FastQCFastQC Report
Sat 14 Jan 2017
SRR2936206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848786
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92921.094740016918281No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.3283513158793854No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.223966936306678No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.1544558934760941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT8150.044.1717761
CGTTTTT36350.042.647871
CGGTTTT10200.042.1323551
TACGAAT501.0804797E-940.50000412
CGTTTTA5550.040.1351361
GTACGAG451.9272193E-840.01
CGATAGA403.4567893E-739.37510
TTACGGG2750.039.2727243
TTTGACG659.094947E-1238.076921
ACGACCA1850.037.70270528
TCGCATA301.1396703E-437.5000041
GCGACGA301.1396703E-437.5000041
AATTGCG601.546141E-1037.5000041
TATAGCG301.1396703E-437.5000041
ACGGGAT3400.037.0588235
CGAATAT552.746674E-936.8181814
TACGGGA3800.036.118424
TAATACG250.002106798336.0000041
TTATACG250.002106798336.0000041
CACGACC1950.035.7692327
Produced by FastQC (version 0.11.5)