##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936207.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	497753
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.964367869204203	33.0	31.0	34.0	30.0	34.0
2	32.093136555681234	33.0	31.0	34.0	30.0	34.0
3	31.766412256681527	33.0	31.0	34.0	30.0	34.0
4	35.6375732542044	37.0	35.0	37.0	33.0	37.0
5	31.149306985593256	37.0	35.0	37.0	0.0	37.0
6	33.425833696632665	37.0	35.0	37.0	17.0	37.0
7	29.57160680096353	35.0	32.0	37.0	0.0	37.0
8	32.672237033227326	35.0	35.0	37.0	17.0	37.0
9	36.63556020757283	39.0	35.0	39.0	32.0	39.0
10	36.96551301549162	39.0	37.0	39.0	34.0	39.0
11	37.04644070452614	39.0	37.0	39.0	34.0	39.0
12	37.053297519050616	39.0	37.0	39.0	34.0	39.0
13	36.90144911231072	39.0	37.0	39.0	33.0	39.0
14	38.07850480057378	40.0	37.0	41.0	33.0	41.0
15	38.199390058924806	40.0	37.0	41.0	33.0	41.0
16	38.27077285320229	40.0	37.0	41.0	33.0	41.0
17	38.26609382565248	40.0	37.0	41.0	34.0	41.0
18	38.15652944331827	40.0	37.0	41.0	34.0	41.0
19	38.01984719328663	40.0	37.0	41.0	34.0	41.0
20	37.85245493246651	40.0	36.0	41.0	33.0	41.0
21	37.750478651057854	40.0	36.0	41.0	33.0	41.0
22	37.72107651787131	39.0	35.0	41.0	33.0	41.0
23	37.67608030489018	39.0	35.0	41.0	33.0	41.0
24	37.61422834216971	39.0	35.0	41.0	33.0	41.0
25	37.45949095233981	39.0	35.0	41.0	33.0	41.0
26	37.286774765797496	39.0	35.0	41.0	33.0	41.0
27	37.251150671115994	39.0	35.0	41.0	33.0	41.0
28	37.131157421452	39.0	35.0	41.0	32.0	41.0
29	37.06361990786595	39.0	35.0	41.0	32.0	41.0
30	36.91254497712721	39.0	35.0	41.0	31.0	41.0
31	36.73410908623353	39.0	35.0	41.0	31.0	41.0
32	36.55682035065585	39.0	35.0	41.0	31.0	41.0
33	36.30749588651399	39.0	35.0	41.0	30.0	41.0
34	36.04675411298375	39.0	35.0	40.0	29.0	41.0
35	35.80260088839243	39.0	35.0	40.0	27.0	41.0
36	35.59823647471738	39.0	35.0	40.0	26.0	41.0
37	35.44810176935147	38.0	35.0	40.0	25.0	41.0
38	35.22606192227872	38.0	34.0	40.0	24.0	41.0
39	35.13754412329006	38.0	34.0	40.0	24.0	41.0
40	34.9791221750547	38.0	34.0	40.0	23.0	41.0
41	34.85752572058832	38.0	34.0	40.0	23.0	41.0
42	34.796443215811856	38.0	34.0	40.0	23.0	41.0
43	34.681930596098866	38.0	34.0	40.0	23.0	41.0
44	34.49241089456015	38.0	34.0	40.0	22.0	41.0
45	34.36974563689219	38.0	33.0	40.0	22.0	41.0
46	34.324008092367094	38.0	33.0	40.0	22.0	41.0
47	34.18641374336267	37.0	33.0	40.0	21.0	41.0
48	34.081301368349365	37.0	33.0	40.0	20.0	41.0
49	33.970419063270334	37.0	33.0	40.0	20.0	41.0
50	33.79864712015799	37.0	33.0	40.0	20.0	41.0
51	31.993828264219403	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	20.0
10	27.0
11	17.0
12	11.0
13	14.0
14	18.0
15	35.0
16	55.0
17	160.0
18	295.0
19	496.0
20	853.0
21	1380.0
22	2102.0
23	3249.0
24	4704.0
25	6649.0
26	8662.0
27	8998.0
28	9117.0
29	9361.0
30	10856.0
31	13272.0
32	16845.0
33	24291.0
34	34833.0
35	40560.0
36	47974.0
37	74803.0
38	91762.0
39	86312.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.993719776676386	19.23664950286588	24.193123898801215	14.576506821656524
