##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	749567
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93166321356196	31.0	31.0	34.0	30.0	34.0
2	32.056369877542636	33.0	31.0	34.0	30.0	34.0
3	31.95067285512836	33.0	31.0	34.0	30.0	34.0
4	35.695200028816636	37.0	35.0	37.0	35.0	37.0
5	31.17513311018228	37.0	35.0	37.0	0.0	37.0
6	33.39947596412329	37.0	35.0	37.0	17.0	37.0
7	29.558271642161408	35.0	32.0	37.0	0.0	37.0
8	32.593672080014194	35.0	33.0	37.0	17.0	37.0
9	36.52440942570844	39.0	35.0	39.0	32.0	39.0
10	36.82944019680696	38.0	37.0	39.0	33.0	39.0
11	36.921082438261024	39.0	37.0	39.0	33.0	39.0
12	36.985060708382306	39.0	37.0	39.0	33.0	39.0
13	36.84311475825376	39.0	37.0	39.0	33.0	39.0
14	38.168830804984744	40.0	37.0	41.0	33.0	41.0
15	38.24338985040697	40.0	37.0	41.0	33.0	41.0
16	38.27465856954748	40.0	37.0	41.0	34.0	41.0
17	38.23960233041209	40.0	37.0	41.0	34.0	41.0
18	38.131825440554344	40.0	37.0	41.0	33.0	41.0
19	38.022144784922496	40.0	37.0	41.0	33.0	41.0
20	37.99226086527288	40.0	37.0	41.0	33.0	41.0
21	37.93549342487063	40.0	37.0	41.0	33.0	41.0
22	37.923118280287156	40.0	37.0	41.0	33.0	41.0
23	37.8766367783	40.0	36.0	41.0	33.0	41.0
24	37.81527735345873	40.0	36.0	41.0	33.0	41.0
25	37.6931855324474	40.0	36.0	41.0	33.0	41.0
26	37.527724673044574	40.0	36.0	41.0	33.0	41.0
27	37.49098212701466	40.0	36.0	41.0	33.0	41.0
28	37.39555103146216	39.0	36.0	41.0	32.0	41.0
29	37.3807224704396	40.0	36.0	41.0	32.0	41.0
30	37.288183711396044	39.0	36.0	41.0	32.0	41.0
31	37.16319955387577	39.0	35.0	41.0	32.0	41.0
32	37.06918794450663	39.0	35.0	41.0	31.0	41.0
33	36.92851206096319	39.0	35.0	41.0	31.0	41.0
34	36.80733410088758	39.0	35.0	41.0	31.0	41.0
35	36.686553703671585	39.0	35.0	41.0	30.0	41.0
36	36.579481220491296	39.0	35.0	41.0	30.0	41.0
37	36.47912061230017	39.0	35.0	41.0	30.0	41.0
38	36.38607355980186	39.0	35.0	41.0	30.0	41.0
39	36.37367440135438	39.0	35.0	41.0	30.0	41.0
40	36.249386645890226	39.0	35.0	40.0	30.0	41.0
41	36.13333431167594	39.0	35.0	40.0	29.0	41.0
42	36.105372835250215	39.0	35.0	40.0	29.0	41.0
43	35.93472898353316	39.0	35.0	40.0	28.0	41.0
44	35.794105130028406	38.0	35.0	40.0	28.0	41.0
45	35.6903278826309	38.0	35.0	40.0	27.0	41.0
46	35.55496840175728	38.0	34.0	40.0	27.0	41.0
47	35.420189255930424	38.0	34.0	40.0	26.0	41.0
48	35.31050593209146	38.0	34.0	40.0	26.0	41.0
49	35.17729035563198	38.0	34.0	40.0	26.0	41.0
50	35.01521411695018	38.0	34.0	40.0	26.0	41.0
51	33.06303105659668	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	22.0
10	45.0
11	31.0
12	14.0
13	19.0
14	24.0
15	46.0
16	83.0
17	160.0
18	304.0
19	562.0
20	944.0
21	1435.0
22	2171.0
23	3130.0
24	4637.0
25	6138.0
26	7580.0
27	8905.0
28	10061.0
29	12256.0
30	15066.0
31	19050.0
32	25092.0
33	34888.0
34	50482.0
35	61564.0
36	73385.0
37	116355.0
38	149553.0
39	145535.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.676816081817904	19.004171741818944	25.189876288577274	12.129135887785882
2	30.566046797684532	25.943911618307634	26.55092873619036	16.939112847817473
3	32.12828206151018	26.226341341067577	26.25996075067339	15.385415846748856
4	27.453049560612996	26.49983257000375	28.418273483224315	17.628844386158942
5	22.847724086039005	39.97934807695643	21.106985766449164	16.0659420705554
6	26.45527351124049	36.89263267993388	23.226742906237867	13.425350902587759
7	71.48660493324813	18.871428438018214	6.409300302708097	3.2326663260255586
8	86.19683097041359	4.90776675067072	6.245205565346393	2.6501967135693008
9	80.75102025569429	6.910389598261396	7.254721726009816	5.0838684200345
10	40.111824560046	33.44344134680422	14.400580601867476	12.0441534912823
11	29.297180905776266	26.91687334154252	26.866711047844955	16.91923470483626
12	28.5200655845308	23.70848770023227	29.167639450509427	18.603807264727504
