##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	385836
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5681688593081	31.0	31.0	34.0	30.0	34.0
2	31.70028198509211	31.0	31.0	34.0	30.0	34.0
3	31.664738386257373	31.0	31.0	34.0	30.0	34.0
4	35.43904404980354	37.0	35.0	37.0	33.0	37.0
5	31.064752900195938	35.0	35.0	37.0	0.0	37.0
6	33.25595330658622	36.0	35.0	37.0	17.0	37.0
7	29.606138359302918	35.0	32.0	37.0	0.0	37.0
8	32.62629977503395	35.0	33.0	37.0	17.0	37.0
9	36.38986771581708	39.0	35.0	39.0	32.0	39.0
10	36.581944142070725	38.0	35.0	39.0	32.0	39.0
11	36.67300096414021	38.0	35.0	39.0	32.0	39.0
12	36.740480411366484	39.0	35.0	39.0	33.0	39.0
13	36.53003348572969	39.0	35.0	39.0	32.0	39.0
14	37.599959568314	39.0	36.0	41.0	33.0	41.0
15	37.69864139167937	39.0	36.0	41.0	33.0	41.0
16	37.776000684228535	39.0	36.0	41.0	33.0	41.0
17	37.760242693787	39.0	36.0	41.0	33.0	41.0
18	37.69414207072435	39.0	36.0	41.0	33.0	41.0
19	37.63174509377041	39.0	36.0	41.0	33.0	41.0
20	37.56284794575934	39.0	36.0	41.0	32.0	41.0
21	37.46650908676225	39.0	36.0	40.0	32.0	41.0
22	37.432103277039985	39.0	36.0	40.0	32.0	41.0
23	37.31509242268736	39.0	35.0	40.0	32.0	41.0
24	37.21219378181404	39.0	35.0	40.0	32.0	41.0
25	36.95266382608155	39.0	35.0	40.0	31.0	41.0
26	36.56374729159539	39.0	35.0	40.0	31.0	41.0
27	36.49915767320831	38.0	35.0	40.0	30.0	41.0
28	36.23499362423413	38.0	35.0	40.0	30.0	41.0
29	36.195339470655924	38.0	35.0	40.0	30.0	41.0
30	35.97125721809266	38.0	35.0	40.0	29.0	41.0
31	35.742864844130665	38.0	34.0	40.0	28.0	41.0
32	35.686750329155394	38.0	34.0	40.0	28.0	41.0
33	35.50809411252449	38.0	34.0	40.0	27.0	41.0
34	35.512979607916314	38.0	34.0	40.0	27.0	41.0
35	35.49605791061487	38.0	34.0	40.0	27.0	41.0
36	35.38922236390591	38.0	34.0	40.0	27.0	41.0
37	35.30211281477104	38.0	34.0	40.0	26.0	41.0
38	35.176147378679026	38.0	34.0	40.0	25.0	41.0
39	35.12059009527364	38.0	34.0	40.0	25.0	41.0
40	34.97153972153972	38.0	34.0	40.0	25.0	41.0
41	34.848837329849985	38.0	33.0	40.0	25.0	41.0
42	34.80492489036793	38.0	33.0	40.0	25.0	41.0
43	34.56063975367773	38.0	33.0	40.0	24.0	41.0
44	34.33128842306058	37.0	33.0	40.0	23.0	41.0
45	34.24199659959154	37.0	33.0	40.0	23.0	41.0
46	33.96193978788916	37.0	33.0	40.0	22.0	41.0
47	33.799277413201466	37.0	32.0	40.0	21.0	41.0
48	33.68048600959993	37.0	32.0	40.0	21.0	41.0
49	33.431688593081	37.0	32.0	40.0	19.0	41.0
50	33.182963746254885	37.0	31.0	40.0	18.0	41.0
51	30.407535844244705	34.0	26.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	7.0
10	7.0
11	6.0
12	5.0
13	2.0
14	3.0
15	10.0
16	26.0
17	48.0
18	111.0
19	264.0
20	528.0
21	981.0
22	1485.0
23	2221.0
24	3108.0
25	4248.0
26	5481.0
27	6590.0
28	7968.0
29	9639.0
30	11760.0
31	14418.0
32	17970.0
33	23781.0
34	31701.0
35	39036.0
36	42800.0
37	55867.0
38	59440.0
39	46312.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.13622368052748	22.320623269990357	26.53821831037021	11.004934739111954
2	28.678765071170137	25.761981774640002	28.276003276003276	17.283249878186588
3	29.877720067593483	25.897790771208495	28.244901979079195	15.979587182118829
4	26.453726453726457	25.94833037871013	28.746151214505645	18.851791953057777
5	20.30707347163043	39.22806581034429	23.259882437097627	17.204978280927648
6	24.38341678848008	34.752070828020194	26.38167511585233	14.482837267647394
7	69.7731678744337	17.702339854238588	7.816533449444842	4.7079588218828725
8	80.94319866471766	3.258897562695031	9.189914886117418	6.6079888864699
9	75.50954291460621	5.649291408785079	11.172104210078894	7.669061466529821
10	36.77624690282918	33.440114452772676	17.3895126426772	12.394126001720938
11	24.36682943012057	24.196290651986853	31.33922184555096	20.097658072341616
12	23.195606423454525	22.51604308566334	34.07069324790844	20.2176572429737
