##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936201.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407703
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980245423752095	33.0	31.0	34.0	30.0	34.0
2	32.12539274913356	33.0	31.0	34.0	30.0	34.0
3	32.06367870729428	33.0	31.0	34.0	30.0	34.0
4	35.77922899757912	37.0	35.0	37.0	35.0	37.0
5	31.192198732901154	37.0	35.0	37.0	0.0	37.0
6	33.435861399106706	37.0	35.0	37.0	17.0	37.0
7	29.607037475809598	35.0	32.0	37.0	0.0	37.0
8	32.672401233250675	35.0	35.0	37.0	17.0	37.0
9	36.57261045417865	39.0	35.0	39.0	32.0	39.0
10	36.8882225541632	38.0	37.0	39.0	33.0	39.0
11	36.97441519929949	39.0	37.0	39.0	33.0	39.0
12	36.8923113148542	39.0	35.0	39.0	33.0	39.0
13	36.61767021581887	39.0	35.0	39.0	33.0	39.0
14	37.84187263767988	40.0	37.0	41.0	33.0	41.0
15	37.99463825382693	40.0	37.0	41.0	33.0	41.0
16	38.066928622060665	40.0	37.0	41.0	33.0	41.0
17	38.01405925391768	40.0	36.0	41.0	33.0	41.0
18	37.940032327454055	40.0	36.0	41.0	33.0	41.0
19	37.83339342609694	40.0	36.0	41.0	33.0	41.0
20	37.788735918057995	40.0	36.0	41.0	33.0	41.0
21	37.69423575494907	40.0	36.0	41.0	33.0	41.0
22	37.66547952798974	39.0	36.0	41.0	33.0	41.0
23	37.60808235406656	39.0	35.0	41.0	33.0	41.0
24	37.562208274160355	39.0	35.0	41.0	33.0	41.0
25	37.41251842640354	39.0	35.0	41.0	33.0	41.0
26	37.235546463970095	39.0	35.0	41.0	32.0	41.0
27	37.180069805716414	39.0	35.0	41.0	32.0	41.0
28	37.077831166314695	39.0	35.0	41.0	32.0	41.0
29	37.07220942696031	39.0	35.0	41.0	32.0	41.0
30	36.936811845878005	39.0	35.0	41.0	31.0	41.0
31	36.79527008631283	39.0	35.0	41.0	31.0	41.0
32	36.70178536827053	39.0	35.0	41.0	31.0	41.0
33	36.57042994532785	39.0	35.0	40.0	31.0	41.0
34	36.42395567361535	39.0	35.0	40.0	30.0	41.0
35	36.30076060269363	39.0	35.0	40.0	30.0	41.0
36	36.19528185958897	39.0	35.0	40.0	30.0	41.0
37	36.09557447455623	38.0	35.0	40.0	30.0	41.0
38	35.99982585362384	38.0	35.0	40.0	29.0	41.0
39	36.02095152598828	38.0	35.0	40.0	29.0	41.0
40	35.88091821742788	38.0	35.0	40.0	29.0	41.0
41	35.78274871659026	38.0	35.0	40.0	29.0	41.0
42	35.76962641923165	38.0	35.0	40.0	29.0	41.0
43	35.62404740705857	38.0	35.0	40.0	28.0	41.0
44	35.47014616031768	38.0	34.0	40.0	27.0	41.0
45	35.33303409589824	38.0	34.0	40.0	27.0	41.0
46	35.24044954292708	38.0	34.0	40.0	27.0	41.0
47	35.08689168340679	38.0	34.0	40.0	26.0	41.0
48	34.97802812341337	37.0	34.0	40.0	26.0	41.0
49	34.87528421424419	37.0	34.0	40.0	26.0	41.0
50	34.69361765795199	37.0	34.0	40.0	25.0	41.0
51	32.788399889134986	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	12.0
10	18.0
11	13.0
12	21.0
13	9.0
14	17.0
15	34.0
16	63.0
17	108.0
18	211.0
19	371.0
20	581.0
21	888.0
22	1299.0
23	1864.0
24	2531.0
25	3315.0
26	4294.0
27	4805.0
28	5598.0
29	6876.0
30	8471.0
31	10948.0
32	14949.0
33	21970.0
34	32433.0
35	39062.0
36	40693.0
37	61728.0
38	75016.0
