##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936214.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635795
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833636628158448	31.0	31.0	34.0	30.0	34.0
2	31.936186978507223	33.0	31.0	34.0	30.0	34.0
3	31.321981141720208	33.0	31.0	34.0	28.0	34.0
4	35.368477260752286	37.0	35.0	37.0	33.0	37.0
5	31.138079097822413	37.0	35.0	37.0	0.0	37.0
6	33.39349318569665	37.0	35.0	37.0	17.0	37.0
7	29.53818762336917	35.0	32.0	37.0	0.0	37.0
8	32.65263017167483	35.0	35.0	37.0	17.0	37.0
9	36.60203367437617	39.0	35.0	39.0	32.0	39.0
10	36.96606610621348	39.0	37.0	39.0	34.0	39.0
11	37.04439953129546	39.0	37.0	39.0	34.0	39.0
12	37.08549768400192	39.0	37.0	39.0	34.0	39.0
13	36.99078161986175	39.0	37.0	39.0	33.0	39.0
14	38.31062685299507	40.0	38.0	41.0	34.0	41.0
15	38.3923670365448	40.0	38.0	41.0	34.0	41.0
16	38.423137961135275	40.0	38.0	41.0	34.0	41.0
17	38.38572810418452	40.0	38.0	41.0	34.0	41.0
18	38.20922152580627	40.0	37.0	41.0	34.0	41.0
19	38.014150787596634	40.0	37.0	41.0	34.0	41.0
20	37.78257614482656	39.0	35.0	41.0	34.0	41.0
21	37.65564844014187	39.0	35.0	41.0	33.0	41.0
22	37.575504683113266	39.0	35.0	41.0	33.0	41.0
23	37.49551191814972	39.0	35.0	41.0	33.0	41.0
24	37.4097421338639	39.0	35.0	41.0	33.0	41.0
25	37.27068473328667	39.0	35.0	41.0	33.0	41.0
26	37.11992072916585	39.0	35.0	41.0	33.0	41.0
27	37.057063990751736	39.0	35.0	41.0	32.0	41.0
28	36.939150197783874	39.0	35.0	41.0	32.0	41.0
29	36.87231418932203	39.0	35.0	41.0	32.0	41.0
30	36.676098427952404	39.0	35.0	41.0	31.0	41.0
31	36.47449885576326	39.0	35.0	40.0	31.0	41.0
32	36.23774487059508	39.0	35.0	40.0	30.0	41.0
33	35.87415125944683	38.0	35.0	40.0	29.0	41.0
34	35.53182708262884	38.0	35.0	40.0	25.0	41.0
35	35.20289244174616	38.0	34.0	40.0	24.0	41.0
36	34.96654582058682	38.0	34.0	40.0	23.0	41.0
37	34.787978829654215	38.0	34.0	40.0	22.0	41.0
38	34.61448108273893	38.0	34.0	40.0	21.0	41.0
39	34.556923222107756	38.0	34.0	40.0	21.0	41.0
40	34.402552709599796	38.0	34.0	40.0	20.0	41.0
41	34.28370308039541	38.0	33.0	40.0	19.0	41.0
42	34.19694712918472	38.0	33.0	40.0	18.0	41.0
43	34.0588664585283	38.0	33.0	40.0	18.0	41.0
44	33.826573030615215	38.0	33.0	40.0	18.0	41.0
45	33.74603449224986	37.0	33.0	40.0	18.0	41.0
46	33.681848709096485	37.0	33.0	40.0	18.0	41.0
47	33.59887227801414	37.0	33.0	40.0	18.0	41.0
48	33.491946303446866	37.0	33.0	40.0	18.0	41.0
49	33.37607562185925	37.0	32.0	40.0	17.0	41.0
50	33.2196415511289	37.0	32.0	40.0	17.0	41.0
51	31.561712501671135	35.0	29.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	31.0
10	26.0
11	23.0
12	19.0
13	9.0
14	22.0
15	42.0
16	99.0
17	168.0
18	367.0
19	700.0
20	1189.0
21	1902.0
22	3134.0
23	4787.0
24	7377.0
25	10963.0
26	13694.0
27	14297.0
28	13730.0
29	13562.0
30	14783.0
31	17201.0
32	21730.0
33	30161.0
34	43611.0
35	52436.0
36	59694.0
37	92223.0
38	111846.0
39	105938.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52265274184289	18.871963447337585	20.074709615520725	20.530674195298797
