##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936198.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1026305
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8392271303365	31.0	31.0	34.0	30.0	34.0
2	31.93932895191975	33.0	31.0	34.0	30.0	34.0
3	31.403642192135866	33.0	31.0	34.0	28.0	34.0
4	35.38889024217947	37.0	35.0	37.0	33.0	37.0
5	30.950286708142315	37.0	35.0	37.0	0.0	37.0
6	33.30787339046385	37.0	35.0	37.0	17.0	37.0
7	29.790542772372735	35.0	32.0	37.0	0.0	37.0
8	32.84357379141678	35.0	35.0	37.0	17.0	37.0
9	36.72986977555405	39.0	35.0	39.0	32.0	39.0
10	37.00405240157653	39.0	37.0	39.0	34.0	39.0
11	37.071199107477796	39.0	37.0	39.0	34.0	39.0
12	37.166849036105255	39.0	37.0	39.0	34.0	39.0
13	37.090127203901375	39.0	37.0	39.0	33.0	39.0
14	38.37840602939672	40.0	38.0	41.0	34.0	41.0
15	38.44816599353993	40.0	38.0	41.0	34.0	41.0
16	38.47375780104355	40.0	38.0	41.0	34.0	41.0
17	38.43294537198981	40.0	38.0	41.0	34.0	41.0
18	38.28281261418389	40.0	38.0	41.0	34.0	41.0
19	38.115450085500896	40.0	37.0	41.0	34.0	41.0
20	37.923779967943254	40.0	36.0	41.0	34.0	41.0
21	37.80923702018406	40.0	36.0	41.0	33.0	41.0
22	37.76441603616858	39.0	35.0	41.0	33.0	41.0
23	37.684922123540275	39.0	35.0	41.0	33.0	41.0
24	37.60213191984839	39.0	35.0	41.0	33.0	41.0
25	37.459569036495	39.0	35.0	41.0	33.0	41.0
26	37.32741241638694	39.0	35.0	41.0	33.0	41.0
27	37.2996428936817	39.0	35.0	41.0	33.0	41.0
28	37.179370654922266	39.0	35.0	41.0	33.0	41.0
29	37.14296529784031	39.0	35.0	41.0	32.0	41.0
30	36.96773376335495	39.0	35.0	41.0	32.0	41.0
31	36.79244863856261	39.0	35.0	41.0	31.0	41.0
32	36.566328722942984	39.0	35.0	41.0	30.0	41.0
33	36.25149541315691	39.0	35.0	41.0	30.0	41.0
34	35.9176706729481	39.0	35.0	41.0	27.0	41.0
35	35.642009928822326	39.0	35.0	41.0	25.0	41.0
36	35.419525384754046	39.0	35.0	40.0	24.0	41.0
37	35.23584801788942	39.0	35.0	40.0	23.0	41.0
38	35.062263167382014	39.0	34.0	40.0	23.0	41.0
39	34.97445983406492	39.0	34.0	40.0	22.0	41.0
40	34.79859690832647	38.0	34.0	40.0	21.0	41.0
41	34.64084458323793	38.0	34.0	40.0	20.0	41.0
42	34.522403184238605	38.0	34.0	40.0	19.0	41.0
43	34.347537038209886	38.0	34.0	40.0	18.0	41.0
44	34.087859846731725	38.0	33.0	40.0	17.0	41.0
45	33.96649144260235	38.0	33.0	40.0	17.0	41.0
46	33.867682608971016	38.0	33.0	40.0	17.0	41.0
47	33.73940690145717	38.0	33.0	40.0	15.0	41.0
48	33.64177510584086	38.0	33.0	40.0	15.0	41.0
49	33.511746508104316	37.0	33.0	40.0	15.0	41.0
50	33.34241672797073	37.0	33.0	40.0	15.0	41.0
51	31.642689064167087	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	33.0
10	44.0
11	40.0
12	17.0
13	25.0
14	34.0
15	44.0
16	97.0
17	242.0
18	517.0
19	908.0
20	1663.0
21	2715.0
22	4462.0
23	7102.0
24	11427.0
25	16878.0
26	21130.0
27	22179.0
28	21380.0
29	21014.0
30	23490.0
31	27237.0
32	34211.0
33	45852.0
34	63949.0
35	77881.0
36	92882.0
37	147284.0
38	190016.0
39	191505.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75612025664885	19.154344955934153	23.97659565139018	18.112939136026814
