##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936213.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669773
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93346402437841	31.0	31.0	34.0	30.0	34.0
2	32.07390712972903	33.0	31.0	34.0	30.0	34.0
3	31.955665576247476	33.0	31.0	34.0	30.0	34.0
4	35.70385488814867	37.0	35.0	37.0	35.0	37.0
5	31.191318551210635	37.0	35.0	37.0	0.0	37.0
6	33.409295388138965	37.0	35.0	37.0	17.0	37.0
7	29.5739944727542	35.0	32.0	37.0	0.0	37.0
8	32.62309618333376	35.0	33.0	37.0	17.0	37.0
9	36.5341989002244	39.0	35.0	39.0	32.0	39.0
10	36.81849223542902	38.0	37.0	39.0	33.0	39.0
11	36.91253305224307	39.0	37.0	39.0	33.0	39.0
12	36.95730045851356	39.0	37.0	39.0	33.0	39.0
13	36.81438935281058	39.0	37.0	39.0	33.0	39.0
14	38.047856512579635	40.0	37.0	41.0	33.0	41.0
15	38.13786461980402	40.0	37.0	41.0	33.0	41.0
16	38.18125842636236	40.0	37.0	41.0	33.0	41.0
17	38.147860543796185	40.0	37.0	41.0	33.0	41.0
18	38.0395581786665	40.0	37.0	41.0	33.0	41.0
19	37.963829536275725	40.0	37.0	41.0	33.0	41.0
20	37.91179250283305	40.0	36.0	41.0	33.0	41.0
21	37.82746691789606	40.0	36.0	41.0	33.0	41.0
22	37.82665768850043	40.0	36.0	41.0	33.0	41.0
23	37.79904982732956	40.0	36.0	41.0	33.0	41.0
24	37.72571005400337	40.0	36.0	41.0	33.0	41.0
25	37.61310772455742	39.0	36.0	41.0	33.0	41.0
26	37.452938532905925	39.0	36.0	41.0	33.0	41.0
27	37.44412509910074	39.0	36.0	41.0	33.0	41.0
28	37.347885029704095	39.0	36.0	41.0	32.0	41.0
29	37.302065028002026	39.0	36.0	41.0	32.0	41.0
30	37.161280015766536	39.0	35.0	41.0	32.0	41.0
31	37.06790957533373	39.0	35.0	41.0	31.0	41.0
32	36.95798875141279	39.0	35.0	41.0	31.0	41.0
33	36.82376417084594	39.0	35.0	41.0	31.0	41.0
34	36.68522618857434	39.0	35.0	41.0	30.0	41.0
35	36.57593692191235	39.0	35.0	41.0	30.0	41.0
36	36.472719861803924	39.0	35.0	41.0	30.0	41.0
37	36.36597921982523	39.0	35.0	40.0	30.0	41.0
38	36.25230488538654	39.0	35.0	40.0	30.0	41.0
39	36.21668834067662	39.0	35.0	40.0	30.0	41.0
40	36.09831689243968	39.0	35.0	40.0	29.0	41.0
41	36.00127804494956	39.0	35.0	40.0	29.0	41.0
42	35.95381718880875	39.0	35.0	40.0	29.0	41.0
43	35.823399868313594	39.0	35.0	40.0	28.0	41.0
44	35.67689650075474	38.0	35.0	40.0	27.0	41.0
45	35.547573580899794	38.0	35.0	40.0	27.0	41.0
46	35.42798530248308	38.0	34.0	40.0	26.0	41.0
47	35.27778814613309	38.0	34.0	40.0	26.0	41.0
48	35.17373348880889	38.0	34.0	40.0	26.0	41.0
49	35.01286853904233	38.0	34.0	40.0	24.0	41.0
50	34.88129709618035	38.0	34.0	40.0	24.0	41.0
51	32.84321404416123	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	27.0
11	21.0
12	12.0
13	14.0
14	20.0
15	28.0
16	79.0
17	161.0
18	285.0
19	521.0
20	894.0
21	1315.0
22	2027.0
23	2977.0
24	4193.0
25	5893.0
26	7034.0
27	8178.0
28	9476.0
29	11197.0
30	13636.0
31	17844.0
32	23134.0
33	32529.0
34	46794.0
35	56086.0
36	65554.0
37	103710.0
38	129581.0
39	126518.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.792598387811985	19.96273364259234	24.333020291949662	11.911647677646009