2	40.3158250694013	22.26708294340157	21.723511509212877	15.693580477984256
3	30.86671018174097	22.74365164872325	31.00889437633199	15.380743793203786
4	26.60794753025739	24.74366737706336	32.07779237018221	16.570592722497032
5	20.224128846562177	37.87635951839823	26.584984153697338	15.314527481342258
6	25.416368483552088	32.56977484881133	29.78271298138551	12.231143686251071
7	67.30188189589413	18.012095093544303	12.29562988070054	2.39039312986104
8	81.9245196958139	2.8399090901941664	13.418633364527874	1.816937849464057
9	76.23259069354116	5.338355916608341	15.010498667023175	3.4185547228273263
10	39.07045509952107	28.80676947758318	20.80906581523919	11.313709607656557
11	26.625248704377984	24.135924315227392	32.890161136844426	16.3486658435502
12	24.567667251236642	22.484134036914412	35.07765867929128	17.870540032557663
13	22.414142923426574	22.15666999583199	36.67833185224797	18.75085522849346
14	20.393994919746145	23.540292075275836	36.49509668997082	19.570616315007193
15	20.237340652254264	24.330483882383472	36.600004718502035	18.83217074686023
16	24.162033359809374	23.70135027799841	34.63553503880968	17.501081323382536
17	24.309722473438768	23.11468319190934	34.042733900077856	18.532860434574037
18	23.673825682806566	23.429879127706258	34.55673605486045	18.339559134626725
19	23.165013880260148	24.653701271636297	33.40951092726429	18.771773920839262
20	24.290848465307214	24.727939036953735	33.312467068787896	17.668745428951155
21	25.17541031307261	23.511351929474124	33.96975440196919	17.34348335548408
22	24.334887817614167	22.13685228729386	34.38765639868196	19.140603496410005
23	22.65620207771373	23.31946618013668	34.74799267059351	19.276339071556084
24	21.66814775202699	23.465425176354014	35.464733129389195	19.401693942229805
25	21.90643210468783	24.722591401316464	34.218734025904574	19.15224246809113
26	21.64502709206584	25.36682421220676	33.54870673723448	19.43944195849291
27	21.11718399798677	24.720232150300017	34.45686109516432	19.705722756548887
28	20.455492729574782	25.431624973458426	34.12184745082928	19.991034846137513
29	21.664530233801777	23.993897404037465	34.48344198994959	19.858130372211168
30	21.970918299137303	24.2829842952524	34.10108604188457	19.64501136372573
31	22.576616676759016	25.048325324986827	32.860277290636134	19.51478070761802
32	22.488223405342918	24.811771089738045	33.16273327094425	19.537272233974786
33	22.278092781478307	25.234077021681518	32.49459338308732	19.99323681375286
34	22.15415346141445	25.218820531775176	32.70378030654535	19.92324570026502
35	22.188441242853436	26.144276063825604	32.337624548793244	19.329658144527716
36	22.98083501757642	26.922357049048827	30.369065500672388	19.727742432702364
37	22.934750981055213	26.642078028295284	30.809301740340832	19.613869250308667
38	22.694736510982313	26.61235146548809	30.822670829434017	19.87024119409558
39	22.96699407827995	26.626821538388945	29.822033831659578	20.584150551671527
