FastQCFastQC Report
Sat 14 Jan 2017
SRR2936198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026305
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120991.1788893165287122No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36860.3591524936544205No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23730.23121781536677694No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.16135554245570274No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA253.890513E-545.014
CGTTATT12100.043.5123981
CGGTTTT13700.042.700731
CGTTTTT52450.042.5119171
CGTTTTA6900.042.3913041
TTGTACG501.0804797E-940.51
CACGACC1850.040.13513627
TCTACGG451.9279469E-840.02
TCTAGCG403.4577351E-739.3750041
AATACGG750.039.02
ATAGCGG1750.038.5714262
GTCTACG356.2476356E-638.5714261
GAACGCC356.2476356E-638.57142640
GCCGATA659.094947E-1238.0769279
GCGATAT659.094947E-1238.0769279
CCGTCAA301.1398365E-437.50000424
TAATGCG301.1398365E-437.5000041
ACGACCA2000.037.12528
ATAACGG850.037.0588232
TACGGGA4450.036.9101144
Produced by FastQC (version 0.11.5)