2	33.42565316905877	24.230448226488676	25.230936451603753	17.1129621528488
3	31.900658879948878	24.915755039393943	26.565866345761926	16.617719734895257
4	28.30645606795138	25.554329601223102	27.957979793153797	18.18123453767172
5	22.231562036690043	39.225677953575314	21.847551334556634	16.69520867517801
6	26.701733273810678	35.4494731797191	24.72569064444222	13.123102902028
7	71.7892479989489	18.370104498091145	6.712871375824346	3.127776127135612
8	85.75039602969962	4.187388861599377	7.145405980832312	2.916809127868696
9	80.07235287179387	5.886024070841913	8.63829984188673	5.403323215477482
10	40.22527035279117	29.30530194558455	16.543664793892855	13.925762907731427
11	28.134009582351034	27.12127840327992	26.725323355823537	18.019388658545505
12	26.624692246477537	24.277628390514398	29.907147645545578	19.190531717462484
13	23.658314085518526	25.25064462138665	31.542627128892924	19.5484141642019
14	20.50411109435585	27.72073523417636	30.949888992240655	20.82526467922714
15	20.32524452314441	27.360463918372346	33.71082441364462	18.603467144838625
16	23.794479622200356	27.057077547168966	29.971199197339992	19.177243633290683
17	23.891378123632933	25.624204021362463	28.563856709661334	21.920561145343274
18	23.1321656740418	26.18349799111042	30.43135510090732	20.252981233940453
19	23.34820902007098	27.674600200366395	28.374538836292295	20.60265194327033
20	24.41678001352697	28.214633913281066	28.931742545608735	18.43684352758323
21	24.412300884030856	27.19458682269963	29.85937026425371	18.5337420290158
22	23.10753046181318	24.66507309192816	30.110052211719495	22.117344234539164
23	22.25739168345096	26.642011547195843	29.806217927566504	21.294378841786695
24	22.081361894253725	26.024936806948023	30.45151118363983	21.442190115158418
25	22.160493182018385	28.071600378038532	28.823944829068953	20.943961610874133
26	20.92723952742198	28.210453392418028	28.480096988084025	22.382210092075972
27	22.145114837415065	27.387338695349023	29.159133019694732	21.308413447541184
28	19.98244181237524	27.16592039392451	31.01782245626503	21.83381533743522
29	22.095695108641287	25.989551683928735	30.106170299489527	21.80858290794045
30	22.68917976687624	26.54078322058369	29.422804442699242	21.347232569840827
31	22.542413623720275	26.34773273930123	29.558223457798388	21.55163017918011
32	23.339101456762215	27.352998702545488	28.106089675158596	21.201810165533697
33	22.635728821555958	26.449558283179524	27.976792137037478	22.93792075822704
34	20.94187135044261	25.62748871632628	31.4463258447265	21.984314088504615
35	21.13044270222896	26.25232728103402	29.432061310324542	23.18516870641247
36	22.614228999974618	26.311750399015786	28.701515289508535	22.37250531150106
37	21.621803207952546	27.37300548096146	29.60331336139259	21.401877949693404
38	20.42736867565578	27.66997176655374	28.307202589534064	23.59545696825641
39	21.25914302308394	26.947637483147275	29.21064300890003	22.582576484868756
