FastQCFastQC Report
Sat 14 Jan 2017
SRR2936207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497753
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35580.7148123667762927No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.20753265173690566No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.1474626973619446No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.1024604572950841No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG207.0294284E-445.020
TTCACGG453.8380676E-1045.02
GCACGTA207.0294284E-445.029
ACTACGG207.0294284E-445.02
GCGATCA351.2100645E-745.09
CACGTAT207.0294284E-445.030
TGTGACG302.163013E-644.9999961
GTTACGG302.163013E-644.9999962
CGTTATT4250.044.470591
CGTTTTT17900.041.8575441
CTAACGG556.002665E-1140.9090922
TACGGGT403.4529512E-739.3754
ACGGGCT800.039.3755
TGTAGCG403.4529512E-739.3751
TCAATCT356.2411546E-638.57142628
GCGATCG356.2411546E-638.5714269
ATCGCGG356.2411546E-638.5714262
CGGTTTT4500.038.5000041
TTACGGG1300.038.076923
CGTTTTA2600.038.076921
Produced by FastQC (version 0.11.5)