##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48518
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118430273300632	31.0	30.0	33.0	28.0	34.0
2	31.180922544210397	31.0	31.0	34.0	28.0	34.0
3	30.346551795210026	31.0	30.0	34.0	26.0	34.0
4	34.67234840677687	35.0	35.0	37.0	32.0	37.0
5	31.21942371903211	35.0	33.0	37.0	0.0	37.0
6	33.19753493548786	35.0	35.0	37.0	17.0	37.0
7	21.36984212045014	32.0	0.0	35.0	0.0	37.0
8	27.905189826456162	33.0	17.0	35.0	17.0	37.0
9	33.86510161177295	35.0	32.0	39.0	28.0	39.0
10	35.75703862484026	37.0	34.0	39.0	32.0	39.0
11	36.223690176841586	37.0	35.0	39.0	32.0	39.0
12	36.55241353724391	38.0	35.0	39.0	32.0	39.0
13	36.34265633373181	38.0	35.0	39.0	32.0	39.0
14	37.86743064429696	39.0	37.0	40.0	33.0	41.0
15	37.77509377962818	40.0	36.0	41.0	33.0	41.0
16	37.84428459540789	40.0	36.0	41.0	33.0	41.0
17	37.58170163650604	39.0	36.0	41.0	32.0	41.0
18	37.22669936930624	39.0	36.0	40.0	32.0	41.0
19	37.027598004864174	39.0	36.0	40.0	31.0	41.0
20	36.970155406240984	39.0	35.0	40.0	32.0	41.0
21	36.94198029597263	38.0	35.0	40.0	32.0	41.0
22	36.862731357434356	38.0	35.0	40.0	32.0	41.0
23	36.85279690011954	38.0	35.0	40.0	33.0	41.0
24	36.4785028236943	38.0	35.0	40.0	31.0	41.0
25	36.403479121150916	38.0	35.0	40.0	31.0	41.0
26	36.11610124077662	37.0	34.0	40.0	31.0	41.0
27	36.13331134836555	38.0	35.0	40.0	31.0	41.0
28	36.05808153674925	38.0	34.0	40.0	31.0	41.0
29	35.99960839276145	38.0	34.0	40.0	31.0	41.0
30	35.7758357722907	37.0	34.0	40.0	30.0	41.0
31	35.57628096788821	37.0	34.0	40.0	29.0	41.0
32	35.36269013561977	37.0	34.0	40.0	29.0	41.0
33	35.05983346386908	37.0	34.0	40.0	25.0	41.0
34	34.53031864462674	37.0	33.0	40.0	24.0	41.0
35	33.95488272393751	37.0	33.0	40.0	21.0	41.0
36	33.62102724761944	37.0	33.0	40.0	18.0	41.0
37	33.28842903664619	37.0	33.0	40.0	16.0	41.0
38	33.110062244940025	36.0	33.0	40.0	15.0	41.0
39	32.66948349066326	36.0	31.0	40.0	15.0	41.0
40	32.2747846160188	35.0	30.0	40.0	13.0	41.0
41	32.073663382662104	35.0	30.0	40.0	12.0	41.0
42	31.870769611278288	35.0	30.0	40.0	10.0	41.0
43	31.801290242796487	35.0	30.0	40.0	10.0	41.0
44	31.67952100251453	35.0	30.0	39.0	10.0	41.0
45	31.546415763221898	35.0	30.0	39.0	10.0	41.0
46	31.075559586132982	35.0	27.0	39.0	9.0	41.0
47	30.702770105940065	35.0	25.0	39.0	9.0	40.0
48	30.395997361803868	35.0	24.0	39.0	8.0	40.0
49	30.1698957088091	35.0	24.0	39.0	8.0	40.0
50	30.01040850818253	35.0	24.0	39.0	8.0	40.0
51	27.205676243868254	32.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	4.0
12	0.0
13	0.0
14	3.0
15	3.0
16	2.0
17	11.0
18	42.0
19	72.0
20	107.0
21	183.0
22	295.0
23	489.0
24	781.0
25	1149.0
26	1490.0
27	1721.0
28	1765.0
29	1805.0
30	1956.0
31	2386.0
32	2848.0
33	3615.0
34	4096.0
35	4853.0
36	4811.0
37	6042.0
38	5009.0
39	2974.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.8791376396389	13.528999546560039	12.96838286821386	25.623479945587203
2	34.912815862154254	39.87386124737211	13.61968753864545	11.593635351828189
3	18.430273300630695	39.37095510944392	32.80019786471001	9.398573725215384
4	17.438888659878806	16.7257512675708	54.237602539263776	11.597757533286615
5	26.060431180180547	27.163114720309988	30.174368275691492	16.602085823817962
6	15.010923780864834	36.48542808854446	37.86017560493013	10.64347252566058
7	28.785193124201324	40.62615936353518	26.493260233315468	4.095387278948019
8	41.537161465847724	19.065089245228574	31.400717259573767	7.997032029349932