13	24.075366178073473	24.366067342879287	31.052460954124182	20.506105524923054
14	20.622305944632036	28.620256761570346	28.976329000609685	21.781108293187934
15	19.68456455526991	27.7987157919172	33.19596513720588	19.32075451560701
16	22.918965215917993	26.224340185733897	31.654942119917234	19.20175247843088
17	23.39790839244524	24.963212094449194	29.233410755809686	22.40546875729588
18	22.999945301754213	27.38967964171315	28.608516650279427	21.00185840625321
19	24.81552683082366	28.43361567411586	26.710754342173548	20.040103152886935
20	26.95035934079275	26.836426897128607	27.824730811255034	18.388482950823608
21	25.643071266477847	26.6012244402435	28.189741544118135	19.565962749160516
22	23.71195636947731	25.768877231788483	27.668373874516888	22.850792524217315
23	23.094533243859455	27.912381414870186	28.72378319749936	20.269302143771004
24	23.32800136612204	25.597978566292273	28.977929924876626	22.09609014270906
25	22.223096801219906	28.264718163953322	27.58512581263583	21.927059222190945
26	22.179471614945694	29.86270740307404	26.354815513489786	21.603005468490473
27	22.74379741904326	29.117477156811866	27.718135937147714	20.42058948699716
28	21.031075274124927	29.310121710267396	29.51837527532562	20.140427740282057
29	23.1504321828469	27.248931716577708	28.436950933005324	21.163685167570076
30	22.340097683062353	26.81948378197012	28.41080250331191	22.42961603165561
31	22.16933242792172	27.839806181435417	28.082346207877347	21.908515182765516
32	23.805210208026768	29.179379561800346	27.07603189574781	19.939378334425076
33	22.914696084539475	28.010037795153735	27.22051531083946	21.854750809467333
34	22.853460731328887	27.655299659670185	28.108361227215177	21.38287838178575
35	21.915452521255606	26.296782008813086	28.320083461518454	23.467682008412858
36	23.42405682213865	27.9166505462487	27.770032565467794	20.889260066144853
37	22.163996013698576	28.94110866673693	28.588638507298214	20.306256812266284
38	22.2218961080197	27.322841053568258	27.996029707817982	22.459233130594065
39	22.631199078934905	27.186362259811332	27.948802441943148	22.233636219310615
40	23.11054248652889	25.882943085808208	30.684648603793924	20.321865823868983
41	20.950228598644284	26.94969228901486	29.805207539819655	22.294871572521203
42	22.421344589609735	27.5278927700926	28.32381895147465	21.726943688823013
43	23.58628381452225	27.579389167345948	27.204105837103288	21.630221181028514
44	22.453496485304182	27.771766900090316	27.621680250064372	22.15305636454113
45	21.739217441536248	26.634977260204888	27.405555474027004	24.220249824231857
46	22.162128268720473	28.483244326391105	26.81708239556971	22.537545009318713
47	21.799385511902205	27.17528920029831	29.645915575258787	21.379409712540706
48	22.90922625996075	26.180181358037373	28.114898334638532	22.795694047363344
49	21.256538775052796	26.890724911849105	29.275435017816953	22.577301295281142
50	20.387370308458085	26.507303549916152	29.08052248831659	24.024803653309178
51	20.437265781444488	26.39990821367536	28.307809708805216	24.855016296074933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	237.0
1	703.5
2	1170.0
3	2952.5
4	4735.0
5	3641.0
6	2547.0
7	2096.0
8	1645.0
9	1662.5
10	1680.0
11	1605.0
12	1530.0
13	1545.0
14	1560.0
15	1707.0
16	1854.0
17	1890.0
18	1926.0
19	2129.0
20	2332.0
21	2731.5
22	3131.0
23	3638.5
24	4146.0
25	5060.0
26	7615.0
27	9256.0
28	11054.5
29	12853.0
30	14872.5
31	16892.0
32	19875.5
33	22859.0
34	25535.0
35	28211.0
36	29890.5
37	31570.0
38	32811.5
39	34053.0
40	38035.0
41	42017.0
42	43836.0
43	45655.0
44	49130.5
45	52606.0
46	57846.5
47	63087.0
48	59299.0
49	55511.0
50	52480.5
51	49450.0
52	46068.0
53	42686.0
54	39863.5
55	37041.0
56	35224.5
57	33408.0
