##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936205.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772632
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91377266279419	33.0	31.0	34.0	30.0	34.0
2	32.03598737820851	33.0	31.0	34.0	30.0	34.0
3	31.706127884943932	33.0	31.0	34.0	30.0	34.0
4	35.580817258410214	37.0	35.0	37.0	33.0	37.0
5	31.129674929332463	37.0	35.0	37.0	0.0	37.0
6	33.40294085670798	37.0	35.0	37.0	17.0	37.0
7	29.560679599084686	35.0	32.0	37.0	0.0	37.0
8	32.6425775789768	35.0	35.0	37.0	17.0	37.0
9	36.56958163783017	39.0	35.0	39.0	32.0	39.0
10	36.92183212706696	38.0	37.0	39.0	34.0	39.0
11	37.02043146025534	39.0	37.0	39.0	34.0	39.0
12	37.04673764482962	39.0	37.0	39.0	33.0	39.0
13	36.94132005922613	39.0	37.0	39.0	33.0	39.0
14	38.23594544362646	40.0	38.0	41.0	33.0	41.0
15	38.30645248967167	40.0	38.0	41.0	34.0	41.0
16	38.33325179386823	40.0	38.0	41.0	34.0	41.0
17	38.29013424243365	40.0	37.0	41.0	34.0	41.0
18	38.181690636680855	40.0	37.0	41.0	34.0	41.0
19	38.03082580063989	40.0	37.0	41.0	34.0	41.0
20	37.88279543171911	40.0	36.0	41.0	33.0	41.0
21	37.759792501475474	40.0	36.0	41.0	33.0	41.0
22	37.71329300365504	39.0	36.0	41.0	33.0	41.0
23	37.63315006367844	39.0	35.0	41.0	33.0	41.0
24	37.56460902473623	39.0	35.0	41.0	33.0	41.0
25	37.416832334151316	39.0	35.0	41.0	33.0	41.0
26	37.24719271270152	39.0	35.0	41.0	32.0	41.0
27	37.19334819163586	39.0	35.0	41.0	32.0	41.0
28	37.0383222543203	39.0	35.0	41.0	32.0	41.0
29	37.0011842636598	39.0	35.0	41.0	32.0	41.0
30	36.84537528862382	39.0	35.0	41.0	31.0	41.0
31	36.69662400728937	39.0	35.0	41.0	31.0	41.0
32	36.51295571501051	39.0	35.0	41.0	30.0	41.0
33	36.26592349268474	39.0	35.0	40.0	30.0	41.0
34	36.007924859441495	39.0	35.0	40.0	29.0	41.0
35	35.77173220886528	39.0	35.0	40.0	27.0	41.0
36	35.56379880719411	38.0	35.0	40.0	26.0	41.0
37	35.426892751012126	38.0	35.0	40.0	25.0	41.0
38	35.265434255894135	38.0	34.0	40.0	25.0	41.0
39	35.237454570869446	38.0	34.0	40.0	24.0	41.0
40	35.07287686764204	38.0	34.0	40.0	24.0	41.0
41	34.95475853964112	38.0	34.0	40.0	24.0	41.0
42	34.840669296637984	38.0	34.0	40.0	23.0	41.0
43	34.6786568508682	38.0	34.0	40.0	23.0	41.0
44	34.51648909183156	38.0	34.0	40.0	22.0	41.0
45	34.37258617297756	38.0	33.0	40.0	22.0	41.0
46	34.25083610308659	37.0	33.0	40.0	22.0	41.0
47	34.09374579359902	37.0	33.0	40.0	21.0	41.0
48	33.98258032284451	37.0	33.0	40.0	20.0	41.0
49	33.82752979426169	37.0	33.0	40.0	20.0	41.0
50	33.66940535727228	37.0	33.0	40.0	20.0	41.0
51	31.784519667836694	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	32.0
10	43.0
11	31.0
12	18.0
13	19.0
14	37.0
15	60.0
16	116.0
17	215.0
18	408.0
19	737.0
20	1196.0
21	2143.0
22	3242.0
23	4802.0
24	7034.0
25	10017.0
26	12564.0
27	13588.0
28	14197.0
29	15095.0
30	17527.0
31	21237.0
32	27136.0
33	38135.0
34	55004.0
35	66089.0
36	76466.0
37	116504.0
38	139916.0
39	128992.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.337790824092195	19.10171983557502	24.110960974953148	15.449528365379637
