##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936215.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599856
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.908578058734097	31.0	31.0	34.0	30.0	34.0
2	32.020631618255045	33.0	31.0	34.0	30.0	34.0
3	31.71545837667707	33.0	31.0	34.0	30.0	34.0
4	35.5787639033368	37.0	35.0	37.0	33.0	37.0
5	31.137336294043905	37.0	35.0	37.0	0.0	37.0
6	33.388223173561656	37.0	35.0	37.0	17.0	37.0
7	29.598070203515512	35.0	32.0	37.0	0.0	37.0
8	32.65274165799792	35.0	35.0	37.0	17.0	37.0
9	36.551230628684216	39.0	35.0	39.0	32.0	39.0
10	36.88986356725614	38.0	37.0	39.0	33.0	39.0
11	36.97905664026033	39.0	37.0	39.0	33.0	39.0
12	36.96866748019524	39.0	37.0	39.0	33.0	39.0
13	36.82268244378651	39.0	37.0	39.0	33.0	39.0
14	38.08740764450135	40.0	37.0	41.0	33.0	41.0
15	38.175173708356674	40.0	37.0	41.0	33.0	41.0
16	38.22653270118162	40.0	37.0	41.0	33.0	41.0
17	38.19345809660985	40.0	37.0	41.0	33.0	41.0
18	38.079654116988074	40.0	37.0	41.0	33.0	41.0
19	37.95967198794377	40.0	37.0	41.0	33.0	41.0
20	37.816347590088284	40.0	36.0	41.0	33.0	41.0
21	37.69468005654691	40.0	36.0	41.0	33.0	41.0
22	37.66195053479502	39.0	35.0	41.0	33.0	41.0
23	37.58169460670561	39.0	35.0	41.0	33.0	41.0
24	37.52124676589048	39.0	35.0	41.0	33.0	41.0
25	37.36453248779707	39.0	35.0	41.0	33.0	41.0
26	37.22928502840682	39.0	35.0	41.0	32.0	41.0
27	37.170144167933636	39.0	35.0	41.0	32.0	41.0
28	37.035710237123574	39.0	35.0	41.0	32.0	41.0
29	36.96183417353498	39.0	35.0	41.0	31.0	41.0
30	36.793102011149344	39.0	35.0	41.0	31.0	41.0
31	36.65193313061801	39.0	35.0	41.0	31.0	41.0
32	36.48300592142114	39.0	35.0	41.0	30.0	41.0
33	36.220302872689444	39.0	35.0	40.0	30.0	41.0
34	35.986200021338455	39.0	35.0	40.0	29.0	41.0
35	35.782214398122214	39.0	35.0	40.0	27.0	41.0
36	35.571070390226986	38.0	35.0	40.0	26.0	41.0
37	35.44336807500467	38.0	35.0	40.0	26.0	41.0
38	35.25521791896722	38.0	34.0	40.0	25.0	41.0
39	35.236918527113176	38.0	34.0	40.0	25.0	41.0
40	35.06246999279827	38.0	34.0	40.0	24.0	41.0
41	34.91239397455389	38.0	34.0	40.0	23.0	41.0
42	34.856062121575846	38.0	34.0	40.0	23.0	41.0
43	34.725464111386735	38.0	34.0	40.0	23.0	41.0
44	34.57248239577498	38.0	34.0	40.0	23.0	41.0
45	34.44933450694833	38.0	33.0	40.0	23.0	41.0
46	34.353513176495696	37.0	33.0	40.0	23.0	41.0
47	34.19623376276973	37.0	33.0	40.0	22.0	41.0
48	34.07146215091622	37.0	33.0	40.0	22.0	41.0
49	33.91693506441546	37.0	33.0	40.0	21.0	41.0
50	33.74161465418367	36.0	33.0	40.0	20.0	41.0
51	31.903791910058413	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	27.0
10	26.0
11	23.0
12	23.0
13	16.0
14	28.0
15	56.0
16	114.0
17	170.0
18	326.0
19	590.0
20	1072.0
21	1621.0
22	2550.0
23	3794.0
24	5512.0
25	7500.0
26	9300.0
27	10236.0
28	10825.0
29	11815.0
30	13703.0
31	16616.0
32	21628.0
33	30340.0
34	44043.0
35	52122.0
36	59671.0
37	89847.0
38	107309.0
39	98932.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59672321357126	18.936711477421248	23.571157077698647	14.89540823130885
2	35.49551892454189	23.109879704462404	24.55722706782961	16.837374303166094
3	32.74019097916833	22.747292683577392	28.504007628497508	16.008508708756768
4	27.88085807260409	25.464778213437896	28.257781867648234	18.396581846309783
5	21.48082206396202	38.64310767917634	23.61433410685231	16.261736150009334
6	26.444680056546904	34.35457843215705	26.106098797044623	13.09464271425142
7	70.39956256168148	18.267717585553868	8.368841855378625	2.963877997386039
8	84.66998746365795	3.8752633965485046	9.070176842442185	2.3845722973513643
9	79.03446827238537	6.130971433143954	10.923454962524339	3.9111053319463336
10	42.10243791843376	27.83334666986744	17.413012456322853	12.651202955375956
11	31.09296231095463	25.423268251046917	26.368661812168252	17.115107625830202
12	28.749899975994236	22.797137979781816	29.384052172521407	19.06890987170254