58	33086.5
59	32765.0
60	32781.0
61	32797.0
62	29107.5
63	25418.0
64	21107.5
65	16797.0
66	13687.5
67	10578.0
68	8500.0
69	6422.0
70	5599.0
71	4776.0
72	3988.0
73	3200.0
74	2679.0
75	1862.5
76	1567.0
77	1138.5
78	710.0
79	532.5
80	355.0
81	276.5
82	198.0
83	157.5
84	117.0
85	83.0
86	49.0
87	33.0
88	17.0
89	13.0
90	9.0
91	8.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	749567.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.410764625202763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44956461912308	22.75696221449693
2	8.023041708455962	5.040197493649897
3	3.6676711495432994	3.456130656028543
4	2.4184724200070415	3.0386427174954282
5	1.8276749733694104	2.8704334199940136
6	1.4347897514857881	2.7040705902343953
7	1.1162640156743882	2.45438943791523
8	0.8557266030018529	2.1503221528332417
9	0.7346326513285888	2.076783596718386
>10	7.297683064773279	47.67171455017558
>50	0.14400042558913748	2.742063795215615
>100	0.02782830307005304	1.7487715446871233
>500	8.834381927000965E-4	0.16677430948222166
>1k	0.001766876385400193	1.1227435210733259
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2095	0.2794946949372104	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	2060	0.2748253324919587	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	1976	0.26361886262335454	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	1961	0.26161770728967526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04869477978619657	0.0
2	0.0	0.0	0.0	0.22359575594976833	0.0
3	0.0	0.0	0.0	0.2973716825847456	0.0
4	0.0	0.0	0.0	0.4769420211935691	0.0
5	0.0	0.0	0.0	1.1313198153067037	0.0
6	0.0	0.0	0.0	1.406278558154241	0.0
7	0.0	0.0	0.0	1.7019159061164646	0.0
8	0.0	0.0	0.0	2.140435744903391	0.0
9	0.0	0.0	0.0	2.30879961364361	0.0
10	0.0	0.0	0.0	3.0877826798671766	0.0
11	0.0	0.0	0.0	3.562856956082645	0.0
12	0.0	0.0	0.0	4.315691592612802	0.0
13	0.0	0.0	0.0	4.49379441731026	0.0
14	0.0	0.0	0.0	4.583846407325829	0.0
15	0.0	0.0	0.0	4.744205654731331	0.0
16	0.0	0.0	0.0	5.015295497267089	0.0
17	0.0	0.0	0.0	5.344152023768389	0.0
18	0.0	0.0	0.0	5.688484151516809	0.0
19	0.0	0.0	0.0	5.924753891246547	0.0
20	0.0	0.0	0.0	6.137676818750025	0.0
21	0.0	0.0	0.0	6.435848963468242	0.0
22	0.0	0.0	0.0	6.76590618316975	0.0
23	0.0	0.0	0.0	7.08995993687022	0.0
24	0.0	0.0	0.0	7.342772560691706	0.0
25	0.0	0.0	0.0	7.572238372286933	0.0
26	0.0	0.0	0.0	7.789830662235664	0.0
27	0.0	0.0	0.0	8.00195312760567	0.0
28	0.0	0.0	0.0	8.23608830164615	0.0
29	0.0	0.0	0.0	8.485032025155856	0.0
30	0.0	0.0	0.0	8.769729723960634	0.0
31	0.0	0.0	0.0	9.039352052585025	0.0
32	0.0	0.0	0.0	9.30390478769743	0.0
33	1.3341035557862073E-4	0.0	0.0	9.556183770096602	0.0
34	1.3341035557862073E-4	0.0	0.0	9.806728417873252	0.0
35	1.3341035557862073E-4	0.0	0.0	10.085289240321412	0.0
36	1.3341035557862073E-4	0.0	0.0	10.37465630157144	0.0
37	1.3341035557862073E-4	0.0	0.0	10.679899195135325	0.0
38	1.3341035557862073E-4	0.0	0.0	10.966331228562623	0.0
39	1.3341035557862073E-4	0.0	0.0	11.284514926617634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	20	7.0317136E-4	45.000004	28
GTCGATC	20	7.0317136E-4	45.000004	14
TAAGTCG	20	7.0317136E-4	45.000004	27
TAACGAG	30	2.1644864E-6	45.000004	1
ATCGTAG	20	7.0317136E-4	45.000004	1
TACGAGT	25	3.889502E-5	45.000004	23
CGTTTAG	20	7.0317136E-4	45.000004	2
TGGTCGA	20	7.0317136E-4	45.000004	12
TGCGTAG	30	2.1644864E-6	45.000004	1
AGTCGAA	20	7.0317136E-4	45.000004	23
GTTTACG	55	1.8189894E-12	45.0	1
CGTTATT	160	0.0	43.593754	1
CGAATAT	100	0.0	42.750004	14
AGGTACG	50	1.0804797E-9	40.500004	1
GTATGCG	45	1.9268555E-8	40.000004	1
TACGGCT	250	0.0	39.600002	7
GACACGA	80	0.0	39.375004	25
TATCCGG	40	3.45608E-7	39.375004	2
GGCCGAT	145	0.0	38.793106	8
CGGTCTA	35	6.24538E-6	38.57143	31
>>END_MODULE