##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936204.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593835
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.787057010785823	31.0	31.0	34.0	30.0	34.0
2	31.901634292353936	33.0	31.0	34.0	30.0	34.0
3	31.782616383338805	33.0	31.0	34.0	30.0	34.0
4	35.56381991630672	37.0	35.0	37.0	33.0	37.0
5	31.05465154462098	37.0	35.0	37.0	0.0	37.0
6	33.31001372435104	37.0	35.0	37.0	17.0	37.0
7	29.702999991580153	35.0	32.0	37.0	0.0	37.0
8	32.722045686091256	35.0	35.0	37.0	17.0	37.0
9	36.56972728114712	39.0	35.0	39.0	32.0	39.0
10	36.73336027684458	38.0	35.0	39.0	33.0	39.0
11	36.796839189337106	39.0	37.0	39.0	33.0	39.0
12	36.89648471376729	39.0	35.0	39.0	33.0	39.0
13	36.747556139331635	39.0	35.0	39.0	33.0	39.0
14	38.0236260914227	40.0	37.0	41.0	33.0	41.0
15	38.07843424520279	40.0	37.0	41.0	33.0	41.0
16	38.12757584177423	40.0	37.0	41.0	33.0	41.0
17	38.07610194751067	40.0	37.0	41.0	33.0	41.0
18	37.97331918798993	40.0	37.0	41.0	33.0	41.0
19	37.86105904838886	40.0	37.0	41.0	33.0	41.0
20	37.832855927993464	40.0	36.0	41.0	33.0	41.0
21	37.76605454377058	39.0	36.0	41.0	33.0	41.0
22	37.76972559717767	39.0	36.0	41.0	33.0	41.0
23	37.706069867892595	39.0	36.0	41.0	33.0	41.0
24	37.671981274259686	39.0	36.0	41.0	33.0	41.0
25	37.57716369025066	39.0	36.0	41.0	33.0	41.0
26	37.398937415275284	39.0	36.0	41.0	32.0	41.0
27	37.393974757297904	39.0	36.0	41.0	32.0	41.0
28	37.28547660545438	39.0	36.0	41.0	32.0	41.0
29	37.298187206884066	39.0	36.0	41.0	32.0	41.0
30	37.13762577146851	39.0	36.0	41.0	32.0	41.0
31	37.0631623262354	39.0	35.0	41.0	31.0	41.0
32	36.96207027204527	39.0	35.0	41.0	31.0	41.0
33	36.80130002441756	39.0	35.0	41.0	31.0	41.0
34	36.66685695521483	39.0	35.0	41.0	30.0	41.0
35	36.515466417439185	39.0	35.0	40.0	30.0	41.0
36	36.41242432662271	39.0	35.0	40.0	30.0	41.0
37	36.25352833699597	39.0	35.0	40.0	30.0	41.0
38	36.187925939023465	39.0	35.0	40.0	30.0	41.0
39	36.17136241548578	39.0	35.0	40.0	30.0	41.0
40	36.03579445468859	39.0	35.0	40.0	29.0	41.0
41	35.98720856803658	39.0	35.0	40.0	28.0	41.0
42	35.93128057457038	39.0	35.0	40.0	28.0	41.0
43	35.7509392339623	38.0	35.0	40.0	27.0	41.0
44	35.609965731221635	38.0	35.0	40.0	27.0	41.0
45	35.50002273358761	38.0	34.0	40.0	26.0	41.0
46	35.39079373900157	38.0	34.0	40.0	26.0	41.0
47	35.17891333451211	38.0	34.0	40.0	26.0	41.0
48	35.088583529094784	38.0	34.0	40.0	25.0	41.0
49	34.92921939596016	38.0	34.0	40.0	24.0	41.0
50	34.74646997903458	38.0	34.0	40.0	24.0	41.0
51	32.67613562689973	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	10.0
11	12.0
12	4.0
13	5.0
14	3.0
15	24.0
16	46.0
17	110.0
18	252.0
19	418.0
20	716.0
21	1201.0
22	1867.0
23	2683.0
24	3887.0
25	5281.0
26	6502.0
27	7519.0
28	8807.0
29	10367.0
30	13018.0
31	16211.0
32	20992.0
33	29184.0
34	41328.0
35	50130.0
36	59276.0
37	91742.0
38	114480.0
39	107731.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58870730084956	20.3386462569569	25.40992026404641	10.662726178147128
2	31.618210445662516	26.704219185463973	26.007561022843046	15.670009346030463
3	30.659189842296257	26.489681477178006	27.423442538752347	15.427686141773389
4	26.311180715181827	26.45347613394293	29.023213518906765	18.212129631968477
5	21.79191189471823	39.80853267321731	22.170636624651628	16.228918807412835
6	25.257015837732705	37.761162612510205	24.308772638864333	12.673048910892756
7	72.7282831089444	17.702392078607694	6.8434834592100495	2.7258413532378523
8	87.2136199449342	4.021319053272373	6.269249875807254	2.4958111259861746
9	81.62755647612552	6.041408808844208	7.3114585701415375	5.019576144888731
10	37.061978495710086	36.27606995209106	14.944218511876194	11.71773304032265
