##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	121234
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2742052559513	31.0	31.0	33.0	28.0	34.0
2	31.450574921226718	31.0	31.0	34.0	30.0	34.0
3	30.61552039856806	31.0	30.0	34.0	26.0	34.0
4	34.86903014005972	35.0	35.0	37.0	32.0	37.0
5	31.526560205882838	35.0	35.0	37.0	0.0	37.0
6	33.50110530049326	35.0	35.0	37.0	17.0	37.0
7	21.345398155632907	32.0	0.0	37.0	0.0	37.0
8	28.303982381180198	35.0	17.0	37.0	17.0	37.0
9	34.475625649570254	35.0	32.0	39.0	28.0	39.0
10	35.991594767144534	37.0	35.0	39.0	32.0	39.0
11	36.48799016777472	37.0	35.0	39.0	32.0	39.0
12	36.922546480360296	39.0	37.0	39.0	33.0	39.0
13	36.725638022336966	39.0	35.0	39.0	33.0	39.0
14	37.96092680271211	40.0	37.0	41.0	33.0	41.0
15	38.078946500156725	40.0	37.0	41.0	33.0	41.0
16	38.14783806522922	40.0	37.0	41.0	33.0	41.0
17	38.08382961875382	40.0	37.0	41.0	33.0	41.0
18	37.878152993384695	39.0	37.0	40.0	33.0	41.0
19	37.71510467360641	39.0	37.0	40.0	33.0	41.0
20	37.48148209248231	39.0	35.0	40.0	33.0	41.0
21	37.3431710576241	39.0	35.0	40.0	33.0	41.0
22	37.23000148473201	39.0	35.0	40.0	33.0	41.0
23	37.12560007918571	38.0	35.0	40.0	33.0	41.0
24	36.90460596862266	38.0	35.0	40.0	32.0	41.0
25	36.73664978471386	38.0	35.0	40.0	32.0	41.0
26	36.562474223402674	38.0	35.0	40.0	32.0	41.0
27	36.487858191596416	38.0	35.0	40.0	31.0	41.0
28	36.348986257980435	38.0	35.0	40.0	31.0	41.0
29	36.27757064849795	38.0	35.0	40.0	31.0	41.0
30	36.003777818103835	38.0	34.0	40.0	30.0	41.0
31	35.812296880413086	38.0	34.0	40.0	30.0	41.0
32	35.64572644637643	38.0	34.0	40.0	29.0	41.0
33	35.18656482504908	38.0	34.0	40.0	25.0	41.0
34	34.668492337133145	37.0	33.0	40.0	24.0	41.0
35	34.22456571588828	37.0	33.0	40.0	21.0	41.0
36	33.75927545078113	37.0	33.0	40.0	18.0	41.0
37	33.50090733622581	37.0	33.0	40.0	16.0	41.0
38	33.180229968490686	37.0	33.0	40.0	15.0	41.0
39	32.9151558143755	37.0	32.0	40.0	15.0	41.0
40	32.65508850652457	37.0	31.0	40.0	12.0	41.0
41	32.41304419552271	36.0	31.0	40.0	10.0	41.0
42	32.12183050959302	36.0	30.0	40.0	10.0	41.0
43	31.81782338287939	36.0	30.0	40.0	10.0	41.0
44	31.462815711764026	35.0	29.0	40.0	9.0	41.0
45	31.30006433838692	35.0	28.0	40.0	9.0	41.0
46	30.95809756338981	35.0	26.0	39.0	8.0	41.0
47	30.759762112938613	35.0	25.0	39.0	8.0	41.0
48	30.462164079383673	35.0	24.0	39.0	8.0	41.0
49	30.170933896431695	35.0	24.0	39.0	8.0	41.0
50	29.887267598198527	35.0	23.0	39.0	8.0	41.0
51	27.729943745154	33.0	19.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	4.0
10	3.0
11	2.0
12	1.0
13	3.0
14	0.0
15	8.0
16	9.0
17	14.0
18	55.0
19	110.0
20	207.0
21	435.0
22	691.0
23	1229.0
24	2020.0
25	3052.0
26	3794.0
27	4221.0
28	4204.0
29	4183.0
30	4471.0
31	5040.0
32	5949.0
33	8067.0
34	9436.0
35	11700.0
36	12720.0
37	16821.0
38	14331.0
39	8449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.38667370539618	18.45191942854315	21.004833627530232	27.156573238530445
2	41.25905274098025	21.943514195687676	21.451902931520863	15.345530131811207
3	27.050167444776218	22.206641701172938	36.936008050546874	13.807182803503967
4	24.456835541184816	21.569856640876324	38.493326954484715	15.479980863454145
5	18.687826847254073	31.55880363594371	33.57473975947342	16.178629757328803
6	19.907781645413003	27.893990134780672	38.70696339310754	13.491264826698782
7	37.531550555124795	41.053664813501165	17.23031492815547	4.18446970321857
8	60.631506013164625	6.077503010706567	26.20139564808552	7.089595328043289
9	54.63978751835293	7.735453750598017	29.18158272431826	8.443176006730784
10	30.093868056815744	21.269610835244237	34.9316198426184	13.70490126532161
11	21.168978999290626	21.1541316792319	39.410561393668445	18.26632792780903
12	19.514327663856673	17.760694194697855	44.54278502730257	18.182193114142898
13	18.252305458864676	18.49728623983371	46.16196776481845	17.088440536483166
14	15.093950541927182	21.89649768216837	44.23016645495488	18.77938532094957