39	69493.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62316931688018	19.817367053958396	25.096209740914343	10.463253888247081
2	32.57616451191186	24.188686372187597	25.909301623976276	17.325847491924268
3	32.9811161556329	24.432491298813105	25.98852596130026	16.597866584253733
4	27.83938308033053	26.13274859395197	26.4368915607685	19.590976764948994
5	22.546559627964474	39.6249230444711	21.03295781487995	16.79555951268448
6	27.457487435706874	36.76082834808672	22.186248322921344	13.595435893285062
7	72.6303215821321	18.97827585276537	5.320539706600147	3.07086285850239
8	87.47102670326193	4.271001194496975	5.673737990645151	2.584234111595941
9	81.69157450398943	6.477018810261391	7.471860643654818	4.359546042094368
10	43.29377021998857	29.66865585978028	14.259644888558585	12.777929031672567
11	33.956826415307226	25.568367169238392	23.76239566547217	16.71241074998222
12	31.647302080190727	24.204874626872993	26.41923164656625	17.728591646370027
13	25.478105385538	26.98214141176297	28.65468245266775	18.88507075003127
14	21.689072682810774	29.452076634216574	28.370652165914894	20.48819851705776
15	21.365552865688993	26.261028248504424	33.21167614660672	19.16174273919986
16	24.972835618084734	23.97406935931303	31.704206248175755	19.34888877442648
17	25.294638499103517	24.05280314346473	27.53474956034172	23.11780879709004
18	26.608094617895873	24.632882269691418	27.88892895073129	20.870094161681422
19	25.895811411738446	27.106741917523298	26.37704407374976	20.620402596988495
20	28.41185863238681	25.713816184820814	26.685356742530715	19.18896844026166
21	25.854359668680388	26.941670775049488	27.680689129096425	19.523280427173702
22	26.029732427772178	24.719955457771956	27.022857325062606	22.22745478939326
23	24.017973868232513	27.48986394507767	27.41750735216567	21.07465483452415
24	23.517364355915948	24.555620145056572	30.908038449557644	21.018977049469832
25	23.137185647395285	26.872748054343482	28.142790217388637	21.847276080872597
26	22.638293071181717	28.845262360100364	26.820504141495157	21.695940427222755
27	22.778346001869007	28.001510903770637	27.83496810177997	21.38517499258038
28	20.5399518767338	28.495497948261356	29.390021657922556	21.574528517082285
29	22.519579203488817	25.792795245558654	29.612242244967536	22.075383305984992
30	24.14036688471755	26.248519142611165	28.768736065223948	20.842377907447332
31	25.309109817685915	27.03340421826673	25.878151497536194	21.77933446651116
32	26.079278298172937	26.74177035734346	26.940444392118774	20.238506952364833
33	26.259066035815287	26.255632163609295	26.06014672445383	21.425155076121587
34	23.113639095125617	26.701054444043827	28.19478885365082	21.990517607179736
35	23.207334751032	26.910275372023264	27.838156697399825	22.044233179544914
36	24.74938864810904	28.147450472525342	26.85091843817681	20.252242441188805
37	23.762886218644454	28.89529878367341	27.02947979288845	20.312335204793687