13	24.270491584646983	23.95441572644101	31.390033608065938	20.38505908084607
14	21.43347736790163	24.803452828678882	31.769458003254115	21.99361180016537
15	20.9635312474994	25.763683283988158	32.92256808300659	20.350217385505857
16	24.553392814275426	24.987496999279827	31.141307247072632	19.31780293937212
17	24.322003947614093	25.402930036542102	29.26268971219759	21.012376303646207
18	24.434030834066842	24.590401696407138	30.41513296524499	20.560434504281027
19	24.564895574937985	26.399669253954283	27.819676722413377	21.215758448694352
20	25.806026779760476	25.717005414632844	28.99429196340455	19.48267584220213
21	26.026746419140594	26.26980475314075	29.07081032781201	18.632638499906644
22	26.128937611693477	23.498139553492837	29.12915766450615	21.24376517030754
23	24.401856445546933	25.24939318769838	29.696960603878264	20.651789762876422
24	23.27608626070257	24.605738710623886	31.146808567389506	20.971366461284042
25	23.445293537115575	25.91238563922008	29.719466005174578	20.92285481848977
26	22.439218745832335	26.87811741484623	29.08464698194233	21.598016857379104
27	22.309854365047613	26.355491984743008	29.659284895041477	21.675368755167906
28	21.844075911552103	26.808100610813263	30.04821157077699	21.299611906857645
29	22.771131738283852	25.671661198687683	29.829659118188367	21.727547944840094
30	23.447294017230803	25.58397348697021	29.41689338774639	21.5518391080526
31	24.380851404337044	25.892547544744072	27.917366834706996	21.80923421621189
32	24.384518951214957	25.940058947480725	28.21160411832173	21.463817982982583
33	23.80588007788536	26.531700941559304	28.01922461390734	21.643194366647993
34	22.886326051585716	26.08826118268384	29.521585180443306	21.503827585287137
35	23.81388199834627	26.45318209703662	28.475334080179245	21.257601824437867
36	23.759702328558856	28.284121522498733	26.951801765757118	21.004374383185297
37	23.673514976927795	27.88419220612947	27.23303592862287	21.209256888319864
38	22.62492998319597	27.72265343682484	28.28295457576485	21.369462004214345
39	23.71019044570697	27.102337894427997	27.618128350804195	21.56934330906084
40	24.87980448641007	26.033247979515085	28.55785388493238	20.52909364914246
41	21.67470192846283	26.482189058707423	28.783741431276837	23.059367581552905
42	22.34386252700648	26.56804299698594	27.828345469579364	23.25974900642821
43	22.272011949534555	26.86444746739217	28.18876530367288	22.67477527940039
44	22.376870448907738	26.31481555573338	28.087741057853883	23.220572937505
45	22.563581926328986	26.632725187378302	27.572617428182767	23.231075458109945
46	22.87415646421808	26.87961777493265	27.258208636739482	22.988017124109785
47	22.18249046437812	26.076425008668746	29.448901069590033	22.2921834573631
48	22.344696060387825	25.919887439652182	29.737303619535354	21.998112880424635
49	22.46205756048118	26.592382171721212	28.618034995065482	22.32752527273212
50	21.15491051185618	26.342155450641485	29.727134512282948	22.775799525219387
51	21.638359873036194	26.179116321250433	28.48400282734523	23.698520978368144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	228.0
1	630.0
2	1032.0
3	5855.5
4	10679.0
5	7929.5
6	5180.0
7	4066.0
8	2952.0
9	2835.5
10	2719.0
11	2632.5
12	2546.0
13	2551.5
14	2557.0
15	2456.0
16	2355.0
17	2377.0
18	2399.0
19	2358.5
20	2318.0
21	2400.0
22	2482.0
23	2686.0
24	2890.0
25	3384.0
26	4629.5
27	5381.0
28	6926.5
29	8472.0
30	9681.0
31	10890.0
32	12294.5
33	13699.0
34	15666.0
35	17633.0
36	18833.5
37	20034.0
38	21257.0
39	22480.0
40	24328.5
41	26177.0
42	28490.0
43	30803.0
44	32732.0
45	34661.0
46	37097.5
47	39534.0
48	40726.0
49	41918.0
50	41527.0
51	41136.0
52	39380.5
53	37625.0
54	36500.5
55	35376.0
56	34292.0
57	33208.0
58	32018.5
59	30829.0
60	28805.5
61	26782.0
62	24439.0
63	22096.0
64	19616.5
65	17137.0
66	14993.0
67	12849.0
68	11098.5
69	9348.0
70	7928.0
71	6508.0
72	5331.0
73	4154.0
74	3604.0
75	2427.0
76	1800.0
77	1383.0
