##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936219.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891701
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848792364256628	31.0	31.0	34.0	30.0	34.0
2	31.968960447504266	33.0	31.0	34.0	30.0	34.0
3	31.580794459129237	33.0	31.0	34.0	30.0	34.0
4	35.48501010989109	37.0	35.0	37.0	33.0	37.0
5	31.032074652826452	37.0	35.0	37.0	0.0	37.0
6	33.33608575071689	37.0	35.0	37.0	17.0	37.0
7	29.70580385129096	35.0	32.0	37.0	0.0	37.0
8	32.76393768763296	35.0	35.0	37.0	17.0	37.0
9	36.66105903211951	39.0	35.0	39.0	32.0	39.0
10	36.88796917352341	38.0	37.0	39.0	33.0	39.0
11	36.99934282904247	39.0	37.0	39.0	33.0	39.0
12	37.125535353218176	39.0	37.0	39.0	34.0	39.0
13	37.05404502181785	39.0	37.0	39.0	33.0	39.0
14	38.34131956788206	40.0	38.0	41.0	34.0	41.0
15	38.403491753401646	40.0	38.0	41.0	34.0	41.0
16	38.41527372964705	40.0	38.0	41.0	34.0	41.0
17	38.3500691375248	40.0	38.0	41.0	34.0	41.0
18	38.23485114404941	40.0	37.0	41.0	34.0	41.0
19	38.1084657301046	40.0	37.0	41.0	34.0	41.0
20	37.97962097160371	40.0	36.0	41.0	34.0	41.0
21	37.88199519794191	40.0	36.0	41.0	33.0	41.0
22	37.84845032135211	40.0	36.0	41.0	33.0	41.0
23	37.769592049352866	40.0	36.0	41.0	33.0	41.0
24	37.72284992390947	40.0	36.0	41.0	33.0	41.0
25	37.600575753531736	39.0	35.0	41.0	33.0	41.0
26	37.46844626169534	39.0	35.0	41.0	33.0	41.0
27	37.44068583527438	39.0	35.0	41.0	33.0	41.0
28	37.32727898701471	39.0	35.0	41.0	33.0	41.0
29	37.296111588974334	39.0	35.0	41.0	33.0	41.0
30	37.15363670109151	39.0	35.0	41.0	32.0	41.0
31	36.99139846204053	39.0	35.0	41.0	31.0	41.0
32	36.82465310681495	39.0	35.0	41.0	31.0	41.0
33	36.57791793437487	39.0	35.0	41.0	30.0	41.0
34	36.33851369461288	39.0	35.0	41.0	30.0	41.0
35	36.13264984563211	39.0	35.0	41.0	29.0	41.0
36	35.930659492363475	39.0	35.0	41.0	27.0	41.0
37	35.83768438075095	39.0	35.0	41.0	27.0	41.0
38	35.64324364332887	39.0	35.0	40.0	26.0	41.0
39	35.62292517334846	39.0	35.0	40.0	26.0	41.0
40	35.517647731694815	39.0	35.0	40.0	25.0	41.0
41	35.440805830654	39.0	35.0	40.0	25.0	41.0
42	35.39604867550894	39.0	35.0	40.0	24.0	41.0
43	35.26397974208844	38.0	35.0	40.0	24.0	41.0
44	35.11600188852541	38.0	34.0	40.0	24.0	41.0
45	34.98083326137349	38.0	34.0	40.0	23.0	41.0
46	34.89757777550995	38.0	34.0	40.0	23.0	41.0
47	34.742530287618834	38.0	34.0	40.0	23.0	41.0
48	34.65771710472457	38.0	34.0	40.0	23.0	41.0
49	34.526005914538615	38.0	34.0	40.0	22.0	41.0
50	34.33334604312432	38.0	33.0	40.0	21.0	41.0
51	32.607530999740945	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	19.0
9	34.0
10	31.0
11	21.0
12	12.0
13	13.0
14	12.0
15	48.0
16	95.0
17	183.0
18	331.0
19	620.0
20	1159.0
21	1953.0
22	3204.0
23	5056.0
24	7854.0
25	11200.0
26	14000.0
27	15118.0
28	15426.0
29	16407.0
30	18516.0
31	22643.0
32	28564.0
33	38461.0
34	55207.0
35	69245.0
36	84927.0
37	135776.0
38	174981.0
39	170563.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90496702369965	19.481305953453006	23.516515065027402	16.097211957819944
