##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615363
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98161735430957	33.0	31.0	34.0	30.0	34.0
2	32.11646296576167	33.0	31.0	34.0	30.0	34.0
3	31.92444134600228	33.0	31.0	34.0	30.0	34.0
4	35.69842678224072	37.0	35.0	37.0	35.0	37.0
5	31.151530072493795	37.0	35.0	37.0	0.0	37.0
6	33.443424450283814	37.0	35.0	37.0	17.0	37.0
7	29.694814280351597	35.0	32.0	37.0	0.0	37.0
8	32.78562572010342	35.0	35.0	37.0	17.0	37.0
9	36.718068847168254	39.0	35.0	39.0	32.0	39.0
10	36.9272608200363	39.0	37.0	39.0	34.0	39.0
11	37.03367280775737	39.0	37.0	39.0	34.0	39.0
12	37.16126741451794	39.0	37.0	39.0	34.0	39.0
13	37.04801718660368	39.0	37.0	39.0	33.0	39.0
14	38.34824160698645	40.0	38.0	41.0	34.0	41.0
15	38.4282220412992	40.0	38.0	41.0	34.0	41.0
16	38.45314554173715	40.0	38.0	41.0	34.0	41.0
17	38.4264799801093	40.0	38.0	41.0	34.0	41.0
18	38.344718483236726	40.0	37.0	41.0	34.0	41.0
19	38.23036809167922	40.0	37.0	41.0	34.0	41.0
20	38.16997122023911	40.0	37.0	41.0	34.0	41.0
21	38.1032935031843	40.0	37.0	41.0	34.0	41.0
22	38.079358037451065	40.0	37.0	41.0	34.0	41.0
23	38.01011760538089	40.0	37.0	41.0	34.0	41.0
24	37.96258956095833	40.0	36.0	41.0	33.0	41.0
25	37.84911182505285	40.0	36.0	41.0	33.0	41.0
26	37.72929961664904	40.0	36.0	41.0	33.0	41.0
27	37.72340390956232	40.0	36.0	41.0	33.0	41.0
28	37.6546493695591	40.0	36.0	41.0	33.0	41.0
29	37.65093611413101	40.0	36.0	41.0	33.0	41.0
30	37.55848174167117	40.0	36.0	41.0	33.0	41.0
31	37.44298243475802	40.0	36.0	41.0	33.0	41.0
32	37.316834128798774	40.0	36.0	41.0	32.0	41.0
33	37.15896633369247	39.0	36.0	41.0	32.0	41.0
34	37.028074486116324	39.0	35.0	41.0	31.0	41.0
35	36.903853497854115	39.0	35.0	41.0	31.0	41.0
36	36.81806348447989	39.0	35.0	41.0	31.0	41.0
37	36.701173128706145	39.0	35.0	41.0	30.0	41.0
38	36.609246899797355	39.0	35.0	41.0	30.0	41.0
39	36.60291242729901	39.0	35.0	41.0	30.0	41.0
40	36.469264482914966	39.0	35.0	41.0	30.0	41.0
41	36.40110308874599	39.0	35.0	41.0	30.0	41.0
42	36.34244502838162	39.0	35.0	41.0	30.0	41.0
43	36.204155920976724	39.0	35.0	40.0	30.0	41.0
44	36.06467564673209	39.0	35.0	40.0	29.0	41.0
45	35.96135451757743	39.0	35.0	40.0	29.0	41.0
46	35.849105324824535	39.0	35.0	40.0	28.0	41.0
47	35.69973007801899	38.0	35.0	40.0	27.0	41.0
48	35.59788937586433	38.0	35.0	40.0	27.0	41.0
49	35.45381669031125	38.0	35.0	40.0	26.0	41.0
50	35.273168845055686	38.0	34.0	40.0	26.0	41.0
51	33.39854524890187	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	21.0
10	28.0
11	18.0
12	11.0
13	9.0
14	7.0
15	18.0
16	36.0
17	86.0
18	191.0
19	301.0
20	530.0
21	890.0
22	1481.0
23	2267.0
24	3603.0
25	4922.0
26	6066.0
27	6855.0
28	7772.0
29	8814.0
30	10976.0
31	13958.0
32	18616.0
33	26426.0
34	39409.0
35	50486.0
36	58756.0
37	96255.0
38	127300.0
39	129230.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95972621038314	20.853869992183473	26.293748567918446	11.892655229514936
2	33.227704623124886	24.46182172148797	25.65770122675559	16.652772428631557
3	31.185007873401556	24.20538771424346	28.18450248064963	16.425101931705353
4	26.253772163747254	26.582846222473567	28.543965106774376	18.619416507004807
5	21.4419781494825	39.75052123705845	22.5163033851564	16.291197228302646
6	25.70060273367102	36.107143263407124	25.186597179225924	13.005656823695933
7	72.73056716117154	17.97638142039739	6.571243314921436	2.7218081035096358
8	88.09353178530395	3.4038445600401714	6.30115882820384	2.201464826452029
9	82.59547616610034	5.947383901859553	7.616480028861014	3.840659903179099
10	36.03889086604167	35.99436430204611	15.780116776601776	12.186628055310443
11	24.89554945617465	26.65808636528358	31.37237695474053	17.073987223801236