9	39.098891133187685	8.848262500515272	29.290160352858734	22.76268601343831
10	26.697308215507647	18.601343831155447	38.398120285254954	16.30322766808195
11	20.400676037759183	20.221361144317573	40.27783503029803	19.100127787625212
12	19.51646811492642	17.092625417370872	45.21414732676533	18.176759140937385
13	14.339008203141102	20.05441279525125	51.97658600931613	13.62999299229152
14	13.269302114679087	27.8618244775135	37.89109196586834	20.977781441939076
15	10.097283482418897	22.764747104167526	54.04386001071767	13.094109402695905
16	11.709056432664164	28.01846737293376	36.07114885197246	24.201327342429614
17	11.752339337977658	24.69392802671174	44.17123541778309	19.382497217527515
18	11.319510284842739	28.26373716971021	42.340986850241144	18.075765695205902
19	11.744094975060802	29.218022177336245	35.66511397831732	23.372768869285625
20	12.706624345603693	28.811987303681107	45.16880333072262	13.312585019992579
21	14.093738406364647	32.15095428500763	39.22667875839895	14.528628550228781
22	11.529741539222558	23.187270703656377	38.723772620470754	26.559215136650316
23	12.995177047693641	30.528875881116285	41.73502617585226	14.740920895337814
24	19.974030256811908	21.616719567995382	34.803578053505916	23.605672121686798
25	12.448988004451957	36.269013561977	33.56074034378994	17.72125808978111
26	13.681520260521868	21.173585061214396	39.34416092996414	25.8007337482996
27	19.469063028154498	25.281338884537696	34.48823117193619	20.761366915371614
28	13.221897027907167	22.03718207675502	41.56807782678594	23.172843068551877
29	19.172265963147698	19.46494084669607	32.996001483985324	28.366791706170908
30	13.730986438023002	25.652335215796203	42.77175481264685	17.844923533533947
31	22.704975473020323	19.260892864503894	34.570674801104744	23.46345686137104
32	18.562183107300385	25.47302032235459	32.73836514283359	23.22643142751144
33	13.801063522816273	21.5301537573684	33.746238509419186	30.92254421039614
34	16.325899666103304	21.152974153922255	39.87592233810132	22.64520384187312
35	11.49882517828435	28.614122593676573	36.74924770188384	23.137804526155243
36	19.17844923533534	29.459169792654272	32.24782554928068	19.11455542272971
37	12.475782183931736	25.11439053547137	42.23587122305124	20.173956057545652
38	16.56910837215054	27.738159033760667	28.66977204336535	27.02296055072344
39	19.102188878354426	23.139865616884457	38.38575374087967	19.372191763881446
40	15.14077249680531	27.59800486417412	37.04810585761985	20.213116781400718
41	13.679459169792654	34.24090028443052	34.224411558596806	17.855228987180016
42	14.872830702007503	26.769446391030133	41.941135248773655	16.416587658188714
43	21.051980708190772	26.58807040685931	30.34543880621625	22.014510078733664
44	19.05272270085329	22.21855806092584	35.30442310070489	23.424296137515974
45	16.257883672039245	20.992209077043572	34.53975844016654	28.210148810750653
46	24.314687332536376	27.19609217197741	30.48765406653201	18.001566428954202
47	13.079681767591408	21.474504307679624	46.13133270126551	19.314481223463456
48	19.172265963147698	24.063234263572284	31.464611072179398	25.29988870110062
49	14.516262005853498	20.149222968795087	43.559091471206564	21.775423554144854
50	16.579413825796614	19.687538645451173	39.06385259079105	24.66919493796117
51	16.973082155076465	19.578300836802836	34.08219629828105	29.366420709839648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	509.0