2	35.46658684126464	22.866361428258593	25.035509580052757	16.631542150424007
3	32.41868959102205	22.791223759575534	29.061602843177237	15.728483806225176
4	27.107219845987867	25.631588358081213	29.41639728941865	17.844794506512265
5	21.189224374338274	39.019955680829646	24.234108081719246	15.55671186311283
6	26.337761901987534	34.138418050719935	26.836603697014382	12.687216350278149
7	70.82368162522376	18.321938792935452	8.178353520722126	2.6760260611186673
8	86.51439569424996	3.048700861672356	8.508637818355691	1.9282656257219946
9	81.40704325237618	5.2365329792085635	10.157648472234222	3.1987752961810374
10	42.51506269173667	28.37752861358947	17.1014539339793	12.00595476069456
11	30.141053896209563	25.502608723603874	27.523088760891447	16.833248619295112
12	27.714549184033043	22.87620566827322	31.302071509363078	18.10717363833066
13	24.876193614101773	22.927435896920763	33.188951146452155	19.00741934252531
14	21.287064065912208	25.137568231632955	33.083678049152894	20.49168965330194
15	20.986111585465082	25.33425212906803	34.62741560573216	19.052220679734727
16	26.25800346758332	23.937575464135826	31.032058068961916	18.77236299931894
17	26.02776879295554	23.59624150934299	30.143464730498863	20.232524967202608
18	25.974529535733588	23.76861616102766	30.83195882295034	19.424895480288416
19	25.31014378617507	25.842536358394625	28.938449391565698	19.90887046386461
20	25.469258849268613	26.745594702593458	29.255675003465576	18.529471444672357
21	26.028773307242748	24.82134713402029	30.68851418273722	18.46136537599974
22	25.003967831434466	22.98549682272131	31.42060419525347	20.589931150590754
23	23.480923269171658	24.36369042476891	31.45536038959082	20.700025916468608
24	23.15325070868483	25.26795418611239	31.673139087057233	19.905656018145546
25	23.7386816352689	25.622346826638914	30.22603580490725	20.412935733184934
26	22.49870919914094	26.5961229766571	29.403539506542405	21.501628317659563
27	22.167018581505285	25.55845971797257	31.181730697755718	21.092791002766432
28	21.22458327724795	26.557147822313475	30.799412560044843	21.41885634039373
29	22.94109729122677	25.258511751812645	30.371690376552223	21.428700580408353
30	23.86464772688462	24.44003351059662	31.04953661755931	20.645782144959448
31	23.944205258431392	25.40878708917877	30.212575313458682	20.434432338931156
32	24.96860892852479	25.013209362876772	29.27737251206924	20.740809196529202
33	24.750026619628613	25.19562915743351	29.309316066402413	20.74502815653547
34	22.80287612530713	25.855795946985754	30.48640590815123	20.854922019555886
35	23.53054627495967	26.357852187731666	29.40173138082543	20.709870156483234
36	24.591916070822275	27.300287491989	27.636197069630917	20.471599367557804
37	23.676602652319524	27.254280737634932	28.21921716192569	20.849899448119853
38	24.22245571598765	26.634495422428394	28.767882865598	20.37516599598596
39	24.019744732829334	26.67347057677201	27.480296452256443	21.82648823814221