2	37.465178480081455	22.04880615411598	24.65543868538105	15.830576680421512
3	31.141424820107083	22.76779319987723	30.554951987956798	15.535829992058892
4	25.200793136543233	25.219306151680055	32.07993725062238	17.499963461154337
5	20.095293309493766	38.50278426003966	26.737178519056226	14.66474391141035
6	24.322009539074642	33.45155679841763	29.252610091541985	12.973823570965745
7	68.69030161599134	17.480670950643326	11.048762307501182	2.7802651258641435
8	83.79945532760729	2.610042823527119	11.634260770433741	1.9562410784318502
9	78.34493644676778	5.385436103302625	13.190133537301291	3.0794939126283123
10	36.0789433940203	34.520342393343114	18.780187176326727	10.62052703630987
11	23.978934137512727	25.069350729071765	34.97722411953561	15.974491013879891
12	22.466323363912288	23.77451147563346	35.823853532819186	17.93531162763506
13	21.440312577645045	23.531601229653955	37.056917777853556	17.971168414847437
14	19.583262285577874	25.750239938419867	35.79218653324304	18.874311242759216
15	19.02368204383687	25.986134726031736	36.7966637597985	18.193519470332895
16	23.600976317956164	25.397031096993583	34.24021124324641	16.76178134180385
17	22.921353788591112	24.852261267362042	33.83857625169905	18.38780869234779
18	23.041298639293387	24.21278274976737	34.45437759730295	18.291541013636298
19	22.111165784050552	26.386892785283127	32.89840739351362	18.6035340371527
20	22.94814894207862	26.790184204500612	32.660368993622754	17.601297859798013
21	23.260531713282116	25.936149585162305	33.98521881896707	16.81809988258851
22	22.90449720112442	23.424615489547453	34.25736014147841	19.413527167849715
23	21.105811625199138	25.60993077106708	34.33735585425385	18.94690174947993
24	20.63314511767944	25.358153765206247	35.153487511022554	18.855213606091755
25	21.110293723600684	27.08200778521005	33.280457563784644	18.52724092740462
26	20.703104827512288	26.903698218365886	33.12913802427154	19.26405892985029
27	20.65721203735732	26.206926790768826	33.68287205070617	19.452989121167686
28	19.25499729612542	26.709408996350987	34.07680952543347	19.95878418209012
29	20.37074748734538	26.22933728277656	33.587091556603546	19.81282367327451
30	21.27924934595466	25.26461432030439	34.35430987864231	19.10182645509863
31	21.378342695397567	26.41914440638991	33.062686043622506	19.13982685459001
32	21.820706320245932	26.40618529579414	32.87248917232207	18.900619211637864
33	21.50033372145707	26.630387652793274	32.32226287507125	19.547015750678405
34	20.014225790578823	27.630187907103636	33.401961405235284	18.953624897082253
35	20.918050676943015	27.874364833066196	32.073019229176516	19.13456526081428
36	21.120524600386826	28.84015960167786	30.479730684348223	19.55958511358709
37	21.294546942672987	28.54911551634261	30.365339738186993	19.790997802797413
38	21.632555624302718	27.791056264950477	30.66710188491725	19.909286225829554
39	21.535703324060588	27.49981730577168	29.964776552779142	20.999702817388595
