##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	632299
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90278649815989	31.0	31.0	34.0	30.0	34.0
2	32.04754396258732	33.0	31.0	34.0	30.0	34.0
3	31.78007240245517	33.0	31.0	34.0	30.0	34.0
4	35.61371123471649	37.0	35.0	37.0	33.0	37.0
5	31.274370195113388	37.0	35.0	37.0	0.0	37.0
6	33.444947722517355	37.0	35.0	37.0	17.0	37.0
7	29.385864915174626	35.0	32.0	37.0	0.0	37.0
8	32.51542229230159	35.0	33.0	37.0	17.0	37.0
9	36.46109989103257	39.0	35.0	39.0	32.0	39.0
10	36.82852732647055	38.0	37.0	39.0	33.0	39.0
11	36.90587997134267	39.0	37.0	39.0	33.0	39.0
12	36.896900042543166	39.0	37.0	39.0	33.0	39.0
13	36.756877679705326	39.0	35.0	39.0	33.0	39.0
14	37.97593385407853	40.0	37.0	41.0	33.0	41.0
15	38.09721192030985	40.0	37.0	41.0	33.0	41.0
16	38.15491563326844	40.0	37.0	41.0	33.0	41.0
17	38.14037820714567	40.0	37.0	41.0	33.0	41.0
18	38.03662665922293	40.0	37.0	41.0	33.0	41.0
19	37.905154048954685	40.0	37.0	41.0	33.0	41.0
20	37.78519972354851	40.0	36.0	41.0	33.0	41.0
21	37.66970373193695	40.0	36.0	41.0	33.0	41.0
22	37.625802033531606	39.0	35.0	41.0	33.0	41.0
23	37.55490519516874	39.0	35.0	41.0	33.0	41.0
24	37.47955951219281	39.0	35.0	41.0	33.0	41.0
25	37.325233789710246	39.0	35.0	41.0	32.0	41.0
26	37.1730431330747	39.0	35.0	41.0	32.0	41.0
27	37.139565300593546	39.0	35.0	41.0	32.0	41.0
28	37.00708209249105	39.0	35.0	41.0	32.0	41.0
29	36.93684475224538	39.0	35.0	41.0	31.0	41.0
30	36.75610747447015	39.0	35.0	41.0	31.0	41.0
31	36.61202690499273	39.0	35.0	41.0	31.0	41.0
32	36.444106348420604	39.0	35.0	40.0	30.0	41.0
33	36.213585661214076	39.0	35.0	40.0	30.0	41.0
34	36.015684035559126	39.0	35.0	40.0	29.0	41.0
35	35.82893694280712	39.0	35.0	40.0	28.0	41.0
36	35.64054980317856	38.0	35.0	40.0	27.0	41.0
37	35.5214305257481	38.0	35.0	40.0	26.0	41.0
38	35.33708577745655	38.0	34.0	40.0	25.0	41.0
39	35.270272450217384	38.0	34.0	40.0	25.0	41.0
40	35.108921570333024	38.0	34.0	40.0	24.0	41.0
41	34.989512872865525	38.0	34.0	40.0	24.0	41.0
42	34.93657272904117	38.0	34.0	40.0	24.0	41.0
43	34.82190862234481	38.0	34.0	40.0	23.0	41.0
44	34.64341079141356	38.0	34.0	40.0	23.0	41.0
45	34.50298671989043	38.0	33.0	40.0	23.0	41.0
46	34.40310201344617	37.0	33.0	40.0	23.0	41.0
47	34.263726496483464	37.0	33.0	40.0	23.0	41.0
48	34.147593148178316	37.0	33.0	40.0	22.0	41.0
49	33.99337338822298	37.0	33.0	40.0	22.0	41.0
50	33.827124509132545	36.0	33.0	40.0	21.0	41.0
51	32.02829357629855	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	25.0
10	27.0
11	21.0
12	23.0
13	23.0
14	26.0
15	53.0
16	90.0
17	182.0
18	408.0
19	697.0
20	1127.0
21	1798.0
22	2752.0
23	4073.0
24	5497.0
25	7642.0
26	9406.0
27	10582.0
28	11352.0
29	12369.0
30	14752.0
31	17929.0
32	22793.0
33	32861.0
34	46771.0
35	54401.0
36	62872.0
37	95068.0
38	113089.0
39	103560.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71317367258212	20.995446774389965	24.054442597568556	15.236936955459363
2	36.12483967237019	23.564326370909967	23.868138333288524	16.44269562343132
3	32.925245809340204	23.51340109663308	27.272224058554578	16.28912903547214
4	28.796344767269915	25.71457490838986	27.195045382010726	18.2940349423295
5	21.773401507830947	38.248992960608824	23.606869534824508	16.37073599673572
6	27.151078840864844	33.98803414207519	25.865769200963467	12.9951178160965
7	70.50540962424424	18.906087151806343	7.769583693790438	2.818919530158991
8	85.34996892293046	3.804845492401538	8.580750562629389	2.264435022038624
9	79.49783251278272	6.217786205576791	10.365032998628813	3.919348283011676
10	43.315741445107456	27.006685128396533	16.995756754320347	12.681816672175664
11	31.311294182024646	25.23616200563341	26.4259472180092	17.026596594332744