13	20.099990669610925	26.98244850143584	35.93417928860967	16.983381540343565
14	17.762987383240546	30.745705429249732	31.384577903565248	20.106729283944475
15	14.773116038938824	28.18684622482091	38.9525601550918	18.087477581148466
16	18.149420997522263	28.744336972185074	32.99225577706591	20.11398625322676
17	17.702599031712957	27.526462020132907	29.279797634228018	25.491141313926125
18	18.676069625436714	27.56611617371111	33.31285831285831	20.444955887993864
19	18.858271389916958	29.204376989187114	28.81120476057185	23.126146860324077
20	19.69074943758488	31.039094330233567	30.065364559035444	19.204791673146104
21	19.08168237282161	29.834437429374137	31.960729429083862	19.12315076872039
22	17.831151058999158	26.731305528773884	28.778807576275934	26.658735835951024
23	16.145201588239562	28.892327310048827	30.431841507790875	24.530629593920732
24	19.766429260100146	26.53536735815217	30.906913818306226	22.79128956344146
25	16.505458277610177	30.222685285976425	28.90321276397226	24.36864367244114
26	16.88722669735328	29.069604702516095	30.73300573300573	23.31016286712489
27	20.19718222249868	27.30071843995895	29.703293627344262	22.798805710198113
28	14.930177588405435	26.758519163582456	32.87303413885692	25.438269109155186
29	17.077203786064548	23.889424522335915	32.923832923832926	26.109538767766615
30	19.45437958096186	25.757316580101392	30.25249069552867	24.53581314340808
31	18.40185985755606	26.055889030572576	29.75201899252532	25.790232119346047
32	18.09447537295639	25.61632403404555	29.898454265542874	26.39074632745519
33	17.616292932748628	24.361905058107588	30.325578743300262	27.696223265843518
34	15.438424615639807	22.874485532713383	33.0430545620419	28.644035289604908
35	14.771301796618253	22.904290942265625	33.075451746337826	29.248955514778302
36	18.189075151100468	22.267751065219418	32.858001845343615	26.685171938336495
37	15.649654257249193	24.122943426740896	34.1201961455126	26.10720617049731
38	16.12576327766201	25.379694999948164	31.335075005961084	27.159466716428742
39	17.842295690396956	23.502213375631097	30.929980613524915	27.72551032044703
40	19.03710384723043	22.427404389429707	32.91268829243513	25.62280347090474
41	16.145979120662666	23.57348718108212	30.395556661379448	29.88497703687577
42	18.355207912169938	22.33798816077297	33.05601343576027	26.250790491296822
43	20.28296996651427	22.209695310961134	31.022247794399693	26.4850869281249
44	18.04419494292912	23.89771820151567	29.907266299671363	28.150820555883847
45	18.845053338724224	22.516561440612072	29.553489047159932	29.084896173503765
46	22.03500969323754	22.91750899345836	29.887568811619445	25.159912501684655
47	15.522398117334827	23.252625467815342	34.55172664033424	26.673249774515597
48	17.011631885049606	22.056521423610032	32.33575923449341	28.596087456846952
49	18.615162918960387	19.69074943758488	34.8044246778424	26.88966296561233
50	17.84281404534569	20.494199608123658	33.271649094433904	28.391337252096744
51	16.831503540364302	20.56754683336962	30.34916389346769	32.251785732798396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	298.0
2	445.0
3	787.5
4	1130.0
5	886.5
6	643.0
7	665.0
8	687.0
9	885.0
10	1083.0
11	1291.5
12	1500.0
13	1528.5
14	1557.0
15	1479.5
16	1402.0
17	1337.5
18	1273.0
19	1245.0
20	1217.0
21	1277.5
22	1338.0
23	1654.5
24	1971.0
25	2134.0
26	2876.0
27	3455.0
28	4324.0
29	5193.0
30	5854.5
31	6516.0
32	7378.0
33	8240.0
34	9042.0
35	9844.0
36	11032.5
37	12221.0
38	13751.5
39	15282.0
40	17996.0
41	20710.0
42	24459.5
43	28209.0
44	33379.0
45	38549.0
46	44785.0
47	51021.0
48	51709.0
49	52397.0
50	47960.0
51	43523.0
52	34805.5
53	26088.0
54	20968.0
55	15848.0
56	13091.5
57	10335.0
58	9076.5
59	7818.0
60	6479.5
61	5141.0
62	4483.5
63	3826.0
64	2901.5
65	1977.0
66	1600.0