40	25.16308289452801	25.490755455014835	28.71424180266116	20.631919847795995
41	22.465962033378002	25.914459581358624	28.49425317376289	23.125325211500485
42	22.514379622021362	26.24293575327522	28.980237185913495	22.262447438789923
43	23.022663851347957	25.72199464392982	29.678173712664712	21.577167792057505
44	23.29689625175539	26.03198775296181	28.83096636283458	21.84014963244822
45	22.427589587606704	25.447561340664947	28.55693486528459	23.567914206443756
46	23.196043017319834	25.823048781222813	28.651961916854347	22.328946284603006
47	21.8220683752785	26.708025868251923	29.745677072765	21.72422868370457
48	22.681731702270003	26.190701010340472	29.583950272524728	21.5436170148648
49	22.635724947915932	26.46332618788837	28.882598397197	22.018350466998694
50	22.413928193300695	26.124001261669942	29.56908346107407	21.892987083955294
51	22.47540446767774	25.832692118380002	28.63850142540577	23.053401988536482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	407.5
2	662.0
3	4306.0
4	7950.0
5	6096.5
6	4243.0
7	3399.5
8	2556.0
9	2424.5
10	2293.0
11	2292.5
12	2292.0
13	2238.5
14	2185.0
15	2080.5
16	1976.0
17	1921.5
18	1867.0
19	1882.0
20	1897.0
21	2087.0
22	2277.0
23	2502.5
24	2728.0
25	3066.0
26	4133.5
27	4863.0
28	5885.5
29	6908.0
30	7957.5
31	9007.0
32	10464.5
33	11922.0
34	13838.0
35	15754.0
36	17113.0
37	18472.0
38	19137.5
39	19803.0
40	21489.5
41	23176.0
42	24800.0
43	26424.0
44	27628.5
45	28833.0
46	30714.0
47	32595.0
48	34112.0
49	35629.0
50	35335.5
51	35042.0
52	33484.0
53	31926.0
54	29987.5
55	28049.0
56	27258.5
57	26468.0
58	25590.5
59	24713.0
60	22883.0
61	21053.0
62	18829.5
63	16606.0
64	14845.0
65	13084.0
66	11504.0
67	9924.0
68	8539.0
69	7154.0
70	6063.0
71	4972.0
72	4221.0
73	3470.0
74	2881.5
75	1839.5
76	1386.0
77	1097.0
78	808.0
79	613.0
80	418.0
81	334.5
82	251.0
83	185.5
84	120.0
85	96.5
86	73.0
87	61.5
88	50.0
89	32.0
90	14.0
91	10.5
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	497753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.22544621643863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.95832171884275	28.840630294124626
2	9.957191454090886	8.209793215148123
3	5.117500736464263	6.329137541210776
4	3.1754077441836004	5.2363040469241495
5	2.1777494524144534	4.488934646169536
6	1.5749943964938218	3.8957908070308966
7	1.2444768052299302	3.59128781211283
8	1.0541201486619087	3.476525879546065
9	0.8507841635373223	3.156656109813505
>10	4.856868241787547	30.516730226490434
>50	0.026262648773974093	0.7107655508342118
>100	0.0043771081289167	0.37753780880153004
>500	9.726906953148223E-4	0.2494195614174739
>1k	9.726906953148223E-4	0.9204865003759025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3558	0.7148123667762927	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1033	0.20753265173690566	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	734	0.1474626973619446	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.1024604572950841	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0090285744134138E-4	0.0	0.0	0.02611737146737438	0.0