38	23.41042376435788	28.56417539238122	26.476135814551277	21.549265028709623
39	24.115839226103315	27.08442174818434	26.45038177300633	22.349357252706014
40	24.395209257719465	25.753796268362017	27.64389764117507	22.207096832743442
41	21.43594724591185	26.4368915607685	28.05473592296353	24.072425270356117
42	23.508779675400966	26.788127632124365	26.732695123656192	22.97039756881848
43	23.383443339882216	26.129314721745978	27.524202667137597	22.963039271234205
44	23.0162642904271	27.121213236105696	26.63384865944082	23.228673814026386
45	23.405518232635032	26.612754873032575	26.183520847283436	23.798206047048957
46	23.16367551869866	27.70938648967508	26.20657684638082	22.920361145245437
47	22.544842691861476	27.460185478154443	27.868570994081477	22.126400835902604
48	23.582362651243674	25.696401547204704	28.35250169854036	22.368734103011263
49	23.500440271472126	25.71528784433767	28.55485488210781	22.2294170020824
50	21.809748763192815	27.069950429601942	28.563930115795078	22.55637069141017
51	21.87057735655612	26.839880991800403	26.380477945955754	24.90906370568772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	564.0
2	942.0
3	1234.5
4	1527.0
5	1201.0
6	875.0
7	789.5
8	704.0
9	695.5
10	687.0
11	724.0
12	761.0
13	787.0
14	813.0
15	831.5
16	850.0
17	916.0
18	982.0
19	1014.0
20	1046.0
21	1253.5
22	1461.0
23	1696.5
24	1932.0
25	2201.0
26	3271.5
27	4073.0
28	5115.0
29	6157.0
30	7008.5
31	7860.0
32	8781.5
33	9703.0
34	11033.0
35	12363.0
36	13697.5
37	15032.0
38	15760.0
39	16488.0
40	18636.0
41	20784.0
42	22837.5
43	24891.0
44	27406.5
45	29922.0
46	30493.0
47	31064.0
48	30688.0
49	30312.0
50	30133.5
51	29955.0
52	28093.5
53	26232.0
54	24184.0
55	22136.0
56	21078.0
57	20020.0
58	19783.0
59	19546.0
60	18546.0
61	17546.0
62	16107.0
63	14668.0
64	12361.5
65	10055.0
66	8648.0
67	7241.0
68	6144.5
69	5048.0
70	4371.5
71	3695.0
72	3084.5
73	2474.0
74	2157.5
75	1592.0
76	1343.0
77	1092.0
78	841.0
79	709.5
80	578.0
81	433.5
82	289.0
83	216.0
84	143.0
85	109.0
86	75.0
87	63.5
88	52.0
89	36.5
90	21.0
91	15.0
92	9.0
93	7.0
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.615867569352204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.65512130660186	23.044780796326414
2	8.518033070956063	5.556460771873308
3	4.2925658041398425	4.200172734016649
4	2.7891518644467665	3.638824313664301
5	2.0553265353968295	3.351812904513924
6	1.6473598696902059	3.223804280932864
7	1.1802699338122282	2.694686950011541
8	0.9757334360010677	2.5459514025279852
9	0.7407259435424368	2.1743477351787224
>10	7.047404511677562	46.76956206162703
>50	0.08029714839436149	1.6616941404863448
>100	0.017260134701591722	1.012828156331197
>500	7.5044063919964E-4	0.12507375250972488
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.12533633551874773	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG	440	0.1079216979026399	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.056658891398885955	0.0