67	1223.0
68	913.0
69	603.0
70	490.0
71	377.0
72	299.0
73	221.0
74	213.0
75	170.5
76	136.0
77	101.0
78	66.0
79	56.0
80	46.0
81	39.0
82	32.0
83	26.5
84	21.0
85	15.5
86	10.0
87	7.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	385836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.803137005122466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00344888519398	34.811963821503625
2	10.163363639116831	9.310278743906965
3	4.046747432217107	5.560611811889031
4	2.205647647728647	4.041023255757652
5	1.4073615575394787	3.223078711786164
6	0.9559326032375001	2.627082719625036
7	0.7004359753065129	2.245751545819655
8	0.5707790169042393	2.091477560873119
9	0.40511583824158726	1.6700018617732009
>10	3.441890197030243	28.78484317574535
>50	0.06317640476250203	1.9617583420386282
>100	0.031588202381251	2.148038832570677
>500	0.003948525297656375	1.2574579021074674
>1k	5.640750425223392E-4	0.2666317146034326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	1032	0.26747115354710294	TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	916	0.23740656652049058	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	791	0.2050093822245721	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	722	0.1871261364932251	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	672	0.17416726277485772	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	661	0.17131631055681687	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.1518779999792658	No Hit
TCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	519	0.1345131091966535	TruSeq Adapter, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3146414538819602	0.0
2	0.0	0.0	0.0	1.5796867062689848	0.0
3	0.0	0.0	0.0	2.2672845457655586	0.0
4	0.0	0.0	0.0	3.173368996153806	0.0
5	0.0	0.0	0.0	5.844452046983693	0.0
6	0.0	0.0	0.0	7.380337760084595	0.0
7	0.0	0.0	0.0	8.998901087508683	0.0
8	0.0	0.0	0.0	11.767434868700692	0.0
9	0.0	0.0	0.0	12.716542779833919	0.0
10	0.0	0.0	0.0	15.02270394675458	0.0
11	0.0	0.0	0.0	17.881431489026426	0.0
12	0.0	0.0	0.0	20.031567816377944	0.0
13	0.0	0.0	0.0	20.76192993914513	0.0
14	0.0	0.0	0.0	21.00529758757607	0.0
15	0.0	0.0	0.0	21.524170891259498	0.0
16	0.0	0.0	0.0	22.683471734104646	0.0
17	0.0	0.0	0.0	24.190588747550773	0.0
18	0.0	0.0	0.0	25.729325412869716	0.0
19	0.0	0.0	0.0	26.682061808644086	0.0
20	0.0	0.0	0.0	27.454151504784416	0.0
21	0.0	0.0	0.0	28.48360443297152	0.0
22	0.0	0.0	0.0	29.503208617132668	0.0
23	0.0	0.0	0.0	30.577240070910957	0.0
24	0.0	0.0	0.0	31.393130760219368	0.0
25	0.0	0.0	0.0	32.0540333198561	0.0
26	0.0	0.0	0.0	32.67683679076084	0.0
27	0.0	0.0	0.0	33.3201152821406	0.0
28	0.0	0.0	0.0	33.95535927181497	0.0
29	0.0	0.0	0.0	34.59967447309219	0.0
30	0.0	0.0	0.0	35.326926466166974	0.0
31	0.0	0.0	0.0	36.01063664354803	0.0
32	0.0	0.0	0.0	36.59145336360526	0.0
33	0.0	0.0	0.0	37.17123337376502	0.0
34	0.0	0.0	0.0	37.75205009382225	0.0
35	0.0	0.0	0.0	38.40154884458682	0.0
36	0.0	0.0	0.0	38.98210638716968	0.0
37	0.0	0.0	0.0	39.55074176593164	0.0
38	0.0	0.0	0.0	40.12067303206544	0.0
39	0.0	0.0	0.0	40.7655065882914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	35	1.2091186E-7	45.000004	15
GGCGTCA	60	0.0	45.000004	36
ATTACGG	30	2.161738E-6	45.000004	2
AGGCGTC	60	0.0	45.000004	35
ATCCGGG	35	1.2091186E-7	45.000004	3
AACGCAT	35	1.2091186E-7	45.000004	14
TTAACGG	30	2.161738E-6	45.000004	2
ACGTCCG	30	2.161738E-6	45.000004	23
CGTCATA	60	0.0	45.000004	38
AGTACGG	30	2.161738E-6	45.000004	2
TCACGAC	20	7.027454E-4	45.0	25
ACAACGG	20	7.027454E-4	45.0	2
CGCGGGC	25	3.8859722E-5	45.0	4
CGTTGAG	20	7.027454E-4	45.0	2
ATTCGTG	20	7.027454E-4	45.0	1
GTTAGCG	20	7.027454E-4	45.0	1
GTCAGAT	20	7.027454E-4	45.0	10
GGGACGA	45	3.8380676E-10	45.0	7
GCACAAG	20	7.027454E-4	45.0	15
CTACGTA	20	7.027454E-4	45.0	36
>>END_MODULE