2	2.0090285744134138E-4	0.0	0.0	0.07332954296608961	0.0
3	2.0090285744134138E-4	0.0	0.0	0.12636789733060375	0.0
4	2.0090285744134138E-4	0.0	0.0	0.18382611455882736	0.0
5	2.0090285744134138E-4	0.0	0.0	0.3835235548555207	0.0
6	2.0090285744134138E-4	0.0	0.0	0.6264151095021024	0.0
7	2.0090285744134138E-4	0.0	0.0	0.8269161612285612	0.0
8	2.0090285744134138E-4	0.0	0.0	1.1648347674448973	0.0
9	2.0090285744134138E-4	0.0	0.0	1.3818098534815462	0.0
10	2.0090285744134138E-4	0.0	0.0	1.7267600597083292	0.0
11	2.0090285744134138E-4	0.0	0.0	2.078942768803001	0.0
12	2.0090285744134138E-4	0.0	0.0	2.3549832949274037	0.0
13	2.0090285744134138E-4	0.0	0.0	2.5008387694298175	0.0
14	2.0090285744134138E-4	0.0	0.0	2.613947078169293	0.0
15	2.0090285744134138E-4	0.0	0.0	2.717612952609025	0.0
16	2.0090285744134138E-4	0.0	0.0	2.8919966328681093	0.0
17	2.0090285744134138E-4	0.0	0.0	3.088479627445741	0.0
18	2.0090285744134138E-4	0.0	0.0	3.297217696327295	0.0
19	2.0090285744134138E-4	0.0	0.0	3.435037056532055	0.0
20	2.0090285744134138E-4	0.0	0.0	3.5829015596088825	0.0
21	2.0090285744134138E-4	0.0	0.0	3.7655222570230618	0.0
22	2.0090285744134138E-4	0.0	0.0	3.964014280175107	0.0
23	2.0090285744134138E-4	0.0	0.0	4.169939709052482	0.0
24	2.0090285744134138E-4	0.0	0.0	4.3399035264478565	0.0
25	2.0090285744134138E-4	0.0	0.0	4.505648383836963	0.0
26	2.0090285744134138E-4	0.0	0.0	4.657731846920059	0.0
27	2.0090285744134138E-4	0.0	0.0	4.833320944323791	0.0
28	2.0090285744134138E-4	0.0	0.0	5.015539836023088	0.0
29	2.0090285744134138E-4	0.0	0.0	5.206196647734921	0.0
30	2.0090285744134138E-4	0.0	0.0	5.525431288209212	0.0
31	2.0090285744134138E-4	0.0	0.0	5.758076797126286	0.0
32	2.0090285744134138E-4	0.0	0.0	5.995945780336834	0.0
33	2.0090285744134138E-4	0.0	0.0	6.216537117807427	0.0
34	2.0090285744134138E-4	0.0	0.0	6.436324843848254	0.0
35	2.0090285744134138E-4	0.0	0.0	6.695288627090143	0.0
36	2.0090285744134138E-4	0.0	0.0	6.9194962159946805	0.0
37	2.0090285744134138E-4	0.0	0.0	7.162387770641262	0.0
38	2.0090285744134138E-4	0.0	0.0	7.466554696807453	0.0
39	2.0090285744134138E-4	0.0	0.0	7.949726068953879	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	20	7.0294284E-4	45.0	20
TTCACGG	45	3.8380676E-10	45.0	2
GCACGTA	20	7.0294284E-4	45.0	29
ACTACGG	20	7.0294284E-4	45.0	2
GCGATCA	35	1.2100645E-7	45.0	9
CACGTAT	20	7.0294284E-4	45.0	30
TGTGACG	30	2.163013E-6	44.999996	1
GTTACGG	30	2.163013E-6	44.999996	2
CGTTATT	425	0.0	44.47059	1
CGTTTTT	1790	0.0	41.857544	1
CTAACGG	55	6.002665E-11	40.909092	2
TACGGGT	40	3.4529512E-7	39.375	4
ACGGGCT	80	0.0	39.375	5
TGTAGCG	40	3.4529512E-7	39.375	1
TCAATCT	35	6.2411546E-6	38.571426	28
GCGATCG	35	6.2411546E-6	38.571426	9
ATCGCGG	35	6.2411546E-6	38.571426	2
CGGTTTT	450	0.0	38.500004	1
TTACGGG	130	0.0	38.07692	3
CGTTTTA	260	0.0	38.07692	1
>>END_MODULE