15	13.54240559578996	22.86569774155765	46.741013247108896	16.850883415543493
16	16.998531765016413	23.46288994836432	41.7811834963789	17.75739479024036
17	16.13986175495323	23.592391573321013	40.55215533596186	19.715591335763893
18	15.918801656301037	23.325964663378258	42.78585215368626	17.969381526634443
19	15.948496296418496	24.988864509955953	39.20269891284623	19.85994028077932
20	15.81239586254681	26.753221043601634	39.684411963640564	17.749971130210998
21	16.534140587623934	26.040549680782622	40.51998614250128	16.90532358909217
22	14.892686870019961	23.46288994836432	39.4905719517627	22.15385122985301
23	14.778857416236368	23.849745121005657	40.8697230149958	20.501674447762177
24	17.25011135490044	22.356764603988978	39.59367834105944	20.79944570005114
25	16.195126779616277	24.08317798637346	38.410016991932956	21.311678242077306
26	14.681524984740255	22.743619776630318	40.59009848722306	21.98475675140637
27	16.752726132933006	22.33119421944339	39.642344556807494	21.273735090816107
28	14.489334675091145	22.800534503522112	40.92663774188759	21.78349307949915
29	16.590230463401355	21.84948116864906	41.08665885807612	20.47362950987347
30	16.968012273784584	22.430176353168253	39.38911526469472	21.212696108352443
31	15.456060181137305	22.143953016480523	39.92691819126647	22.473068611115693
32	15.90230463401356	21.135984954715674	40.46802052229573	22.49368988897504
33	14.882788656647476	21.964135473547024	39.606051107775045	23.547024762030453
34	13.091212036227462	21.186300872692478	42.478182688024816	23.24430440305525
35	13.525908573502482	22.079614629559362	38.887605787155415	25.506871009782735
36	14.848969760958147	23.591566722206643	36.79743306333207	24.762030453503144
37	14.048864180015508	23.149446524902256	38.250820726858805	24.550868568223436
38	14.401075605853144	24.89400663180296	36.04516884702311	24.659748915320783
39	16.587755910058235	22.654535856277942	35.311876206344756	25.44583202731907
40	17.65016414537176	21.906395895540857	35.96515828892885	24.478281670158538
41	15.14261675767524	22.815381823580843	33.90055594965109	28.14144546909283
42	16.215748057475626	22.448323077684478	36.3734595905439	24.96246927429599
43	17.550357160532524	22.370787072933336	36.77516208324397	23.303693683290167
44	17.566854182820002	22.55060461586684	36.11033208505865	23.772209116254515
45	17.811834963789035	21.771120312783545	34.705610637279975	25.71143408614745
46	19.07303231766666	22.515136017948763	34.78892059983173	23.62291106455285
47	15.971592127620966	22.30149957932593	36.95580447729185	24.771103815761254
48	15.76290479568438	22.57452529818368	35.48014583367702	26.182424072454925
49	16.949865549268356	20.91492485606348	37.811175082897535	24.324034511770627
50	16.006235874424664	20.043057228170316	38.174934424336406	25.77577247306861
51	14.773908309550126	19.857465727436196	36.380058399458896	28.988567563554778
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	294.0
1	368.0
2	442.0
3	4365.0
4	8288.0
5	5428.0
6	2568.0
7	2156.0
8	1744.0
9	1673.0
10	1602.0
11	1536.5
12	1471.0
13	1396.0
14	1321.0
15	1272.5
16	1224.0
17	1161.5
18	1099.0
19	1054.5
20	1010.0
21	958.5
22	907.0
23	950.0
24	993.0
25	1083.5
26	1310.0
27	1446.0
28	1592.0
29	1738.0
30	1848.5
31	1959.0
32	2228.0
33	2497.0
34	2643.5
35	2790.0
36	2915.5
37	3041.0
38	3234.0
39	3427.0
40	3828.0
41	4229.0
42	4914.0
43	5599.0
44	6939.0
45	8279.0
46	10335.0
47	12391.0
48	12683.0
49	12975.0
50	12032.0
51	11089.0
52	9063.5
53	7038.0
54	5902.0
55	4766.0
56	4174.0
57	3582.0
58	3227.0
59	2872.0
60	2539.5
61	2207.0
62	1921.5
63	1636.0
64	1425.0
65	1214.0
66	994.5
67	775.0
68	688.5
69	602.0
70	487.5
71	373.0
72	321.5
73	270.0
74	198.0
75	101.0
76	76.0
77	62.0
78	48.0
79	36.0
80	24.0
81	18.5
82	13.0
83	10.0
84	7.0
85	6.5
86	6.0
87	3.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	121234.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.81234637147995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3293788176145	65.3331573650956