40	22.707772056065203	26.37120544087771	30.75109251148538	20.169929991571706
41	20.595534465875154	27.104710587983107	30.857980814670107	21.44177413147164
42	21.005061848086097	27.86510832549778	30.341565129274432	20.78826469714169
43	20.898270981823142	27.02890466284389	30.85622695007819	21.216597405254774
44	21.11156040358373	27.79690248025684	30.655604328148065	20.43593278801136
45	20.97173842083981	27.321312865083968	30.207102177228016	21.499846536848207
46	20.93860986743707	27.43619099585406	30.063967339143822	21.56123179756505
47	20.5978729519977	27.812492387740484	30.664276214185843	20.925358446075972
48	20.631488690009306	27.382210941191946	30.812282898358674	21.174017470440074
49	21.700761469543654	27.034263693541394	30.704615099799764	20.560359737115185
50	20.50355401172166	27.35882607996648	30.897832515675166	21.239787392636693
51	20.47822041206074	26.608951530003267	30.573854750780715	22.33897330715528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	359.0
1	725.0
2	1091.0
3	13546.5
4	26002.0
5	20065.5
6	14129.0
7	11372.0
8	8615.0
9	8175.5
10	7736.0
11	7630.0
12	7524.0
13	7238.0
14	6952.0
15	6771.0
16	6590.0
17	6419.5
18	6249.0
19	6018.0
20	5787.0
21	6038.0
22	6289.0
23	6802.0
24	7315.0
25	8585.5
26	11893.5
27	13931.0
28	16259.5
29	18588.0
30	21141.5
31	23695.0
32	27225.0
33	30755.0
34	34678.5
35	38602.0
36	41131.0
37	43660.0
38	46934.5
39	50209.0
40	53403.0
41	56597.0
42	59164.5
43	61732.0
44	64182.0
45	66632.0
46	69531.0
47	72430.0
48	72914.0
49	73398.0
50	70585.0
51	67772.0
52	63340.0
53	58908.0
54	54398.0
55	49888.0
56	45891.5
57	41895.0
58	39177.5
59	36460.0
60	32462.5
61	28465.0
62	24681.5
63	20898.0
64	18579.0
65	16260.0
66	14264.0
67	12268.0
68	10630.5
69	8993.0
70	7630.0
71	6267.0
72	5477.5
73	4688.0
74	4111.0
75	3085.0
76	2636.0
77	1931.5
78	1227.0
79	926.0
80	625.0
81	519.0
82	413.0
83	300.0
84	187.0
85	140.5
86	94.0
87	73.0
88	52.0
89	41.0
90	30.0
91	22.0
92	14.0
93	10.0
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1026305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.40679655852879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09046734763648	27.687639132299367
2	9.081467929184718	6.9758018241800235
3	4.5092124733266035	5.195532183067058
4	2.8138789057142866	4.322882986884169
5	1.9933272562276863	3.8278657202253568
6	1.4130192515956173	3.256172575759049
7	1.1245025398655915	3.0231978194716675
8	0.8915379256014535	2.739289258623024
9	0.7150142380965513	2.471526574112314
>10	5.305212098958966	36.52208379009051
>50	0.04824635729076838	1.203739424575544
>100	0.013087227305694019	0.8214689599933723
>500	0.0	0.0
>1k	7.698368966571129E-4	0.7603638879980693
>5k	0.0	0.0
>10k+	2.566122988857043E-4	1.1924358627204978
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12099	1.1788893165287122	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3686	0.3591524936544205	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2373	0.23121781536677694	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1656	0.16135554245570274	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.743692177276736E-5	0.0	0.0	0.03429779646401411	0.0