12	24.672754130488833	23.88330140096171	32.79413939414622	18.64980507440324
13	21.468466579888617	26.257672300739564	34.352731639698845	17.921129479672974
14	19.463146143008274	28.580366385369288	32.229269553093054	19.727217918529387
15	18.5393011929544	28.57419116846479	35.0350931076454	17.8514145309354
16	21.39728907977893	26.738851702166038	33.41897384145618	18.44488537659885
17	21.699712202391108	26.224683642012923	30.857071354631334	21.218532800964635
18	22.186741809306053	26.329987340805346	31.912383422467716	19.57088742742089
19	21.370313132248768	29.29620402916652	29.9462268612185	19.387255977366205
20	23.594853769238643	27.66204662938786	30.85934643454351	17.883753166829987
21	22.87722856265326	27.981045334217363	30.877547073840965	18.264179029288403
22	21.784702687681907	25.582298578237562	30.38547978997762	22.24751894410291
23	20.095455852886833	28.689895232570045	31.33613818185364	19.878510732689485
24	20.750191350471187	26.974322473076867	32.88368003926138	19.391806137190567
25	20.27843728010946	29.37566932038488	30.1763349437649	20.16955845574076
26	19.80749573828781	30.148708973402687	30.454382210175133	19.589413078134367
27	19.69471677692679	29.882037106553366	31.428603929713034	18.99464218680681
28	17.99913221951921	28.528852075929166	33.740572637613894	19.731443066937725
29	19.312340845972216	26.516056376480222	33.12630106132478	21.045301716222784
30	20.530808644653646	27.509941286687695	32.449139776034635	19.510110292624027
31	21.460503800195983	27.59200666923426	30.39360507537827	20.553884455191486
32	21.048876841799068	29.00190619195499	29.782746118957427	20.16647084728851
33	21.783077630601774	28.01224643015586	29.413208138935882	20.791467800306485
34	19.44787060645505	27.825364865940916	30.903222975707024	21.823541551897012
35	19.375718072097282	27.939768884382065	31.077104083280926	21.60740896023973
36	21.57555784146918	27.656033918191376	30.432281433885365	20.33612680645408
37	19.707067210735776	28.211640933887804	31.735902223565603	20.345389631810818
38	20.603611201843464	28.045885111714547	30.01967944124037	21.33082424520161
39	21.59668358351087	27.59216917494227	29.24322716835429	21.56792007319257
40	21.317498777144547	27.551867759355048	30.523934653204694	20.60669881029571
41	19.445920537958898	27.79627634420659	30.712603780207782	22.045199337626734
42	20.41835469470865	27.612969905567937	31.03566512773761	20.933010271985804
43	21.206507378571672	27.583881383833607	30.062255936739778	21.14735530085494
44	20.26689937484054	28.08797409008991	29.678092442997063	21.967034092072485
45	20.55924714355592	27.762962674063928	29.040582550462084	22.63720763191807
46	21.007112874839727	28.41997325156046	29.224864023348818	21.34804985025099
47	20.018103135872646	27.40918774771964	31.4069906705473	21.16571844586041
48	20.690064238506377	26.830667427193376	30.716991434324132	21.76227689997611
49	20.695751938286833	26.467304664076323	30.814494859131926	22.022448538504914
50	19.727542929945415	27.073450954964795	30.559198391843513	22.63980772324628
51	19.885335972426034	26.602834424559163	29.383144582953474	24.12868502006133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	462.5
2	758.0
3	3314.5
4	5871.0
5	4473.5
6	3076.0
7	2490.0
8	1904.0
9	1845.5
10	1787.0
11	1780.0
12	1773.0
13	1851.5
14	1930.0
15	1919.0
16	1908.0
17	1891.0
18	1874.0
19	2122.5
20	2371.0
21	2777.0
22	3183.0
23	3879.5
24	4576.0
25	5484.0
26	7623.5
27	8855.0
28	10492.0
29	12129.0
30	13886.5
31	15644.0
32	17973.5
33	20303.0
34	22877.5
35	25452.0
36	27494.0
37	29536.0
38	31281.5
39	33027.0
40	35132.0
41	37237.0
42	40619.5
43	44002.0
44	46784.0
45	49566.0
46	52751.0
47	55936.0
48	54993.5