2	35.65280754615392	23.32093415752907	24.993010902991333	16.033247393325674
3	31.893191066380886	23.323134428809574	28.5203046210874	16.263369883722135
4	27.123650068855547	25.803875583719027	29.060018223423313	18.012456124002114
5	21.28697750028474	39.15965168411352	23.613440810114	15.939930005487735
6	26.39354828689467	35.023012249039645	25.786661696642128	12.796777767423558
7	69.97833379927313	18.625296389484255	8.604613839447499	2.791755971795111
8	84.75017861025688	3.7009857215336663	9.25304672858489	2.295788939624556
9	78.98792698205614	6.214472090205946	11.022194265834187	3.7754066619037263
10	41.616060427215025	27.93192619513559	17.486332432516384	12.965680945133002
11	29.80966359146398	25.457526998622885	27.278186769380508	17.454622640532623
12	27.682518974104102	22.79325733337475	30.74426117478955	18.7799625177316
13	23.65058656643784	23.789850795721637	32.82740036653931	19.732162271301217
14	21.102542995889376	25.338712349475557	32.14117458246617	21.4175700721689
15	21.06966835440417	25.503991550958283	33.53977055053376	19.88656954410379
16	24.279864152662586	23.90879487259135	32.361331138239166	19.4500098365069
17	24.21139688752213	24.276499031880636	30.056093974880664	21.456010105716565
18	24.834203087627746	24.163896913407676	30.64304869588627	20.35885130307831
19	24.9543120139989	26.074250095776513	28.67665330972572	20.294784580498867
20	26.402090516571924	25.11596723925491	29.168996365669553	19.31294587850361
21	26.039304610733183	25.761164435332734	29.137416001408173	19.06211495252591
22	25.56106917652906	23.147371581813854	29.531523416063532	21.760035825593555
23	24.40411476614999	24.97346731691154	29.962776587042733	20.659641329895734
24	23.241983246875613	24.608092856625145	31.253688690087905	20.896235206411333
25	23.485825075844645	25.203460379585625	29.534370825955953	21.776343718613777
26	22.95633626357697	26.24237670715166	28.943921556446018	21.857365472825354
27	22.777596578966442	26.028820965220184	29.75620994211992	21.437372513693454
28	21.66102361797078	26.448166785740174	30.671522794810468	21.21928680147858
29	22.880103335093548	24.892833845867113	30.19043995071392	22.036622868325413
30	23.34203087627745	25.260408577434017	30.064118493668396	21.333442052620136
31	24.412786423549633	25.53168908354818	28.65840400086975	21.39712049203243
32	24.33059981983661	25.957765145632074	28.85202787355429	20.859607160977024
33	24.150565858002256	25.731266631462326	28.03896292154609	22.079204588989324
34	23.000212261464707	26.13935224013502	29.376598432371427	21.483837066028848
35	23.776519740316218	25.778119466964867	28.58074736744013	21.864613425278787
36	23.8836858944491	28.058247652181116	27.391953737354914	20.66611271601487
37	23.937139543793165	27.38988289379679	27.67216475631348	21.000812806096565
38	23.427323745327662	27.417968709553836	27.786449435177417	21.368258109941085
39	23.46278694126052	27.156912993507902	27.51141552511416	21.868884540117417