2	8.29676508853558	12.414009271326526
3	2.407991355928466	5.404424501377501
4	0.9040993186178305	2.705511655146246
5	0.38258837019559416	1.4311166834386393
6	0.18964034488081324	0.8512463500338189
7	0.10584577388696555	0.5542999488592308
8	0.07938433041522415	0.47511424187934076
9	0.055128007232794554	0.37118300146823496
>10	0.21389666806324284	3.082468614415098
>50	0.017640962314494257	0.8957883102100072
>100	0.014333281880526584	1.7998251315637526
>500	0.001102560144655891	0.5625484600029694
>1k	0.002205120289311782	4.119306465183034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3210	2.6477720771400763	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1784	1.4715343880429583	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.5625484600029694	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	334	0.27550027220086776	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	246	0.20291337413596844	TruSeq Adapter, Index 19 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.19631456522097762	No Hit
CCTGTCACTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	196	0.16167081841727568	TruSeq Adapter, Index 19 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	192	0.15837141395978027	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	145	0.11960341158420906	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	141	0.11630400712671363	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	125	0.10310638929673195	TruSeq Adapter, Index 19 (95% over 22bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.10145668706798422	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.10145668706798422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26230265437088607	0.0
2	0.0	0.0	0.0	1.021990530709207	0.0
3	0.0	0.0	0.0	1.3263605919131596	0.0
4	0.0	0.0	0.0	1.717340020126367	0.0
5	0.0	0.0	0.0	3.053598825412013	0.0
6	0.0	0.0	0.0	4.32799379711962	0.0
7	0.0	0.0	0.0	5.668376857977135	0.0
8	0.0	0.0	0.0	8.322747744032203	0.0
9	0.0	0.0	0.0	9.738192256297738	0.0
10	0.0	0.0	0.0	11.988386096309616	0.0
11	0.0	0.0	0.0	14.286421300955178	0.0
12	0.0	0.0	0.0	16.191827375158784	0.0
13	0.0	0.0	0.0	17.083491429796922	0.0
14	0.0	0.0	0.0	17.62459376082617	0.0
15	0.0	0.0	0.0	18.150023920682315	0.0
16	0.0	0.0	0.0	18.974875035056172	0.0
17	0.0	0.0	0.0	19.930052625501098	0.0
18	0.0	0.0	0.0	21.061748354422026	0.0
19	0.0	0.0	0.0	21.737301417094216	0.0
20	0.0	0.0	0.0	22.40213141527954	0.0
21	0.0	0.0	0.0	23.126350693699788	0.0
22	0.0	0.0	0.0	23.89098767672435	0.0
23	0.0	0.0	0.0	24.612732401801473	0.0
24	0.0	0.0	0.0	25.203325799693154	0.0
25	0.0	0.0	0.0	25.748552386294275	0.0
26	0.0	0.0	0.0	26.27233284392167	0.0
27	0.0	0.0	0.0	26.805186663807184	0.0
28	0.0	0.0	0.0	27.287724565715887	0.0
29	0.0	0.0	0.0	27.789234043255192	0.0
30	0.0	0.0	0.0	28.39220020786248	0.0
31	0.0	0.0	0.0	28.887110876486794	0.0
32	0.0	0.0	0.0	29.374597885081744	0.0
33	0.0	0.0	0.0	29.923948727254732	0.0
34	0.0	0.0	0.0	30.42628305590841	0.0
35	0.0	0.0	0.0	30.962436280251413	0.0
36	0.0	0.0	0.0	31.4235280531864	0.0
37	0.0	0.0	0.0	31.887094379464507	0.0
38	0.0	0.0	0.0	32.400976623719416	0.0
39	0.0	0.0	0.0	32.99321972383985	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	20	7.008311E-4	45.0	14
CGTGGCG	20	7.008311E-4	45.0	27
TAGAGGA	20	7.008311E-4	45.0	4
GCGACCT	20	7.008311E-4	45.0	11
TGCCAGT	35	1.1999327E-7	45.0	24
CACGGGC	25	3.870118E-5	45.0	4
GGGTACC	40	6.728442E-9	45.0	7
GACCGAT	20	7.008311E-4	45.0	9
TGGCGAC	20	7.008311E-4	45.0	29
GCAGTCT	20	7.008311E-4	45.0	5
CGACCTC	20	7.008311E-4	45.0	12
ACCTGGT	40	6.728442E-9	45.0	11
CGCTGTA	20	7.008311E-4	45.0	1
AGGCGAT	45	3.783498E-10	45.0	7
CCTCGAT	20	7.008311E-4	45.0	15
ACGGGCT	20	7.008311E-4	45.0	5
GTAGCAT	35	1.1999327E-7	45.0	29
GGGGTCT	20	7.008311E-4	45.0	7
CCTCAAG	20	7.008311E-4	45.0	24
TGCCTCA	20	7.008311E-4	45.0	22
>>END_MODULE