FastQCFastQC Report
Sat 14 Jan 2017
SRR2936219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891701
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.9445991425376893No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.2782322774113744No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.18795537966201675No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.12134112219230438No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC207.0324354E-445.09
TTACGCG207.0324354E-445.01
CGTTTTA4350.043.9655151
CGTTATT6350.043.9370081
CGTTTTT33950.042.349041
CGGTTTT10850.040.2304151
AGTCGTT451.9274012E-840.023
TCGACGG403.457062E-739.3752
TAGGCGA750.039.0000046
ACGACCA8050.038.8509328
ACACGAC7950.038.77358626
CTCGTAG356.2467134E-638.5714261
TAGCACG356.2467134E-638.5714261
GCGATAC356.2467134E-638.5714269
AACACGT7550.038.4437141
CACGACC8150.038.37423327
GCGAGAC8250.038.18181621
AGACACG8200.038.14024724
CGCACGG1300.038.076922
CGGTTTA950.037.8947371
Produced by FastQC (version 0.11.5)