12	29.542827048595683	22.17605911127489	29.526537286948106	18.754576553181327
13	24.4880981940506	23.492050438162956	31.548681873607265	20.47116949417918
14	21.35967319258768	24.692273750235252	31.650374269135327	22.297678788041733
15	20.611767534030577	26.171795305701888	32.53666382518397	20.67977333508356
16	25.169105122734653	25.304167806686394	30.080231029939945	19.446496040639
17	24.674402458330633	25.267159998671513	29.0598277080938	20.998609834904055
18	24.555155076949355	24.683733486847203	30.083394090454043	20.6777173457494
19	24.057921964134056	26.548199506878866	28.106164963094994	21.287713565892087
20	25.93788698068477	25.811206407095376	29.236326484780147	19.01458012743971
21	26.207379736485432	25.8599175390124	29.243443370936852	18.689259353565323
22	26.03182987795331	23.49821840616544	29.75459395001415	20.715357765867097
23	24.734816914149793	25.076269296646046	29.89882950945676	20.290084279747397
24	23.86070514108041	24.56322088126029	31.083237518958594	20.492836458700708
25	23.50486083324503	26.150760953283175	29.224465007852295	21.119913205619493
26	22.88822218602275	26.44413481596523	28.495537712379743	22.17210528563227
27	22.97520635016029	26.000515578863798	29.74431400334335	21.27996406763256
28	21.515453922906726	27.489210009821303	29.623959550782146	21.371376516489825
29	23.410443476899378	25.420252127553578	29.178126171320844	21.991178224226196
30	23.699230901836	25.41345154744828	28.854861386780623	22.032456163935098
31	24.64387892436964	25.40791619154862	27.979642542531302	21.968562341550438
32	24.49458246810449	25.74272614696528	28.022976471574367	21.739714913355865
33	23.662064940795418	26.24185709608903	27.703507359651052	22.392570603464502
34	22.410125589317712	25.585047580337783	30.233006852770604	21.7718199775739
35	23.412499466233538	26.054287607603367	28.498384466842424	22.034828459320668
36	23.430370758138157	27.912901965683957	26.941209775754828	21.715517500423058
37	23.017275054997715	27.55626689272006	27.941211357285084	21.485246694997144
38	23.191401536298493	27.20374379842448	28.454734231747953	21.150120433529075
39	23.283446597258575	27.26668870265491	27.37866104485378	22.07120365523273
40	24.616202144871334	25.85454033613844	28.656695645572743	20.87256187341748
41	21.798389685892275	25.675669264066524	29.067260900301918	23.458680149739287
42	21.910836487168254	25.811680866172495	28.285194188192612	23.992288458466643
43	22.181752620200253	26.325994505763887	28.41551228137321	23.07674059266265
44	22.60623534119143	26.367114292447084	28.567655492101046	22.458994874260437
45	22.71978921364734	25.943738642635843	27.392894817167196	23.943577326549622
46	21.706819084009304	27.576668633035954	27.625063458901565	23.091448824053177
47	22.3171316102034	25.801559072527397	29.572876123479556	22.308433193789647
48	21.704604941649443	26.06504201335128	30.185244639007813	22.04510840599147
49	22.54518827326945	26.70856667494334	28.432434655123608	22.3138103966636
50	21.851845408580434	25.9764763189567	30.00115451708764	22.17052375537523
51	22.02359959449564	25.640242986308692	28.480829480989218	23.85532793820645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	348.0
1	900.5
2	1453.0
3	5894.5
4	10336.0
5	7628.5
6	4921.0
7	3785.5
8	2650.0
9	2521.5
10	2393.0
11	2413.5
12	2434.0
13	2349.0
14	2264.0
15	2245.5
16	2227.0
17	2143.5
18	2060.0
19	2209.0
20	2358.0
21	2496.0
22	2634.0
23	2925.0
24	3216.0
25	3785.5
26	5517.0
27	6679.0
28	8114.0
29	9549.0
30	11027.5
31	12506.0
32	14325.5
33	16145.0
34	18276.5
35	20408.0
36	20918.5
37	21429.0
38	21993.0
39	22557.0
40	25257.5
41	27958.0
42	29975.5
43	31993.0
44	33453.5
45	34914.0
46	37231.0
47	39548.0
48	41028.0
49	42508.0
50	41719.5
51	40931.0
52	39778.5
53	38626.0
54	38186.0
55	37746.0