40	22.244551512228767	25.045649794781216	29.784120888719013	22.925677804271
41	20.23879732386943	25.830094673867116	29.569122672905596	24.361985329357857
42	20.855722759800706	25.41114676166403	30.172610720348537	23.56051975818673
43	21.48728001875262	25.61300619762218	29.093737729051483	23.805976054573712
44	21.313340489986906	26.026579154429935	28.780795881589732	23.87928447399343
45	21.12193235618635	25.880410228540118	28.28435902910389	24.713298386169644
46	22.39997730574389	26.308913617001583	28.279581290974704	23.011527786279828
47	20.19818654977134	25.86473327530372	30.737428949808365	23.19965122511657
48	20.99099247058332	25.872497099763653	29.484765734062137	23.651744695590896
49	21.09625201374197	25.155687076069057	30.50944125845622	23.23861965173275
50	20.343011736812322	25.823226675306408	30.02524735992642	23.808514227954845
51	20.607578985716057	25.246762709156684	28.70808467943617	25.43757362569109
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	242.0
1	591.5
2	941.0
3	2519.0
4	4097.0
5	3170.5
6	2244.0
7	1768.5
8	1293.0
9	1328.0
10	1363.0
11	1421.0
12	1479.0
13	1440.5
14	1402.0
15	1388.0
16	1374.0
17	1532.0
18	1690.0
19	1864.5
20	2039.0
21	2168.5
22	2298.0
23	2785.5
24	3273.0
25	4283.0
26	6403.5
27	7514.0
28	9243.5
29	10973.0
30	12621.5
31	14270.0
32	15602.0
33	16934.0
34	20123.0
35	23312.0
36	25628.5
37	27945.0
38	29454.5
39	30964.0
40	32948.5
41	34933.0
42	38513.5
43	42094.0
44	45971.0
45	49848.0
46	55177.0
47	60506.0
48	58808.5
49	57111.0
50	54539.5
51	51968.0
52	47792.0
53	43616.0
54	39672.5
55	35729.0
56	32723.0
57	29717.0
58	28131.5
59	26546.0
60	24228.5
61	21911.0
62	19263.0
63	16615.0
64	14492.0
65	12369.0
66	10719.5
67	9070.0
68	7900.5
69	6731.0
70	5274.5
71	3818.0
72	3158.0
73	2498.0
74	2107.5
75	1289.0
76	861.0
77	678.0
78	495.0
79	365.5
80	236.0
81	205.5
82	175.0
83	159.5
84	144.0
85	99.5
86	55.0
87	43.5
88	32.0
89	25.5
90	19.0
91	15.0
92	11.0
93	7.0
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	669773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.84627510715184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48099895225197	27.07501102543012
2	8.409139098943028	6.196909053079234
3	3.867948257166695	4.275584567513776
4	2.502400034719688	3.688164804297118
5	1.735238153893482	3.1968531197392758
6	1.3647194624916534	3.0170897255431104
7	0.9716245529826824	2.5060521906044335
8	0.7778456291052562	2.292857123272629
9	0.6531366747145798	2.165908823919337
>10	6.182470267964423	42.25290984952319
>50	0.03631927720195618	0.8620691439020299
>100	0.015625735509097426	1.2394189025222073
>500	8.44634351843104E-4	0.1811278848130382
>1k	0.001689268703686208	1.0500437858405343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1922	0.2869628963842974	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	1846	0.27561576832747814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	1793	0.2677026395510121	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	1187	0.17722422372953225	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.493043165370954E-4	0.0	0.0	0.08092293956310571	0.0