78	966.0
79	760.5
80	555.0
81	404.0
82	253.0
83	186.0
84	119.0
85	103.0
86	87.0
87	63.5
88	40.0
89	28.0
90	16.0
91	11.5
92	7.0
93	8.5
94	10.0
95	6.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	599856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.05906159533978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33684796863584	28.254094004196762
2	8.55545758886166	6.683362898793295
3	4.29951307293522	5.03804837837236
4	2.747306897582657	4.292289173359313
5	1.9070957704802265	3.724468558369956
6	1.4029253753458581	3.287816918957943
7	1.1781204174111832	3.221139456527357
8	0.9342837964399023	2.9193798682139227
9	0.7820928961861561	2.749303314487147
>10	5.809859420255456	36.55779293036437
>50	0.030555037289439323	0.7811050858872097
>100	0.013727625440792436	0.888169755122913
>500	8.856532542446733E-4	0.23350129881517676
>1k	8.856532542446733E-4	0.45818143745289125
>5k	4.4282662712233665E-4	0.9113469210793824
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5269	0.8783774772612094	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1570	0.26172948174228483	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1079	0.17987650369421995	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.11436077992051427	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	664	0.11069323304259689	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.036342055426635725	0.0
2	0.0	0.0	0.0	0.11969539356112134	0.0
3	0.0	0.0	0.0	0.2078832253074071	0.0
4	0.0	0.0	0.0	0.28940279000293406	0.0
5	0.0	0.0	0.0	0.5671361126670401	0.0
6	0.0	0.0	0.0	0.9347243338401217	0.0
7	0.0	0.0	0.0	1.1892854285028407	0.0
8	0.0	0.0	0.0	1.610386492758262	0.0
9	0.0	0.0	0.0	1.8436091328585527	0.0
10	0.0	0.0	0.0	2.2216998746365793	0.0
11	0.0	0.0	0.0	2.680643354405057	0.0
12	0.0	0.0	0.0	3.0092222133311997	0.0
13	0.0	0.0	0.0	3.202101837774399	0.0
14	0.0	0.0	0.0	3.3076271638526578	0.0
15	0.0	0.0	0.0	3.4383251980475316	0.0
16	0.0	0.0	0.0	3.659378250780187	0.0
17	0.0	0.0	0.0	3.9227747992851616	0.0
18	0.0	0.0	0.0	4.1946733882798535	0.0
19	0.0	0.0	0.0	4.38321863914006	0.0
20	1.6670667626897121E-4	0.0	0.0	4.586434077511936	0.0
21	1.6670667626897121E-4	0.0	0.0	4.825491451281641	0.0
22	1.6670667626897121E-4	0.0	0.0	5.089888239844229	0.0
23	1.6670667626897121E-4	0.0	0.0	5.3622869488677285	0.0
24	1.6670667626897121E-4	0.0	0.0	5.58067269478008	0.0
25	1.6670667626897121E-4	0.0	0.0	5.7918900536128675	0.0
26	1.6670667626897121E-4	0.0	0.0	5.990104291696674	0.0
27	1.6670667626897121E-4	0.0	0.0	6.19165266330586	0.0
28	1.6670667626897121E-4	0.0	0.0	6.402536608786109	0.0
29	1.6670667626897121E-4	0.0	0.0	6.623089541489958	0.0
30	1.6670667626897121E-4	0.0	0.0	6.93216371929263	0.0
31	1.6670667626897121E-4	0.0	0.0	7.195060147768798	0.0
32	1.6670667626897121E-4	0.0	0.0	7.442952975380758	0.0
33	1.6670667626897121E-4	0.0	0.0	7.703182097036622	0.0
34	1.6670667626897121E-4	0.0	0.0	7.949574564562162	0.0
35	1.6670667626897121E-4	0.0	0.0	8.216972073297592	0.0
36	1.6670667626897121E-4	0.0	0.0	8.474033768104345	0.0
37	1.6670667626897121E-4	0.0	0.0	8.72442719586034	0.0
38	1.6670667626897121E-4	0.0	0.0	9.012162919100584	0.0
39	1.6670667626897121E-4	0.0	0.0	9.378417486863514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	20	7.0305855E-4	45.000004	9
TATTGCG	20	7.0305855E-4	45.000004	1
ACTACGG	20	7.0305855E-4	45.000004	2
AATCGGC	20	7.0305855E-4	45.000004	12
CTTAACG	25	3.888568E-5	45.0	1
GCGTTAG	25	3.888568E-5	45.0	1
GGACGTA	25	3.888568E-5	45.0	8
TATACGG	35	1.2106284E-7	45.0	2
ACGGGTA	30	2.1637607E-6	44.999996	5
CGTCATA	30	2.1637607E-6	44.999996	38
CGTTATT	395	0.0	44.430378	1
CGTTTTT	1810	0.0	42.140884	1
GCTACGA	145	0.0	41.89655	10
CACGACC	195	0.0	40.384617	27
TATAGCG	40	3.4545337E-7	39.375004	1
ACGACCA	200	0.0	39.375	28
CGAATAT	155	0.0	39.193546	14
GACCTAT	155	0.0	39.193546	33
CACGTGA	190	0.0	39.078945	43
CGTTTTA	225	0.0	39.0	1
>>END_MODULE