40	23.523777318160725	26.31964705604794	30.18394293758208	19.97263268820925
41	22.094385768997867	26.150881966671648	30.544279209493624	21.210453054836858
42	22.151794210398005	26.385076950903986	30.511564262065605	20.951564576632407
43	22.088251716355114	25.71693706304705	30.84358952177982	21.351221698818016
44	21.93002461485227	26.400333440810325	30.749219481122058	20.920422463215342
45	21.80168135955772	26.008383205278427	30.422541857045115	21.76739357811873
46	22.232008744957103	26.246038424334888	30.61033823795406	20.911614592753956
47	21.794918173310577	26.197752420198334	31.373949150276424	20.63338025621466
48	21.730589262262207	26.030717448234103	31.389048356781668	20.849644932722025
49	22.204955999968544	25.953019448092547	31.146045502087937	20.695979049850973
50	21.427032298146415	26.381144865876582	31.601852798464915	20.589970037512092
51	21.708255019306538	25.805959468067535	30.99851367185964	21.487271840766283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	221.0
1	608.0
2	995.0
3	10805.5
4	20616.0
5	15511.5
6	10407.0
7	8127.5
8	5848.0
9	5639.5
10	5431.0
11	5291.0
12	5151.0
13	4970.0
14	4789.0
15	4557.0
16	4325.0
17	4065.0
18	3805.0
19	3735.5
20	3666.0
21	3639.0
22	3612.0
23	3882.0
24	4152.0
25	4806.5
26	6432.0
27	7403.0
28	8671.0
29	9939.0
30	11197.0
31	12455.0
32	13734.5
33	15014.0
34	17144.5
35	19275.0
36	20374.0
37	21473.0
38	23359.5
39	25246.0
40	26896.0
41	28546.0
42	30136.0
43	31726.0
44	33641.0
45	35556.0
46	37789.0
47	40022.0
48	40799.0
49	41576.0
50	40991.0
51	40406.0
52	38786.0
53	37166.0
54	35455.5
55	33745.0
56	31811.5
57	29878.0
58	29005.0
59	28132.0
60	26420.5
61	24709.0
62	22782.0
63	20855.0
64	18603.0
65	16351.0
66	14377.5
67	12404.0
68	10657.0
69	8910.0
70	7409.0
71	5908.0
72	4956.5
73	4005.0
74	3403.0
75	2290.0
76	1779.0
77	1348.5
78	918.0
79	694.0
80	470.0
81	401.0
82	332.0
83	248.0
84	164.0
85	117.0
86	70.0
87	53.0
88	36.0
89	31.5
90	27.0
91	18.0
92	9.0
93	8.5
94	8.0
95	4.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	635795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.57646820510546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.26120141708691	30.617350096041985
2	9.9130773353772	8.639537986396322
3	5.17228086470937	6.761691979466495
4	3.319594868698044	5.786248809986063
5	2.2555339733046615	4.914410223662289
6	1.756358796831632	4.592154796013454
7	1.3757517225958926	4.196528083847328
8	1.122317102246198	3.9125292417662028
9	0.9046318473246294	3.5478594839040785
>10	3.8939602072020336	23.454952975877667
>50	0.01748114203827846	0.5304811687788213
>100	0.006322965902186715	0.4805611350028591
>500	0.0	0.0
>1k	0.0011158175121505813	0.9872168206753424
>5k	3.7193917071686047E-4	1.5784771985810475
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9739	1.5317830432765278	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2823	0.444011041294757	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1921	0.30214141350592566	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1347	0.21186074127666937	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.572834010962653E-4	0.0	0.0	0.016200190312915325	0.0