2	1.493043165370954E-4	0.0	0.0	0.37564966040733205	0.0
3	1.493043165370954E-4	0.0	0.0	0.5156971093191275	0.0
4	1.493043165370954E-4	0.0	0.0	0.7459243654193286	0.0
5	1.493043165370954E-4	0.0	0.0	1.6339864401819721	0.0
6	1.493043165370954E-4	0.0	0.0	2.0777188689302197	0.0
7	1.493043165370954E-4	0.0	0.0	2.528916513505322	0.0
8	1.493043165370954E-4	0.0	0.0	3.186303419218153	0.0
9	1.493043165370954E-4	0.0	0.0	3.3998085918661993	0.0
10	1.493043165370954E-4	0.0	0.0	4.320717616267004	0.0
11	1.493043165370954E-4	0.0	0.0	5.057982331327181	0.0
12	1.493043165370954E-4	0.0	0.0	5.839142515449264	0.0
13	1.493043165370954E-4	0.0	0.0	6.0719079449305955	0.0
14	1.493043165370954E-4	0.0	0.0	6.180900096002675	0.0
15	1.493043165370954E-4	0.0	0.0	6.38007205426316	0.0
16	1.493043165370954E-4	0.0	0.0	6.782447187330633	0.0
17	1.493043165370954E-4	0.0	0.0	7.278884039816475	0.0
18	1.493043165370954E-4	0.0	0.0	7.796223496617511	0.0
19	1.493043165370954E-4	0.0	0.0	8.12006455918647	0.0
20	1.493043165370954E-4	0.0	0.0	8.42748214693635	0.0
21	1.493043165370954E-4	0.0	0.0	8.833888496550324	0.0
22	1.493043165370954E-4	0.0	0.0	9.267318927457511	0.0
23	1.493043165370954E-4	0.0	0.0	9.734342829585547	0.0
24	1.493043165370954E-4	0.0	0.0	10.098943970569133	0.0
25	1.493043165370954E-4	0.0	0.0	10.432041900763393	0.0
26	1.493043165370954E-4	0.0	0.0	10.726320708658008	0.0
27	1.493043165370954E-4	0.0	0.0	11.041054207918204	0.0
28	1.493043165370954E-4	0.0	0.0	11.35892309782568	0.0
29	1.493043165370954E-4	0.0	0.0	11.700083461112945	0.0
30	1.493043165370954E-4	0.0	0.0	12.118434156049885	0.0
31	1.493043165370954E-4	0.0	0.0	12.463177822934039	0.0
32	1.493043165370954E-4	0.0	0.0	12.809265228667027	0.0
33	1.493043165370954E-4	0.0	0.0	13.146842288357398	0.0
34	1.493043165370954E-4	0.0	0.0	13.48038813150127	0.0
35	1.493043165370954E-4	0.0	0.0	13.841405968887967	0.0
36	1.493043165370954E-4	0.0	0.0	14.174205290449152	0.0
37	1.493043165370954E-4	0.0	0.0	14.516410783952175	0.0
38	1.493043165370954E-4	0.0	0.0	14.876084882490037	0.0
39	1.493043165370954E-4	0.0	0.0	15.295779316275812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACGG	65	0.0	45.000004	2
TAGCACG	35	1.2109194E-7	45.0	1
GTATGCG	35	1.2109194E-7	45.0	1
CGTTTAC	20	7.031176E-4	45.0	33
CGTTTTT	790	0.0	39.588608	1
TCACGCC	35	6.244385E-6	38.571426	32
ACCGATC	35	6.244385E-6	38.571426	10
CACCGCT	35	6.244385E-6	38.571426	40
TATACGG	35	6.244385E-6	38.571426	2
ACGGGAT	360	0.0	38.125	5
TCACGAC	30	1.1394133E-4	37.499996	25
GTCCGAA	30	1.1394133E-4	37.499996	9
ACGACAA	30	1.1394133E-4	37.499996	32
TACGGGA	290	0.0	37.24138	4
GCTTGCG	55	2.744855E-9	36.818184	1
TTTACGG	80	0.0	36.5625	2
ACGGGAG	310	0.0	36.29032	5
ATTTACG	25	0.0021064822	36.0	1
TAATACG	25	0.0021064822	36.0	4
CGAACTA	25	0.0021064822	36.0	39
>>END_MODULE