FastQCFastQC Report
Sat 14 Jan 2017
SRR2936213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669773
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.2869628963842974No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC18460.27561576832747814No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG17930.2677026395510121No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC11870.17722422372953225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGG650.045.0000042
TAGCACG351.2109194E-745.01
GTATGCG351.2109194E-745.01
CGTTTAC207.031176E-445.033
CGTTTTT7900.039.5886081
TCACGCC356.244385E-638.57142632
ACCGATC356.244385E-638.57142610
CACCGCT356.244385E-638.57142640
TATACGG356.244385E-638.5714262
ACGGGAT3600.038.1255
TCACGAC301.1394133E-437.49999625
GTCCGAA301.1394133E-437.4999969
ACGACAA301.1394133E-437.49999632
TACGGGA2900.037.241384
GCTTGCG552.744855E-936.8181841
TTTACGG800.036.56252
ACGGGAG3100.036.290325
ATTTACG250.002106482236.01
TAATACG250.002106482236.04
CGAACTA250.002106482236.039
Produced by FastQC (version 0.11.5)