11	25.6594845369505	26.196839189337105	30.579369690233822	17.564306583478576
12	25.21457980752229	24.54419156836495	31.365446630798115	18.875781993314643
13	21.89732838246314	26.81435078767671	34.41174737090269	16.876573458957456
14	20.46982747732956	29.771737940673752	30.147936716427964	19.610497865568718
15	18.849512069851052	28.094672762636087	35.700657590071316	17.355157577441545
16	22.364629905613512	27.521786354795523	31.4735574696675	18.640026269923464
17	22.487222881776926	26.169558884201837	30.421076561671175	20.922141672350065
18	22.712706391506057	27.134810174543432	31.20075441831485	18.951729015635657
19	21.94060639739995	29.63634679666911	29.031633366170738	19.391413439760203
20	23.467966691084225	29.029780999772665	29.830171680685712	17.6720806284574
21	22.380964409305616	28.76977611626125	30.846615642392244	18.00264383204089
22	21.84849326833211	25.653759040810996	29.874460077294195	22.623287613562688
23	20.030143053205016	28.525768942551384	30.85402510798454	20.590062896259063
24	21.159244571303475	26.642080712655876	31.72598449064134	20.47269022539931
25	19.934998779122147	29.159951838473653	30.08899778558017	20.816051596824035
26	19.989727786337955	30.190204349693094	29.436122828731886	20.383945035237062
27	20.793318009211312	28.92436451202775	29.799018245809023	20.483299232951914
28	18.553470240049847	28.112354441890425	32.35461028736939	20.979565030690342
29	21.546725942391404	26.052017816396813	31.535022354694487	20.8662338865173
30	21.174568693323902	27.451733225559288	31.84672510040668	19.52697298071013
31	22.111024106022718	27.693888032871083	28.46043092778297	21.73465693332323
32	22.813407764783147	28.912913519748752	28.108481312148996	20.165197403319105
33	21.650458460683524	27.210925593809726	29.26856786817887	21.870048077327876
34	21.231150066937783	28.57847718642384	29.131492754721428	21.058879991916946
35	20.75071358205562	28.213055815167515	30.00833564879133	21.027894953985534
36	22.326403799035084	27.35642055453114	29.445216263776974	20.871959382656797
37	21.389948386336272	28.143844670657675	30.48439381309623	19.981813129909824
38	20.701373277088752	28.176008487206044	29.268399471233593	21.85421876447161
39	22.5520557057095	28.24926115840259	28.947098099640474	20.251585036247445
40	21.506310675524347	28.159000395732818	30.21243274646998	20.122256182272853
41	20.59107327793074	28.868119932304424	28.722119780747178	21.81868700901766
42	21.88907693214445	27.66458696439247	29.281534432965383	21.164801670497695
43	22.957555549942324	26.193302853486237	28.794530467217328	22.05461112935411
44	21.246979379794052	27.024678572330696	29.37718389788409	22.35115814999116
45	21.440972660755932	27.253866814855975	28.273678715468105	23.031481808919988
46	22.215261815150672	29.099665732063617	27.73228253639479	20.952789916390916
47	20.044625190499044	27.7747185666052	31.01501258767166	21.165643655224095
48	22.3737233406586	26.481261629914034	29.23219412799852	21.912820901428848
49	21.830643192132495	25.305177364082613	30.834154268441573	22.03002517534332
50	20.912879840359697	26.134363922638443	30.051950457618698	22.900805779383163
51	20.192982899290207	26.170232471982956	29.20642939537077	24.430355233356067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	432.0
2	712.0
3	2106.0
4	3500.0
5	2801.5
6	2103.0
7	1753.0
8	1403.0
9	1471.5
10	1540.0
11	1498.0
12	1456.0
13	1513.5
14	1571.0
15	1541.0
16	1511.0
17	1698.0
18	1885.0
19	1908.0
20	1931.0
21	2245.5
22	2560.0
23	3020.0
24	3480.0
25	4229.5
26	6317.0
27	7655.0
28	8833.5
29	10012.0
30	11928.5
31	13845.0
32	15875.5
33	17906.0
34	19869.5
35	21833.0
36	24267.0
37	26701.0
38	29018.0
39	31335.0
40	33655.5
41	35976.0
42	39044.0
43	42112.0
44	46285.0
45	50458.0
