##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936219.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 891701 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848792364256628 31.0 31.0 34.0 30.0 34.0 2 31.968960447504266 33.0 31.0 34.0 30.0 34.0 3 31.580794459129237 33.0 31.0 34.0 30.0 34.0 4 35.48501010989109 37.0 35.0 37.0 33.0 37.0 5 31.032074652826452 37.0 35.0 37.0 0.0 37.0 6 33.33608575071689 37.0 35.0 37.0 17.0 37.0 7 29.70580385129096 35.0 32.0 37.0 0.0 37.0 8 32.76393768763296 35.0 35.0 37.0 17.0 37.0 9 36.66105903211951 39.0 35.0 39.0 32.0 39.0 10 36.88796917352341 38.0 37.0 39.0 33.0 39.0 11 36.99934282904247 39.0 37.0 39.0 33.0 39.0 12 37.125535353218176 39.0 37.0 39.0 34.0 39.0 13 37.05404502181785 39.0 37.0 39.0 33.0 39.0 14 38.34131956788206 40.0 38.0 41.0 34.0 41.0 15 38.403491753401646 40.0 38.0 41.0 34.0 41.0 16 38.41527372964705 40.0 38.0 41.0 34.0 41.0 17 38.3500691375248 40.0 38.0 41.0 34.0 41.0 18 38.23485114404941 40.0 37.0 41.0 34.0 41.0 19 38.1084657301046 40.0 37.0 41.0 34.0 41.0 20 37.97962097160371 40.0 36.0 41.0 34.0 41.0 21 37.88199519794191 40.0 36.0 41.0 33.0 41.0 22 37.84845032135211 40.0 36.0 41.0 33.0 41.0 23 37.769592049352866 40.0 36.0 41.0 33.0 41.0 24 37.72284992390947 40.0 36.0 41.0 33.0 41.0 25 37.600575753531736 39.0 35.0 41.0 33.0 41.0 26 37.46844626169534 39.0 35.0 41.0 33.0 41.0 27 37.44068583527438 39.0 35.0 41.0 33.0 41.0 28 37.32727898701471 39.0 35.0 41.0 33.0 41.0 29 37.296111588974334 39.0 35.0 41.0 33.0 41.0 30 37.15363670109151 39.0 35.0 41.0 32.0 41.0 31 36.99139846204053 39.0 35.0 41.0 31.0 41.0 32 36.82465310681495 39.0 35.0 41.0 31.0 41.0 33 36.57791793437487 39.0 35.0 41.0 30.0 41.0 34 36.33851369461288 39.0 35.0 41.0 30.0 41.0 35 36.13264984563211 39.0 35.0 41.0 29.0 41.0 36 35.930659492363475 39.0 35.0 41.0 27.0 41.0 37 35.83768438075095 39.0 35.0 41.0 27.0 41.0 38 35.64324364332887 39.0 35.0 40.0 26.0 41.0 39 35.62292517334846 39.0 35.0 40.0 26.0 41.0 40 35.517647731694815 39.0 35.0 40.0 25.0 41.0 41 35.440805830654 39.0 35.0 40.0 25.0 41.0 42 35.39604867550894 39.0 35.0 40.0 24.0 41.0 43 35.26397974208844 38.0 35.0 40.0 24.0 41.0 44 35.11600188852541 38.0 34.0 40.0 24.0 41.0 45 34.98083326137349 38.0 34.0 40.0 23.0 41.0 46 34.89757777550995 38.0 34.0 40.0 23.0 41.0 47 34.742530287618834 38.0 34.0 40.0 23.0 41.0 48 34.65771710472457 38.0 34.0 40.0 23.0 41.0 49 34.526005914538615 38.0 34.0 40.0 22.0 41.0 50 34.33334604312432 38.0 33.0 40.0 21.0 41.0 51 32.607530999740945 36.0 31.0 39.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 19.0 9 34.0 10 31.0 11 21.0 12 12.0 13 13.0 14 12.0 15 48.0 16 95.0 17 183.0 18 331.0 19 620.0 20 1159.0 21 1953.0 22 3204.0 23 5056.0 24 7854.0 25 11200.0 26 14000.0 27 15118.0 28 15426.0 29 16407.0 30 18516.0 31 22643.0 32 28564.0 33 38461.0 34 55207.0 35 69245.0 36 84927.0 37 135776.0 38 174981.0 39 170563.