2	35.95465296102617	22.58985915682499	24.530419950185095	16.925067931963742
3	31.706480086934967	23.196789058215703	28.95993163627718	16.136799218572147
4	25.566978168691072	25.83926674972889	30.153941736075208	18.439813345504827
5	19.79497611867655	39.01229223697181	25.183553679989146	16.009177964362493
6	24.826819752360937	33.73619632589848	28.306573616043945	13.13041030569664
7	70.6860259212449	17.522353344899244	9.083313801375125	2.7083069324807307
8	86.01627675644639	2.4417377573872856	9.396759676169477	2.1452258099968486
9	80.43705233032149	5.31254310581686	10.935616310848593	3.3147882530130617
10	33.97170127654898	35.701765502113375	18.081845820516072	12.244687400821576
11	23.504627672280282	26.455168268287238	32.76838312393953	17.27182093549295
12	22.911491632284815	23.83960542827697	33.71410371862317	19.53479922081505
13	21.404932819409197	25.34919216194666	34.715784775389956	18.530090243254186
14	19.14834681131904	26.6098165192144	33.941758504251986	20.300078165214572
15	19.73452984800959	27.024978103646852	34.32753804246042	18.912954005883137
16	23.2315540747403	27.184448598801612	32.16268681990936	17.42131050654872
17	22.023413677903246	26.049763317524594	32.48622576401731	19.44059724055485
18	23.058962589477865	24.78027948830381	32.224927414009855	19.93583050820847
19	21.098888528778144	28.004342262709137	30.926734409852628	19.970034798660087
20	22.987750378209736	27.775341734505176	30.780384904805537	18.456522982479555
21	22.96980714387446	26.94501856564028	32.26462681997665	17.820547470508615
22	22.6861918961625	24.250729784983978	31.933349856061614	21.1297284627919
23	20.7752374394556	27.1724490608399	32.440582661676956	19.611730838027544
24	20.184568594181236	26.30769731109419	34.352882860959	19.15485123376558
25	21.21372522852391	27.67956972124064	31.694256258544062	19.412448791691386
26	20.815048990636996	28.329787675465205	30.64468919514501	20.210474138752787
27	20.627654337047957	26.750558763531725	31.75952477343863	20.86226212598169
28	19.004015920134666	28.71982873182827	31.77488866783821	20.501266680198857
29	19.729707603781986	25.871115990674003	31.799112034190834	22.600064371353177
30	21.364784832584014	25.927525033615527	31.92684543361508	20.780844700185376
31	21.28168522856877	26.59714410996511	31.110203980930827	21.010966680535294
32	20.472781795691606	26.43756146959575	31.50843163795936	21.581225096753283
33	20.246921333496317	26.11817189842784	30.41916516859351	23.215741599482335
34	18.798341596566562	27.330573813419523	33.057381341952066	20.813703248061852
35	19.63449631659043	27.358946552712176	31.740572232171992	21.265984898525403
36	20.657036383271972	29.664540019580553	29.502714474919284	20.17570912222819
37	20.310619815386545	27.887935529959034	30.464135399646292	21.337309255008126
38	19.92293380852999	28.6993061575573	31.068373815886712	20.309386218026