2	9.743692177276736E-5	0.0	0.0	0.09889847559935887	0.0
3	9.743692177276736E-5	0.0	0.0	0.1844480929158486	0.0
4	9.743692177276736E-5	0.0	0.0	0.2745772455556584	0.0
5	9.743692177276736E-5	0.0	0.0	0.5493493649548623	0.0
6	9.743692177276736E-5	0.0	0.0	0.9961950882047734	0.0
7	9.743692177276736E-5	0.0	0.0	1.3438500250900074	0.0
8	9.743692177276736E-5	0.0	0.0	1.9236971465597459	0.0
9	9.743692177276736E-5	0.0	0.0	2.293372827765625	0.0
10	9.743692177276736E-5	0.0	0.0	2.7380749387365353	0.0
11	9.743692177276736E-5	0.0	0.0	3.1772231451663977	0.0
12	9.743692177276736E-5	0.0	0.0	3.5053906976970786	0.0
13	9.743692177276736E-5	0.0	0.0	3.7097159226545715	0.0
14	9.743692177276736E-5	0.0	0.0	3.8433993793268084	0.0
15	9.743692177276736E-5	0.0	0.0	3.9651955315427676	0.0
16	9.743692177276736E-5	0.0	0.0	4.153443664407754	0.0
17	9.743692177276736E-5	0.0	0.0	4.386610218209986	0.0
18	9.743692177276736E-5	0.0	0.0	4.641018020958682	0.0
19	9.743692177276736E-5	0.0	0.0	4.8281943476841676	0.0
20	1.9487384354553472E-4	0.0	0.0	5.011083449851652	0.0
21	1.9487384354553472E-4	0.0	0.0	5.219695899367148	0.0
22	1.9487384354553472E-4	0.0	0.0	5.456370182353199	0.0
23	1.9487384354553472E-4	0.0	0.0	5.7116549173978495	0.0
24	1.9487384354553472E-4	0.0	0.0	5.918903250008526	0.0
25	1.9487384354553472E-4	0.0	0.0	6.101207730645373	0.0
26	1.9487384354553472E-4	0.0	0.0	6.301050857201319	0.0
27	1.9487384354553472E-4	0.0	0.0	6.5035247806451295	0.0
28	2.9231076531830206E-4	0.0	0.0	6.731916925280497	0.0
29	2.9231076531830206E-4	0.0	0.0	6.965862974456911	0.0
30	2.9231076531830206E-4	0.0	0.0	7.261389158193714	0.0
31	2.9231076531830206E-4	0.0	0.0	7.520961117796366	0.0
32	2.9231076531830206E-4	0.0	0.0	7.797097354100389	0.0
33	2.9231076531830206E-4	0.0	0.0	8.051602593770857	0.0
34	2.9231076531830206E-4	0.0	0.0	8.300748802743824	0.0
35	2.9231076531830206E-4	0.0	0.0	8.564997734591568	0.0
36	2.9231076531830206E-4	0.0	0.0	8.825738937255494	0.0
37	2.9231076531830206E-4	0.0	0.0	9.09953668743697	0.0
38	2.9231076531830206E-4	0.0	0.0	9.391847452755272	0.0
39	2.9231076531830206E-4	0.0	0.0	9.808000545646761	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	25	3.890513E-5	45.0	14
CGTTATT	1210	0.0	43.512398	1
CGGTTTT	1370	0.0	42.70073	1
CGTTTTT	5245	0.0	42.511917	1
CGTTTTA	690	0.0	42.391304	1
TTGTACG	50	1.0804797E-9	40.5	1
CACGACC	185	0.0	40.135136	27
TCTACGG	45	1.9279469E-8	40.0	2
TCTAGCG	40	3.4577351E-7	39.375004	1
AATACGG	75	0.0	39.0	2
ATAGCGG	175	0.0	38.571426	2
GTCTACG	35	6.2476356E-6	38.571426	1
GAACGCC	35	6.2476356E-6	38.571426	40
GCCGATA	65	9.094947E-12	38.076927	9
GCGATAT	65	9.094947E-12	38.076927	9
CCGTCAA	30	1.1398365E-4	37.500004	24
TAATGCG	30	1.1398365E-4	37.500004	1
ACGACCA	200	0.0	37.125	28
ATAACGG	85	0.0	37.058823	2
TACGGGA	445	0.0	36.910114	4
>>END_MODULE