49	54051.0
50	50619.0
51	47187.0
52	42745.5
53	38304.0
54	33524.5
55	28745.0
56	25243.0
57	21741.0
58	19815.0
59	17889.0
60	15980.5
61	14072.0
62	12148.0
63	10224.0
64	8168.5
65	6113.0
66	4696.5
67	3280.0
68	2475.5
69	1671.0
70	1365.0
71	1059.0
72	857.5
73	656.0
74	556.5
75	398.5
76	340.0
77	245.5
78	151.0
79	119.5
80	88.0
81	58.5
82	29.0
83	24.0
84	19.0
85	16.5
86	14.0
87	9.0
88	4.0
89	4.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615363.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.794664227908505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75787099685544	27.87653968491543
2	8.840738039089414	6.6826545142856535
3	3.950169875856455	4.478860323035812
4	2.4621988409234126	3.722319138201836
5	1.7491282988657768	3.305385837358241
6	1.2979498715009041	2.9433347746820195
7	0.9970583120997073	2.6378438885017554
8	0.7522279104714892	2.274416103930492
9	0.5926087488490802	2.0157703813144754
>10	5.518860088934886	40.4512539456914
>50	0.06255547177439233	1.5516943458079782
>100	0.015971609810595977	0.9752165888636853
>500	0.002218279140360552	0.6125285761896565
>1k	4.436558280721104E-4	0.47218189722148174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2816	0.45761607376459096	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	0.13991741459918777	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGCCG	807	0.13114210636648613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCGCAGTCGTATGC	775	0.12594192371007032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06288970900102867	0.0
2	0.0	0.0	0.0	0.269434463885544	0.0
3	0.0	0.0	0.0	0.4059392586164589	0.0
4	0.0	0.0	0.0	0.6043587281003245	0.0
5	0.0	0.0	0.0	1.2235054756298316	0.0
6	0.0	0.0	0.0	1.6089690150366531	0.0
7	0.0	0.0	0.0	1.967456606913318	0.0
8	0.0	0.0	0.0	2.5926160656393056	0.0
9	0.0	0.0	0.0	2.8352370877027058	0.0
10	0.0	0.0	0.0	3.6112018434647517	0.0
11	0.0	0.0	0.0	4.475244692969841	0.0
12	0.0	0.0	0.0	5.2512094487318866	0.0
13	0.0	0.0	0.0	5.542744688907198	0.0
14	0.0	0.0	0.0	5.658773764428475	0.0
15	0.0	0.0	0.0	5.881731595822303	0.0
16	0.0	0.0	0.0	6.323422110201621	0.0
17	0.0	0.0	0.0	6.906655096260256	0.0
18	0.0	0.0	0.0	7.5050011131640995	0.0
19	0.0	0.0	0.0	7.8847769527904665	0.0
20	0.0	0.0	0.0	8.24586463599534	0.0
21	0.0	0.0	0.0	8.709493420956411	0.0
22	0.0	0.0	0.0	9.213098610088679	0.0
23	0.0	0.0	0.0	9.739292092634754	0.0
24	0.0	0.0	0.0	10.132230894610172	0.0
25	0.0	0.0	0.0	10.499656300427553	0.0
26	0.0	0.0	0.0	10.83441805893432	0.0
27	0.0	0.0	0.0	11.167879771776983	0.0
28	0.0	0.0	0.0	11.524904812281532	0.0
29	0.0	0.0	0.0	11.914430994388677	0.0
30	0.0	0.0	0.0	12.343608569250994	0.0
31	0.0	0.0	0.0	12.762548284508494	0.0
32	0.0	0.0	0.0	13.155812097899938	0.0
33	0.0	0.0	0.0	13.540950625890735	0.0
34	0.0	0.0	0.0	13.938602093398531	0.0
35	0.0	0.0	0.0	14.366154611180718	0.0
36	0.0	0.0	0.0	14.778756603825709	0.0
37	0.0	0.0	0.0	15.193796182090896	0.0
38	0.0	0.0	0.0	15.609323277480122	0.0
39	0.0	0.0	0.0	16.01900016738088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCGT	85	0.0	45.000004	21
CGAACGG	20	7.030729E-4	45.0	2
CGACGTT	35	1.2107012E-7	45.0	27
CAACCGG	35	1.2107012E-7	45.0	2
ACGGGCC	50	2.1827873E-11	45.0	5
TGTTGCG	25	3.8886858E-5	45.0	1
TTGCGCG	20	7.030729E-4	45.0	1
ACGTCGG	20	7.030729E-4	45.0	2
TTGCACG	40	6.8066583E-9	45.0	1
CGCGTGG	20	7.030729E-4	45.0	2
TTCGCTT	20	7.030729E-4	45.0	23
GCGTATG	20	7.030729E-4	45.0	1
TCGCGTG	20	7.030729E-4	45.0	1
ATCGATA	20	7.030729E-4	45.0	14
CGAAGTT	20	7.030729E-4	45.0	13
TCACGTA	20	7.030729E-4	45.0	30
GCGCGAC	30	2.1638516E-6	44.999996	9
TACGGGT	55	1.8189894E-12	44.999996	4
AGTTCGG	30	2.1638516E-6	44.999996	2
TATACGG	30	2.1638516E-6	44.999996	2
>>END_MODULE