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.90496702369965 19.481305953453006 23.516515065027402 16.097211957819944 2 35.95465296102617 22.58985915682499 24.530419950185095 16.925067931963742 3 31.706480086934967 23.196789058215703 28.95993163627718 16.136799218572147 4 25.566978168691072 25.83926674972889 30.153941736075208 18.439813345504827 5 19.79497611867655 39.01229223697181 25.183553679989146 16.009177964362493 6 24.826819752360937 33.73619632589848 28.306573616043945 13.13041030569664 7 70.6860259212449 17.522353344899244 9.083313801375125 2.7083069324807307 8 86.01627675644639 2.4417377573872856 9.396759676169477 2.1452258099968486 9 80.43705233032149 5.31254310581686 10.935616310848593 3.3147882530130617 10 33.97170127654898 35.701765502113375 18.081845820516072 12.244687400821576 11 23.504627672280282 26.455168268287238 32.76838312393953 17.27182093549295 12 22.911491632284815 23.83960542827697 33.71410371862317 19.53479922081505 13 21.404932819409197 25.34919216194666 34.715784775389956 18.530090243254186 14 19.14834681131904 26.6098165192144 33.941758504251986 20.300078165214572 15 19.73452984800959 27.024978103646852 34.32753804246042 18.912954005883137 16 23.2315540747403 27.184448598801612 32.16268681990936 17.42131050654872 17 22.023413677903246 26.049763317524594 32.48622576401731 19.44059724055485 18 23.058962589477865 24.78027948830381 32.224927414009855 19.93583050820847 19 21.098888528778144 28.004342262709137 30.926734409852628 19.970034798660087 20 22.987750378209736 27.775341734505176 30.780384904805537 18.456522982479555 21 22.96980714387446 26.94501856564028 32.26462681997665 17.820547470508615 22 22.6861918961625 24.250729784983978 31.933349856061614 21.1297284627919 23 20.7752374394556 27.1724490608399 32.440582661676956 19.611730838027544 24 20.184568594181236 26.30769731109419 34.352882860959 19.15485123376558 25 21.21372522852391 27.67956972124064 31.694256258544062 19.412448791691386 26 20.815048990636996 28.329787675465205 30.64468919514501 20.210474138752787 27 20.627654337047957 26.750558763531725 31.75952477343863 20.86226212598169 28 19.004015920134666 28.71982873182827 31.77488866783821 20.501266680198857 29 19.729707603781986 25.871115990674003 31.799112034190834 22.600064371353177 30 21.364784832584014 25.927525033615527 31.92684543361508 20.780844700185376 31 21.28168522856877 26.59714410996511 31.110203980930827 21.010966680535294 32 20.472781795691606 26.43756146959575 31.50843163795936 21.581225096753283 33 20.246921333496317 26.11817189842784 30.41916516859351 23.215741599482335 34 18.798341596566562 27.330573813419523 33.057381341952066 20.813703248061852 35 19.63449631659043 27.358946552712176 31.740572232171992 21.265984898525403 36 20.657036383271972 29.664540019580553 29.502714474919284 20.17570912222819 37 20.310619815386545 27.887935529959034 30.464135399646292 21.337309255008126 38 19.92293380852999 28.6993061575573 31.068373815886712 20.309386218026 39 20.977210970942053 28.131627081275003 