FastQCFastQC Report
Sat 14 Jan 2017
SRR2936197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48518
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15453.184385176635476No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC12302.535141596933097No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG12242.522775052557814No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8631.7787212993115957No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG8371.7251329403520341No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC7741.5952842244115588No Hit
GAATCTATCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC7641.5746733171194196No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC5451.1232944474215756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4390.904818830124902No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.6780988499113731No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT3180.6554268518900201No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT2830.5832886763675337No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC2740.5647388598046086No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC2680.5523723154293252No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG2670.5503112247001113No Hit
GAATGAATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT2320.47817304917762476No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC2250.46374541407312747No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.3627519683416464No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC1710.35244651469557686No Hit
GCTGTCACTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC1680.3462632425079352No Hit
GAACTGACTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT1610.3318356074034379No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTT1520.31328579084051283No Hit
GAATCTATCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTT1430.2947359742775877No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT1220.2514530689640958No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA1190.2452697967764541No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC990.20404798219217612No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.1958036192753205No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC940.1937425285461066No Hit
GAATGCAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT770.1587039861494703No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.144276351044973No Hit
CCTGTCACTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC680.1401541695865452No Hit
GAATAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT650.1339708973989035No Hit
GCTGACTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC640.1319098066696896No Hit
GAATGAAACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA630.1298487159404757No Hit
GAATTATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG600.12366544375283399No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.1174821715651923No Hit
GAACAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT560.11542108083597839No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT540.1112988993775506No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.10511562718990891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTCA302.1226515E-645.0000041
GAATATG302.1226515E-645.0000041
AACGGCT600.045.0000047
AAACGGC750.045.0000046
TGAAACG750.045.0000044
AATCTAT850.045.02
CGTTATT850.045.01
AACTGAC351.1800694E-745.02
ATCTATC850.045.03
ATGAAAC1600.045.03
AAGGGGC253.8356324E-545.05
TACAAGG206.966592E-445.02
TATCTCT850.045.06
GAACTGA351.1800694E-745.01
AAAGGGT206.966592E-445.04
GAATCTG1850.045.01
GAATCTA900.045.01
ATATGTC253.8356324E-545.03
TTAGGAG206.966592E-445.05
CACTAGG206.966592E-445.03
Produced by FastQC (version 0.11.5)