39	20.977210970942053	28.131627081275003	29.251509194225417	21.639652753557527
40	22.38182978375038	27.542864704648757	30.11996173605278	19.95534377554808
41	20.059078099048897	27.562265826773775	30.03114272609316	22.347513348084167
42	20.85979493126059	27.64715975422255	29.908231570896522	21.58481374362034
43	20.9439038422072	27.24893209719401	29.50103229670035	22.30613176389844
44	21.00625658152228	27.94423242768596	29.169531042356127	21.87997994843563
45	20.849365426303212	26.454831832643457	29.14261619085321	23.553186550200124
46	21.16909143311491	27.189046552600033	28.561479688819457	23.0803823254656
47	19.79026601966354	27.38731929200483	30.77062827113573	22.0517864171959
48	20.43790463395241	27.3904593580135	30.47041553166364	21.70122047637044
49	21.18524034401666	27.30186463848308	29.805394409112473	21.70750060838779
50	20.195670970426185	26.83556483619509	31.241413882007535	21.72735031137119
51	20.206324765812756	26.17088014928771	29.931221339888598	23.691573745010942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	213.0
1	524.0
2	835.0
3	8922.0
4	17009.0
5	12819.0
6	8629.0
7	6722.5
8	4816.0
9	4686.0
10	4556.0
11	4602.0
12	4648.0
13	4483.5
14	4319.0
15	4252.5
16	4186.0
17	3997.0
18	3808.0
19	3943.0
20	4078.0
21	4337.0
22	4596.0
23	4956.5
24	5317.0
25	6430.0
26	9256.0
27	10969.0
28	13209.0
29	15449.0
30	17526.5
31	19604.0
32	22625.0
33	25646.0
34	28982.0
35	32318.0
36	35273.0
37	38228.0
38	40480.5
39	42733.0
40	45398.5
41	48064.0
42	51113.0
43	54162.0
44	57247.5
45	60333.0
46	65602.5
47	70872.0
48	72022.0
49	73172.0
50	71463.0
51	69754.0
52	65185.5
53	60617.0
54	55271.5
55	49926.0
56	44577.0
57	39228.0
58	35221.0
59	31214.0
60	27632.5
61	24051.0
62	20585.0
63	17119.0
64	14474.5
65	11830.0
66	9332.5
67	6835.0
68	5529.0
69	4223.0
70	3681.5
71	3140.0
72	2782.5
73	2425.0
74	2309.0
75	1901.0
76	1609.0
77	1154.0
78	699.0
79	534.0
80	369.0
81	269.5
82	170.0
83	134.0
84	98.0
85	75.0
86	52.0
87	38.5
88	25.0
89	19.0
90	13.0
91	8.0
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	891701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.45596942236757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48396604780432	27.489852125239246
2	9.369305231558231	7.206114309872637
3	4.5876360284087365	5.292659724883147
4	2.8896662204624803	4.444996632598145
5	2.0068886728101027	3.858842471784058
6	1.460125961276167	3.3690335591784804
7	1.1070348561658552	2.9800469004746453
8	0.8698684068863738	2.6761306285364785
9	0.7521230804770014	2.6031259966212428
>10	5.414091608979914	36.151705499229536
>50	0.04188932311433875	1.052865402486982
>100	0.015634606593278023	1.153480851836421
>500	5.899851544190171E-4	0.17129780548781343
>1k	8.849777316285257E-4	0.5943239754640096
>5k	2.9499257720950854E-4	0.9555241163071871