29.251509194225417 21.639652753557527 40 22.38182978375038 27.542864704648757 30.11996173605278 19.95534377554808 41 20.059078099048897 27.562265826773775 30.03114272609316 22.347513348084167 42 20.85979493126059 27.64715975422255 29.908231570896522 21.58481374362034 43 20.9439038422072 27.24893209719401 29.50103229670035 22.30613176389844 44 21.00625658152228 27.94423242768596 29.169531042356127 21.87997994843563 45 20.849365426303212 26.454831832643457 29.14261619085321 23.553186550200124 46 21.16909143311491 27.189046552600033 28.561479688819457 23.0803823254656 47 19.79026601966354 27.38731929200483 30.77062827113573 22.0517864171959 48 20.43790463395241 27.3904593580135 30.47041553166364 21.70122047637044 49 21.18524034401666 27.30186463848308 29.805394409112473 21.70750060838779 50 20.195670970426185 26.83556483619509 31.241413882007535 21.72735031137119 51 20.206324765812756 26.17088014928771 29.931221339888598 23.691573745010942 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 213.0 1 524.0 2 835.0 3 8922.0 4 17009.0 5 12819.0 6 8629.0 7 6722.5 8 4816.0 9 4686.0 10 4556.0 11 4602.0 12 4648.0 13 4483.5 14 4319.0 15 4252.5 16 4186.0 17 3997.0 18 3808.0 19 3943.0 20 4078.0 21 4337.0 22 4596.0 23 4956.5 24 5317.0 25 6430.0 26 9256.0 27 10969.0 28 13209.0 29 15449.0 30 17526.5 31 19604.0 32 22625.0 33 25646.0 34 28982.0 35 32318.0 36 35273.0 37 38228.0 38 40480.5 39 42733.0 40 45398.5 41 48064.0 42 51113.0 43 54162.0 44 57247.5 45 60333.0 46 65602.5 47 70872.0 48 72022.0 49 73172.0 50 71463.0 51 69754.0 52 65185.5 53 60617.0 54 55271.5 55 49926.0 56 44577.0 57 39228.0 58 35221.0 59 31214.0 60 27632.5 61 24051.0 62 20585.0 63 17119.0 64 14474.5 65 11830.0 66 9332.5 67 6835.0 68 5529.0 69 4223.0 70 3681.5 71 3140.0 72 2782.5 73 2425.0 74 2309.0 75 1901.0 76 1609.0 77 1154.0 78 699.0 79 534.0 80 369.0 81 269.5 82 170.0 83 134.0 84 98.0 85 75.0 86 52.0 87 38.5 88 25.0 89 19.0 90 13.0 91 8.0 92 3.0 93 2.5 94 2.0 95 2.0 96 2.0 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 891701.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.45596942236757 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48396604780432 27.489852125239246 2 9.369305231558231 7.206114309872637 3 4.5876360284087365 5.292659724883147 4 2.8896662204624803 4.444996632598145 5 2.0068886728101027 3.858842471784058 6 1.460125961276167 3.3690335591784804 7 1.1070348561658552 2.9800469004746453 8 0.8698684068863738 2.6761306285364785 9 0.7521230804770014 2.6031259966212428 >10 5.414091608979914 36.151705499229536 >50 0.04188932311433875 1.052865402486982 >100 0.015634606593278023 1.153480851836421 >500 5.899851544190171E-4 0.17129780548781343 >1k 8.849777316285257E-4 0.5943239754640096 >5k 2.9499257720950854E-4 0.9555241163071871 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8423 0.9445991425376893 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2481 0.2782322774113744 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1676 