46	56248.0
47	62038.0
48	58800.5
49	55563.0
50	52849.0
51	50135.0
52	43991.5
53	37848.0
54	33583.5
55	29319.0
56	25542.0
57	21765.0
58	19205.0
59	16645.0
60	14488.0
61	12331.0
62	10578.0
63	8825.0
64	7023.5
65	5222.0
66	4107.5
67	2993.0
68	2397.0
69	1801.0
70	1420.5
71	1040.0
72	848.0
73	656.0
74	572.0
75	374.5
76	261.0
77	191.5
78	122.0
79	89.5
80	57.0
81	54.0
82	51.0
83	42.0
84	33.0
85	22.5
86	12.0
87	7.5
88	3.0
89	6.0
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	593835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.441774544799465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72813659367627	29.474103156807573
2	9.191451914556511	7.250543483066066
3	3.7866441862601214	4.480558988275419
4	2.3221154137437487	3.663534104635386
5	1.6054992447073115	3.1661869620795797
6	1.1548269915195328	2.7329055502657473
7	0.8850465671650444	2.4435465014640676
8	0.706778194802155	2.230126897005356
9	0.5397802580266514	1.9160902116738783
>10	4.982287225195916	37.582005132984015
>50	0.07417228085467621	1.8570779805747666
>100	0.020627794077896715	1.6535056735865195
>500	8.777784713998602E-4	0.18124167087992946
>1k	0.0017555569427997204	1.3685736867017404
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCG	2373	0.3996059511480462	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGC	2166	0.36474778347520775	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTC	1804	0.30378808928406037	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1563	0.26320442547172196	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08992396877920635	0.0
2	0.0	0.0	0.0	0.38461862301817845	0.0
3	0.0	0.0	0.0	0.5344919043168557	0.0
4	0.0	0.0	0.0	0.8295233524463866	0.0
5	0.0	0.0	0.0	1.827948841008024	0.0
6	0.0	0.0	0.0	2.2329434944050113	0.0
7	0.0	0.0	0.0	2.700413414500661	0.0
8	0.0	0.0	0.0	3.468471881920062	0.0
9	0.0	0.0	0.0	3.7045643992017987	0.0
10	0.0	0.0	0.0	4.989938282519555	0.0
11	0.0	0.0	0.0	5.946769725597178	0.0
12	0.0	0.0	0.0	7.166132006365404	0.0
13	0.0	0.0	0.0	7.482381469600141	0.0
14	0.0	0.0	0.0	7.613562689972804	0.0
15	0.0	0.0	0.0	7.890407268012158	0.0
16	0.0	0.0	0.0	8.421194439532867	0.0
17	0.0	0.0	0.0	9.132839930283664	0.0
18	0.0	0.0	0.0	9.852231680517315	0.0
19	0.0	0.0	0.0	10.328626638712773	0.0
20	0.0	0.0	0.0	10.756186482777203	0.0
21	0.0	0.0	0.0	11.360563119385015	0.0
22	0.0	0.0	0.0	12.006028610641003	0.0
23	0.0	0.0	0.0	12.664965857519345	0.0
24	0.0	0.0	0.0	13.16224203692945	0.0
25	0.0	0.0	0.0	13.59788493436729	0.0
26	0.0	0.0	0.0	14.022582030361969	0.0
27	0.0	0.0	0.0	14.44660553857553	0.0
28	0.0	0.0	0.0	14.906834390024166	0.0
29	0.0	0.0	0.0	15.373293928448138	0.0
30	0.0	0.0	0.0	15.912669344178097	0.0
31	0.0	0.0	0.0	16.453223538525013	0.0
32	0.0	0.0	0.0	16.936185977586366	0.0
33	0.0	0.0	0.0	17.426726279185296	0.0
34	0.0	0.0	0.0	17.87567253530021	0.0
35	0.0	0.0	0.0	18.36335008882939	0.0
36	0.0	0.0	0.0	18.874098023861848	0.0
37	0.0	0.0	0.0	19.36665908880413	0.0
38	0.0	0.0	0.0	19.858546565965295	0.0
39	0.0	0.0	0.0	20.361885035405457	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCG	30	2.1637225E-6	45.000004	1
TCGTGCC	30	2.1637225E-6	45.000004	22
CGTCTCA	20	7.030529E-4	45.0	40
ACGGCCC	20	7.030529E-4	45.0	26
CGCTATT	20	7.030529E-4	45.0	35
TCCACCG	20	7.030529E-4	45.0	31
GCGATTA	40	6.8048394E-9	45.0	9
GCTAGCG	20	7.030529E-4	45.0	1
AACCTTC	20	7.030529E-4	45.0	23
CACCGGA	25	3.8885206E-5	45.0	31
CGCCGGT	20	7.030529E-4	45.0	28
CATCCGG	20	7.030529E-4	45.0	2
TACGGCT	315	0.0	44.285713	7
AGGCGAT	205	0.0	41.707317	7
CGTTATT	130	0.0	41.538464	1
ACACGAC	120	0.0	41.250004	26
GGAACGC	55	6.002665E-11	40.909092	8
CGAATAT	55	6.002665E-11	40.909092	14
CGTTTTA	105	0.0	40.714287	1
ATTACGG	50	1.0804797E-9	40.5	2
>>END_MODULE