40	24.18072251731743	25.94042183083279	28.99413951273051	20.88471613911927
41	22.030669193095807	26.36753331469574	28.777477505461853	22.824319986746602
42	22.25690885182079	26.745073980886115	28.222491431884777	22.77552573540832
43	22.47253543731039	26.11126642437797	28.94819267128465	22.468005467026995
44	22.780573416581245	26.125374046117688	28.34518891270359	22.74886362459748
45	23.035804885119955	25.938350987274667	28.26546143571584	22.760382691889543
46	22.972903012041957	26.94167469118545	27.531865105250624	22.553557191521968
47	22.849817248055995	26.032703796891667	29.320038517690183	21.79744043736216
48	22.456745255179698	25.475646879756468	29.35537228590066	22.712235579163174
49	22.155955228362274	26.6089160169395	28.813070129117097	22.42205862558113
50	21.527971919361352	26.190735045920952	29.702756292775863	22.57853674194183
51	21.915996231064724	25.807240704500977	28.68247755723294	23.59428550720136
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	556.0
1	974.5
2	1393.0
3	7859.5
4	14326.0
5	10681.5
6	7037.0
7	5670.5
8	4304.0
9	4078.0
10	3852.0
11	3833.5
12	3815.0
13	3712.0
14	3609.0
15	3560.5
16	3512.0
17	3419.0
18	3326.0
19	3380.5
20	3435.0
21	3447.0
22	3459.0
23	3725.0
24	3991.0
25	4739.0
26	6311.5
27	7136.0
28	8921.5
29	10707.0
30	12062.5
31	13418.0
32	15415.5
33	17413.0
34	19821.5
35	22230.0
36	23650.0
37	25070.0
38	26700.5
39	28331.0
40	31394.0
41	34457.0
42	36463.0
43	38469.0
44	41650.0
45	44831.0
46	47721.5
47	50612.0
48	51915.5
49	53219.0
50	51971.5
51	50724.0
52	49030.5
53	47337.0
54	45400.0
55	43463.0
56	42841.0
57	42219.0
58	41219.5
59	40220.0
60	38582.5
61	36945.0
62	33891.0
63	30837.0
64	26447.0
65	22057.0
66	18812.0
67	15567.0
68	13300.5
69	11034.0
70	9642.0
71	8250.0
72	6976.5
73	5703.0
74	4901.0
75	3374.0
76	2649.0
77	2056.0
78	1463.0
79	1164.5
80	866.0
81	718.0
82	570.0
83	449.5
84	329.0
85	231.5
86	134.0
87	111.5
88	89.0
89	64.0
90	39.0
91	31.5
92	24.0
93	16.5
94	9.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	772632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.3294531290224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.10920201035954	26.196878746065643
2	8.414529644758009	6.113905206640112
3	4.121826147188646	4.492310694608067
4	2.7123684278536158	3.9415544667339244
5	1.9440862279013673	3.531379474766034
6	1.4417632520205625	3.142708229245676
7	1.1320249579498765	2.878809335550651
8	0.92846738541611	2.6984569888240433
9	0.7684835103662001	2.5126727103247917
>10	6.369266474821958	40.96461552331767
>50	0.03926626674134866	0.931238869838277
>100	0.016880822600980786	1.0160301518328998
>500	7.339488087382637E-4	0.22837680751734823
>1k	7.339488087382637E-4	0.42942305721738394
>5k	3.669744043691319E-4	0.9216397375174712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6913	0.8947338448316923	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1897	0.24552438936000578	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1324	0.17136230443471148	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.12049720953830542	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	782	0.10121247890328125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.588554447654252E-4	0.0	0.0	0.03468662959856698	0.0