0.18795537966201675 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.12134112219230438 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.2429042919095077E-4 0.0 0.0 0.052708250859873435 0.0 2 3.364356437864262E-4 0.0 0.0 0.17517082519813257 0.0 3 3.364356437864262E-4 0.0 0.0 0.28182092427842964 0.0 4 3.364356437864262E-4 0.0 0.0 0.4270489771795703 0.0 5 3.364356437864262E-4 0.0 0.0 0.8922273273216021 0.0 6 3.364356437864262E-4 0.0 0.0 1.5262963706444201 0.0 7 3.364356437864262E-4 0.0 0.0 1.972410034305221 0.0 8 3.364356437864262E-4 0.0 0.0 2.623525150246551 0.0 9 3.364356437864262E-4 0.0 0.0 2.9668016521233014 0.0 10 3.364356437864262E-4 0.0 0.0 3.483791091408443 0.0 11 3.364356437864262E-4 0.0 0.0 4.0224245571105115 0.0 12 3.364356437864262E-4 0.0 0.0 4.413587065619529 0.0 13 3.364356437864262E-4 0.0 0.0 4.625877956848765 0.0 14 3.364356437864262E-4 0.0 0.0 4.761012940436312 0.0 15 3.364356437864262E-4 0.0 0.0 4.896260069238456 0.0 16 3.364356437864262E-4 0.0 0.0 5.1300828416700215 0.0 17 3.364356437864262E-4 0.0 0.0 5.433884228009164 0.0 18 3.364356437864262E-4 0.0 0.0 5.7702077265809955 0.0 19 3.364356437864262E-4 0.0 0.0 5.996292479205474 0.0 20 4.4858085838190155E-4 0.0 0.0 6.218676439748301 0.0 21 4.4858085838190155E-4 0.0 0.0 6.499151621451586 0.0 22 4.4858085838190155E-4 0.0 0.0 6.809681720666457 0.0 23 4.4858085838190155E-4 0.0 0.0 7.134342116920358 0.0 24 4.4858085838190155E-4 0.0 0.0 7.397098354717556 0.0 25 4.4858085838190155E-4 0.0 0.0 7.639780599102165 0.0 26 4.4858085838190155E-4 0.0 0.0 7.8594730744947015 0.0 27 4.4858085838190155E-4 0.0 0.0 8.097669510295491 0.0 28 5.60726072977377E-4 0.0 0.0 8.337548124315214 0.0 29 5.60726072977377E-4 0.0 0.0 8.60086508818539 0.0 30 5.60726072977377E-4 0.0 0.0 8.983840996028938 0.0 31 5.60726072977377E-4 0.0 0.0 9.27654000612313 0.0 32 5.60726072977377E-4 0.0 0.0 9.56452891720431 0.0 33 5.60726072977377E-4 0.0 0.0 9.848256310130862 0.0 34 5.60726072977377E-4 0.0 0.0 10.117292679945407 0.0 35 5.60726072977377E-4 0.0 0.0 10.413692482121249 0.0 36 5.60726072977377E-4 0.0 0.0 10.681943835433627 0.0 37 5.60726072977377E-4 0.0 0.0 10.965559083145584 0.0 38 5.60726072977377E-4 0.0 0.0 11.272612680707995 0.0 39 5.60726072977377E-4 0.0 0.0 11.650093473036366 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGATC 20 7.0324354E-4 45.0 9 TTACGCG 20 7.0324354E-4 45.0 1 CGTTTTA 435 0.0 43.965515 1 CGTTATT 635 0.0 43.937008 1 CGTTTTT 3395 0.0 42.34904 1 CGGTTTT 1085 0.0 40.230415 1 AGTCGTT 45 1.9274012E-8 40.0 23 TCGACGG 40 3.457062E-7 39.375 2 TAGGCGA 75 0.0 39.000004 6 ACGACCA 805 0.0 38.85093 28 ACACGAC 795 0.0 38.773586 26 CTCGTAG 35 6.2467134E-6 38.571426 1 TAGCACG 35 6.2467134E-6 38.571426 1 GCGATAC 35 6.2467134E-6 38.571426 9 AACACGT 755 0.0 38.44371 41 CACGACC 815 0.0 38.374233 27 GCGAGAC 825 0.0 38.181816 21 AGACACG 820 0.0 38.140247 24 CGCACGG 130 0.0 38.07692 2 CGGTTTA 95 0.0 37.894737 1 >>END_MODULE