2	1.572834010962653E-4	0.0	0.0	0.060554109422062144	0.0
3	1.572834010962653E-4	0.0	0.0	0.13589285854717323	0.0
4	1.572834010962653E-4	0.0	0.0	0.1967615347714279	0.0
5	1.572834010962653E-4	0.0	0.0	0.39541047035601096	0.0
6	1.572834010962653E-4	0.0	0.0	0.7288512806800934	0.0
7	1.572834010962653E-4	0.0	0.0	0.9506208762258275	0.0
8	1.572834010962653E-4	0.0	0.0	1.23436013180349	0.0
9	1.572834010962653E-4	0.0	0.0	1.4404013872395978	0.0
10	1.572834010962653E-4	0.0	0.0	1.6863926265541567	0.0
11	1.572834010962653E-4	0.0	0.0	1.996083643312703	0.0
12	1.572834010962653E-4	0.0	0.0	2.2093599351992386	0.0
13	1.572834010962653E-4	0.0	0.0	2.3521732633946475	0.0
14	1.572834010962653E-4	0.0	0.0	2.4621143607609373	0.0
15	1.572834010962653E-4	0.0	0.0	2.5726845917316115	0.0
16	1.572834010962653E-4	0.0	0.0	2.7244630737895075	0.0
17	1.572834010962653E-4	0.0	0.0	2.881903758286869	0.0
18	1.572834010962653E-4	0.0	0.0	3.0417036938006747	0.0
19	1.572834010962653E-4	0.0	0.0	3.1706760826996123	0.0
20	1.572834010962653E-4	0.0	0.0	3.303265989823764	0.0
21	1.572834010962653E-4	0.0	0.0	3.4583474233046814	0.0
22	1.572834010962653E-4	0.0	0.0	3.6046209863242082	0.0
23	1.572834010962653E-4	0.0	0.0	3.7689821404698054	0.0
24	1.572834010962653E-4	0.0	0.0	3.91619940389591	0.0
25	1.572834010962653E-4	0.0	0.0	4.053193246250757	0.0
26	1.572834010962653E-4	0.0	0.0	4.1882996877924485	0.0
27	1.572834010962653E-4	0.0	0.0	4.330641165784569	0.0
28	1.572834010962653E-4	0.0	0.0	4.493272202518107	0.0
29	1.572834010962653E-4	0.0	0.0	4.640804032746404	0.0
30	1.572834010962653E-4	0.0	0.0	4.849047255797859	0.0
31	1.572834010962653E-4	0.0	0.0	5.039517454525437	0.0
32	1.572834010962653E-4	0.0	0.0	5.227471118835473	0.0
33	1.572834010962653E-4	0.0	0.0	5.405987779079735	0.0
34	1.572834010962653E-4	0.0	0.0	5.585133572928381	0.0
35	1.572834010962653E-4	0.0	0.0	5.7729299538373215	0.0
36	1.572834010962653E-4	0.0	0.0	5.947986379257465	0.0
37	1.572834010962653E-4	0.0	0.0	6.130749691331325	0.0
38	1.572834010962653E-4	0.0	0.0	6.316029537822726	0.0
39	1.572834010962653E-4	0.0	0.0	6.554785740686857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	30	2.1639644E-6	45.000004	1
GTAAGCG	20	7.030906E-4	45.0	1
GCTAACG	25	3.888832E-5	45.0	1
CGTTATT	800	0.0	44.4375	1
CGTTTTT	3975	0.0	43.132076	1
CGTTTTA	515	0.0	41.504852	1
CTACGAA	55	6.002665E-11	40.909092	11
TACGAAT	55	6.002665E-11	40.909092	12
AGGCGAT	210	0.0	40.714287	7
CGGTTTT	875	0.0	40.62857	1
ACTACGG	50	1.0804797E-9	40.5	2
ATAGCGG	45	1.925946E-8	40.000004	2
CCATACG	30	1.13934795E-4	37.500004	2
CGAATAT	60	1.546141E-10	37.500004	14
TTAACGG	30	1.13934795E-4	37.500004	2
GCTACGA	60	1.546141E-10	37.500004	10
TCCGAAC	55	2.744855E-9	36.81818	17
GTTACGG	55	2.744855E-9	36.81818	2
GACGGGT	55	2.744855E-9	36.81818	4
AACGGGA	295	0.0	36.61017	4
>>END_MODULE