56	36563.5
57	35381.0
58	34246.5
59	33112.0
60	31262.0
61	29412.0
62	27063.0
63	24714.0
64	22196.5
65	19679.0
66	16932.5
67	14186.0
68	12014.0
69	9842.0
70	8320.0
71	6798.0
72	5672.0
73	4546.0
74	3852.5
75	2602.5
76	2046.0
77	1553.5
78	1061.0
79	812.0
80	563.0
81	435.5
82	308.0
83	235.0
84	162.0
85	135.5
86	109.0
87	72.0
88	35.0
89	28.0
90	21.0
91	16.0
92	11.0
93	6.5
94	2.0
95	3.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	632299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.47059261803751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.12289123530063	25.938939920596393
2	8.500818640325663	6.200597871022735
3	4.428225928599131	4.845000715877093
4	2.8093025186978626	4.098277108010258
5	2.030553515766974	3.702774503133055
6	1.5335796478144537	3.355833514965262
7	1.2467342093384177	3.182839481622677
8	0.9924288503014059	2.8955574641383923
9	0.8500077976548507	2.7900259299382784
>10	6.435452387106615	39.78155322203409
>50	0.032886348148829715	0.7838717746277502
>100	0.014415933427483823	0.833325851859381
>500	0.0013514937588266086	0.30756821661686934
>1k	9.00995839217739E-4	0.43276425350899245
>5k	4.504979196088695E-4	0.8510701720488157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5180	0.8192326731498863	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.24513718984214747	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1084	0.1714378798637986	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	677	0.10706959840202183	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	636	0.10058532434813276	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009647334567981287	0.0
2	0.0	0.0	0.0	0.06294490423043528	0.0
3	0.0	0.0	0.0	0.13537899000314724	0.0
4	0.0	0.0	0.0	0.2119250544441791	0.0
5	0.0	0.0	0.0	0.46987263936840007	0.0
6	0.0	0.0	0.0	0.7475893525056975	0.0
7	0.0	0.0	0.0	0.9476529300220308	0.0
8	0.0	0.0	0.0	1.2508322802977705	0.0
9	0.0	0.0	0.0	1.4339734840637104	0.0
10	0.0	0.0	0.0	1.7521773717813882	0.0
11	0.0	0.0	0.0	2.14218273316896	0.0
12	0.0	0.0	0.0	2.4009210832217036	0.0
13	0.0	0.0	0.0	2.5369326853276695	0.0
14	0.0	0.0	0.0	2.6215445540796365	0.0
15	0.0	0.0	0.0	2.7227624905305876	0.0
16	0.0	0.0	0.0	2.906061847322232	0.0
17	0.0	0.0	0.0	3.0918916525251503	0.0
18	0.0	0.0	0.0	3.310775440100332	0.0
19	0.0	0.0	0.0	3.4543783874401193	0.0
20	0.0	0.0	0.0	3.610000964733457	0.0
21	0.0	0.0	0.0	3.7814388445972553	0.0
22	0.0	0.0	0.0	3.972487699648426	0.0
23	0.0	0.0	0.0	4.1752398786017375	0.0
24	0.0	0.0	0.0	4.337030423897555	0.0
25	0.0	0.0	0.0	4.485219808982776	0.0
26	0.0	0.0	0.0	4.633409194067997	0.0
27	0.0	0.0	0.0	4.798046493826496	0.0
28	0.0	0.0	0.0	4.960153345173723	0.0
29	0.0	0.0	0.0	5.134754285551614	0.0
30	0.0	0.0	0.0	5.410256856329047	0.0
31	0.0	0.0	0.0	5.62265636985034	0.0
32	0.0	0.0	0.0	5.826990079060698	0.0
33	0.0	0.0	0.0	6.028318880782668	0.0
34	0.0	0.0	0.0	6.226326468964841	0.0
35	0.0	0.0	0.0	6.439833053666066	0.0
36	0.0	0.0	0.0	6.635626499488375	0.0
37	0.0	0.0	0.0	6.840592820801551	0.0
38	0.0	0.0	0.0	7.081934338026788	0.0
39	0.0	0.0	0.0	7.421805190265998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	20	7.0308766E-4	45.0	23
CGTACGT	20	7.0308766E-4	45.0	24
CCAACGG	35	1.2107739E-7	45.0	2
TACGGGT	75	0.0	42.000004	4
CGTTATT	455	0.0	41.53846	1
CGAATAT	115	0.0	41.086956	14
CGTTTTT	1715	0.0	40.670555	1
TCGCAAC	50	1.0804797E-9	40.5	16
AAGGCGA	50	1.0804797E-9	40.5	6
CGGTTTT	420	0.0	39.642857	1
TCGTCCC	260	0.0	38.94231	38
CGTTTTA	245	0.0	38.57143	1
CGGGTAT	70	0.0	38.571426	6
TACGAAT	125	0.0	37.800003	12
CGTTGAT	280	0.0	37.767857	25
ACACGCG	330	0.0	37.5	36
AACCGAC	30	1.1393411E-4	37.499996	41
ACTACGG	30	1.1393411E-4	37.499996	2
TCGTTGA	285	0.0	37.105263	24
AATCGTT	285	0.0	37.105263	22
>>END_MODULE