2	0.0	0.0	0.0	0.2401257778333738	0.0
3	0.0	0.0	0.0	0.33234977422290246	0.0
4	0.0	0.0	0.0	0.4542522375356571	0.0
5	0.0	0.0	0.0	0.8753921359420951	0.0
6	0.0	0.0	0.0	1.1022729781237812	0.0
7	0.0	0.0	0.0	1.3166447144122069	0.0
8	0.0	0.0	0.0	1.75593508019318	0.0
9	0.0	0.0	0.0	1.9126668187381501	0.0
10	0.0	0.0	0.0	2.460124159007905	0.0
11	0.0	0.0	0.0	3.0348072003394626	0.0
12	0.0	0.0	0.0	3.6109619011878746	0.0
13	0.0	0.0	0.0	3.8091453827909043	0.0
14	0.0	0.0	0.0	3.8925394220793077	2.4527658614236343E-4
15	0.0	0.0	0.0	4.0551577986916945	2.4527658614236343E-4
16	0.0	0.0	0.0	4.391431998292875	2.4527658614236343E-4
17	0.0	0.0	0.0	4.822873513317292	2.4527658614236343E-4
18	0.0	0.0	0.0	5.2462209009990115	2.4527658614236343E-4
19	0.0	0.0	0.0	5.526326762373591	2.4527658614236343E-4
20	0.0	0.0	0.0	5.783376624650788	2.4527658614236343E-4
21	0.0	0.0	0.0	6.113273633012266	2.4527658614236343E-4
22	0.0	0.0	0.0	6.4657360872988425	2.4527658614236343E-4
23	0.0	0.0	0.0	6.820651307446842	2.4527658614236343E-4
24	4.905531722847269E-4	0.0	0.0	7.0992855093045675	2.4527658614236343E-4
25	4.905531722847269E-4	0.0	0.0	7.34971290375592	2.4527658614236343E-4
26	7.358297584270904E-4	0.0	0.0	7.605291106516263	2.4527658614236343E-4
27	7.358297584270904E-4	0.0	0.0	7.846888543866491	2.4527658614236343E-4
28	7.358297584270904E-4	0.0	0.0	8.109825044211105	2.4527658614236343E-4
29	7.358297584270904E-4	0.0	0.0	8.400232522203662	2.4527658614236343E-4
30	7.358297584270904E-4	0.0	0.0	8.71222433977675	2.4527658614236343E-4
31	7.358297584270904E-4	0.0	0.0	9.021027561729985	2.4527658614236343E-4
32	7.358297584270904E-4	0.0	0.0	9.320264996823669	2.4527658614236343E-4
33	7.358297584270904E-4	0.0	0.0	9.581484561065285	2.4527658614236343E-4
34	7.358297584270904E-4	0.0	0.0	9.883174762020392	2.4527658614236343E-4
35	7.358297584270904E-4	0.0	0.0	10.179223601494225	2.4527658614236343E-4
36	7.358297584270904E-4	0.0	0.0	10.493668184928735	2.4527658614236343E-4
37	7.358297584270904E-4	0.0	0.0	10.788245364885713	2.4527658614236343E-4
38	7.358297584270904E-4	0.0	0.0	11.100482459044942	2.4527658614236343E-4
39	7.358297584270904E-4	0.0	0.0	11.434794445956983	2.4527658614236343E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	30	2.1620435E-6	45.000004	5
AACCGCT	20	7.0279255E-4	45.0	36
TTAGCGT	20	7.0279255E-4	45.0	43
CGCATCG	25	3.8863625E-5	45.0	21
TAAACGG	20	7.0279255E-4	45.0	2
TTCGTCA	20	7.0279255E-4	45.0	23
TAACGCC	25	3.8863625E-5	45.0	12
TCGGCAT	25	3.8863625E-5	45.0	32
TACGAAT	40	6.7957444E-9	45.0	12
GTCCGTA	35	1.2093551E-7	45.0	26
GGACGTC	20	7.0279255E-4	45.0	11
GGACGTA	20	7.0279255E-4	45.0	8
CATACGG	35	1.2093551E-7	45.0	2
CGGCTTA	20	7.0279255E-4	45.0	39
CCGCGTA	20	7.0279255E-4	45.0	33
CGACTTG	20	7.0279255E-4	45.0	2
CAGTACG	25	3.8863625E-5	45.0	1
ATACGAC	20	7.0279255E-4	45.0	44
TAGTGCG	25	3.8863625E-5	45.0	1
TATACGG	20	7.0279255E-4	45.0	2
>>END_MODULE