2	2.588554447654252E-4	0.0	0.0	0.11764979964588575	0.0
3	2.588554447654252E-4	0.0	0.0	0.19685956574410587	0.0
4	2.588554447654252E-4	0.0	0.0	0.2838349951852887	0.0
5	2.588554447654252E-4	0.0	0.0	0.5395841746135288	0.0
6	2.588554447654252E-4	0.0	0.0	0.8667774567970262	0.0
7	2.588554447654252E-4	0.0	0.0	1.103371333312625	0.0
8	2.588554447654252E-4	0.0	0.0	1.5250468528355026	0.0
9	2.588554447654252E-4	0.0	0.0	1.734202052205966	0.0
10	2.588554447654252E-4	0.0	0.0	2.105789043166734	0.0
11	2.588554447654252E-4	0.0	0.0	2.514133507284192	0.0
12	2.588554447654252E-4	0.0	0.0	2.845209621139171	0.0
13	2.588554447654252E-4	0.0	0.0	3.0179956305200926	0.0
14	2.588554447654252E-4	0.0	0.0	3.099793951065967	0.0
15	2.588554447654252E-4	0.0	0.0	3.2134314913179884	0.0
16	2.588554447654252E-4	0.0	0.0	3.4268578055270815	0.0
17	2.588554447654252E-4	0.0	0.0	3.667075658269396	0.0
18	2.588554447654252E-4	0.0	0.0	3.9286490852048583	0.0
19	2.588554447654252E-4	0.0	0.0	4.106353348036323	0.0
20	2.588554447654252E-4	0.0	0.0	4.291564418765985	0.0
21	2.588554447654252E-4	0.0	0.0	4.523628324998188	0.0
22	2.588554447654252E-4	0.0	0.0	4.7576336470661325	0.0
23	2.588554447654252E-4	0.0	0.0	5.015971380942027	0.0
24	2.588554447654252E-4	0.0	0.0	5.224091158533429	0.0
25	2.588554447654252E-4	0.0	0.0	5.414220482713634	0.0
26	2.588554447654252E-4	0.0	0.0	5.595548721771814	0.0
27	2.588554447654252E-4	0.0	0.0	5.791890576626389	0.0
28	2.588554447654252E-4	0.0	0.0	5.986420443367606	0.0
29	2.588554447654252E-4	0.0	0.0	6.1937636546247115	0.0
30	2.588554447654252E-4	0.0	0.0	6.433981507367026	0.0
31	2.588554447654252E-4	0.0	0.0	6.66035059381439	0.0
32	2.588554447654252E-4	0.0	0.0	6.882319137700742	0.0
33	2.588554447654252E-4	0.0	0.0	7.099240000414169	0.0
34	2.588554447654252E-4	0.0	0.0	7.319914267076694	0.0
35	2.588554447654252E-4	0.0	0.0	7.563238385156193	0.0
36	2.588554447654252E-4	0.0	0.0	7.799832261671792	0.0
37	2.588554447654252E-4	0.0	0.0	8.048204060924217	0.0
38	2.588554447654252E-4	0.0	0.0	8.307447788856791	0.0
39	2.588554447654252E-4	0.0	0.0	8.58274055436482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	455	0.0	45.000004	1
TCTACGG	35	1.2112287E-7	45.0	2
GCTACGA	155	0.0	42.09677	10
CGTTTTT	2835	0.0	41.825394	1
TACGAAT	160	0.0	40.781254	12
CGAATAT	160	0.0	40.781254	14
ACGGGCG	145	0.0	40.34483	5
CGTTTTA	385	0.0	40.324677	1
CGGTTTT	755	0.0	39.33775	1
GCGAATG	75	0.0	39.0	1
TACGGGA	300	0.0	39.0	4
AACCGAT	30	1.1395753E-4	37.500004	33
TTTACGG	60	1.546141E-10	37.500004	2
TTAAGCG	30	1.1395753E-4	37.500004	1
GCGTACG	30	1.1395753E-4	37.500004	1
TGCGTAG	30	1.1395753E-4	37.500004	1
AGGCGAT	165	0.0	36.81818	7
GACCTAT	185	0.0	36.48649	33
GGCGATA	235	0.0	36.38298	8
CTACGAA	180	0.0	36.25	11
>>END_MODULE