1	455.0
2	401.0
3	2208.0
4	4015.0
5	2649.5
6	1284.0
7	1032.5
8	781.0
9	730.0
10	679.0
11	632.0
12	585.0
13	597.5
14	610.0
15	551.5
16	493.0
17	466.5
18	440.0
19	422.0
20	404.0
21	399.0
22	394.0
23	395.5
24	397.0
25	465.5
26	518.0
27	502.0
28	595.5
29	689.0
30	684.5
31	680.0
32	749.5
33	819.0
34	864.5
35	910.0
36	955.5
37	1001.0
38	1046.5
39	1092.0
40	1204.0
41	1316.0
42	1598.5
43	1881.0
44	3287.0
45	4693.0
46	7171.0
47	9649.0
48	6597.0
49	3545.0
50	3235.0
51	2925.0
52	2265.0
53	1605.0
54	1438.0
55	1271.0
56	1146.0
57	1021.0
58	972.0
59	923.0
60	846.0
61	769.0
62	680.5
63	592.0
64	503.0
65	414.0
66	343.0
67	272.0
68	229.0
69	186.0
70	143.0
71	100.0
72	82.5
73	65.0
74	44.5
75	23.0
76	22.0
77	15.5
78	9.0
79	9.0
80	9.0
81	5.5
82	2.0
83	2.0
84	2.0
85	2.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	48518.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.585390988911335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.59906291834002	55.79578713054949
2	6.1546184738955825	7.580691702048724
3	1.5763052208835342	2.9123212003792407
4	0.5321285140562249	1.3108537037800403
5	0.26439089692101736	0.8141308380394905
6	0.18406961178045514	0.680159940640587
7	0.107095046854083	0.46168432334391357
8	0.07028112449799197	0.3462632425079352
9	0.04350736278447122	0.24114761531802628
>10	0.33801874163319945	3.8830949338389873
>50	0.04685408299866131	2.0054412795251246
>100	0.05689424364123159	7.929016035285873
>500	0.01673360107095047	7.797106228616184
>1k	0.010040160642570281	8.242301826126386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1545	3.184385176635476	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	1230	2.535141596933097	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1224	2.522775052557814	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	863	1.7787212993115957	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	837	1.7251329403520341	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	774	1.5952842244115588	No Hit
GAATCTATCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	764	1.5746733171194196	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	545	1.1232944474215756	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	439	0.904818830124902	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.6780988499113731	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	318	0.6554268518900201	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	283	0.5832886763675337	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	274	0.5647388598046086	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	268	0.5523723154293252	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	267	0.5503112247001113	No Hit
GAATGAATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	232	0.47817304917762476	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	225	0.46374541407312747	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176	0.3627519683416464	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	171	0.35244651469557686	No Hit
GCTGTCACTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	168	0.3462632425079352	No Hit
GAACTGACTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	161	0.3318356074034379	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTT	152	0.31328579084051283	No Hit
GAATCTATCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTT	143	0.2947359742775877	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	122	0.2514530689640958	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	119	0.2452697967764541	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	99	0.20404798219217612	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.1958036192753205	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	94	0.1937425285461066	No Hit
GAATGCAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	77	0.1587039861494703	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.144276351044973	No Hit
CCTGTCACTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	68	0.1401541695865452	No Hit
GAATAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	65	0.1339708973989035	No Hit
GCTGACTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	64	0.1319098066696896	No Hit
GAATGAAACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	63	0.1298487159404757	No Hit
GAATTATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	60	0.12366544375283399	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57	0.1174821715651923	No Hit
GAACAGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	56	0.11542108083597839	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	54	0.1112988993775506	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.10511562718990891	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1999258007337483	0.0
2	0.0	0.0	0.0	1.3953584236778103	0.0
3	0.0	0.0	0.0	1.6365060389958366	0.0
4	0.0	0.0	0.0	2.592852137351086	0.0
5	0.0	0.0	0.0	6.682056144111463	0.0
6	0.0	0.0	0.0	7.62603569809143	0.0
7	0.0	0.0	0.0	9.211014468856918	0.0
8	0.0	0.0	0.0	11.377220825260729	0.0
9	0.0	0.0	0.0	11.956387320169833	0.0
10	0.0	0.0	0.0	18.283935858856506	0.0
11	0.0	0.0	0.0	19.192876870439836	0.0
12	0.0	0.0	0.0	23.442845954078898	0.0
13	0.0	0.0	0.0	23.912774640339666	0.0
14	0.0	0.0	0.0	24.26728224576446	0.0
15	0.0	0.0	0.0	25.042252359948886	0.0
16	0.0	0.0	0.0	25.559586132981575	0.0
17	0.0	0.0	0.0	26.02127045632549	0.0
18	0.0	0.0	0.0	26.491199142586257	0.0
19	0.0	0.0	0.0	26.86425656457397	0.0
20	0.0	0.0	0.0	27.146625994476278	0.0
21	0.0	0.0	0.0	27.472278329692074	0.0
22	0.0	0.0	0.0	27.843274660950573	0.0
23	0.0	0.0	0.0	28.203965538563008	0.0
24	0.0	0.0	0.0	28.4698462426316	0.0
25	0.0	0.0	0.0	28.673894224823776	0.0
26	0.0	0.0	0.0	28.968630199101366	0.0
27	0.0	0.0	0.0	29.228327630982317	0.0
28	0.0	0.0	0.0	29.477719609217196	0.0
29	0.0	0.0	0.0	29.698256317243086	0.0
30	0.0	0.0	0.0	29.97650356568696	0.0
31	0.0	0.0	0.0	30.201162455171275	0.0
32	0.0	0.0	0.0	30.479409703615154	0.0
33	0.0	0.0	0.0	30.726740591120823	0.0
34	0.0	0.0	0.0	31.0049878395647	0.0
35	0.0	0.0	0.0	31.246135454882722	0.0
36	0.0	0.0	0.0	31.485221979471536	0.0
37	0.0	0.0	0.0	31.709880868955853	0.0
38	0.0	0.0	0.0	31.99431138958737	0.0
39	0.0	0.0	0.0	32.21690918834247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGTCA	30	2.1226515E-6	45.000004	1
GAATATG	30	2.1226515E-6	45.000004	1
AACGGCT	60	0.0	45.000004	7
AAACGGC	75	0.0	45.000004	6
TGAAACG	75	0.0	45.000004	4
AATCTAT	85	0.0	45.0	2
CGTTATT	85	0.0	45.0	1
AACTGAC	35	1.1800694E-7	45.0	2
ATCTATC	85	0.0	45.0	3
ATGAAAC	160	0.0	45.0	3
AAGGGGC	25	3.8356324E-5	45.0	5
TACAAGG	20	6.966592E-4	45.0	2
TATCTCT	85	0.0	45.0	6
GAACTGA	35	1.1800694E-7	45.0	1
AAAGGGT	20	6.966592E-4	45.0	4
GAATCTG	185	0.0	45.0	1
GAATCTA	90	0.0	45.0	1
ATATGTC	25	3.8356324E-5	45.0	3
TTAGGAG	20	6.966592E-4	45.0	5
CACTAGG	20	6.966592E-4	45.0	3
>>END_MODULE