1678.0
23	1911.0
24	2144.0
25	2674.5
26	3829.5
27	4454.0
28	5943.0
29	7432.0
30	8826.5
31	10221.0
32	11285.5
33	12350.0
34	13437.5
35	14525.0
36	15594.0
37	16663.0
38	18103.5
39	19544.0
40	21619.0
41	23694.0
42	25202.0
43	26710.0
44	28805.0
45	30900.0
46	32194.5
47	33489.0
48	33884.5
49	34280.0
50	33058.0
51	31836.0
52	30302.5
53	28769.0
54	27248.5
55	25728.0
56	25636.0
57	25544.0
58	25615.0
59	25686.0
60	24819.5
61	23953.0
62	21455.5
63	18958.0
64	16052.5
65	13147.0
66	11475.0
67	9803.0
68	8585.5
69	7368.0
70	6336.0
71	5304.0
72	4277.5
73	3251.0
74	2583.5
75	1544.5
76	1173.0
77	939.5
78	706.0
79	544.0
80	382.0
81	312.5
82	243.0
83	172.0
84	101.0
85	87.0
86	73.0
87	49.0
88	25.0
89	20.0
90	15.0
91	9.5
92	4.0
93	5.0
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	478000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.05887561779287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.3008311206353	23.594914672324364
2	9.408059097465186	6.596719474057531
3	5.299951650519392	5.574310370876262
4	3.4322642346240064	4.813252995563284
5	2.4518840804940147	4.298014950364305
6	1.713454818921211	3.6043079623959926
7	1.3481835778284093	3.3086060255526024
8	1.0528599069338782	2.9529667616124655
9	0.9078298358098766	2.8644743966201807
>10	7.0338724664336345	40.24437753179447
>50	0.034072058695335354	0.8154225810478652
>100	0.015541640808398545	0.9243970714474754
>500	5.977554157076364E-4	0.1089744903996117
>1k	5.977554157076364E-4	0.299260715943549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.29874476987447696	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	520	0.10878661087866108	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.276150627615063E-4	0.0	0.0	0.04184100418410042	0.0
2	6.276150627615063E-4	0.0	0.0	0.14246861924686194	0.0
3	6.276150627615063E-4	0.0	0.0	0.20794979079497908	0.0
4	6.276150627615063E-4	0.0	0.0	0.29497907949790797	0.0
5	6.276150627615063E-4	0.0	0.0	0.5527196652719665	0.0
6	6.276150627615063E-4	0.0	0.0	0.7767782426778242	0.0
7	6.276150627615063E-4	0.0	0.0	0.9447698744769875	0.0
8	6.276150627615063E-4	0.0	0.0	1.2577405857740587	0.0
9	6.276150627615063E-4	0.0	0.0	1.395397489539749	0.0
10	6.276150627615063E-4	0.0	0.0	1.7125523012552302	0.0
11	6.276150627615063E-4	0.0	0.0	2.152928870292887	0.0
12	6.276150627615063E-4	0.0	0.0	2.443305439330544	0.0
13	6.276150627615063E-4	0.0	0.0	2.5838912133891214	0.0
14	6.276150627615063E-4	0.0	0.0	2.6391213389121337	0.0
15	6.276150627615063E-4	0.0	0.0	2.757531380753138	0.0
16	6.276150627615063E-4	0.0	0.0	3.0002092050209206	0.0
17	6.276150627615063E-4	0.0	0.0	3.2765690376569037	0.0
18	6.276150627615063E-4	0.0	0.0	3.565062761506276	0.0
19	6.276150627615063E-4	0.0	0.0	3.7640167364016737	0.0
20	6.276150627615063E-4	0.0	0.0	3.9575313807531383	0.0
21	6.276150627615063E-4	0.0	0.0	4.215062761506276	0.0
22	6.276150627615063E-4	0.0	0.0	4.475523012552301	0.0
23	6.276150627615063E-4	0.0	0.0	4.750418410041841	0.0
24	6.276150627615063E-4	0.0	0.0	4.966108786610879	0.0
25	6.276150627615063E-4	0.0	0.0	5.169874476987448	0.0
26	6.276150627615063E-4	0.0	0.0	5.3581589958158995	0.0
27	6.276150627615063E-4	0.0	0.0	5.535983263598326	0.0
28	6.276150627615063E-4	0.0	0.0	5.7320083682008365	0.0
29	6.276150627615063E-4	0.0	0.0	5.940585774058578	0.0
30	6.276150627615063E-4	0.0	0.0	6.177196652719665	0.0
31	6.276150627615063E-4	0.0	0.0	6.398744769874477	0.0
32	6.276150627615063E-4	0.0	0.0	6.6292887029288705	0.0
33	6.276150627615063E-4	0.0	0.0	6.856694560669456	0.0
34	6.276150627615063E-4	0.0	0.0	7.0849372384937235	0.0
35	6.276150627615063E-4	0.0	0.0	7.336192468619247	0.0
36	6.276150627615063E-4	0.0	0.0	7.557322175732217	0.0
37	6.276150627615063E-4	0.0	0.0	7.7889121338912135	0.0
38	6.276150627615063E-4	0.0	0.0	8.025523012552302	0.0
39	6.276150627615063E-4	0.0	0.0	8.266527196652719	2.0920502092050208E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAG	30	2.1628312E-6	45.000004	11
CTTCGCA	25	3.8873746E-5	45.0	24
TCGTTCA	25	3.8873746E-5	45.0	16
ATCCGAC	25	3.8873746E-5	45.0	38
GTATGCG	20	7.0291467E-4	45.0	1
CGAATAT	55	1.8189894E-12	45.0	14
GCGATAT	40	6.8012014E-9	45.0	9
GTATACG	20	7.0291467E-4	45.0	1
TATACGG	35	1.2099372E-7	45.0	2
TTCGCAT	25	3.8873746E-5	45.0	25
TAATCGA	20	7.0291467E-4	45.0	13
ATGGGTA	75	0.0	42.000004	5
GCTACGA	60	3.6379788E-12	41.250004	10
CGGGTAT	120	0.0	39.375004	6
ATAACGG	40	3.4525692E-7	39.375	2
ACCCACC	270	0.0	39.166664	13
ATAGCGG	70	0.0	38.571426	2
ACGGGTA	70	0.0	38.571426	5
ATATCAC	280	0.0	38.571426	30
TTAACGG	35	6.240636E-6	38.571426	2
>>END_MODULE