FastQCFastQC Report
Sat 14 Jan 2017
SRR2936220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632299
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51800.8192326731498863No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.24513718984214747No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.1714378798637986No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.10706959840202183No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG6360.10058532434813276No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG207.0308766E-445.023
CGTACGT207.0308766E-445.024
CCAACGG351.2107739E-745.02
TACGGGT750.042.0000044
CGTTATT4550.041.538461
CGAATAT1150.041.08695614
CGTTTTT17150.040.6705551
TCGCAAC501.0804797E-940.516
AAGGCGA501.0804797E-940.56
CGGTTTT4200.039.6428571
TCGTCCC2600.038.9423138
CGTTTTA2450.038.571431
CGGGTAT700.038.5714266
TACGAAT1250.037.80000312
CGTTGAT2800.037.76785725
ACACGCG3300.037.536
AACCGAC301.1393411E-437.49999641
ACTACGG301.1393411E-437.4999962
TCGTTGA2850.037.10526324
AATCGTT2850.037.10526322
Produced by FastQC (version 0.11.5)