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8423	0.9445991425376893	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2481	0.2782322774113744	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1676	0.18795537966201675	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1082	0.12134112219230438	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2429042919095077E-4	0.0	0.0	0.052708250859873435	0.0
2	3.364356437864262E-4	0.0	0.0	0.17517082519813257	0.0
3	3.364356437864262E-4	0.0	0.0	0.28182092427842964	0.0
4	3.364356437864262E-4	0.0	0.0	0.4270489771795703	0.0
5	3.364356437864262E-4	0.0	0.0	0.8922273273216021	0.0
6	3.364356437864262E-4	0.0	0.0	1.5262963706444201	0.0
7	3.364356437864262E-4	0.0	0.0	1.972410034305221	0.0
8	3.364356437864262E-4	0.0	0.0	2.623525150246551	0.0
9	3.364356437864262E-4	0.0	0.0	2.9668016521233014	0.0
10	3.364356437864262E-4	0.0	0.0	3.483791091408443	0.0
11	3.364356437864262E-4	0.0	0.0	4.0224245571105115	0.0
12	3.364356437864262E-4	0.0	0.0	4.413587065619529	0.0
13	3.364356437864262E-4	0.0	0.0	4.625877956848765	0.0
14	3.364356437864262E-4	0.0	0.0	4.761012940436312	0.0
15	3.364356437864262E-4	0.0	0.0	4.896260069238456	0.0
16	3.364356437864262E-4	0.0	0.0	5.1300828416700215	0.0
17	3.364356437864262E-4	0.0	0.0	5.433884228009164	0.0
18	3.364356437864262E-4	0.0	0.0	5.7702077265809955	0.0
19	3.364356437864262E-4	0.0	0.0	5.996292479205474	0.0
20	4.4858085838190155E-4	0.0	0.0	6.218676439748301	0.0
21	4.4858085838190155E-4	0.0	0.0	6.499151621451586	0.0
22	4.4858085838190155E-4	0.0	0.0	6.809681720666457	0.0
23	4.4858085838190155E-4	0.0	0.0	7.134342116920358	0.0
24	4.4858085838190155E-4	0.0	0.0	7.397098354717556	0.0
25	4.4858085838190155E-4	0.0	0.0	7.639780599102165	0.0
26	4.4858085838190155E-4	0.0	0.0	7.8594730744947015	0.0
27	4.4858085838190155E-4	0.0	0.0	8.097669510295491	0.0
28	5.60726072977377E-4	0.0	0.0	8.337548124315214	0.0
29	5.60726072977377E-4	0.0	0.0	8.60086508818539	0.0
30	5.60726072977377E-4	0.0	0.0	8.983840996028938	0.0
31	5.60726072977377E-4	0.0	0.0	9.27654000612313	0.0
32	5.60726072977377E-4	0.0	0.0	9.56452891720431	0.0
33	5.60726072977377E-4	0.0	0.0	9.848256310130862	0.0
34	5.60726072977377E-4	0.0	0.0	10.117292679945407	0.0
35	5.60726072977377E-4	0.0	0.0	10.413692482121249	0.0
36	5.60726072977377E-4	0.0	0.0	10.681943835433627	0.0
37	5.60726072977377E-4	0.0	0.0	10.965559083145584	0.0
38	5.60726072977377E-4	0.0	0.0	11.272612680707995	0.0
39	5.60726072977377E-4	0.0	0.0	11.650093473036366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATC	20	7.0324354E-4	45.0	9
TTACGCG	20	7.0324354E-4	45.0	1
CGTTTTA	435	0.0	43.965515	1
CGTTATT	635	0.0	43.937008	1
CGTTTTT	3395	0.0	42.34904	1
CGGTTTT	1085	0.0	40.230415	1
AGTCGTT	45	1.9274012E-8	40.0	23
TCGACGG	40	3.457062E-7	39.375	2
TAGGCGA	75	0.0	39.000004	6
ACGACCA	805	0.0	38.85093	28
ACACGAC	795	0.0	38.773586	26
CTCGTAG	35	6.2467134E-6	38.571426	1
TAGCACG	35	6.2467134E-6	38.571426	1
GCGATAC	35	6.2467134E-6	38.571426	9
AACACGT	755	0.0	38.44371	41
CACGACC	815	0.0	38.374233	27
GCGAGAC	825	0.0	38.181816	21
AGACACG	820	0.0	38.140247	24
CGCACGG	130	0.0	38.07692	2
CGGTTTA	95	0.0	37.894737	1
>>END_MODULE